• Cross-Cultural Studies
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• v.23
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• pp.7-32
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• 2011

In this article, we tried to introduce a little-known work of M.A. Bulgakov "Snowstorm" and provide some various clues for reading this short story. This study is focused on "snowstorm-plot", grasping the structure and the theme of the present work. To this end, in the Chapter II we tired to seek for the meanings of Bulgakov's "snowstorm" based on intertextuality shown from the works by those writers such as Pushkin, Gogol and Tolstoy. In this short story "snowstorm" is presented not only as a natural phenomenon but as a "participant" which provides young doctor-narrator with short time break and let him go to a dying bride, and at last place him on the crossroads of life and death. Indeed "snowstorm" plays a role of the framed structure of Bulgakov's text. In the Chapter III we observed the creative expression of Bulgakov's work which is comprised of overlappings with dream and reality. In other words, in the short story "Snowstorm" the outside and the inside story of frame are described as a dream of the one same night. We can guess that the Shermetievo story is a kind of dream of young doctor who fell asleep in Tuesday night, asking himself "how many patients will come tomorrow?". By the way the Shermetievo story unfolds as an incident which is happened on Wednesday. In this way in Bulgakov's "Snowstorm" it is hard to draw clear lines of demarcation between dream and reality. Therefore existential themes like these "Life and Death", "Professional calling and personal conscience", "The great nature and the week human being" are resonated with original structure "Dream in Dream".

• Journal of Microbiology and Biotechnology
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• v.33 no.12
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• pp.1681-1691
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• 2023

Flavin mononucleotide-binding proteins or domains emit cyan-green fluorescence under aerobic and anaerobic conditions, but relatively low fluorescence and less thermostability limit their application as reporters. In this work, we incorporated the codon-optimized fluorescent protein from Chlamydomonas reinhardtii with two different linkers independently into the redox-responsive split intein construct, overexpressed the precursors in hyperoxic Escherichia coli SHuffle T7 strain, and cyclized the target proteins in vitro in the presence of the reducing agent. Compared with the purified linear protein, the cyclic protein with the short linker displayed enhanced fluorescence. In contrast, cyclized protein with incorporation of the long linker including the myc-tag and human rhinovirus 3C protease cleavable sequence emitted slightly increased fluorescence compared with the protein linearized with the protease cleavage. The cyclic protein with the short linker also exhibited increased thermal stability and exopeptidase resistance. Moreover, induction of the target proteins in an oxygen-deficient culture rendered fluorescent E. coli BL21 (DE3) cells brighter than those overexpressing the linear construct. Thus, the cyclic reporter can hopefully be used in certain thermophilic anaerobes.

• The Korean Journal of Malacology
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• v.32 no.2
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• pp.133-139
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• 2016

The incubation time required for hatching of O. ocellatus were investigated through the processes of egg and embryonic developments by the dissecting microscopic and visual observations. And differences in ecological characteristics of the plankton mode of life or the benthic mode of life according to total numbers of the suckers on each short arm of the hatched juvenile larvae of O. ocellatus were studied by comparisons with other octopodidae species. Compared with the recent a few results reported by other researchers associated with the incubation time required for hatching by female adult mother of O. minor (73-90 days after spawning at $20.9-21.5^{\circ}C$ ranges), in this study, the incubation time required for hatching by female adult mother of O. ocellatus was 56-57 days after spawning at $11.0-20.4^{\circ}C$. Therefore, the incubation time required for hatching by female adult mother varied with Octopodidae species. In this studies, each ovarian egg laid by a female was connected to an egg string attaching to the surface of the wall or bottom of vacunt shell of Rapana venosa. Egg and embryonic developments of this species were studied in the indoor aquaria, in the specific gravity ranging 1.024-1.025. the hatched juvenile of O. ocellatus is 10.3 mm in the mean total length and 4.5 mm in mantle length, and each of its short arms has 18-20 suckers. The just hatched juvenile larvae of O. ocellatus enter the benthic mode of life (benthic larval stage) after hatching. In particular, regarding differences in ecological characteristics of the mode of life according to total numbers of the suckers, O. vulgaris may not need to have many suckers because they enter the planktonic mode of life after hatching, however O. ocellatus may need to have many suckers, because they should adapt to the benthic mode of life. And also the just hatched juvenile larvae of O. minor (bearing many suckers more than O. ocellatus) enter the benthic mode of life (benthic larval stage) after hatching. Therefore, the total number of the suckers on each short arm of the hatched juvenile larvae can be used for determining whether an octopus species has planktonic larval stages or benthic larval stage (benthic mode of life). In particular, The intracohort cannibalism phenomena appeared at the hatched juvenile larval stage because the larval stage of O. ocellatus and O. minor enter into the benthic larval stage in the early stage, unlike entering into the plaktonic larval stage in other Octopus species such as O. vulgaris: at this time, the early hatched larvae fed the late hatched larvae (they are the same species and almost same ages). Therefore, the intracohort cannibalism pheneomena occur in the just hatched juvenile stage of only O. ocellatus and O. minor.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.14 no.24
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• pp.105-109
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• 1991

In the comparative study of investment alternatives with different life times. It is wrong to compare a long-life time alternative with different life times. It is to wrong to compare a long-life time alternative with a short-life time one by the same measure; therefore, a measure is needed which is suitable for the characters of alternatives. The purpose of this study is in present a method to evaluate investment alternatives with different life times and to explain the assumptions and calculating method.

• ALGAE
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• v.21 no.1
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• pp.103-107
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• 2006

Rhodochorton purpureum 4187 from Jeju Island, Korea may have a sexual life history similar to that seen by other investigators working on other strains around the world. In culture short days (8:16, 11:13, 12:12 LD) at 10-15°C induced tetrasporogenesis. Discharged spores were observed with time lapse videomicroscopy. They showed a slight amoeboid movement for 2-3 minutes before rounding up and settling. Tetrasporelings develop into male and female gametophytes. No fertilisation was observed. Tetrasporangia often were borne on carpogonial clusters of females but no discharged spores were seen. Isolate 4241 from San Juan I., Washington, USA grew well in most conditions tested but did not reproduce in short days (8:16, 11:13, 12:12 LD) at 10-15°C.

• Journal of Information Technology Applications and Management
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• v.29 no.5
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• pp.27-37
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• 2022

This study intends to link agricultural machine history data with related organizations or collect them through IoT sensors, receive input from agricultural machine users and managers, and analyze them through AI algorithms. Through this, the goal is to track and manage the history data throughout all stages of production, purchase, operation, and disposal of agricultural machinery. First, LSTM (Long Short-Term Memory) is used to estimate oil consumption and recommend maintenance from historical data of agricultural machines such as tractors and combines, and C-LSTM (Convolution Long Short-Term Memory) is used to diagnose and determine failures. Memory) to build a deep learning algorithm. Second, in order to collect historical data of agricultural machinery, IoT sensors including GPS module, gyro sensor, acceleration sensor, and temperature and humidity sensor are attached to agricultural machinery to automatically collect data. Third, event-type data such as agricultural machine production, purchase, and disposal are automatically collected from related organizations to design an interface that can integrate the entire life cycle history data and collect data through this.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.37 no.2 s.110
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• pp.78-86
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• 2005

Hwaseonji(Korean traditional paper) used for writing and painting has been made from the mulberry bast-fiber and the short-length fiber pulps, wood pulps. However, besides wood pulps, other short-length fiber pulps also can be used instead of wood pulps. Hence, this research was carried out to make the various Hwaseonjis with the different properties, using the five kinds of short-length fiber pulps respectively. The short-length fiber pulps used in this research were softwood bleached kraft pulp(SwBKP) hardwood bleached kraft pulp(HwBKP), rice-straw bleached sulfite pulp(RsBSP), bamboo bleached kraft pulp(BbBKP) and recycled pulp from vellem paper(RP). And, the mixture ratios of the mulberry bast-fiber pulp and short-length fiber pulps were 100:0, 80:20, 60:40, 40:60 and 20:80. After various Hwaseonjis were made from different mixtures mentioned above with hand-made method, physical properties and chinese ink blot property of each paper were measured. The strengthes were the highest in the Hwaseonji made from the mixture of the mulberry bast-fiber pulp and SwBKP. However, chinese ink blot property and smoothness were better when RsBSP, BbBKP or RP were mixed into the mulberry bast-fiber pulp. As a result, the various kinds of Hwaseonjis which the users can choose based on their needs were made.

• International Journal of Naval Architecture and Ocean Engineering
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• v.6 no.2
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• pp.431-441
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• 2014

The effects of hull-girder vibration on the fatigue strength of a Post-Panamax container ship are discussed in the present paper. Firstly, the short-term sea states are categorized according to the occurrence probability of each sea state. Time histories of hull-girder stress in short-term sea states are calculated by means of a nonlinear simulation code of ship response assuming that the hull-girder is rigid and flexible. Then, the calculated stress peaks are processed by the rainflow counting method, where two different counting procedures are used based on the considerations of crack propagation behaviors. Finally, the fatigue damage in life time of the ship in each categorized short-term sea state is estimated by means of Miner's rule. Based on the calculated results, the effects of hull-girder vibrations on the fatigue damage are clarified by disaggregated damage from short-term sea state.

• Journal of Korean Academy of Nursing
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• v.36 no.5
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• pp.813-820
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• 2006

Purpose: This study examined the effects of individual reminiscence therapy on older adults' depression, morale, and the quality of life. Methods: The design was a single-group pre-test and post-test study. Subjects consisted of 31 older adults from two senior centers and a welfare center in Seoul. Individual reminiscence therapy was applied to study subjects four times, once a week for an hour at each time. Measurement tools were the Geriatric Depression Scale Short Form Korea (GDSSF-K) for depression, Mun Ae-ri's (1996) scale for morale, and Medical Outcomes Study Short Form 36 (SF-36) for the quality of life. Data was analyzed using descriptive statistics, paired t-test, and pearson correlation. Results: The application of individual reminiscence therapy reduced older adults' depression (t=-5.65, p=.000), and enhanced older adults' morale (t=4.65, p=.000). The application of individual reminiscence therapy improved older adults' quality of life (t=5.00, p=.000). Conclusion: Findings of the study suggest that individual reminiscence therapy may be applied as a nursing intervention that contributesto the improvement of older adults' quality of life, reduces their depression, and enhances their morale.

• BMB Reports
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• v.41 no.4
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• pp.294-299
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• 2008

Elevated expression of SMYD3 is a frequent genetic abnormality in several malignancies. Few studies knocking down SMYD3 expression in cervical carcinoma cells have been performed to date. In this paper, we established an inducible short hairpin RNA expression system to examine its role in maintaining the malignant phenotype of HeLa cells. After being induced by doxycycline, SMYD3 mRNA and protein expression were both reduced, and significant reductions in cell proliferation, colony formation and migration/invasion activity were observed in the SMYD3-silenced HeLa cells. The percentage of cells in sub-G1 was elevated and DNA ladder formation could be detected, indicating potent induction of apoptosis by SMYD3 knockdown. These findings imply that SMYD3 plays crucial roles in HeLa cell proliferation and migration/invasion, and that it may be a useful therapeutic target in human cervical carcinomas.