• 제목/요약/키워드: Single fusion

검색결과 557건 처리시간 0.026초

Construction of Astaxanthin Overproducing Strain of Phaffia rhodozyma by Protoplast Fusion

  • Koh, Moo-Suk;Kim, Sang-Moon
    • Journal of Microbiology and Biotechnology
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    • 제2권1호
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    • pp.46-49
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    • 1992
  • The availability of Phaffia rhodozyma as an astaxanthin sources in the aquaculture industry is limited because of the low carotenoid content of natural isolate. In this study, we have used the protoplast fusion technique to construct cell hybrids with an increased content of astaxanthin from P. rhodozyma. Cell hybrids (F307 and F406) obtained were very stable and produced considerably more astaxanthin (> 1 mg/g yeast) than the wild parent. Karyogamy was confirmed by the isolation of recombinants after mitotic segregation of parental auxotrophic genetic markers, the increased amount of chromosomal DNA/cell and the presence of single nucleus/cell.

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Reducing Spectral Signature Confusion of Optical Sensor-based Land Cover Using SAR-Optical Image Fusion Techniques

  • ;Tateishi, Ryutaro;Wikantika, Ketut;M.A., Mohammed Aslam
    • 대한원격탐사학회:학술대회논문집
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    • 대한원격탐사학회 2003년도 Proceedings of ACRS 2003 ISRS
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    • pp.107-109
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    • 2003
  • Optical sensor-based land cover categories produce spectral signature confusion along with degraded classification accuracy. In the classification tasks, the goal of fusing data from different sensors is to reduce the classification error rate obtained by single source classification. This paper describes the result of land cover/land use classification derived from solely of Landsat TM (TM) and multisensor image fusion between JERS 1 SAR (JERS) and TM data. The best radar data manipulation is fused with TM through various techniques. Classification results are relatively good. The highest Kappa Coefficient is derived from classification using principal component analysis-high pass filtering (PCA+HPF) technique with the Overall Accuracy significantly high.

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지형 정보를 사용한 다중 지상 표적 추적 알고리즘의 연구 (Study on Multiple Ground Target Tracking Algorithm Using Geographic Information)

  • 김인택;이응기
    • 제어로봇시스템학회논문지
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    • 제6권2호
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    • pp.173-180
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    • 2000
  • During the last decade many researches have been working on multiple target tracking problem in the area of radar application, Various approaches have been proposed to solve the tracking problem and the concept of sensor fusion was established as an effort. In this paper utilization of geographic information for ground target tracking is investigated and performance comparison with the results of applying sensor fusion is described. Geographic information is used in three aspects: association masking target measurement and re-striction of removing true target. Simulation results indicate that using two sensors shows better performance with respect to tracking but a single with geographic information is a winner in reducing the number of false tracks.

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오류 역전파 신경망 기반의 센서융합을 이용한 이동로봇의 효율적인 지도 작성 (An Effective Mapping for a Mobile Robot using Error Backpropagation based Sensor Fusion)

  • 김경동;곡효천;최경식;이석규
    • 한국정밀공학회지
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    • 제28권9호
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    • pp.1040-1047
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    • 2011
  • This paper proposes a novel method based on error back propagation neural networks to fuse laser sensor data and ultrasonic sensor data for enhancing the accuracy of mapping. For navigation of single robot, the robot has to know its initial position and accurate environment information around it. However, due to the inherent properties of sensors, each sensor has its own advantages and drawbacks. In our system, the robot equipped with seven ultrasonic sensors and a laser sensor navigates to map two different corridor environments. The experimental results show the effectiveness of the heterogeneous sensor fusion using an error backpropagation algorithm for mapping.

Purification of Single Chain Human Insulin Precursors Using Various Fusion Proteins

  • 박선호;조정우;남두현
    • 한국생물공학회:학술대회논문집
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    • 한국생물공학회 2000년도 추계학술발표대회 및 bio-venture fair
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    • pp.619-622
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    • 2000
  • For the production of $B^{30}-homoserine$ human insulin precursor, four types of fusion peptides LacZ, MBP, GST, and His-tagged sequence were studied in this work. Recombinant E. coli JM 103 and E. coli JM 109 containing fusion peptides were cultivated at $37^{\circ}C$ for 1hr, and gene expression was occurred when 0.5mM of isopropyl-D-thiogalactoside(IPTG) was added to the culture broth, and followed by longer than 4hr fermentation respectively. DEAE-Sphacel and gel filtration chromatography, amylose and glutathione-Sepharose 4B affinity chromatography, and nickel-affinity chromatography system were employed as purification of $B^{30}-homoserine$ human insulin precursor. Recovery yields of His-tagged, LacZ, GST, and MBP fused $B^{30}-homoserine$ human insulin precursor resulted in 47%, 20%, 20%, and 18%, respectively.

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Rao-Blackwellized Multiple Model Particle Filter자료융합 알고리즘 (Rao-Blackwellized Multiple Model Particle Filter Data Fusion algorithm)

  • 김도형
    • 한국항행학회논문지
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    • 제15권4호
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    • pp.556-561
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    • 2011
  • 일반적으로 비선형 시스템에서 particle filter가 Kalman Filter보다 표적추적 성능이 뛰어나다고 알려져 있다. 그러나 particle filter는 많은 연산량을 요구하는 단점이 있다. 본 논문에서는 particle filter 보다 적은 particle의 수, 즉 적은 연산량으로 동일한 성능을 가지는 Rao-Blackwellized particle filter의 모델의 민감성을 줄인 Rao-Blackwellized Multiple Model Particle Filter(RBMMPF)의 알고리즘을 소개하고 이에 다중센서 정보를 융합하는 자료융합 기법을 적용하였다. 시뮬레이션을 통해 단일센서 정보를 이용한 RBMMPF 표적추적 성능과 다중센서정보를 융합한 RBMMPF의 표적추적 성능을 비교, 분석하였다.

무인잠수정의 수중합법을 위한 센서융합 (Sensor Fusion for Underwater Navigation of Unmanned Underwater Vehicle)

  • 서주노
    • 한국군사과학기술학회지
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    • 제8권4호
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    • pp.14-23
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    • 2005
  • In this paper we propose a sensor fusion method for the navigation algorithm which can be used to estimate state vectors such as position and velocity for its motion control using multi-sensor output measurements. The output measurement we will use in estimating the state is a series of known multi-sensor asynchronous outputs with measurement noise. This paper investigates the Extended Kalman Filtering method to merge asynchronous heading, heading rate, velocity of DVL, and SSBL information to produce a single state vector. Different complexity of Kalman Filter, with. biases and measurement noise, are investigated with theoretically data from MOERI's SAUV. All levels of complexity of the Kalman Filters are shown to be much more close and smooth to real trajectories then the basic underwater acoustic navigation system commonly used aboard underwater vehicle.

정맥패턴 융합을 위한 Boundary Stitching Algorithm (Boundary Stitching Algorithm for Fusion of Vein Pattern)

  • 임영규;장경식
    • 한국정보처리학회:학술대회논문집
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    • 한국정보처리학회 2005년도 춘계학술발표대회
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    • pp.521-524
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    • 2005
  • This paper proposes a fusion algorithm which merges multiple vein pattern images into a single image, larger than those images. As a preprocessing step of template matching, during the verification of biometric data such as fingerprint image, vein pattern image of hand, etc., the fusion technique is used to make reference image larger than the candidate images in order to enhance the matching performance. In this paper, a new algorithm, called BSA (Boundary Stitching Algorithm) is proposed, in which the boundary rectilinear parts extracted from the candidate images are stitched to the reference image in order to enlarge its matching space. By applying BSA to practical vein pattern verification system, its verification rate was increased by about 10%.

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Renal and Ureteral Fusion in a Calf with Atresia Ani

  • Jeong, Won-Il;Lee, Cha-Soo;Kim, Seok-Jae;Kim, Jin-Hyun;Jeong, Kyu-Shik
    • 한국수의병리학회:학술대회논문집
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    • 한국수의병리학회 2002년도 추계학술대회초록집
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    • pp.138-138
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    • 2002
  • A 10-day old male calf exhibited multiple congenital anomalies of the urinary and gastrointestinal tracts, including renal fusion (horseshoe kidney), ureteral fusion, rectovesicular fistula, and atresia ani. The single kidney was fused at the caudal poles. The left kidney and cranial half of right kidney were shrunken, while the remaining lobules were hypertrophic. Ureters were fused cranially and bifurcated caudally. The terminal rectum was narrowed and connected with the bladder. The anus was imperforate. The cause of these anomalies could not be determined. This is the first report of this constellation of congenital anomalies in a calf.

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Regulation of fpr Gene Encoding NADPH : Ferredoxin Oxidoreductase by the soxRS Locus in Escherichia coli

  • Koh, Young-Sang;Choih, Jenny;Roe, Jung-Hye
    • Journal of Microbiology
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    • 제34권2호
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    • pp.137-143
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    • 1996
  • We isolated a promoter inducible by paraquat, a superoxide-generating agent, from Escherichia coli using a promoter-probing plasmid pRS415. From sequence analysis we found out the promoter is for fpr ENCODING nadph : ferredoxin oxidoreductase. We constructed on operon fusion of lacZ gene with fpr promoter to monitor the expression of the gene in the single-copy state. LacZ expression generators, menadione and plumbagin, also induced the expression of .betha.-galactosidase in the fusion strain. On the other hand, no significant induction was observed by treatment with hydrogen peroxide, ethanol, and heat shock. Induction of .betha.-galactosidase was significantly reduced by introducing a .DELTA. sox 8 :: cat of soxS3 :: Tn10 mutation into the fusion strain, indicating that fpr gene is a member of the soxRS regulon. The transcriptional start site was determined by primer extension analysis. Possible roles of fpr induction in superoxide stress were discussed.

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