• Journal of Biomedical Engineering Research
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• v.19 no.2
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• pp.143-152
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• 1998

In this paper, we present the performances of a Doppler system using single channel RF(Radio Frequency) sampling. This technique consists of undersampling the ultrasonic blood backscattered RF signal on a single channel. Conventional undersampling method in Doppler imaging system have to use a minimum of two identical parallel demodulation channels to reconstruct the multigate analytic Doppler signal. However, this system suffers from hardware complexity and problem of unbalance(gain and phase) between the channels. In order to reduce these problems, we have realized a multigate pulsed Doppler system using undersampling on a single channel, It requires sampling frequency at $4f_o$(where $f_o$ is the center frequency of the transducer) and 12bits A/D converter. The proposed " single-Channel RF Sampling" method aims to decrease the required sampling frequency proportionally to $4f_o$/(2k+1). To show the influence of the factor k on the measurements, we have compared the velocity profiles obtained in vitro and in vivo for different intersequence delays time (k=0 to 10). We have used a 4MHz center frequency transducer and a Phantom Doppler system with a laminar stationary flow. The axial and volumetric velocity profiles in the vessel have been computed according to factor k and have been compared. The influence of the angle between the ultrasonic beam and the flow axis direction, and the fluid viscosity on the velocity profiles obtained for different values of k factor is presented. For experiment in vivo on the carotid, we have used a data acquisition system with a sampling frequency of 20MHz and a dynamic range of 12bits. We have compared the axial velocity profiles in systole and diastole phase obtained for single channel RF sampling factor.ng factor.

• Animal cells and systems
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• v.4 no.2
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• pp.157-163
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• 2000

Yeast pheromone a-factor is a 13-amino acid peptide hormone that is synthesized as a part of a larger precursor, prepro-$\alpha$-factor, consisting of a signal peptide and a proregion of 64 amino acids. The carboxy-terminal half of the precursor contains four tandem copies of mature $\alpha$-factor. To investigate the molecular basis of intracellular sorting, proteolytic processing, and storage of the peptide hormone, yeast prepro-$\alpha$-factor precursors were heterologously expressed in rat pituitary $GH_3 cells. When cells harboring the precursor were metabolically labeled, a species of approximately 27 kD appeared inside the cells. Digestion with peptide: N-glycosidase F (PNG-F) shifted the molecular mass to a 19 kD, suggesting that the 27 kD protein was the glycosylated form as in yeast cells. The nascent polypeptide is efficiently targeted to the ER in the $GH_3 cells, where it undergoes cleavage of its signal peptide and core glycosylation to generate glycosylated pro-a-factor. To look at the post ER intracellular processing, the pulse-labelled cells were chased up to 2 hrs. The nascent propeptides disappeared from the cells at a half life of 30 min and only 10-25% of the newly synthesized, unprocessed precursors were stored intracellularly after the 2 h chase. However, about 20% of the pulse-labeled pro-$\alpha$-factor precursors were secreted into the medium in the pro-hormone form. With increasing chase time, the intracellular level of propeptide decreased, but the amount of secreted propeptide could not account for the disappearance of intracellular propeptide completely. This disappearance was insensitive to lysosomotropic agents, but was inhibited at $16^{circ}C or 20^{\circ}C$, suggesting that the turnover of the precursors was not occurring in the secretory pathway to trans Golgi network (TGN) or dependent on acidic compartments. From these results, it is concluded that a pan of these heterologous precursors may be processed at its paired dibasic sites by prohormone processing enzymes located in TGN/secretpry vesicles producing small peptides, and that the residual unprocessed precursors may be secreted into the medium rather than degraded intracellularly.

• Korean Journal of Microbiology
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• v.30 no.3
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• pp.181-186
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• 1992

Virginiae butanolide C (VB-C) binding protein binds to virginiamycin inducing factor and the protein may function as a possible pleiotropic signal transducer. To further understand signal transducing mechanism, some properties of VB-C binding protcin were investigated. VB-C binding activity was gradually increased during 60 hrs incubation: whereas the amount of produced VBs was not changed. However. VB-C hinding activity was decreased by 30-5096 in the presence of genome DNA. The binding protein could he phosphorylated by [$\gamma-^{32}\textrm{P}$] ATP. These results suggest that the DNA binding and phosphorylation may be involved in signal transducing mechanism.

• Journal of Communications and Networks
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• v.16 no.3
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• pp.280-292
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• 2014

This article presents a novel cooperative spectrum sharing (CSS) scheme. The primary transmitter transmits a complex Quadrature amplitude modulation (QAM) signal in the first phase, and CSS occurs in the second phase. The secondary transmitter with the largest forwarding channel gain among the nodes that successfully decode the primary signal in the first phase is selected for CSS. This selected node employs a pulse-amplitude modulation (PAM) signal for primary information message (IM) instead of the QAM signal, and it employs a modified PAM signal for the secondary IM. The proposed modified PAM signal depends on the amplitude of the primary PAM signal. This method results in no mutual interference and negligible primary interference constraint and allows a higher degree of exploitation of spatial diversity, thus enabling increase in secondary power to improve primary transmission. The outage performance is enhanced in both the primary and secondary systems. The critical region, in which the primary outage performance is enhanced with the proposed CSS scheme, can be adjusted and widened by varying either the modulation cooperation sharing factor or the number of secondary transmitters.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• v.2
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• pp.107-112
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• 2006

The previous acquisition method of GPS receiver for reference station adopts not only the coherent integration method but also the non-coherent integration method in order to enhance sensitivity under noisy environment. However, under noisy environment, the previous GPS signal acquisition method causes the non-coherent integration loss which is a major factor among losses that can be caused during GPS signal acquisition. The non-coherent integration loss also increases with the strength of the received noise. This paper has intention of analyzing the performance of the GPS signal acquisition method proposed to effectively enhance sensitivity of DGPS reference receiver under noisy environment. This paper presents that the proposed GPS signal acquisition method suppresses the non-coherent integration loss through post-processing simulation. Furthermore, with regard to the mean acquisition time, it is shown that the number of search cells of the proposed GPS signal acquisition method is much fewer than that of the previous GPS signal acquisition method.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.28 no.6D
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• pp.775-789
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• 2008

The Office for Government Policy Coordination announced in 2006, september that a scheme of pre-signal would show remarkable outcome to reduce traffic accidents. Therefore, the Ministry recommended applying preferentially the pre-signal scheme to enhancement projects for high accident frequency areas. In case that the suggested pre-signal was unilaterally introduced to the enhancement projects at intersections, it might rather cause a big trial and error in terms of traffic safety. Hence, on the basis of quantitative analysis, this study was to indicate a pre-signal's effectiveness to reduce the traffic accidents, to illustrate a trend of the accident type due to the pre-signal, and to introduce intersection type that could be appropriate for the pre-signal. The methodology adopted Comparison-Group Method which was developed by Hauer. Through this methodology, overall effectiveness to reduce the accidents is considered positive but individual effectiveness by intersection and by accident case was different. All cases of the accidents at small scale intersection demonstrated positive results to reduce its accidents, while in case of frontal collision and side-right angle collision out of the accident types, the installation of pre-signal rather caused a negative result increasing the accident in terms of the traffic safety. I hope that this study would be a useful reference for future development of the estimation of accident effectiveness. Thus, when the pre-signal is being installed in the big intersection, it is recommended operating the installation concerning carefully improvements about muliple aspects as traffic operation, traffic facility, human factor etc.

• BMB Reports
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• v.36 no.1
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• pp.66-77
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• 2003

Tea is one of the most popular beverages consumed in the world and has been demonstrated to have anti-cancer activity in animal models. Research findings suggest that the polyphenolic compounds, (-)-epigallocatechin-3-gallate, found primarily in green tea, and theaflavin-3,3'-digallate, a major component of black tea, are the two most effective anti-cancer factors found in tea. Several mechanisms to explain the chemopreventive effects of tea have been presented but others and we suggest that tea components target specific cell-signaling pathways responsible for regulating cellular proliferation or apoptosis. These pathways include signal transduction pathways leading to activator protein-1 (AP-1) and/or nuclear factor kappa B(NF-${\kappa}B$ ). AP-1 and NF-${\kappa}B$ are transcription factors that are known to be extremely important in tumor promoter-induced cell transformation and tumor promotion, and both are influenced differentially by the MAP kinase pathways. The purpose of this brief review is to present recent research data from other and our laboratory focusing on the tea-induced cellular signal transduction events associated with the MAP kinase, AP-1, and NF-${\kappa}B$ pathways.

• BMB Reports
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• v.49 no.3
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• pp.159-166
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• 2016

Several members of tumor necrosis factor receptor (TNFR) superfamily that these members activate caspase-8 from death-inducing signaling complex (DISC) in TNF ligand-receptor signal transduction have been identified. In the extrinsic pathway, apoptotic signal transduction is induced in death domain (DD) superfamily; it consists of a hexahelical bundle that contains 80 amino acids. The DD superfamily includes about 100 members that belong to four subfamilies: death domain (DD), caspase recruitment domain (CARD), pyrin domain (PYD), and death effector domain (DED). This superfamily contains key building blocks: with these blocks, multimeric complexes are formed through homotypic interactions. Furthermore, each DD-binding event occurs exclusively. The DD superfamily regulates the balance between death and survival of cells. In this study, the structures, functions, and unique features of DD superfamily members are compared with their complexes. By elucidating structural insights of DD superfamily members, we investigate the interaction mechanisms of DD domains; these domains are involved in TNF ligand-receptor signaling. These DD superfamily members play a pivotal role in the development of more specific treatments of cancer.

• Natural Product Sciences
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• v.21 no.3
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• pp.205-209
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• 2015

Fucoidan, a sulfated polysaccharide is found in several types of edible brown algae. It has shown numerous biological activities; however, the molecular mechanisms on the activity against atopic dermatitis have not been reported yet. We now examined the effects of fucoidan on chemokine production co-induced by TNF-α/IFN-γ, and the possible mechanisms underlying these biological effects. Our data showed that fucoidan inhibited the TNF-α/IFN-γ-induced production of thymus and activation-regulated chemokine (TARC) and macrophagederived chemokine (MDC) mRNA in human keratinocytes HaCaT cells. Also, fucoidan suppressed phosphorylation of nuclear factor kappa B (NF-κB) and activation of signal transducer and activator of transcription (STAT)1 in a dose-dependent manner. In addition, fucoidan significantly inhibited activation of extracellular-signal-regulated kinases (ERK) phosphorylation. These data indicate that fucoidan shows anti-inflammatory effects by suppressing the expression of TNF-α/IFN-γ-induced chemokines by blocking NF-κB, STAT1, and ERK1/2 activation, suggestive of as used as a therapeutic application in inflammatory skin diseases, such as atopic dermatitis.

• Journal of Applied Reliability
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• v.8 no.2
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• pp.101-111
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• 2008

Good diagnostics improves both the safety and system unavailability of digital safety systems. The measure of a diagnostic capability is called the Coverage Factor. Because the Failure Modes, Effects and Diagnostic Analysis (FMEDA) provides information on the failure rates and failure mode distributions necessary to calculate a diagnostic coverage factor for a component, the FMEDA can be used as a useful tool to calculate it. Through performing FMEDA on a digital signal processor (DSP) board used in a digital safety system, it is shown that some components of the DSP board can be replaced or improved to satisfy the required diagnostic coverage. That is, the FMEDA can serve as a useful verification tool to design a diagnostic capability for the DSP board.