• 한국소음진동공학회논문집
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• 제22권12호
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• pp.1227-1236
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• 2012

The low speed machinery faults are usually caused by the bearing failure of the rolling elements. As the life time of the bearing is limited, the condition monitoring of bearing is very important to maintain the continuous operation without failures. A few monitoring techniques using time domain, frequency domain and fuzzy neural network vibration analysis are introduced to detect and diagnose the faults of the low speed machinery. This paper presents a method of fault detection for the rolling element bearing in the low speed machinery using the Wiener filtering and shock pulse counting techniques. Wiener filter is used for noise cancellation and it clearly makes the shock pulse emerge from the time signal with the high level of noise. The shock pulse counting is used to determine the various faults obviously from the shock signal with transient pulses not related with the bearing fault. Machine fault simulator is used for the experimental measurement in order to verify this technique is the powerful tool for the low speed machine compared with the frequency analysis. The test results show that the method proposed is very effective parameter even for the signal with high contaminated noise, speed variation and very low energy. The presented method shows the optimal tool for the condition monitoring purpose to detect the various bearing fault with high accuracy.

• Asian Pacific Journal of Cancer Prevention
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• 제15권20호
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• pp.8947-8950
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• 2014

Immunological functions of heat shock proteins (HSPs) have long been recognized. In this study we aimed to efficiently purify HSP70 from renal cell carcinoma and test it as a tumor antigen for pulsing dendritic cells in vitro. HSP70 was purified from renal cell carcinoma specimens by serial column chromatography on Con A-sepharose, PD-10, ADP-agarose and DEAE-cellulose, and finally subjected to fast protein liquid chromatography (FPLC). Dendritic cells derived from the adherent fraction of peripheral blood mononuclear cells were cultured in the presence of IL-4 and GM-CSF and exposed to tumor HSP70. After 24 hours, dendritic cells were phenotypically characterized by flow cytometry. T cells obtained from the non-adherent fraction of peripheral blood mononuclear cells were then co-cultured with HSP70-pulsed dendritic cells and after 3 days T cell cytotoxicity towards primary cultured renal cell carcinoma cells was examined by Cell Counting Kit-8 assay. Dendritic cells pulsed in vitro with tumor-derived HSP70 expressed higher levels of CD83, CD80, CD86 and HLA-DR maturation markers than those pulsed with tumor cell lysate and comparable to that of dendritic cells pulsed with tumor cell lysate plus TNF-${\alpha}$. Concomitantly, cytotoxic T-lymphocytes induced by HSP70-pulsed dendritic cells presented the highest cytotoxic activity. There were no significant differences when using homologous or autologous HSP70 as the tumor antigen. HSP70 can be efficiently purified by chromatography and induces in vitro dendritic cell maturation in the absence of TNF-${\alpha}$. Conspecific HSP70 may effectively be used as a tumor antigen to pulse dendritic cells in vitro.