• Proceedings of the Korean Society of Applied Entomolohy Conference
• /
• 2001.11a
• /
• pp.119-119
• /
• 2001

• Proceedings of the Korean Society of Applied Entomolohy Conference
• /
• 2001.11a
• /
• pp.118-118
• /
• 2001

• Proceedings of the Korean Society of Applied Entomolohy Conference
• /
• 2001.11a
• /
• pp.120-120
• /
• 2001

• Proceedings of the Korean Society of Sericultural Science Conference
• /
• 2001.10a
• /
• pp.74-74
• /
• 2001

no Abstract, See Full Text

• Proceedings of the Korean Society of Sericultural Science Conference
• /
• 2005.04a
• /
• pp.51-51
• /
• 2005

• Proceedings of the Korean Society of Sericultural Science Conference
• /
• 2005.04a
• /
• pp.50-50
• /
• 2005

• Proceedings of the Zoological Society Korea Conference
• /
• 2000.10a
• /
• pp.267.3-268
• /
• 2000

No Abstract, See Full Text

• Proceedings of the Korean Society of Applied Entomolohy Conference
• /
• 2005.05a
• /
• pp.130-130
• /
• 2005

• Proceedings of the Korean Society of Applied Entomolohy Conference
• /
• 2005.05a
• /
• pp.131-131
• /
• 2005

• International Journal of Industrial Entomology and Biomaterials
• /
• v.10 no.1
• /
• pp.11-17
• /
• 2005

In our research to identify gene involved in the cuticle protein, we cloned a novel cuticle protein gene, ApCP15.5, from the Chinese oak silkmoth, Antheraea pernyi, larvae cDNA library. The gene encodes a 149 amino acid polypeptide with a predicted molecular mass of 15.5 kDa and a pI of 9.54. The ApCP15.5 contained a type-specific consensus sequence identifiable in other insect cuticle proteins and the deduced amino acid sequence of the ApCP15.5 cDNA is most homologous to Tenebrio molitor-C1B ($43\%$ protein sequence identity), followed by Locusta migratoria-76 ($42\%$ protein sequence identity). Northern blot and Western blot analyses revealed that the ApCP15.5 showed the epidermis-specific expression. The expression profile of ApCP15.5 indicated that the ApCP15.5 mRNA expression was detected in the early stages after larval ecdysis and larval-pupal metamorphosis, and its expression level was most significant on the first day of larval ecdysis and pupal stage. The ApCP15.5 was expressed as a 15.5 kDa polypeptide in baculovirus-infected insect cells.