• Fashion & Textile Research Journal
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• v.15 no.6
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• pp.1010-1017
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• 2013

Current global textiles and fashion industries have gradually shifted focus to high value-added, high sensibility, and multi-functional products based on new human-friendliness and sustainable growth technologies. Textile design CAD systems have been developed in conjunction with computer hardware and software sector advances. This study compares the patterns or images of actual woven fabrics and virtual fabrics prepared with a textile design CAD system. In this study, several weave structures (such as fancy yarn weave and patterns) were prepared with a shuttle loom. The woven textile images were taken using a CCD camera. The same weave structure data and yarn data were fed into a textile design CAD system in order to simulate fabric images as similarly as possible. Similarity Index analysis methods allowed for an analysis of the index between the actual fabric specimen and the simulated image of the corresponding fabric. The results showed that repeated small pattern weaves provide superior similarity index values than those of a fancy yarn weave that indicate some irregularities due to fancy yarn attributes. A Complex Wavelet Structural Similarity(CW-SSIM) index resulted in a better index than other methods such as Multi-Scale(MS) SSIM, and Feature Similarity(FS) SSIM, across fabric specimen images. A correlation analysis of the similarity index based on an image analysis and a similarity evaluation by panel members was also implemented.

• Journal of Microbiology and Biotechnology
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• v.13 no.6
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• pp.892-896
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• 2003

High-level expression of the lipase B26 gene from Bacillus pumilus was achieved using Bacillus subtilis Chungkookjang isolated from the Korean traditional fermented bean paste, Chungkookjang. For the secretory production of recombinant lipase B26 in a Bacillus host system, pLipB26 was constructed by ligating the lipase B26 gene into the recently designed Escherichia coli-Bacillus shuttle vector, pLipSM, and that was then transformed into B. subtilis Chungkookjang. Among the various vector, medium, and host combinations, B. subtilis Chungkookjang harboring the pLipB26 exhibited the highest lipase activity in PY medium, and B. subtilis Chungkookjang secreted two times more enzymes than B. subtilis DB 104 under the same condition. When B. subtilis Chungkookjang harboring the pLipB26 was cultured in a 5-1 jar-fermentor containing 21 of a PY medium, the maximum lipase activity (140 U/ml) and production yield (0.68 g/l) were obtained during the late exponential phase from a cell-free culture broth. Although B. subtilis Chungkookjang also secreted extracellular proteases at the late exponential phase, these results suggested the potential of B. subtilis Chungkookjang as a host for the secretory production of foreign proteins.

• Microbiology and Biotechnology Letters
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• v.14 no.2
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• pp.155-160
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• 1986

$\alpha$-Amylase gene of B. amyloliquefaciens was cloned to E. coli-yeast shuttle vector YEp-13 and expressed in E. coli. Chromosomal DNA of B. amyloliquefaciens was partially digested with Sau3Al and YEp13 plasmid was cleaved with BamH1. The hybrid plasmid, pHA28, was constructed by shotgun method and transformed to E. coli C600 and HB101. The amount of $\alpha$-amylase produced by transformants of E. coli was about 20% to 30% of that produced by B. amyloli-quefaciens. About 65% of $\alpha$-amylase produced by transformant was secreted into periplasm and the others were located in cytoplasm. $\alpha$-Amylase production was maximal when transformants were cultivated for 15hr to 20hr. As the result of agarose gel electrophoresis, pHA28 plasmid was found to be various in its size. This result suggested that pHA28 plasmid was segregated.

• Journal of Biosystems Engineering
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• v.35 no.4
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• pp.224-230
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• 2010

The purpose of this study was to develop position control system of an electric actuator for automatic shuttle shifting of a tractor. The electric actuator was installed at the link of the forward-reverse gearshift of the tractor transmission, and controlled in the ranges of forward, neutral, and reverse positions. The position control system of the electric actuator was developed based on PID (Proportional Integral Derivative) controller and transfer function of the electric actuator. The coefficients of the PID controller were determined by Ziegler-Nichols (Z-N) method and optimized using simulation program. The prototype AMT (Automated Manual Transmission) test unit of the tractor was installed and used to evaluate the performance of the position control. The evaluation system for the control performance consisted of forward-reverse actuator, motor driver, and controller. The tests were conducted as the controlled positions of the actuator were changed from neutral position to forward, neutral, and reverse positions in sequence. The sequential tests were repeated 20 times. The operations of changing the gearshift were considered as the step response of the control system. Maximum overshoot, settling time, and steady-state error were analyzed. The results showed that performance of the position control system was reasonable and qualified. The maximum overshoots, the steady-state errors, and the settling times of the position control system were 10~20%, 1~5%, and 0.92~1.49 sec, respectively. The modifications of the electric actuator will be required to enhance the performance of position control during field operation.

• Microbiology and Biotechnology Letters
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• v.29 no.1
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• pp.1-11
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• 2001

Lactic acid bacteria (LAB) are widely used for various food fermentation. With the recent advances in modern biotechnology, a variety of bio-products with the high economic values have been produced using microorganisms. For molecular cloning and expression studies on the gene of interest, E. coli has been widely used mainly because vector systems are fully developed. Most plasmid vectors currently used for E, coli carry antibiotic-resistant markers. As it is generally believed that the antibiotic resistance markers are potentially transferred to other bacteria, application of the plasmid vectors carrying antibiotic resistance genes as selection markers should be avoided, especially for human consump-tion. By contrast, as LAB have some desirable traits such that the they are GRAS(generally recognized as safe), able to secrete gene products out of cell, and their low protease activities, they are regarded as an ideal organism for the genetic manipulation, including cloning and expression of homologous and heterologous genes. However, the vec-tor systems established for LAB are stil insufficient to over-produce gene products, stably, limiting the use of these organisms for industrial applications. For a past decade, the two popular plasmid vectors, pAM$\beta$1 of Streptococcus faecalis and pGK12 theB. subtilis-E. coli shuttle vector derived from pWV01 of Lactococcus lactis ssp. cremoris wg 2, were most widely used to construct efficient chimeric vectors to be stably maintained in many industrial strains of LAB. Currently, non-antibiotic markers such as nisin resistance($Nis^{r}$ ) are explored for selecting recombi-nant clone. In addition, a gene encoding S-layer protein, slp/A, on bacterial cell wall was successfully recombined with the proper LAB vectors LAB vectors for excretion of the heterologous gene product from LAB Many food-grade host vec-tor systems were successfully developed, which allowed stable integration of multiple plasmid copies in the vec-mosome of LAB. More recently, an integration vector system based on the site-specific integration apparatus of temperate lactococcal bacteriophage, containing the integrase gene(int) and phage attachment site(attP), was pub-lished. In conclusion, when various vector system, which are maintain stably and expressed strongly in LAB, are developed, lost of such food products as enzymes, pharmaceuticals, bioactive food ingredients for human consump-tion would be produced at a full scale in LAB.

• Microbiology and Biotechnology Letters
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• v.32 no.1
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• pp.60-66
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• 2004

The cycloinulooligosaccharide fructanotransferase(CFTase) gene (cft) from Paenibacillus polymyxa was subcloned into the E. coli-yeast shuttle vector, pYES2.0 (GALI promoter). The constructed plasmid, pYGCFT (9.9 kb) was introduced into S. cerevisiae SEY2102 cell and then the yeast transformant was selected on the synthetic defined media lacking uracil Based on the cyclofructan(CF) spots on thin-layer chromatogram, the gene under the control of GALI promoter was successfully expressed in the yeast transformant. The recombinant CFTase was not secreted into the medium and was predominantly localized in the periplasmic space. CF was started to be produced after 3h of enzymatic reaction with inulin. The pH and temperature optimum for the CF production from inulin was pH 8.0 and 45$^{\circ}C$, respectively. Enzyme activity was stably maintained up to the pH of 10.0. The examination of the inulin sources revealed that a dahlia tuber and Jerusalem artichoke were the best for the production of CF.

• Korean Journal of Microbiology
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• v.23 no.1
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• pp.56-63
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• 1985

We have characterized the plasmids of zymomonas, and constructed E. coli-Zymomonas shuttle vector. Plasmids have been detected in four strains of Zymomonas mobilis. All strains tested had at least one plasmid ranging in size from about 1.7 to 46kb. Antibiotics resistances of Z. mobilis were tested to select the host strain. All strains were very sensitive to tetracycline and chloramphenicol. Homology tests between the plasmids in four strains showed that the plasmids of ATCC10988 is highly homologous to those of ZM1, and that there is no homology between plasmids of ZM4 and Agll. The 1.7kb plasmid of ATCC10988, named as pZM886, also has no homology with plasmids of ZM4. A hybrid plasmid, designated to pBZ41, was constructed from pZM886 and pBR322. A restriction map of pBZ41 was established. Replicon of pZM886 didn't operate in E.coli and pBR322 seemed not to replicate in Zymomonas. pBZ41 was transfered from E. coli to Zymomonas by conjugal mobilization. The transconjugants were resistant to tetracycline and maintained pBZ41 stably.

• Journal of Environmental Science International
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• v.14 no.5
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• pp.457-461
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• 2005

The survey of user's satisfactory degree of location, plantings, facilities, operation, and management factors on national parks' camp sites was carried out. Many problems were analyzed and identified. This survey was for proposing the correct way to plan and draft for camping site after this. In addition to, the realization of environmental education through nature friendly recreation was aimed at. According to the result of the survey, the followings were suggested as necessities to improve the camp site. 1. Repair and replacement of insufficiently managed facilities should be aimed at. 2. Facilities for a handicapped person should be preferentially introduced. 3. Active management and publicity in low-demand season that practiced by developed countries should be carried out. Seasonal program for various age groups should be operated. 4. Surrounding tourist site, camp site, and trail should be organically connected by shuttle bus and other transports. A survey was carried out for the user satisfaction in the campsites of national parks. Based on this survey concerned with the location, planting, facilities, operation and management, the problems were identified and analysed to improve future design and plan of the campsites. This study aims for the realization of environmental education through nature friendly recreation. The following points are suggested as needed according to the survey result. 1. damaged facilities should be repaired or replaced. 2. introduction of the facilities for disabled should be prioritized. 3. consistent and active management and publicity work should be operated in the off-peak season. and the seasonal activity programs for the various age groups should be provided. 4. the site should be well connected with other surrounding campsites and tourist sites using the shuttle bus and other transport method.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.39 no.6
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• pp.576-584
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• 2011

In order to develop a launch vehicle successfully, it is important to estimate development costs accurately but it is also important to plan the annual budget. In this paper, the statistical method was utilized for cost spreading. For cost spread modeling, the suitability of the model by analyzing several statistical models was evaluated and consequently, the beta-distribution model has been selected. In this study, the validity of the annual estimation cost model was verified through the comparison of the actual development cost distribution and the estimating cost distribution of Space Shuttle Main Engine. In addition, this paper estimated the annual budget required for the development of the KSLV-II using currently allocated cost for successful development. It is anticipated that the present cost spread model can be applied to not only launch vehicle development but also other large complex system development.

• Journal of the Korean Geographical Society
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• v.45 no.5
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• pp.628-646
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• 2010

The subject of this research is the small-scale trade between South Korea and China (this trade is a so-called shuttle trade.) This research attempts to find the background to the emergence of the Sino-South Korean (cross-border) small-scale trade and the role of travel routes between the two countries. This research also aims to identify the development process of the bilateral small-scale trade by studying the scale of the small-scale trade and the organization of small-scale traders. Moreover, this research tries to discover the spatial characteristics of the interregional small-scale trade by reviewing the characteristics of traded goods; process of export and import and nodes of small-scale trade. To accomplish aforementioned purposes, the author discussed the relations between small-scale traders and maritime companies. The author also studied the internal change in the small-scale trade by focusing on the reinforcement of the regulation against the small-scale trade. Lastly, the author cited the case of the Soviet Union and middle-eastern Europe, which tremendously expanded the small-scale trades in the 1980s, in order to explain the growth of the Sino-South Korean small-scale trade.