• Bulletin of the Korean Chemical Society
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• v.30 no.9
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• pp.1981-1984
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• 2009

Subcellular localization of protein kinase often plays an important role in determining its activity and specificity. Protein kinase C (PKC), a family of multi-gene protein kinases has long been known to be translocated to the particular cellular compartments in response to DAG or its analog phorbol esters. We used C-terminal green fluorescent protein (GFP) fusion proteins of PKC isoforms to visualize the subcellular distribution of individual PKC isoforms. Intracellular localization of PKC-GFP proteins was monitored by fluorescence microscopy after transient transfection of PKC-GFP expression vectors in the HeLa cells. In unstimulated HeLa cells, all PKC isoforms were found to be distributed throughout the cytoplasm with a few exceptions. PKC$\theta$ was mostly localized to the Golgi, and PKC$\gamma$, PKC$\delta$ and PKC$\eta$ showed cytoplasmic distribution with Golgi localization. DAG analog TPA induced translocation of PKC-GFP to the plasma membrane. PKC$\alpha$, PKC$\eta$ and PKC$\theta$ were also localized to the Golgi in response to TPA. Only PKC$\delta$ was found to be associated with the nuclear membrane after transient TPA treatment. These results suggest that specific PKC isoforms are translocated to different intracellular sites and exhibit distinct biological effects.

• The Journal of the Korea institute of electronic communication sciences
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• v.18 no.6
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• pp.1291-1298
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• 2023

Surveillance reconnaissance sensor network is used for surveillance in wartime and area of operation. In this paper, we propose a target localization method using the detection signal strength of seismic sensors. Relay equipment calculates the target location using coordinate information and detection signal strength of the seismic sensors. Target localization error deviation due to environmental factors was minimized by subtracting the dynamic offset when calculating the target location. Field test shows improvement of target localization through reduction of errors. The average error was decreased to 3.62m. Up to 62% improved result was obtained compared to weighted centroid localization method.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.62 no.2
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• pp.239-245
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• 2013

In this paper, we propose a time-frequency domain reflectometry (TFDR) based measurement method for localizing concentric neutrals corrosion on live underground power cable. It consists of two inductive couplers which can transmit the reference signal into live underground power cable and measure the reflected signals from the impedance discontinuities of concentric neutrals corrosion. In order to compensate the dispersion of the measured reflected signal via inductive coupler, an equalizer based on Wiener filtering is designed. To improve the localizing performance of concentric neutrals corrosion in the vicinity of the measurement point, the reference signal is removed from the measured reflected signals. The localization performance of the proposed method is verified by the concentric neutrals corrosion localization experiment.

• Journal of KIISE:Information Networking
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• v.37 no.6
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• pp.489-493
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• 2010

In an indoor localization method taking the lateration-based approach, the distance between a target and an AP (Anchor Point) is estimated using RSS (Received Signal Strength) measurements. Since the characteristics of a radio signal randomly vary in time and space, errors are unavoidable in distance estimation with measured RSS. Since the accuracy of distance estimation affects the localization accuracy of a lateration-based method, additional APs hearing a target have been used for localization in the literature. However, lots of experimental results show that the accuracy of a lateration-based method is improved by using carefully selected APs measuring the high quality RSSs which the distances estimated is close to the actual distances between nodes as reference APs, not using merely more APs. In this paper, we focus on selection method of reference AP and distance estimation method reflecting on environmental dynamics. We validate our method by implementing an indoor localization system and evaluating the accuracy of our method in the various experimental environments.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.12 no.8
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• pp.3657-3682
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• 2018

This paper presents a Bluetooth and pedestrian dead reckoning (PDR)-based indoor fusion localization approach (BtPDR) using smartphones. A Bluetooth and PDR-based indoor fusion localization approach can localize the initial position of a smartphone with the received signal strength (RSS) of Bluetooth. While a smartphone is moving, BtPDR can track its position by fusing the localization results of PDR and Bluetooth RSS. In addition, BtPDR can adaptively modify the parameters of PDR. The contributions of BtPDR include: a Bluetooth RSS-based Probabilistic Voting (BRPV) localization mechanism, a probabilistic voting-based Bluetooth RSS and PDR fusion method, and a heuristic search approach for reducing the complexity of BRPV. The experiment results in a real scene show that the average positioning error is < 2m, which is considered adequate for indoor location-based service applications. Moreover, compared to the traditional PDR method, BtPDR improves the location accuracy by 42.6%, on average. Compared to state-of-the-art Wireless Local Area Network (WLAN) fingerprint + PDR-based fusion indoor localization approaches, BtPDR has better positioning accuracy and does not need the same offline workload as a fingerprint algorithm.

• Journal of Institute of Control, Robotics and Systems
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• v.18 no.8
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• pp.699-705
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• 2012

In this paper, an infrastructure-based localization method using underwater wireless sensor network (UWSN) is addressed. A localization using the UWSN is necessary to widen the usage of underwater applications, however it is very difficult to establish the UWSN due to the restrictions of water. In this paper, to extend the usage of UWSN at the infrastructure, we propose a sophisticated UWSN localization method using the Received Signal Strength Indicator (RSSI) of the electromagnetic waves. During the electromagnetic waves propagating in underwater, there arises a lot of attenuation according to the distance, while the attenuation shows uniformity according to the distance. Using this characteristics, the localization system in underwater infrastructure is proposed and the experimental results show the effectiveness.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2010.10a
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• pp.543-543
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• 2010

The objective of this research is estimating the location of interested sound source by using the similarity between a beamformer output in time domain and the candidate signal. The waveform of beamformer output at the location of sound source is similar with the waveform emitted by that source. To estimate the location of sound source by using this feature, we define quantified similarity between candidate signal and beamformer output. The candidate signal describes the signal which is generated by interested source. In this paper, similarity is defined by four methods. The two methods use time vector comparison, and the other two methods use time-frequency map or linear prediction coefficients. To figure out the results and performance of localization by using similarities, we demonstrate two conditions. The one is when two pure tone sources exist and the other condition is when several bird sounds exist. As a consequence, inner product with two time-vectors and structural similarity with spectrograms can estimate the locations of interest sound source.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.13 no.5
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• pp.55-63
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• 2013

In cognitive radio networks, acquirement of position information of primary user is very important to secondary network since localization information of primary users can be utilized for improving the spectrum efficiency of secondary network and for avoiding harmful interference to primary users by using proper power control. Among various location methods, Received Signal Strength (RSS)-based localization has been widely used for distance measurements in the location detection process despite its inherent inaccuracy because it can be easily implemented without any additional hardware cost. In the RSS-based localization, the distance is measured by the received signal strength, and distance error can be caused by many factors such as fading, shadowing and obstacle between two nodes. In the paper, therefore we propose a localization scheme based on estimation of path-loss exponent to localize the location of primary users more accurately. Through simulations, it is shown that the proposed scheme can provide less localization error and interference rate to primary users than other schemes.

• Convergence Security Journal
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• v.10 no.2
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• pp.11-17
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• 2010

Recent technological advances allow us to envision a future where large numbers of low-power, inexpensive sensor devices are densely embedded in the physical environment, operating together in a wireless network. This paper considers localization for mobile sensors; localization must be invoked periodically to enable the sensors to track their location. Localizing more frequently allows the sensors to more accurately track their location in the presence of mobility. In this paper, we test and analyze the accuracy of a moving node localization by Received Signal Strength (RSS).

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.4 no.1
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• pp.45-61
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• 2010

In the communication channel, the received signal is affected by many factors that can cause errors. These effects mean that received signal strength (RSS) based methods incur more errors in measuring distance and consequently result in low precision in the location detection process. As one of the approaches to overcome these problems, we propose using direction calibration to improve the performance of the RSS-based method for distance measurement, and sequentially a weighted least squares (WLS) method using reliability factors in conjunction with a conventional RSS weighting matrix is proposed to solve an over-determined localization process. The proposed scheme focuses on the features of the RSS method to improve the performance, and these effects are proved by the simulation results.