• Title/Summary/Keyword: Silver-care

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Oxidative Inactivation of Peroxiredoxin Isoforms by H2O2 in Pulmonary Epithelial, Macrophage, and other Cell Lines with their Subsequent Regeneration (폐포상피세포, 대식세포를 비롯한 각종 세포주에서 H2O2에 의한 Peroxiredoxin 동위효소들의 산화에 따른 불활성화와 재생)

  • Oh, Yoon Jung;Kim, Young Sun;Choi, Young In;Shin, Seung Soo;Park, Joo Hun;Choi, Young Hwa;Park, Kwang Joo;Park, Rae Woong;Hwang, Sung Chul
    • Tuberculosis and Respiratory Diseases
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    • v.58 no.1
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    • pp.31-42
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    • 2005
  • Background : Peroxiredoxins (Prxs) are a relatively newly recognized, novel family of peroxidases that reduce $H_2O_2$ and alkylhydroperoxide into water and alcohol, respectively. There are 6 known isoforms of Prxs present in human cells. Normally, Prxs exist in a head-to-tail homodimeric state in a reduced form. However, in the presence of excess $H_2O_2$, it can be oxidized on its catalytically active cysteine site into inactive oxidized forms. This study surveyed the types of the Prx isoforms present in the pulmonary epithelial, macrophage, endothelial, and other cell lines and observed their response to oxidative stress. Methods : This study examined the effect of exogenous, excess $H_2O_2$ on the Prxs of established cell lines originating from the pulmonary epithelium, macrophages, and other cell lines, which are known to be exposed to high oxygen partial pressures or are believed to be subject to frequent oxidative stress, using non-reducing SDS polyacrylamide electrophoresis (PAGE) and 2 dimensional electrophoresis. Result : The addition of excess $H_2O_2$ to the culture media of the various cell-lines caused the immediate inactivation of Prxs, as evidenced by their inability to form dimers by a disulfide cross linkage. This was detected as a subsequent shift to its monomeric forms on the non-reducing SDS PAGE. These findings were further confirmed by 2 dimensional electrophoresis and immunoblot analysis by a shift toward a more acidic isoelectric point (pI). However, the subsequent reappearance of the dimeric Prxs with a comparable, corresponding decrease in the monomeric bands was noted on the non-reducing SDS PAGE as early as 30 minutes after the $H_2O_2$ treatment suggesting regeneration after oxidation. The regenerated dimers can again be converted to the inactivated form by a repeated $H_2O_2$ treatment, indicating that the protein is still catalytically active. The recovery of Prxs to the original dimeric state was not inhibited by a pre-treatment with cycloheximide, nor by a pretreatment with inhibitors of protein synthesis, which suggests that the reappearance of dimers occurs via a regeneration process rather than via the de novo synthesis of the active protein. Conclusion : The cells, in general, appeared to be equipped with an established system for regenerating inactivated Prxs, and this system may function as a molecular "on-off switch" in various oxidative signal transduction processes. The same mechanisms might applicable other proteins associated with signal transduction where the active catalytic site cysteines exist.

A Study on the Influence of Consumer Type on the Choice of Next-Generation Eco-Friendly Vehicle and Consumer Purchase Intention - Comparative Study on Japan and Korea - (소비자 유형이 차세대 친환경자동차선택속성과 소비자 구매의도에 미치는 영향에 관한 연구 - 한국 일본 비교연구 -)

  • Yim, Ki-Heung;Chong, Min-Young
    • Journal of Digital Convergence
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    • v.15 no.11
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    • pp.133-146
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    • 2017
  • In recent years, the development and market participation of major makers of next-generation eco-green vehicles has been accelerating. Consumer interest has also increased. Consumer characteristics, consumption type, characteristics of next-generation eco-friendly vehicles, and government policies on next eco-green vehicles. The results of this study are as follows. In Korea, there was no significant difference by gender, age, monthly average income, and consumer type. However, there was no significant difference in purchase intention by gender, age, and monthly income, Respectively. In the case of Japan, there was no significant difference by gender, age, monthly income, and consumer type. In Korea, on the other hand, images such as brand, color, and design have positive effects on eco-friendly vehicles. In the case of Japan, image and stability have a positive effect on consumers' purchasing behavior. Therefore, it is important for Japanese consumers to consider not only the image of purchasing an eco-friendly vehicles, but also the safety of the vehicles body, appear. In the case of Korea, the socio-environmental value-seeking type has a significant relationship with the purchasing intention. In the case of socio-environmental value-seeking type, the government's support policy such as carbon dioxide tax, direct support from the national or local governments, gasoline tax, Carbon tax and fuel related tax relief showed positive effects. In the case of Japan, the price-seeking type and the socio-environmental value-seeking type were found to have a significant relationship with the purchasing intention. Both the price-seeking type and the socio-environmental value-seeking type showed that the carbon dioxide tax, Direct support, gasoline tax, gasoline tax, and carbon tax, etc. have positive effects.

PROPAGATION OF GRASS CARP AND SILVIR CARP, 1971 (초어 및 백련의 종묘 생산에 관한 연구, 1971)

  • KIM In-Bae;PAIk Eui In
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.4 no.3_4
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    • pp.113-120
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    • 1971
  • In 1971, about 150,000 fingerlings of grass and silver carps were produced and distributed from the Fishculture Laboratory of Pusan fisheries College. The adults Were those transplanted from Japan in 1963. Findings during the process of this production are summarized as following : 1. Brood fish must be handled with a great care during the catching, hormone injection and maturity inspection so as the fish are not injured, otherwise, the eggs would not mature perfectly. Scale fall also affects significantly. 2. The amount of pituitary to be injected is preferable to be 2 to 3 times or more in donor's body weight to obtain good results. 3. The eggs should be spawned or stripped and inseminated as soon as mature. If the spawning is delayed the eggs become overmature and the hatching rate decreases significantly. 4. The water once used for the incubation of eggs should not be reused. The eggs under hatching process were melted away when water once used for incubation was reused. 5. A great care must be paid to keep water in quality when the net cage culture system is employed for raising fry or early fingerlings of Chinese carps. The best method to keep water in quality is to supply water in the cage continuously through a pipe or hose. 6. Heavy outbreaks of Spirogyra occured when clear well water was supplied into the cage, and a great number of fry or fingerlings were trapped or entangled resulting in a significant decrease of fish under rearing. It was prevented when moderately bloom-ed pond water was supplied into the cage. 7. Silver carp fry are usually captured and transported in the cool season because they can not be handled in the warm season owing to a high mortality, but cage cultured small size fingerlings ranging from 1.5 to 2 cm in total length are easily handled and transported even in the hot summer season, thus it enables the fish farmers to start growing them one season earlier.

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The Effect of Well-being Disposition and Appearance Concern on Cosmetic Purchase Intention and Brand Loyalty for Elderly Women (노년 여성의 웰빙 성향과 외모관심도가 화장품 구매의도 및 충성도에 미치는 영향)

  • Hong, Byung-Sook;Cho, You-Hyun;Baik, In-Sun;Lee, Eun-Jin;Park, Sung-Hee;Kwon, Yoo-Jin
    • Journal of the Korean Society of Clothing and Textiles
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    • v.30 no.12 s.159
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    • pp.1778-1787
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    • 2006
  • Appearance concern of elderly women has been rapidly increasing with the expansion in aesthetic desire for growing old gracefully and pursuing a healthy and qualitative life. In this regard, the cosmetic industry has great possibilities and it is necessary to develop actively elderly women-oriented silver marketing. The purpose of this study was to review well-being disposition and appearance concern of elderly women. Furthermore, this study investigated the influence of well-being disposition and appearance concern on cosmetic purchase intention and brand loyalty. The sample of this research consisted of 209 elderly women over 55, which was selected from Seoul and the metropolitan area. Frequency analysis, factor analysis, the analysis of confidence level and regression analysis were used for the data analysis using SPSS. The study results showed that the factor of well-being disposition for elderly woman can be classified into an environment-oriented factor, an life satisfication factor and a physical health factor. In addition, the factor of appearance concern can be classified into 3 factors: serious consideration of appearance, appearance care and skin care. Among these factors, the physical health factor of well-being disposition and the factor for serious consideration of appearance related to appearance concern had effects on cosmetic purchase intention, which fumed out to be a potent influence on brand loyalty.

Health Status and Medical Utilization of Women in Rural Area (농촌지역 여성의 건강수준과 의료이용에 대한 연구)

  • Shin, Hyung-Chul;Kang, Ji-Young;Park, Woong-Sub;Kim, Sang-A
    • Journal of agricultural medicine and community health
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    • v.34 no.1
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    • pp.67-75
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    • 2009
  • Objectives: This study was conducted to examine health inequality for gender and region in Korea. Especially it focused on health status such as disease prevalence and medical utilization of rural women. Methods: Data from the Korea national health and nutrition survey in 2001 were used. The final sample size was 37,108 individuals with age 20 and over. This study applied the logistic regression for nominal variables such as disease prevalence and unmet care needs and with the regression for continuos variables such as the length and costs of medical services. Results: Rates of disease prevalence and unmet care needs for chronic disease in rural area are higher than those in middle cities and big cities, and regional differences of those for women are more than those for mens with controlling ages. There could be interaction effect with region and sex. Conclusions: This study suggests that health policy maker should take consider of special status of rural women who are in health inequality.

A New Generational Spirit?: A Study on Welfare Attitude of Korean Young Generations (새로운 세대정신?: 한국청년세대의 복지태도 지형연구)

  • Sin-Young Kim
    • The Journal of the Convergence on Culture Technology
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    • v.9 no.6
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    • pp.17-23
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    • 2023
  • This study purports to explore the landscape of welfare attitudes of young generation of Korea in their 20s and 30s focusing upon potential differences from those of older generations. Korea has recently been in the significant debate on pension reform and the disadvantages of relatively young generations has been on of the most crucial issues during the reform. Survey data from 17th Korean Welfare Panel are analyzed and such variable as attitudes toward government expenditure on public pension, health care, old age support, poverty, family and child care and so on. In addition, welfare-related variables such as universalism vs selectivism, tax increase for welfare expenditure, and political orientation are to be analyzed. The results show several findings. First of all, correspondence analysis shows that young generation in Korea are strongly associated with higher education and full time employment compared to older generations. Secondly, the most interested welfare issues of young generations are housing and child support. Moreover, young generations' attitudes toward government expenditure increase differ from those of older generations on the issues of public pension, housing, and family and child support. Lastly, political orientation of those young generation tend to be progressive and they support universalism in welfare policy, but they do not support tax increase for welfare purpose, which, I would say, is inconsistent.

The Prevalence of Chronic Diseases, Status of Health Behaviors and Medical Service Utilization - Focused on Female Blue-Collar Workers - (노동형태에 따른 근로자의 만성질환 유병, 건강행태 및 의료이용 수준 - 여성육체근로자를 중심으로 -)

  • Kim, Sang-A;Song, In-Han;Wang, Jung-Hee;Kim, Yun-Kyung;Park, Woong-Sub
    • Journal of agricultural medicine and community health
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    • v.35 no.3
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    • pp.239-248
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    • 2010
  • Objective: Despite the increasing number of female participation in employment, blue-collar women have been exposed to higher health risk. This study is to describe the prevalence of chronic diseases, health behaviors, and medical service utilization of female blue-collar workers. Methods: Data were derived from the 2001 Korea National Health and Nutrition Survey (KNHANES). The sample was made up of 37,108 male and female participants aged 20 or over selected nation-wide by probability sampling from Korea. This study applied the logistic regression for nominal variables such as disease prevalence and health behaviors and with the regression for continuos variables such as the length and costs of medical services. Results: In general, women's prevalence of chronic illness and uncured rate were significantly higher than male, and especially female blue-collar workers had the highest prevalence, uncured rate, unhealthy status, and perceived stress. However, the medical care cost was the lowest in female blue-collar workers. Conclusions: The findings suggest that female blue-collar workers were more likely to experience health problems, and that despite the highest health risk, health service is not effectively utilized, and health policy maker should take consider of special status of female blue collar workers who are in health inequality.

The Effect of the Cognitive State of the Elderly on the Performance Level in the Activities of Daily Living(ADL) (노인의 인지상태가 일상생활활동 수행정도에 미치는 영향)

  • Kim, Soon-Ja
    • Journal of Korean Physical Therapy Science
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    • v.15 no.2
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    • pp.41-51
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    • 2008
  • Background: The purpose of this study is to determine how the cognitive state of the elderly influences the activities of daily living(ADL) in order to increase their achievement capacity and their ability to exercise this capacity in daily life. The MMSE-K(Mini-Mental State Examination-Korean) was used to evaluate the daily activities and cognitive state of a group of subjects more than 65 years of age. The goal was to understand (1) How their achievement level in their daily activities changes according to the increase and decrease of their cognition points by analyzing and comparing their achievement points in the mental state examination, and (2) What variables influence that daily achievement level. Methods: The survey was conducted by a group of students from AnSan College who were trained for this method of data collection. The group surveyed 224 senior citizens older than 65 to determine their ADL performance. The subjects were selected from Silver Care Centers in the Gyounggi Province area including centers in Suwon, Anyang, Kwachun, and Sanbon City. The subjects were given enough information to fully understand the purpose of the study and the method by which it would be conducted. The survey method was a direct interview: which involved an interviewer reading the questionnaire to the subject and recording the answer. Result: The following results were calculated using SPSS WIN ver. 13.0: In the lower score of the MMSE-K regarding ADL performance (K-MBI) for activities such as individual hygiene, bathing activity, food activity, toilet management, going up stairs, getting dressed ad undressed, controlling stools and urination, walking, and chair/bed movement, most of the subjects responded that they could not perform these activities by themselves (p<.01). In the higher scores for the same activities, most response that they could perform the tasks by themselves (p<.01). In the results of the multiple regression analysis, there influence of the variables for ADL performance (K-MBI) and MMSE-K score was higher for females than males. Conclusion: This study evaluated the cognitive state of the elderly using the MMSE-K, which is the most convenient method, and examined how the cognitive state influences the achievement capacity of the daily lives with other variables. In conclusion, the higher the score on the MMSE-K, the more independent the elders are in the activities of daily living, especially among women more than men.

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A Study on the Influence of Consumer Type on Consumer Intention to Purchase Eco-friendly Vehicles in the Service Management of Convergence Industry (융복합 산업의 서비스경영에서 소비자 유형이 소비자의 친환경자동차에 대한 구매의도에 미치는 영향에 관한 연구)

  • Yim, Ki-Heung
    • Journal of Digital Convergence
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    • v.15 no.10
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    • pp.221-232
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    • 2017
  • In recent years, the development and market participation of major makers of next-generation eco-friendly vehicles has been accelerating. Consumer interest has also increased. Consumer characteristics, consumption type, characteristics of next-generation eco-friendly vehicles, and government policies on next-the results of this study are as follows. As a result of this study, it was found that the characteristics of consumers (by sex, age, monthly average income), types of consumers (price value pursuit type, individual value pursuit type), There was no significant difference between the consumers, and the purchasing intention was not significantly different by gender, age, and monthly income, but there was a significant difference in purchase intention according to the type of consumer. The positive effects of consumer purchase behavior on brand image, color, design, etc, have positive (+) effect on eco - friendly vehicles. And social environment value seeking type among consumer types are significant in relation to purchase intention. To have three support the government's carbon dioxide (per ton of 5,000 won, per ton of 10,000 won), national or local government vehicle price support directly gasolin tax, disel tax, carbon taxes, such as fuel-related tax relief, etc. was found to have positive effect.

The Validity of Reliability of Computerized Comprehensive Neurocognitive Function Test in the Elderly (고령자 대상 전산화 종합 신경인지 검사의 타당도 및 신뢰도 분석)

  • Park, Hee Su;Yang, No Yeol;Moon, Jong Hoon;Yu, Chang Ho;Jeong, Sang Mi
    • Journal of rehabilitation welfare engineering & assistive technology
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    • v.11 no.4
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    • pp.339-348
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    • 2017
  • The purpose of this study was to verify the validity and reliability of the computerized general neurocognitive test using intuitive evaluation techniques to reflect the needs of the elderly and to validate neurocognitive function appropriately. The subjects were 100 elderly people who were over 60 healthy people. To evaluate the comprehensive neurocognitive function of the elderly, Computerized Neuro-cognitive Function Test (CNT, cybermedic. Co., Korea) developed by CyberMedic was used. The test consist of attention test, memory test and the problem solving ability test. As a result of correlation analysis of CNT test items, it was possible to confirm the characteristics of measuring single domain of attention and memory test. The problem-solving ability test also showed a high level of significant correlation, although the purpose of measurement was different, but a comprehensive cognitive function test for problem solving was possible. In the reliability analysis, the half reliability and internal consistency of test - retest were significantly higher. As a result of the above study, we conclude that the comprehensive neurocognitive test items constituted in this study have achieved reproducibility and effectiveness.