• 한국동물학회:학술대회논문집
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• 한국동물학회 2000년도 한국생물과학협회 학술발표대회
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• pp.270.1-270
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• 2000

No Abstract, See Full Text

• 한국동물학회:학술대회논문집
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• 한국동물학회 2001년도 공동 춘계학술대회
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• pp.81.2-81
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• 2001

No Abstract, See Full Text

• 한국잠사곤충학회지
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• 제50권2호
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• pp.76-81
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• 2012

본 연구에서는 인체에 유해한 용매를 사용하지 않고 누에 실샘으로부터 가수분해물을 제조하는 새로운 공정을 개발하였다. 제조된 누에 실샘 가수분해물의 특성 분석을 통해 누에고치 유래 세리신 가수분해물(CSH)과 구성 성분 및 물성이 상이함을 확인하였다. 또한 1 mg/ml 수용성 누에 실샘 가수분해물(SSGH)을 첨가한 경우 10% FBS를 첨가한 대조군과 동등한 세포 증식효과가 있음을 증명하였다. 결론적으로 누에 실샘 가수분해물은 혈청을 대체할 수 있는 우수한 소재임을 확인하였다. 본 연구에서 개발한 제조방법을 통해 생산된 누에 실샘 가수분해물은 향후 화장품 및 의료용 소재로 그 응용범위를 확대할 수 있을 것으로 사료된다.

• International Journal of Industrial Entomology and Biomaterials
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• 제1권2호
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• pp.171-175
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• 2000

Determined sequences of 384 randomly selected clones in a PCR-based cDNA library of Antheraea yamamai could identify expressed sequence tags (ESTs) of highly expressed gene. One EST (fibroin) appeared 15 times, one EST (40S ribosomal protein S18) twelve times, one EST (ribosomal protein S24a) eleven times, ten times (ribosomal protein S8), nine times (60S ribosomal protein L10A), seven times (60S ribosomal protein S15A, S17, S17 and seroin), six times (ribosomal protein S8), five times (ribosomal protein S24, mariner transposase and P8 protein), four times (serpin 2), three times (heat shock protein 70 and poly A binding protein), and the remaining 6 ESTs twice (amylase, KIAA1006, elongation factor-1, transposon mag, translation initiation factor 4C, QM protein, transposase). Therefore, the 94 EST make it possible to identify 24 redundant clones that are candidates for highly expressed genes in posterior silk gland of this insect. The 24 redundant EST clones were identified in GenBank, but none of them was related to A. yamamai, suggesting that there are many unidentified genes which are highly expressed in the A. yamamai genome.

• International Journal of Industrial Entomology and Biomaterials
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• 제18권2호
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• pp.113-120
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• 2009

Embryonic lethal abnormal visual (elav) is a lethal gene in Drosophila inducing the abnormal development and function of nervous system. We cloned a Bm-elav gene by bioinformatics and biological experiment, based on sequence of ELAV protein and dbEST of Bombyx mori. The full-length of Bm-elav cDNA is 1498 bp, contains a 906 bp open read frame (ORF) encoding a precursor of 301 amino acid residues with a calculated molecular weight of 34 kDa and pI of 8.99. Bm-ELAV protein precursor contains three RNA recognition motifs (RRM) in $24{\sim}91$, $110{\sim}177$ and $222{\sim}295$ bit amino acid residues respectively, and belongs to RNA-binding protein family. Bm-ELAV shared varying positives, ranging from 56% to 60% (Identities from 41% to 45%), with RRM from other species of Xenopus tropicalis, Apis mellifera, Tribolium castaneum, Branchiostoma belcheri and Drosophila. Gene localization indicated that Bm-elav is a single-copy gene, gene mapping within 12-chromosome from 7916.68 knt to 7918.16 knt region of nscaf2993. Spatiotemporal expressions pattern analysis revealed that Bm-elav expressed higher in most tested tissues and developmental stages in whole generation, such as silk gland, fat body, midgut, hemopoietic organ and ovary, but almost no expression in terminated diapause eggs. This suggested that the expression of Bm-elav in early developmental embryonic stages might induce abnormal development like in Drosophila. Cloning of the Bm-elav gene enables us to test its potential role in controlling pests by transferring the gene into field lepidopteran insects in the future.

• 농업과학연구
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• 제43권1호
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• pp.109-115
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• 2016

Silk has had a reputation as a luxurious and sensuous fabric but it is not popular due to the expensive price and poor durability. To develop the silk materials that apply the various industries, the artificially synthesized gene can be introduced into the silkworm and expressed in the silk gland. Transgenic silkworms for the mass production of green fluorescent silks are generated using a fibroin H-chain expression system. For commercial use, safety assessment of the transgenic silkworms is essential. The purpose of this study was to examine the potential acute oral toxicity of EGFP protein expressed in genetically modified (GM) fluorescence silkworm and to obtain the approximative lethal dose in the male and female at 6-weeks ICR mice. EGFP protein was fed at a dose of 2,000 mg/kg body weight in five male or five female mice. Mortalities, clinical findings and body weight changes were monitored for 1, 3, 7, 14 days after dosing. At the end of 14 day observation period, all mice were sacrificed, and the postmortem necropsy were performed. The test group was not observed death case. Also the effect was not admitted by test substance administration in common symptoms, the body weight and postmortem. The results of single-dose oral toxicity test showed that approximative lethal dose of EGFP protein expressed in fluorescence silkworm was considered to exceed the 2,000 mg/kg body weight in both sexes.

• Applied Microscopy
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• 제18권2호
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• pp.77-90
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• 1988

The ultrastructure of the excretory duct of the large ampullate gland in the orb web spider, Nephila clavain L. Koch are studied with light and electron microscopes. The excretory ducts of the large ampullate glands connected with the large spinning tubes(spigots) on the anterior spinnerets are basically composed of three superposed types of the layers which are inner cuticles, monolayered epithelial cells and peripheral connective cells. According to the morphological characteristics of the cuticles and internal textures of the epithelial cells, the long excretory ducts are subdivided into three(distal, middle and proximal) portions. Especially, at the distal portion of the ducts near the spinning tubes, the electron lucent subcuticles which had the functions of water removal and orientation of silk fibers are well distributed, whereas at the middle and proximal portions these layers disappeared and instead of these, endocuticles are developed. The endocuticle contains two types of bands, which are electron dense and electron lucent. And along the length of the cuticular stem in the excretory duct, these two alternating bands are twisted spirally. In the cytoplasm of the columnar epithelial cells of the distal portion, rough endoplasmic reticula and Golgj complexes, related to the production of the cuticular materials are well developed. Between the adjacent epithelial cells, specialized septate junctions and desmosomes are formed along the plasma membranes. At the proximal portion of the duct, densely accumulated secretory materials appeared, and these are released to the inner canal by the apocrine secretion.

• Applied Microscopy
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• 제38권2호
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• pp.89-96
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• 2008

배회성거미류의 일종으로 주로 물가에 서식하는 닷거미과 (Pisauridae)의 황닷거미(Dolomedes sulfureus)를 실험재료로 실크 분비장치의 미세구조를 주사전자현미경(FESEM)으로 관찰하고, 토사관의 미세구조적 특성을 분석하였다. 황닷거미는 먹이를 포획하기 위한 거미줄을 만들지 않지만, 유충을 보호하기 위한 그물을 만드는 종류로 병상선(ampullate glands), 이상선(pyriform glands), 포도상선(aciniform glands) 등, 세 종류의 견사선과 이와 연결된 실젖 표면의 독특한 토사관을 가지고 있었다. 두 쌍의 대 병상선은 앞실젖을 통해, 그리고 또 다른 두 쌍(수컷의 경우 1${\sim}$2쌍)의 소병상선은 가운데실젖을 통해 분비관이 연결되어 있었다. 이상선은 앞실젖 표면의 토사관을 통해(암컷: 62${\sim}$68쌍, 수컷: 45${\sim}$50쌍) 분비되었고, 포도상선은 가운데실젖(암컷: 33${\sim}$40쌍, 수컷:18${\sim}$25쌍)과 뒷실젖(암컷: 42${\sim}$50쌍, 수컷: 24${\sim}$28쌍)의 토사관을 통해 분비됨이 확인되었다. 이들 중, 병상선과 연결된 토사관이 암수 모두에서 가장 현저한 분비 특성을 가진 것으로 관찰되었다.

• International Journal of Industrial Entomology and Biomaterials
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• 제3권1호
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• pp.69-73
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• 2001

The effect of topical application 100,300 and 500 $\mu$g/ ml with Mineral Salts of magnesium sulphate and potassium nitrate on economic parameters was ana- lyzed following treatment of last larval stadium. The treated larvae showed significantly decreased larval weight and silk gland weight in all the treated groups along with other decreased Iarval, cocoon and adult parameters. The female cocoon weight was significantly decreased in all the treated groups with decreased female cocoon shell weight and male cocoon weight at 300 and 500$\mu$g/ml respectively. The length and weight of filament was significantly decreased at 300 and 500 $\mu$g/ml respectively and denier at 500 $\mu$g/ ml. The fecundity decreased significantly in 300 and 500 $\mu$g/ml treated groups when compared with the corresponding parameters of the carrier control.

• 한국응용곤충학회:학술대회논문집
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• 한국응용곤충학회 2004년도 춘계 학술발표회
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• pp.141-141
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• 2004