• Journal of Microbiology and Biotechnology
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• 제18권3호
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• pp.427-433
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• 2008

The cephabacins produced by Lysobacter lactamgenus are ${\beta}$-lactam antibiotics composed of a cephem nucleus, an acetate residue, and an oligopeptide side chain. In order to understand the precise implication of the polyketide synthase (PKS) module in the biosynthesis of cephabacin, the genes for its core domains, ${\beta}$-ketoacyl synthase (KS), acyltransferase (AT), and acyl carrier protein (ACP), were amplified and cloned into the pET-32b(+) expression vector. The sfp gene encoding a protein that can modify apo-ACP to its active holo-form was also amplified. The recombinant KS, AT, apo-ACP, and Sfp overproduced in the form of $His_6$-tagged fusion proteins in E. coli BL21(DE3) were purified by nickel-affinity chromatography. Formation of stable peptidyl-S-KS was observed by in vitro acylation of the KS domain with the substrate [L-Ala-L-Ala-L-Ala-L-$^3H$-Arg] tetrapeptide-S-N-acetylcysteamine, which is the evidence for the selective recognition of tetrapeptide produced by nonribosomal peptide synthetase (NRPS) in the NRPS/PKS hybrid. In order to confirm whether malonyl CoA is the extender unit for acetylation of the peptidyl moiety, the AT domain, ACP domain, and Sfp protein were treated with $^{14}C$-malonyl-CoA. The results clearly show that the AT domain is able to recognize the extender unit and decarboxylatively acetylated for the elongation of the tetrapeptide. However, the transfer of the activated acetyl group to the ACP domain was not observed, probably attributed to the improper capability of Sfp to activate apo-ACP to the holo-ACP form.

• 한국공간구조학회논문집
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• 제7권3호
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• pp.153-165
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• 2007

연성 구조물은 축강성은 강하고 휨 강성이 매우 작은 재료를 주 구조재로 사용하므로 초기강성에 매우 민감한 구조체이다. 막 구조물을 설계하기 위해서는 우선 초기장력 도입으로 인한 구조물의 형상을 정확히 알아야 한다. 이를 위해서 모형을 통한 모델링이나 컴퓨터를 이용한 형상해석이 요구되며, 초기장력의 도입으로 형성되는 막 구조물의 곡면은 일반적으로 등장력 곡면이다. 따라서 연성 구조물의 해석과 설계는 기존의 강성구조물과는 다른 형상해석, 응력-변형해석, 재단도 작성 및 시공해석의 3단계 해석이 요구되어진다. 본 연구는 재단도 작성 프로그램 NASScut를 이용하여 경계면 곡률의 오차를 최소화 할 수 있는 삼각형 Re-mesh 패턴을 제안하고, 재단도를 작성한다.

• The Korean Journal of Physiology and Pharmacology
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• 제19권2호
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• pp.131-139
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• 2015

This study analyzed the differences in aerobic and anaerobic exercise ability and growth-related indicators, depending on the polymorphism of the ACE and the ACTN3 genes, to understand the genetic influence of exercise ability in the growth process of children. The subjects of the study consisted of elementary school students (n=856, age $10.32{\pm}0.07yr$). The anthropometric parameters, physical fitness and growth factors were compared among groups of the ACE I/D or the ACTN3 R577X polymorphisms. There were no significant differences between the anthropometric parameters, physical fitness and growth factors for the ACE gene ID or the ACTN3 gene R577X polymorphism. However, the DD type of ACE gene was highest in the side step test (p<0.05), and the DD type was significantly higher than the II+ID type (p<0.05) in the early bone age. The combined group of the ACE gene II+ID and the ACTN3 gene XX type significantly showed lower early bone age (p< 0.05). This study did not find any individual or compounding effects of the polymorphism in the ACE I/D or the ACTN3 R577X polymorphisms on the anthropometric parameters, physical fitness and growth factors of Korean children. However, the exercise experience and the DD type of the ACE gene may affect the early maturity of the bones.

• 한국전산유체공학회:학술대회논문집
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• 한국전산유체공학회 2009년 춘계학술대회논문집
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• pp.19-19
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• 2009

Dynamic Stall is a flow phenomenon which occurs on the retreating side of helicopter rotor blades during forward flight. It also occurs on blades of stall regulated wind turbines under yawing conditions as well as during gust loads. Time scales occurring during this process are comparable on both helicopter and wind turbine blades. Dynamic Stall limits the speed of the helicopter and its manoeuvrability and limits the amount of power production of wind turbines. Extensive numerical as well as experimental investigations have been carried out recently to get detailed insight into the very complex flow structures of the Dynamic Stall process. Numerical codes have to be based on the full equations, i.e. the Navier-Stokes equations to cover the scope of the problems involved: Time dependent flow, unsteady flow separation, vortex development and shedding, compressibility effects, turbulence, transition and 3D-effects, etc. have to be taken into account. In addition to the numerical treatment of the Dynamic Stall problem suitable wind tunnel experiments are inevitable. Comparisons of experimental data with calculated results show us the state of the art and validity of the CFD-codes and the necessity to further improve calculation procedures. In the present paper the phenomenon of Dynamic Stall will be discussed first. This discussion is followed by comparisons of some recently obtained experimental and numerical results for an oscillating helicopter airfoil under Dynamic Stall conditions. From the knowledge base of the Dynamic Stall Problems, the next step can be envisaged: to control Dynamic Stall. The present discussion will address two different Dynamic Stall control methodologies: the Nose-Droop concept and the application of Leading Edge Vortex Generators (LEVoG's) as examples of active and passive control devices. It will be shown that experimental results are available but CFD-data are only of limited comparison. A lot of future work has to be done in CFD-code development to fill this gap. Here mainly 3D-effects as well as improvements of both turbulence and transition modelling are of major concern.

• 대한통합의학회지
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• 제1권1호
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• pp.61-68
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• 2013

Purpose : The purpose of this study is to evaluate the stability and balance of the body, mobility complex exercise group and a student group for FMS tests to compare the differences between the two groups to identify its purpose. Method : The subjects of this study FMS measuring D University Physical Therapy, a student enrolled patients (7:7), and 7 patients total of 14 patients was conducted, compound exercise group weekly stretching 10 minutes, the 24 members who have run a complex exercise, 10 minutes of cool down stretching, 20-minute aerobic exercise were included in the study. Result : The results of this study, according to 1) Deep squat, Inline lunge, Trunk Stability Push-up there was significant difference(p<0.05), compound exercise group came out significantly higher. 2) Hurdle Step, Shoulder Mobility Reaching, Active Straight-leg Raise, Rotary stability in measured target these women flexibility because the test items that did not show a significant difference(p>0.05), the two groups averaged compared to the other items were higher. Complex exercise group, a statistically significant difference was overall average. Conclusion : FMS can not be resolved because of a compensatory mechanism to identify disparities and unbalanced movement patterns can help. Therefore, if the FMS and other tests conducted by splicing, will be utilized more profitably, and you will be able to suggest ways that can have a positive impact injury prevention is added to the side to expand the scope of the physical therapist.

• Biomolecules & Therapeutics
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• 제13권2호
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• pp.65-77
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• 2005

Present article reviews about clinical pharmacology of mycophenolic acid (MPA), the active form of mycophenolate mofetil (MMF), as widely used component of immunosuppressive regimens in the organ transplantation field. MMF, used alone or concomitantly with cyclosporine or tacrolimus, has approved in reducing the incidence of acute rejection and has gained widespread use in solid organ such as kidney, heart and liver transplantation. The application of MPA and development of MMF has shown a considerable impact on immunosuppressive therapy for organ transplantation as a new immunosuppressive agent with different mechanism of action from other drugs after early 1990s. In particular aspect, use of MMF, a morpholinoethyl ester of MPA, represented a significant advance in the prevention of organ allograft rejection as well as allograft and patient survival. In considering MMF clinical data, it is important to note that there is a strong correlation between high MPA area under curve(AUC) values and a low probability of acute allograft rejection. Individual trials have shown that MMF is generally well tolerated and revealed that MMF decreased the relative risk of developing chronic allograft rejection compared with azathioprine. Recent clinical investigations suggested that improved effectiveness and tolerability will results from the incorporation of MPA therapeutic drug monitoring into routine clinical practice, providing effective MMF dose individualization in renal and heart transplant patients. Therefore, MMF has a selective immunosuppressive effect with minimal toxicity and has shown to be more effective that other agents as next step of immunosuppressive agents and regimens that deliver effective graft protection and immunosuppression along with a more favorable side effect.

• 동의생리병리학회지
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• 제27권3호
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• pp.273-279
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• 2013

The objective of this study is to find out a reason why prescriptions have different effects on each patient who has same symptoms or same abdominal examinations and further more to classify the formulas more efficiently. In applying 's prescriptions, it is known that conventional ways such as treatment in accordance with symptoms or abdominal examinations have many advantages and problems reversely. To make up for the problems, additional references like strength of constitutional resistance and location of disease, degree of income and outgo are designed. And the notion in Oriental Medicine embracing aspects mentioned above corresponds to triple energizer. Triple energizer's exuberance-debilitation is able to draw an inference from some factors like density of skin interstices, elasticity of abdomen, appetite, digestive power. According to Exuberance-Debilitation of Triple Energizer, can be divided into five steps: weak(弱)-moderately weak(中弱)-neither weak nor strong(中)-moderately strong(中强)-strong(强). prescriptions would be dealt with those 5 steps, and it would be highly effective and consequently side effects could be reduced. On the basis of this classification method upon formula group, the prescriptions of can be applied more accurately by setting a direction through strength of constitutional resistance and location of disease and combining with existing references like symptoms, palpation and abdominal examinations.

• 한국재료학회지
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• 제25권9호
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• pp.462-467
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• 2015

Pellicle is defined as a thin transparent film stretched over an aluminum (Al) frame that is glued on one side of a photomask. As semiconductor devices are pursuing higher levels of integration and higher resolution patterns, the cleaning of the Al flame surface is becoming a critical step because the contaminants on the Al flame can cause lithography exposure defects on the wafers. In order to remove these contaminants from the Al frame, a highly concentrated nitric acid ($HNO_3$) solution is used. However, it is difficult to fully remove them, which results in an increase in the Al surface roughness. In this paper, the pellicle frame cleaning is investigated using various cleaning solutions. When the mixture of sulfuric acid ($H_2SO_4$), hydrofluoric acid (HF), hydrogen peroxide ($H_2O_2$), and deionized water with ultrasonic is used, a high cleaning efficiency is achieved without $HNO_3$. Thus, this cleaning process is suitable for Al frame cleaning and it can also reduce the use of chemicals.

• Restorative Dentistry and Endodontics
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• 제16권2호
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• pp.143-154
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• 1991

The purpose of this study was to evaluate the sealing ability of five obturation methods in conjunction with sealer. Fifty extracted upper and lower anterior teeth were selected and the access cavities were prepared lingually with a round bur. The working length was determined with a #15K file, and the root canals were instrumented with a #40K file 1mm short of the apical foramen. The apical third of root canal was flared by step-back technique and the coronal two thirds of root canal using #2 - 3 Gates Glidden drills. And then, the teeth were randomly assigned to five groups of 10 teeth each. A thin coat of Tubliseal was placed into the canal into the canal using the # 35 reamer and the canals were filled by lateral condensation, vertical condensation, ultrasonic activated lateral condensation, ULTRAFIL injecting, and McSpadden compaction methods. All teeth were stored in 100 % relative humidity at 31c for 48 hours. The roots were suspended in 2 % methylene blue solution for 48 hours, left to dry for 24 hours and then, half side of root was removed longitudinally using the fissure bur. A Boley gauge wes used to measure the distance, to the nearest 0.1mm, from the apical foramen to the most coronal level of dye penetration. The measurement of dye penetration was statistically compared by Duncan's multiple range test The results were as follows 1. Ultrasonic group showed the best sealing ability among the all experimental groups, but there were statistically no significant difference in the sealing ability between ultrasonic group and vertical condensation group. 2. There were no statistically significant difference in the sealing ability among the lateral, vertical, and ultrasonic groups. 3. McSpadden group showed the worst sealing ability among the all exprimental groups.

• Journal of Biosystems Engineering
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• 제35권1호
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• pp.37-45
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• 2010

Since SRC (Short-term Rotation Coppice) such as poplar and willow can be harvested in three years, they are known to be a potential forest biomass as fuel for a power plant. The production system including transplanting and harvesting is, however, necessary to be mechanized because such a biomass should be handled in a massive volumetric size. A pull-type stem-cutting transplanter was developed in the research as the first step to realize the production of SRC. A needle-like transplanting device pushes a stem-cutting into the prepared soil bed by a pneumatic cylinder, and another device firms soil around a stem-cutting transplanted. Since this is an intermittent operation, it was necessary to develop a zero horizontal velocity mechanism which enabled only the transplanting needle part to continue a zero horizontal movement relative to the ground during the transplanting operation even when the tractor kept moving forward. The 2-row transplanter can transplant stem-cuttings at the rate of 6.5 seconds per row without missing a single attempt. The planting depth and distance were well maintained and controlled. Their CVs were between 2.1~3.4% and 0.87~1.7% for the depth and the distance, respectively. Although, the transplanted stem-cuttings tended to lean outward from the back-view and forward from the side view, they were planted within the range of $3^{\circ}$ from the upright position.