• Korean journal of applied entomology
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• v.15 no.1 s.26
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• pp.23-27
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• 1976

Screenhouse experiments were conducted to find out the varietal difference in ovipositional preference of the striped rice borer moths (Chilo suppressalis W.) on eight varieties of rice and its association with several plant morphological characters. The results indicated the strong preference of the moths for ovipositing on several varieties. The varieties Tongil (Suweon 213-1), Yushin, Jinheung and TKM-6 had comparatively more egg masses and more eggs than the varieties IR-26, IR-747, Rexoro and Juckna. The strong ovipositional preference of moths of Tongil, as compared to the resistant variety TKM-6 and the susceptible variety Rexoro, was. always consistent in this study whenever the number of tillers were uniformed and each two varieties were paired. It could be concluded that the moths definitely preferred Tongil among the test varieties. There was not any significant correlations between the ovipositional preference and the plant characters; such as number of tillers per plant, plant height, external diameter of stem, and size of leaf. The cause of strong perference of the moths for ovipositing on short type varieties Tongil and Yushin were still suspectable.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.48 no.6
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• pp.490-494
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• 2003

To understand flowering response under diverse day-length condition and the movement of floral stimulus, we used six perilla accessions which showed diverse days to flowering. Though the growth phase was reported as irreversible between vegetative and reproductive stage, perilla showed reversible growth phase according to day length increase in May and June. When it sowed in March, flowering response was started in early May and vegetative and reproductive phase was coexisted in late June. When a part of a perilla plant was treated under short day condition, only apical buds on main stem or branches were flowered but other long day conditioned apical buds were not flowered. With this result it is suggested that the floral stimulus can not be transferred to other part of perilla.

• KSBB Journal
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• v.25 no.3
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• pp.215-222
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• 2010

Monogenic diseases are resulted from modifications in a single gene of human cells. Because their treatment with pharmacological medicine have a temporary effect, continuous nursing care and retreatment are required. Gene therapy, gene targeting and induced pluripotent stem cell (iPSC) are considered permanent treatment methods of them. In gene therapy, however, retroviral vectors that have potential toxicity caused by random insertion of harmful virus are used as vehicles for transferring genetic materials. On the other hand, gene targeting could replace and remove the modified gene though homologous recombination (HR) induced by site-specific endonucleases. This short review provides a brief overview on the recently tailored endonucleses with high selectivity for HR.

• Journal of Embryo Transfer
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• v.30 no.4
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• pp.299-303
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• 2015

KO mice provide an excellent tool to determine roles of specific genes in biomedical filed. Traditionally, knockout mice were generated by homologous recombination in embryonic stem cells. Recently, engineered nucleases, such as zinc finger nuclease, transcription activator-like effector nuclease and clustered regularly interspaced short palindromic repeats (CRISPR), were used to produce knockout mice. This new technology is useful because of high efficiency and ability to generate biallelic mutation in founder mice. Until now, most of knockout mice produced using engineered nucleases were C57BL/6 strain. In the present study we used CRISPR-Cas9 system to generate knockout mice in FVB strain. We designed and synthesized single guide RNA (sgRNA) of CRISPR system for targeting gene, Abtb2. Mouse zygote were obtained from superovulated FVB female mice at 8-10 weeks of age. The sgRNA was injected into pronuclear of the mouse zygote with recombinant Cas9 protein. The microinjected zygotes were cultured for an additional day and only cleaved embryos were selected. The selected embryos were surgically transferred to oviduct of surrogate mother and offsprings were obtained. Genomic DNA were isolated from the offsprings and the target sequence was amplified using PCR. In T7E1 assay, 46.7% among the offsprings were founded as mutants. The PCR products were purified and sequences were analyzed. Most of the mutations were founded as deletion of few sequences at the target site, however, not identical among the each offspring. In conclusion, we found that CRISPR system is very efficient to generate knockout mice in FVB strain.

• Parasites, Hosts and Diseases
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• v.51 no.4
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• pp.441-448
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• 2013

This study describes the first record of Bourgelatia diducta (Nematoda: Chabertiidae) from wild boars in the Republic of Korea (=South Korea). Gastrointestinal tracts of 87 Korean wild boars (Sus scrofa coreanus) hunted in mountains in the south-western part of South Korea between 2009 and 2012 were examined for their visceral helminths. B. diducta, as identified by morphological characteristics of the head and tail, were recovered from the large intestine of 47 (54%) wild boars. The average length of adult female worms was $11.3{\pm}0.872$ mm and the thickest part of the body measured $0.54{\pm}0.04$ mm in maximum width, while those of males were $9.8{\pm}0.72$ and $0.45{\pm}0.03$ mm, respectively. The characteristic J-shaped type II ovejector was observed in females, and the type II dorsal ray with 2 rami on each side of the median fissure was uniquely seen in males. The buccal capsule was small, relatively thin-walled, cylindrical, very short, and ring-shaped. The externodorsal ray arose from a common stem with the dorsal ray. The cervical groove was absent. The anterior extremity was equipped with 20-22 external corona radiata, 4 cephalic papillae and 2 lateral amphids around the mouth. The eggs were $66.0{\times}38.9{\mu}m$ in average size. By the present study, B. diducta (Nematoda: Chabertiidae) is recorded for the first time in South Korea. Additionally, morphological characteristics and identification keys provided in the present study will be helpful in the faunistic or taxonomic studies for strongylid nematodes related.

• Korean Journal of Environmental Agriculture
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• v.18 no.1
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• pp.1-5
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• 1999

This study was conducted to investigate methane emission among rice ecotypes in paddy soil (Jeonbug series). The varieties of rice involved early maturing short-grain variety, Samcheonbyeo, middle maturing variety, Hawsungbyeo and late maturing variety, Dongjinbyeo. The seasonal change of methane flux was high from ear formation stage to heading stage. It was found that the methane flux was tended to be lower in early maturing variety than in the mid-to-late maturing variety. The plots without rice straw reduced methane emission as much as 46% relative to rice straw application. The methane emission rate through rice stem was highly correlated with temperature fluctuation. Methane emission on ecotypes by rice straw application was 0.394 g $m^{-2}day^{-1}$ in early maturing variety (Samcheonbyeo), 0.407 g $m^{-2}day^{-1}$ in mid maturing variety(Hawsungbyeo), 0.411 g $m^{-2}day^{-1}$ in late maturing variety(Dongjinbyeo).

• Journal of Ginseng Research
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• v.23 no.2 s.54
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• pp.88-92
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• 1999

Hydrolysis properties of ginseng saponins in processing of extraction with vacuum impulse system extraction method were compared with multi-stage extraction methods. Crude saponin content of the extract produced by vacuum impulse system extraction method was $11.5\%,$ compared with multi-stage extraction method (about $8.13\%).$ Also the yield of the extract increased about $6.7\%.$ The flavor and aroma of ginseng extract with vacuum impulse system extraction method are stronger than multi-stage extraction methods and people have a tendency to like more. The color was similar to existing extraction items and the liquidity ratio was high. Vacuum impulse system extraction method could save human resources because of short extraction time and automatic operation of processing. With HPLC pattern, We could ascertain the truth that hydrolysis properties of ginseng saponin was restrained in the extraction processing, vacuum impulse system extraction method.

• Molecules and Cells
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• v.39 no.8
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• pp.603-610
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• 2016

Recently, an increasing number of studies have focused on the effects of CD4+ T cell on cognitive function. However, the changes of Th2 cytokines in restricted CD4+ T cell receptor (TCR) repertoire model and their effects on the adult hippocampal neurogenesis and memory are not fully understood. Here, we investigated whether and how the mice with restricted CD4+ repertoire TCR exhibit learning and memory impairment by using OT-II mice. OT-II mice showed decreased adult neurogenesis in hippocampus and short- and long- term memory impairment. Moreover, Th2 cytokines in OT-II mice are significantly increased in peripheral organs and IL-4 is significantly increased in brain. Finally, IL-4 treatment significantly inhibited the proliferation of cultured adult rat hippocampal neural stem cells. Taken together, abnormal level of Th2 cytokines can lead memory dysfunction via impaired adult neurogenesis in OT-II transgenic.

• Korean Journal of Plant Resources
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• v.33 no.4
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• pp.398-403
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• 2020

Plagiothecium platyphyllum Mönk (Plagiotheciaceae), a species unrecorded previously in the moss flora of the Republic of Korea (South Korea), was collected in the Surak stream of Mt. Daedun in Jeollabuk-do Wanju-gun Sanbuk-ri. This species was known to be distributed around China, Japan, North Korea, and Europe. The morphological characters of P. platyphyllum's leaf are ovate-lanceolate, and mostly asymmetric, 2.0~2.5 × 1.0~1.4 mm. The margin of the leaf is entirely below and weakly serrulated near the apex. The costae parts are double, unequal, with one branch reaching to the mid-leaf. Median leaf cells are linear-rhomboidal, 80~127 × 10~16 ㎛ and alar cells are rectangular. Central strand is developed in the core of the stem.

• Horticultural Science & Technology
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• v.30 no.3
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• pp.325-328
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• 2012

A new Dendranthema grandiflourm 'My Sun' was developed at Gyeonggi-do Agricultural Research & Extension Services (GARES), Korea in 2009. 'My Sun' was initially derived from the cross in 2005 between 'Omega Time Orange', a potted chrysanthemum cultivar with orange single type, and 'Tasman', a potted chrysanthemum cultivar with white single type in 2005. The cultivar has single type flowers with yellow petals. Trial and evaluation was conducted from 2006 to 2009 for the selection of this variety, including a shading culture in spring and a retarding culture in winter. The flowering time of 'My Sun' was October 13th, and year-round flowering is possible by shading or lighting treatment. The diameter of flower is 21.0 mm. Numbers of flowers per stem and petals per flower are 34.4 and 20.4, respectively. Its leaf color was green (Green Group 137A) and plant height was 13.3 cm. Days to flowering under the short day treatment is about 42 in spring, and numbers of branch per plant was 3.4 ea in the winter. This cultivar was resistance to white rust and consumer's preference of new pot-mum is high level than control.