• FLOWER RESEARCH JOURNAL
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• v.17 no.4
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• pp.231-236
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• 2009

This study was conducted to identity the effect of plant growth retardants such as paclobutrazol, ethephon, uniconazole and daminozide by drenching on growth of Datura suaveolens 'Orange Star'. Datura suaveolens 'Orange Star' treated with the growth retardants except ethephon was gradually shortened in proportion to the treated concentration of growth retardants. Especially, uniconazole treatment dramatically short ed stem height and width of plant, diminished leaf width and length and also shortened the days to flowering, and increased flowering times per year. On the contrary, ethephon treatment showed the opposite affect such as higher plant width and plant height, increased days to flowering, and decreased the number of flower times. Dwarfing by plant growth retardants in Datura suaveolens 'Orange Star' was effective in orders of uniconazole, daminozide and paclobutrazol.

• FLOWER RESEARCH JOURNAL
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• v.16 no.3
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• pp.200-204
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• 2008

A new spary chrysanthemum(Dendranthema grandiflorum) cultivar 'Serapina' was developed from a cross between 'Relanse' and 'Yen' by selection of seedlings and lines at the Flower Research Institute, Gyeongnam Agricultural Research and Extention Services(ARES) from 2002 to 2006. Its characteristics were investigated three times from 2004 to 2006 under condition of forcing culture in spring and retarding culture in autumn. The natural flowering time of 'Serapina' was October 23th, and year-round production was possible by day length treatment. Its capitulum was 2.8cm in diameter, and had 43.4 ray florets and 28.9 head per stem in autumn. Its ray floret was yellow with green central zone. To flower in the short day condition, for 'Serapina' was about 46 days in spring, and 'Serapina' showed the vase life of 21.7 days in autumn. This cultivar was registered for a commercialization in 2007.

• Korean Journal of Breeding Science
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• v.41 no.4
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• pp.529-533
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• 2009

A new spray chrysanthemum (Dendranthema grandiflorum) cultivar "Green Witch" was developed from a cross between 'S04161' and 'S04109' by selection of seedlings and lines at the Flower Research Institute, Gyeongnam Agricultural Research and Extension Services (ARES) from 2004 to 2008. Its characteristics were investigated three times from 2006 to 2008 under condition of forcing culture in spring and retarding culture in autumn. The natural flowering time of "Green Witch" was October 19th, and year-round production was possible by day length treatment. Its capitulum was 3.2cm in diameter, and had 15.9 head per stem in autumn. Its ray floret was green central zone. To flower in the short day condition, for "Green Witch" was about 44 days in spring, and "Green Witch" showed the vase life of 25.3 days in autumn. This cultivar was registered for a commercialization in 2008.

• Korean Journal of Breeding Science
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• v.40 no.4
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• pp.490-494
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• 2008

A new spary chrysanthemum (Dendranthema grandiflorum) cultivar 'Eye Ball' was developed from a cross between 'Rima' and 'Yen' followed by selections of seedlings and lines at the Flower Rearch Institute, Gyeongnam Agricultural Research and Extension Services (ARES) from 2002 to 2006. Its characteristics were investigated three times from 2004 to 2006 under forcing culture in spring and retarding culture in autumn. The natural flowering time of 'Eye Ball' was October 20th, but year-round production was possible by shading and lighting. Its flower was 3.1cm in diameter, and had 38.4 petals and 26.8 flowers per stem in autumn. Its petal was narrow (0.4 cm) and pink with green in color at the flower center. To flower in the short day condition, for 'Eye Ball' was about 47 days in spring, and 'Eye Ball' showed a vase life of 20.9 days in autumn. This cultivar was registered for a commercialization in 2007. 'Eye Ball' would be cultured under the greenhouse and rain shelter condition in Korea.

• Laboraroty Animal Research
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• v.34 no.4
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• pp.257-263
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• 2018

Trefoil factor 1 (TFF1, also known as pS2) is strongly expressed in the gastrointestinal mucosa and plays a critical role in the differentiation of gastric glands. Since approximately 50% of all human gastric cancers are associated with decreased TFF1 expression, it is considered a tumor suppressor gene. Tff1 deficiency in mice results in histological changes in the antral and pyloric gastric mucosa, with severe hyperplasia and dysplasia of epithelial cells, resulting in the development of antropyloric adenoma. Here, we generated Tff1-knockout (KO) mice, without a neomycin resistant ($Neo^R$) cassette, using the clustered regularly interspaced short palindromic repeats/CRISPR-associated nuclease 9 (CRSIPR/Cas9) system. Though our Tff1-KO mice showed phenotypes very similar to the previous embryonic stem (ES)-cell-based KO mice, they differed from the previous reports in that a reduction in body weight was observed in males. These results demonstrate that these newly established Tff1-KO mice are useful tools for investigating genetic and environmental factors influencing gastric cancer, without the effects of artificial gene insertion. Furthermore, these findings suggest a novel hypothesis that Tff1 expression influences gender differences.

• Journal of the Korean Wood Science and Technology
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• v.32 no.2
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• pp.26-32
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• 2004

Chemical compositions and chemical structure of lignin and alkali cooking condition and fiber length of red pepper were investigated and compared to those of woods. The chemical compositions of red pepper were higher component of extraction than that of wood. The contents of carbon and hydrogen of Klason lignin in red pepper were similar to that of pine and birch wood. On the other hand, the contents of oxygen and nitrogen of Klason lignin in the red pepper were higher than that of wood. The result of nitrobenzene oxidation shows that Klason lignin of red pepper was similar to lignin of softwood. The best alkali cooking condition of red pepper was 0.2%-anthraquinone, active alkali of 20% and liquor ratio of 1:7. The fiber length of red pepper was about 0.47 mm. Therefore, the red pepper fiber will be able to use special purpose of short fiber.

• Journal of Animal Reproduction and Biotechnology
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• v.37 no.4
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• pp.239-245
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• 2022

Protein and peptide candidates are screened to apply therapeutic application as a drug. Ensuring that these candidates are delivered and maximized effectiveness is still challenging and a variety of studies are ongoing. As drug delivery system vehicles, cell-penetrating peptide (CPP) can deliver various kinds of cargo into the cell cytosol. In a previous study, we developed Ara27 CPP, which are a zinc knuckle family protein of Arabidopsis, and confirmed internalization in human dermal fibroblasts and human dental pulp stem cells at low concentration with short time treatment condition without any toxicity. Ara27, an amphipathic CPP, could be modified and utilized in the biomedical field excluding the risk of toxicity. Therefore, we would like to confirm the non-toxic induced penetrating ability of Ara27 in various cell lines. The purpose of this study was to screen the cell internalization ability of Ara27 in various cell lines and to confirm Ara27 as a promising core CPP structure. First, Ara27 was screened to confirm non-toxicity concentration. Then, fluorescence-labeled Ara27 was treated on human normal cell lines, cancer cell lines and animal cell lines to identify the cellular internalization of Ara27. Ara27 was well intracellular localized in all cell lines and the intensity of fluorescence was remarkably increased in time pass manner. These results indicate that Ara27 has the potential as a core structure for applications in various drug delivery systems.

• Hip & pelvis
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• v.35 no.4
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• pp.246-252
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• 2023

Purpose: The aim of this study was to compare short-term results from use of the direct anterior approach (DAA) and the conventional posterolateral approach (PLA) in performance of bipolar hemiarthroplasty for treatment of femoral intertrochanteric fractures in elderly patients. Materials and Methods: A retrospective review of 100 patients with intertrochanteric fractures who underwent bipolar hemiarthroplasty was conducted. The PLA was used in 50 cases from 2016 to 2019; since that time we have used the DAA in 50 cases from 2019 to 2021. Measurements of mean operative time, blood loss, hospitalization period, and ambulation status, greater trochanter (GT) migration and stem subsidence were performed. And the incidence of complications was examined. Results: Operative time was 73.60±14.56 minutes in the PLA group and 79.80±8.89 minutes in the DAA group (P<0.05). However, after experiencing 20 cases using DAA, there was no statistically difference in operative time between two groups (P=0.331). Blood loss was 380.76±180.67 mL in the PLA group and 318.14± 138.51 mL in the DAA group (P<0.05). The hospitalization was 23.76±11.89 days in the PLA group and 21.45 ±4.18 days in the DAA group (P=0.207). In both groups, there were no progressive GT migration, intraoperative fractures or dislocations, although there was one case of infection in the PLA group. Conclusion: Although use of the DAA in performance of bipolar hemiarthroplasty required slightly more time in the beginning compared with the PLA, the DAA may well be an alternative, safe surgical technique as a muscle preserving procedure in elderly patients with intertrochanteric fractures.

• Journal of Animal Reproduction and Biotechnology
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• v.39 no.1
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• pp.19-30
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• 2024

Background: Although an understanding of the proliferation and differentiation of fish female germline stem cells (GSCs) is very important, an appropriate threedimensional (3D) research model to study them is not well established. As a part of the development of stable 3D culture system for fish female GSCs, we conducted this study to establish a 3D aggregate culture system of ovarian cells in marine medaka, Oryzias dancena. Methods: Ovarian cells were separated by Percoll density gradient centrifugation and two different cell populations were cultured in suspension to form ovarian cell aggregates to find suitable cell populations for its formation. Ovarian cell aggregates formed from different cell populations were evaluated by histology and gene expression analyses. To evaluate the media supplements, ovarian cell aggregate culture was performed under different media conditions, and the morphology, viability, size, gene expression, histology, and E2 secretion of ovarian cell aggregates were analyzed. Results: Ovarian cell aggregates were able to be formed well under specific culture conditions that used ultra-low attachment 96 well plate, complete mESM2, and the cell populations from top to 50% layers after separation of ovarian cells. Moreover, they were able to maintain minimal ovarian function such as germ cell maintenance and E2 synthesis for a short period. Conclusions: We established basic conditions for the culture of O. dancena ovarian cell aggregates. Additional efforts will be required to further optimize the culture conditions so that the ovarian cell aggregates can retain the improved ovarian functions for a longer period of time.

• Journal of Korean Society of Forest Science
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• v.97 no.6
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• pp.605-610
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• 2008

This study was carried out to investigate the short-term (3 years) effect of thinning on aboveground carbon storage for 34-year-old (site 1) and 45-year-old (site 2) Korean pine (Pinus koraiensis Siebold et Zuccarini) plantations with different diameter class and site quality located in Gwangneung experimental forest. Thinning was manually carried out in consideration of basal area in 2004 (site 1 : 30% and 60% of basal area removed and site 2 : 60% of basal area removed). In 2004 and 2007, DBH and tree height were measured to analyze the changes in carbon storage after thinning. In the sites of 60% of basal area removed, although the mean DBH of site 1 was higher than that of site 2, mean annual carbon storage increment in site 2 ($6.5Mg\;C\;ha^{-1}yr^{-1}$) was about 3 times higher than that in site 1 ($2.3Mg\;C\;ha^{-1}yr^{-1}$). The reason for this result was probably due to higher stem density and site quality in site 2 compared to site 1. In site 2, mean annual carbon storage increment in thinned plot ($6.5Mg\;C\;ha^{-1}yr^{-1}$) was about 1.3 times higher than that in control ($5.2Mg\;C\;ha^{-1}yr^{-1}$). The results suggest that the stem density and site quality may be much more related to the aboveground carbon storage compared to diameter class. In addition, it is needed to consider these two factors for determining whether thinning is a feasible management alternative for the increase in aboveground carbon sequestration.