• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.12
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• pp.8187-8194
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• 2015

To accelerate a heavy roller coaster train with over 1G force, a lot of thrust is required and linear synchronous motor(LSM) as propulsion method is suitable for this kind of system. To increase the propulsion efficiency of LSM, precise and real-time position information of vehicle is required for accurate phase control. However, the discontinuous position information with relatively long time interval is usually transmitted from the hall-sensors on the track every magnet length. In this paper, the basic motor model based on traditional dq-axis equations is described and the motor dynamic model is produced by considering the cogging force and friction loss. To improve the position accuracy, the position estimator is also proposed for LSM control system. Simulations were performed to check the characteristics of the torque control system which includes the position estimator based on the motor model. Simulation results based on the linearized model show that this control system has an enough bandwidth and phase margin and the executed algorithm achieves an ideal effect to follow the real-time position signal. Therefore, the feasibility of position estimator is also confirmed.

• Journal of the Korean Institute of Telematics and Electronics D
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• v.36D no.2
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• pp.20-30
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• 1999

We have made the single side hand filter for the dual channel receiver which is a heterodyne receiver to observe the cosmic radio waves with 100GHz band ranged from 85GHz to 115GHz and 150GHz band ranged from 125GHz to 175GHz simultaneously. We have introduced the filter theory using the principle of the Martion-Puplett interferometer, which has the characteristics of rotated polarization. To reduce the loss of the transmission and beam coupling which are caused from the path difference associated with the intermediate frequency the design and the implementation have been intensely considered. The receiver needs two filters with different characteristics each other. Because each of them has the optimum positions as a function frequency at which the signal frequency is fed to mixer and the image frequency is rejected to the image termination load. The intermediate frequency and its band width have been also evaluated. We have measured the property of two filters using the vector network analyser and the beam measurement system which is made by us. The responses of the filter as a function of the position and the frequency are compared with the theory. It is shown that not only the measured values are very close to the theoretical values, but also the image rejection ratios are better than 22dB for both filters. Through successful observation using a dual channel receiver with two manufactured filters, the performance of the filters has finally verified.

• Journal of Life Science
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• v.16 no.3 s.76
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• pp.485-492
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• 2006

The PrrBA two-component system is one of the major regulatory systems that control expression of photosynthesis genes in response to changes in oxygen tension in the anoxygenic photosynthetic bacterium, Rhodobacter sphaeroides. The system consists of the PrrB histidine kinase and the PrrA response regulator. The N-terminal transmembrane domain of PrrB serves as a signal-sensing domain and comprises six transmembrane helices forming three periplasmic loops and two cytoplasmic loops. The $3^{rd}$ and $4^{th}$ transmembrane helices and the $2^{nd}$ periplasmic loop were suggested to play a crucial role in redox-sensory function. In this study we demonstrated that mutations of Asp-90, Gln-93, Leu-94, Leu-98, and Asn-106 in the $2^{nd}$ periplasmic loop and its neighboring region led to severe defects in PrrB sensory function, indicating that these amino acids might be related to the redox-sensing function of PrrB. The mutant forms (D90E, D90N, and D90A) of PrrB were heterologously overexpressed in Escherichia coli, purified by means of affinity chromatography and their autokinase activities were comparatively assessed. The D90N form of PrrB was shown to possess higher autokinase activity than the wild-type form of PrrB, whereas the D90E form of PrrB displayed lower autokinase activity than the wild-type form of PrrB. The D90A mutation led to the loss of PrrB autokinase activity.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.27 no.2
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• pp.129-134
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• 2001

Since NNK is one of the most abundant tobacco-specific alkaloids and a strong carcinogenic nitrosamine, it has been used for evaluating a potential of carcinogenicity in the animal models. The present study has attempted to examine the potential of carcinogenicity of NNK in human epithelial cells, from which the cell type the most of cancers including oral cancer and nasal cavity cancer are originated. The cellular model used for the study is a human keratinocyte cell system immortalized by Ad12-SV40 hybrid virus. The cellular system has successfully been used for the carcinogenicity studies because of its limitless life span, epithelial morphology and nontumorigenicity. When cells were treated with a variety of NNK concentrations, levels of saturation density and soft agar colony formation were increased in a dose-dependent fashion. Colonies of large cell aggregates were above 5 at the higher doses. The results indicate that exposure of human cells with NNK induced loss of contact inhibition and increases of anchorage independence and cellular adhesion, which are typical characteristics of the neoplatically transformed cells. When cells were exposed with 100uM NNK for 2hr, mRNA levels of IL-1 and PAI-2 were increased in a dose-dependent manner, but expression of TGF- 1 was not affected. While expression of growth regulatory factors were altered with a short-term exposure, there was no alteration of these factors in the NNK-transformed cells. However, mRNA levels of fibronectin were increased both in the short-term treatment and in the transformation. The results suggest that altered expression of extracellular matrix such as fibronectin following short-term exposure might be fixed in the genome and these altered properties be continuously transfered throughout the cell division. Western blot analysis showed a translocation of PKC- from cytosolic fraction to the particulate fraction, indicating a possible role of NNK in the signal transduction pathway. The present study provided an evidence that NNK in the smoking may be associated with epithelial origin cancer such as oral and nasal cavity cancers. In addition, this study suggested that altered expression of extracellular matrix and PKC may play an important role in the carcinogenic mechanism of NNK.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.17 no.6 s.109
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• pp.519-528
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• 2006

This thesis is regarding of fabricating wibro PAM. First of all, we need to set specification based on link budget for wibro communication circumstance in order to develop PAM, then we decided specification concerning of wibro PAM by considering TTAS_Ko_06_0082R1 which is standarded in Korea, and IEEE Std. 802. 16d/e which is international standard. We selected the Doherty structure to increase efficiency, and pre-distorter structure to increase linearity. The fabricated PAM has not only a result of $26.5dB{\pm}1.0dB$ gain characteristics and maximum of -14 dB return loss characteristics in full frequency bands and full output ranges, but also a result of 37 dBc at 4 tone IMD characteristics which is improved result of 843 and a result of 31 dBc spurious characteristics which is improved result of 5 dB at 4.77 MHz offset point in status of having 27 % efficiency in the 26 dBm high power amplifier output signal. We confirmed the suggested structure is better than others by comparing with normal structure, balanced structure and Doherty structure without predistorter.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.18 no.4 s.119
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• pp.430-439
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• 2007

In this paper, we propose a broadband TEM horn antenna optimized using a genetic algorithm. The characteristics required for the TEM horn are the broad matching bandwidth from 2 GHz to 10 GHz and high gain in broadside with a small gain deviation within that bandwidth. In addition, a broadband balun is designed to improve the portability and to reduce the total size of the antenna. The measured return loss of the proposed TEM horn with the broadband balun is less than -10 dB(VSWR<2) from 2 GHz to 10 GHz. Compared to a conventional triangular type TEM horn, the proposed antenna shows about 80 % reduced volume and gives the broadside gain about 12 dBi with a gain deviation less than 6 dB from 2 GHz to 10 GHz. The time domain measurement shows less than 0.4 ns group delay and the pulse measurement using the transmitting signal with the rising time of 58.5 ps shows the received pulse with the rising time of 66.5 ps, which is less than 10 % rising time variation.

• BMB Reports
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• v.42 no.7
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• pp.444-449
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• 2009

Different neurodegenerative disorders like prion disease, is caused by protein misfolding conformers. Reverse-transfected cytosolic prion protein (PrP) and PrP expressed in the cytosol have been shown to be neurotoxic. To investigate the possible mechanism of neurotoxicity due to accumulation of PrP in cytosol, a PrP mutant lacking the signal and GPI (CytoPrP) was introduced into the SH-SY5Y cell. MTT and trypan blue assays indicated that the viability of cells expressing CytoPrP was remarkably reduced after treatment of MG-132. Obvious apoptosis phenomena were detected in the cells accumulated with CytoPrP, including loss of mitochondrial transmembrane potential, increase of caspase-3 activity, more annexin V/PI-double positive-stained cells and reduced Bcl-2 level. Moreover, DNA fragmentation and TUNEL assays also revealed clear evidences of late apoptosis in the cells accumulated CytoPrP. These data suggest that the accumulation of CytoPrP in cytoplasm may trigger cell apoptosis, in which mitochondrial relative apoptosis pathway seems to play critical role.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.10 no.12
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• pp.3600-3607
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• 2009

This paper describes the development of a power sensor for wireless signal over the ultra wideband range of 300~3800MHz with the detecting range of 150mW~150W. The proposed power sensor fundamentally has the function of not only detecting wireless power, but recognizing frequency and measuring VSWR. The development of the power sensor is completed through the design of dual directional coupler, design of power detector block which produces DC data using the corresponding RF input power level, and establishment of collecting the exact calibration data. The dual directional coupler has the operating frequency of 300~3800MHz with the 0.085dB of insertion loss, and directivity of 30dB at least at 3800MHz. The developed power sensor has the capability of power sensing with less than 0.25dB of resolution as well as measuring VSWR of 1.17~1.96 under the practical operating situation of very high power up to 150W at 300~3800MHz.

• Proceedings of the Korea Environmental Mutagen Society Conference
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• 2002.11a
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• pp.80-88
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• 2002

Recent studies demonstrate that collagen IV selectively pro-motes the repair of physiological processes in sublethally injured renal proximal tubular ceils (RPTC). We sought to further define the mechanisms of cell repair by measuring the effects of toxicant injury and stimulation of repair by L-ascorbic acid-2-phosphate (AscP), exogenous collagen IV, or function-stimulating integrin antibodies on the expression and subcellular localization of collagen-binding integrins (CBI) in RPTC. Expression of CBI subunits ${\alpha}_1$, ${\alpha}_2$, and ${\beta}_1$ in RPTC was not altered on day 1 after sublethal injury by S-(1,2-dichlorovinyl)-L-cysteine (DCVC). On day 6, expression of ${\alpha}_1$ and ${\beta}_1$ subunits remained unchanged, whereas a 2.2-fold increase in ${\alpha}_2$ expression was evident in injured RPTC. CBI localization in control RPTC was limited exclusively to the basal membrane. On day 1 after injury, RPTC exhibited a marked inhibition of active $Na^+$ transport and a loss of cell polarity characterized by a decrease in basal CBI localization and the appearance of CBI on the apical membrane. On day 6 after injury, RPTC still exhibited marked inhibition of active $Na^+$ transport and localization of CBI to the apical membrane. However, DCVC-injured RPTC cultured in pharmacological concentrations of AscP (500 ${\mu}$M)or exogenous collagen IV (50 ${\mu}$g/ml) exhibited an increase inactive $Na^+$ transport, relocalization of CBI to the basal membrane, and the disappearance of CBI from the apical membrane on day 6. Function-stimulating antibodies to CBI ${\beta}_1$ did not promote basal relocalization of CBI despite stimulating the repair of $Na^+$/$K^+$-ATPase activity on day 6 after injury. These data demonstrate that DCVC disrupts integrin localization and that physiological repair stimulated by AscP or collagen IV is associated with the basal relocalization of CBI in DCVC-injured RPTC. These data also suggest that CBI-mediated repair of physiological functions may occur independently of integrin relocalization.

• Applied Biological Chemistry
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• v.48 no.4
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• pp.339-344
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• 2005

Rice blast, which is caused by the fungus Magnaporthe grisea, is one of the most destructive diseases of rice. To identify genes involving in the signal transduction pathways that mediate rice blast resistance, we screened over 2,000 mutant lines of a highly resistant variety RIL260 that were generated by using a DEB (1, 3-Butadiene diepoxide) treatment method. In the mutant population, the frequency of albino plants was 6.7%, indicating that this population has a high frequency of mutations in the genome. The primary screening identified 29 mutant plants that exhibit a complete or partial loss of the resistance to rice blast. Among them, M5465, the most susceptible line, was subsequently examined by DNA gel-blot experiments using DNA molecular markers of Pi5(t) that has been previously identified as a durable resistance locus in RIL260. The result revealed that a large deletion and rearrangement of genomic DNA occurred in the Pi5(t) locus. The results suggest that DEB can be used as an efficient mutagen to induce large scale mutations in the rice genome. The isolated mutants should be useful for elucidating the Pi5(t)-mediated signaling pathways of rice blast resistance.