• The Korean Journal of Physiology and Pharmacology
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• v.20 no.6
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• pp.657-667
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• 2016

Critical limb ischemia (CLI) is one of the most severe forms of peripheral artery diseases, but current treatment strategies do not guarantee complete recovery of vascular blood flow or reduce the risk of mortality. Recently, human bone marrow derived mesenchymal stem cells (MSCs) have been reported to have a paracrine influence on angiogenesis in several ischemic diseases. However, little evidence is available regarding optimal cell doses and injection frequencies. Thus, the authors undertook this study to investigate the effects of cell dose and injection frequency on cell survival and paracrine effects. MSCs were injected at $10^6$ or $10^5$ per injection (high and low doses) either once (single injection) or once in two consecutive weeks (double injection) into ischemic legs. Mice were sacrificed 4 weeks after first injection. Angiogenic effects were confirmed in vitro and in vivo, and M2 macrophage infiltration into ischemic tissues and rates of limb salvage were documented. MSCs were found to induce angiogenesis through a paracrine effect in vitro, and were found to survive in ischemic muscle for up to 4 weeks dependent on cell dose and injection frequency. In addition, double high dose and low dose of MSC injections increased vessel formation, and decreased fibrosis volumes and apoptotic cell numbers, whereas a single high dose did not. Our results showed MSCs protect against ischemic injury in a paracrine manner, and suggest that increasing injection frequency is more important than MSC dosage for the treatment CLI.

• Journal of the Korean GEO-environmental Society
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• v.10 no.6
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• pp.125-133
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• 2009

Rock fracturing technique through fluid injection into the wellbore has been widely used to extract geothermal heat and to enhance oil and gas production. Single fracture formation is ideal for the production. However, it is very difficult to form single fracture formation. Instead, the formation of segmented fracture is a common phenomenon. Therefore, design parameters are expected to be different from those of single fracture because of mechanical interaction between segmented fractures. In this paper, design parameters such as length, aperture, and net pressure are evaluated by using model of segmented fracture in which numerical technique is incorporated to consider mechanical interaction between segments. Results show that the existence of fracture segmentation affects design parameters in fracturing treatment in rock by fluid injection.

• Journal of Energy Engineering
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• v.14 no.3 s.43
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• pp.173-179
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• 2005

Fuel injection system has more benefits in power, fuel consumption and emission than carburetor system even in high speed small engine. Up to date fuel injection system is used in motor car but is not used in motorcycles in Korea. EFI (Electronic fuel injection) system which has ECU can control precise fuel supply to variable RPM in engine. The purpose of this study is the investigation of effects of fuel injection system to improve the engine performance and efficiency in variable revolution of high speed small engine which is 4 Valves SOHC single cylinder engine used in motorcycle.

• Transactions of the Korean Society of Automotive Engineers
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• v.8 no.6
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• pp.51-59
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• 2000

In-cylinder spray flow motion plays an important in the adjustment of mixture preparation with a fundamental spray characteristics and in-cylinder flow field well in direct-injection gasoline engine. In this study, the fundamental spray characteristics such as mean drop size, velocity distribution, spray angle were measured and in-cylinder spray flow motion was visualized in order to optimize intake port, piston top land and combustion chamber shapes in the development stage of mass-produced G야 engine. For these experiments, the PDPA measurements and Mie scattering technique were used for detailed spray characteristics and in-cylinder spray motions were obtained by use of ICCD camera through the single-cylinder optical engine. From the experimental results, the test injector shows a good low-end linearity between the dynamic flow and fuel injection pulse width and the fuel spray of 20mm or less in SMD with good spray symmetry. In addition, the in-cylinder tumble flow has more effect on the homogeneous mixture formation than that of in-cylinder swirl flow at early injection mode and the in-cylinder swirl flow plays a better role of stratified mixture preparation than tumble flow at late injection mode.

• Transactions of the Korean Society of Automotive Engineers
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• v.16 no.1
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• pp.181-187
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• 2008

Recently, alternative fuels are drawing more attentions due to the increasing need for lower emission characteristics and fuel consumption rate in automotive engines. The GTL(gas to luquid) is the one of most favored candidates. It has higher cetane number(more than 75) and almost negligible sulphur and aromatic contents. Therefore, enhanced emission characteristics are expected even in the application in diesel engines without any modification. In this study, the cylinder pressure and heat release, emission characteristics with fuel injection timings are compared between diesel and GTL fuel in the single cylinder diesel engine. Noticeable reduction in PM, THC and CO emission are observed due to lower sulphur and aromatic contents in GTL. Also, the ignition delay decreased due to higher cetane number of GTL, which slightly decreased the amount of NOx emissions. With the retards of main injection timing, NOx decreases more for the case of GTL, while the level of THC and CO emissions still remains lower than the case of diesel. Therefore, there is much room for the control of injection timing for NOx reduction without sacrificing THC and CO emissions. With the retards of main injection timing, Small size distribution of PM became lager and there amount increased. But from all conditions, size distribution of PM for the case GTL was lower than Diesel.

• Transactions of the Korean Society of Automotive Engineers
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• v.22 no.5
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• pp.108-115
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• 2014

In this study, experimental approaching method was applied under and single-cylinder engine to research the performance of direct needle-driven piezo injector (DPI) for CR direct-injection. As key-point factor of this DPI that relies on direct-acting operating of injector needle, unlike conventional hydraulic-servo, its nozzle needle can be directly driven by piezo actuator. Thus, effect of direct-acting injection of DPI on diesel combustion and emission characteristics was investigated under common-rail single-cylinder direct-injection engine, equipped with three different driving mechanism, including indirect-acting solenoid, piezo and DPI system. As main results, it found that a direct-acting piezo injector has higher of IMEP. And it has higher heat release rate during premixed combustion and mixing controlled combustion phase due to its higher heat release, even though nitrogen oxide (NOx) formations were increased slightly.

• Proceedings of the KSME Conference
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• 2007.05b
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• pp.3282-3287
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• 2007

Lean combustion is one of the most promising method for increasing engine efficiency and reducing the exhaust emission from SI gas engines. Due to the possibility of partial burn and misfire, however, under lean burn operation, stable flame kernel formation and fast burn rate are needed to guarantee a successful subsequent combustion. Experiment data were obtained on a single-cylinder CNG fueled SI engine to investigate the effect of direct injection, spark timing and variation of injection timing. Experimental results show that lean burn limit is ${\lambda}$=1.3 with port injection, and expansion of lean burn limit ${\lambda}$=1.4 with direct injection method, due to increase of turbulence intensity in cylinder and stratified charge. Combustion duration in lean region is improved by using the variation of injection timing.

• Journal of ILASS-Korea
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• v.17 no.2
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• pp.77-85
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• 2012

The CFD simulation of diesel spray tip penetrations were compared with 0-D simulation for experimental data obtained with common rail injection system. The simulated four injection patterns include single, pilot and split injections. The CFD simulation of the spray penetration over these injection patterns was performed using the KIVA-3V code, which was implemented with both the standard KIVA spray and original gas jet sub-models. 0-D simulation of the spray tip penetration with time-varying injection profiles was formulated based on the effective injection velocity concept as an extension of steady gas jet theory. Both the CFD simulation of the spray tip penetration with the standard KIVA spray model and 0-D simulation matched better with the experimental data than the results of the gas jet model for the entire fuel injection patterns.

• Proceedings of the KSME Conference
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• 2000.04b
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• pp.913-918
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• 2000

Spray and combustion characteristics were investigated to find suitable injection pressure by using ultra high pressure injection and single shot diesel combustion systems. As injection pressure was increased, spray penetration and spray angle were increased continuously until 2,000bar, but after this injection pressure region the rate of increase was decreased suddenly. Combustion characteristics were also enhanced until 2,000bar of injection pressure.

• 한국연소학회:학술대회논문집
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• 2012.11a
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• pp.179-182
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• 2012

Effect of double post injections on diesel PCCI combustion with focus on HC emission was investigated in a single-cylinder direct-injection diesel engine. The ISFC, HC and CO emissions were reduced by single or double post injections. The application of double post injections could also improve the trade-off relationship between NOx and HC emissions under wide EGR rate range.