• International Journal of Industrial Entomology and Biomaterials
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• 제29권2호
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• pp.162-168
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• 2014

1-Deoxynojirimycin(1-DNJ or DNJ), a component in silkworm powder, prevents glucose from being absorbed into the bloodstream from the small intestine by inhibiting ${\alpha}$-glucosidase activity. This study compared the functional components of 1-DNJ from various silkworm species using a gene database with those of 1-DNJ produced by silkworms bred through cross-combinations. We utilized comparisons of geographical origins and species of silkworms using a gene database and discovered that 1-DNJ activity was ranked in the following order by species, Japanese (SK-1) > Chinese (C48) > European (Rock191). 1-DNJ constituted varying percentages of silkworm organs in the following order, blood > epithelial tissue > body fat > silk glands. With regard to sex, 1-DNJ, levels were higher in males than females. However, 1-DNJ levels with respect to various genetic traits (blood color, silk color, and egg color) were consistent. In order to study 1-DNJ changes that occurred during cross breeding of the silkworm gene, we bred cross-combinations utilizing SK-1 and C48 silkworms. In conclusion, in order to provide information about the constituents of functional materials contained in silkworm powder, it is imperative that silkworm cross breeding occurs so that the database of functional materials extracted from silkworms will expand.

• International Journal of Industrial Entomology and Biomaterials
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• 제13권2호
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• pp.85-95
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• 2006

Seveval Chinese and Japanese varieties with good characters were used in the breeding. After 5 years (15 generations), a pair of robust and high quality silkworm variety with NPV resistance was bred by means of a combination of crossing and pedigree selection complemented by the selection of NPV resistance. The variety was identified jointly nationwide in 2003 and 2004, and appraised by National Mulberry and Silkworm Appraising Committee. Results are as follows: its cocooning rate is over 93%, shell rate 23-25%, filament length 1200-1300 meters, reelability 75-88%, Length of non-broken cocoon filament 900-1100 meters, raw silk rate 17-19%, neatness 95-97 points, and cocoon crop, cocoon shell weight and raw silk weight per 10000 larvae is higher than those of the control variety by 7-10%, 14-19% and 14-18%, respectively. The variety is not only robust, resistant to high temperature and NPV, easy to rear, uniform in hatching, molting and maturing, but also lays more eggs, and its fecundity is high. It is suitable to rear in the Yangtze River Basin, the Yellow River basin and the Pearl River basin of China.

• Natural Product Sciences
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• 제1권1호
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• pp.66-69
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• 1995

Traditional Antheraea pernyi silkworm cocoon is tawny color. The white Antheraea pernyi silkworm cocoons studied here by IR and UV are new breeds of recent breeding. We discovered that the main body of the first grade structure of silk protein in the new white is as same as the one in the traditional tawny Antheraea pernyi silkworm cocoon, yet there are small quantities of cross-linked polymer of gentisic acid and silk protein in the white silkworm cocoons. In spite of the least surviving quantity of the polymer, the white silkworm cocoons are most white and its white color is similar to white Bombyx mori silkworm cocoon.

• International Journal of Industrial Entomology and Biomaterials
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• 제4권2호
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• pp.109-115
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• 2002

With the objective of selecting suitable breeding resource material, 10 polyvoltine and 10 bivoltine breeds were drawn from the germplasm collection of Central Sericultural Research and Training Institute, Mysore, and evaluated for 3 seasons comprising one year (6 trials). Data were collected on seven traits of economic importance such as fecundity, pupation rate, cocoon yield, cocoon weight, cocoon shell weight, cocoon shell ratio and filament length, and statistically analysed with two-way classification, Joint scoring method and evaluation index. Significant seasonal variations (P < 0.01) were observed in both polyvoltine and bivoltine breeds. Polyvoltines BL27, BL36 and BL54 and bivoltines CSR2, CSR4 and Daizo scored highest ranking values in all the three methods. Hybridization was initiated based on larval markings and cocoon shapes. Seasonal variations were discussed.

• International Journal of Industrial Entomology and Biomaterials
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• 제8권2호
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• pp.189-194
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• 2004

Amylase isozyme based three multivoltine viz., N+p, Np, N+ $p^{cho}$ and two bivoltine-D6+p, D6p syngenic lines (Syn. L) were developed from germplasm (GP) stocks Nistari (N) and D6 respectively. haemolymph isozyme pattern at pH 7.0 and 8.5 depicted a total 11 number (Am $y_{1 to 6}$ at pH 7.0 and Am $y^{l to 5}$ at pH 8.5) of native proteins (NP) of various sizes are amylase isozyme expressers. Among eleven NPs, two NPs of 770 kDa (Am $y^{6}$ at pH 7.0) and 376 kDa (Am $y^3$ at pH 8.5) are $\alpha$-amylase expressers and remaining NPs of 370, 364, 350, 329 and 274 kDa at pH 7.0 and 206, 292, 416, 725 kDa at pH 8.5 are $\beta$-amylase expressers. Accordingly, digestive juice amylase isozyme pattern at aforesaid pH also depicted a total number of 10 NPs (Am $y^{1 to 5}$) at each pH 7.0 and 8.5 are amylase expressers of which NP of 387 kDa (Am $y^4$ at pH 7.0) and 780 kDa (Am $y^{5}$ at pH 8.5) are a-amylase expresser. Remaining NPs of 338,297 ＆ 216 kDa at pH 7.0 and 370, 341, 329 ＆302 kDa at pH 8.5 are $\beta$-amylase expresser. Recurrent backcross lines (RBL) viz., N+pRBL and NpRBL were developed through introgression of high shell weight character (a multigenic trait) to be used further for congenic line (Con. L) development and to understand any association with introgressed character. Isozyme pattern in haemolymph of RBLs depicted only one $\alpha$-amylase of 770 kDa at pH 7.0 and 376 kDa at pH 8.0 with three and four respective $\beta$-amylase bands but in bivoltine lines numbers of $\beta$-amylase bands vary between 1 to 2 at aforesaid pH. Variability was also observed in digestive juice of multivolitine and its RBLs but bivoltine lines express null activity at both pH except appearance of one very week $\alpha$-amylase band D6+p at pH 8.5. Overall study suggests that not a single NP at both pH is common for expression of any band of amylase isozyme i.e., a totally different set of proteins are the amylase isozyme expresser at specific pH and no molecular factor of amylase is associated in developed RBLs which showed improvement on survival, single cocoon shell weight (SCSW) and single filament length over receptor parents.s.s.s.

• International Journal of Industrial Entomology and Biomaterials
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• 제2권1호
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• pp.65-68
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• 2001

A new silkworm variety, Chunsujam, for spring rearing season is F$_1$ hybrid between Japanese race Jam145 bred by 8459/8711 and Chinese race Jam146 by 8544/M8626. Jam145, Japanese parent of the Chunsujam, showed high GCA in pupation rate and Jam146, Chinese parent, showed high GCA in pupation rate and single cocoon weight. In the local adaptability test performed at 8 local areas in spring of 1999, Chunsujam was 5% higher in larval weight, 3% in single cocoon weight, and 4% in cocoon yield from 10,000 of the 3rd molted larvae, respectively, than the check variety Baegokjam.

• International Journal of Industrial Entomology and Biomaterials
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• 제6권2호
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• pp.107-113
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• 2003

Analysis of combining ability is a widely used biometrical tool to select promising parents and hybrids, to determine the kinds and relative magnitudes of genetic variability among hybrids as well as to forecast yield attributes in early breeding generations both in plants and animals. Various statistical approaches like Jinks and Hayman (1953), Griffing (1956), Kempthorne (1957) etc. have been extensively applied in plants. These approaches have also been tried in the mulberry silkworm, Bombyx mori L. In the present review, an attempt has been made to collect most of the studies carried out on combining ability in silkworm at one place and make it available to the scientists engaged in sericultural research.

• International Journal of Industrial Entomology and Biomaterials
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• 제4권1호
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• pp.77-81
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• 2002

A new non-cocooning silkworm variety, Hachojam, suitable for autumn rearing season is single cross Fl hybrid between Japanese race Jam 307 and Chinese race Jam 126. Jam 307, Japanese parent of the Hachojam, which is a source for non-cocooning process showed a high GCA (generation combining ability) in naked pupation rate and Jam 126, Chinese parent, showed a high GCA in pupation rate and single pupal weight. In the local adaptability test performed at 8 local areas in autumn of 1999 to 2000, the naked pupation rate and thin cocoon rate of Hachojam were 64.7% and 35.3% , respectively. The pupal weight calculated from 10,000 of the 3rd molted larvae was 24% heavier in Hachcjam than the cocoon-producing, check variety Daesungjam.

• 한국잠사곤충학회지
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• 제41권2호
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• pp.94-101
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• 1999

Twelve pure silkworm lines identified based in higher G.C.A values from a line${\times}$Tecter crossing programme, were crossed with twelve breeds showing cocoon shell ratio>22% and hybrids prepared, evaluated in a Multiple index method. Seven hydrids showing average index value>50 in all the 10 economic characters were seclcted and evaluated in the laboratory. However, two hybrids viz., A3${\times}$935E and A3${\times}$916B were finally selected based on the superiority of the breeds in one day shorter larval duration and with and with higher reeling characters compared to control KA${\times}$NB4D2. The breeding procedure involed in evolution hybrids are discussed.

• International Journal of Industrial Entomology and Biomaterials
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• 제43권2호
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• pp.99-103
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• 2021

In the present study, twelve bivoltine silkworm races were evaluated for its performance based on quantitative and qualitative traits. Seven oval and five peanut cocoon shaped races were reared in different seasons of the year. By using evaluation index method, the results showed that six races were identified as potential parental races. Among oval races, 75xin, KoC, KoZ and among peanut races An 902, 7532 and QD7 were selected as base material. The identified high yielding races will be used in various breeding programs as initial parents for the production of superior bivoltine breeds/hybrids in Vietnam