• 한국항만경제학회지
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• 제32권4호
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• pp.165-182
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• 2016

본 논문은 동아시아의 주요 컨테이너항만 간의 경쟁 및 보완 관계를 분석하는 데 그 의의가 있다. 분석의 대상이 되는 항만들은 컨테이너 기종점 물동량 규모로 세계 최대인 중국의 6개 주요 항만과 환적 물동량 규모로 세계 최대인 싱가포르, 홍콩, 부산의 항만이며, 지리적으로는 동아시아에 밀집된 항만들이다. 본 연구에서는 2008년부터 2015년까지의 월간 컨테이너 물동량에 대한 시계열 자료를 이용하여 항만 간의 동태적 관계를 분석하였으며, 벡터오차수정모형(Vector Error Correction Model)에 기초하여 다음과 같이 분석하였다. 먼저 그랜저 인과관계 검정을 통해 항만 간의 상호관계를 규명하고자 하였다. 그리고 이어서 공적뿐 검정을 통해 관련 있는 두 항만 간의 장기적인 균형관계를 살펴보고자 했으며, 아울러 충격반응함수 및 표준변차의 분산분해 과정을 통해 단기적인 영향에 대해서 밝히고자 하였다. 결과를 살펴보면, 먼저 그랜저 인과관계 검정과 공적뿐 검정의 결과, 부산과 홍콩 및 싱가포르의 환적 물동량은 중국 내륙의 주요 기종점 물동량에 대해 상호적으로 또는 일방적으로 경쟁적인 관계가 있음을 보여주었다. 그러나 환적 물동량 간의 인과관계는 상호적으로는 유의미하게 나타나지 않았으며, 공적분도 존재하지 않았다. 다만 분산분해의 결과를 통해 단기적이고 부분적으로 충격에 차지하는 비중을 확인해 본 결과 홍콩항의 환적 물동량이 부산항과 싱가포르항의 환적 물동량에 차지하는 비중이 높아지고 있음을 알 수 있었다. 충격반응함수의 결과를 종합해 보면, 분석의 대부분에서 2기가 지나는 시점에 충격의 여파가 최대가 되며, 6기가 지나면서 그 충격은 소멸되었다. 본 연구에서 분석한 결과로 확인할 수 있는 것은 환적 항만 간의 관계가 경쟁적이라고 할 수 없다는 사실과 오히려 기종점 항만과 환적 항만의 경쟁 관계가 발생한다는 것이다. 이것은 기종점 물동량에 의해 파생된 환적 물동량이 기종점 물동량의 증가에 따라 항상 증가하지 않으며 오히려 감소될 수 있음을 설명하고 있다.

• Journal of Microbiology and Biotechnology
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• 제26권11호
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• pp.1881-1890
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• 2016

Manganese superoxide dismutase (MnSOD) is a vital enzyme that protects cells from free radicals through eliminating superoxide radicals ($O^{2-}$). Hirudin, a kind of small active peptide molecule, is one of the strongest anticoagulants that can effectively cure thrombus diseases. In this study, we fused Hirudin to the C terminus of human MnSOD with the GGGGS linker to generate a novel dual-feature fusion protein, denoted as hMnSOD-Hirudin. The hMnSOD-Hirudin gene fragment was cloned into the pET15b (SmaI, CIAP) vector, forming a recombinant pET15b-hMnSOD-Hirudin plasmid, and then was transferred into Escherichia coli strain Rosetta-gami for expression. SDS-PAGE was used to detect the fusion protein, which was expected to be about 30 kDa upon IPTG induction. Furthermore, the hMnSOD-Hirudin protein was heavily detected as a soluble form in the supernatant. The purification rate observed after Ni NTA affinity chromatography was above 95%. The hMnSOD-Hirudin protein yield reached 67.25 mg per liter of bacterial culture. The identity of the purified protein was confirmed by western blotting. The hMnSOD-Hirudin protein activity assay evinced that the antioxidation activity of the hMnSOD-Hirudin protein obtained was $2,444.0{\pm}96.0U/mg$, and the anticoagulant activity of the hMnSOD-Hirudin protein was $599.0{\pm}35.0ATU/mg$. In addition, in vitro bioactivity assay showed that the hMnSOD-Hirudin protein had no or little cytotoxicity in H9c2, HK-2, and H9 (human $CD_4{^+}$, T cell) cell lines. Transwell migration assay and invasion assay showed that the hMnSOD-Hirudin protein could suppress human lung cancer 95-D cell metastasis and invasion in vitro.

• 디지털융복합연구
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• 제11권11호
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• pp.191-196
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• 2013

중국의 전체 산업 중 기업 수를 토대로 볼 때 중소기업이 차지하는 비중은 99%이고, 종업원 수는 80%를 차지하며, 중국 국내총생산(GDP)의 60%를 차지하고 있어 중국 내 중소기업은 중국의 경제성장에 매우 중요한 영향을 미치고 있다. 이러한 시점에서 본 논문은 중국 심천증권거래소에 상장된 기업들 중 제조업에 속하는 45개 중소기업들을 대상으로 다중회귀분석을 이용하여 자본구조가 어떠한 요인들에 의하여 결정되는지를 확인하고자 하는데 연구목적이 있다. 본 연구결과를 요약하면 다음과 같다. 첫째, 성장성은 부채비율에 1% 유의수준에서 유의적인 정(+)의 영향을 미치는 것으로 나타났다. 둘째, 수익성은 부채비율에 1% 유의수준에서 부(-)의 영향을 미치는 것으로 나타났다. 셋째, 기업규모는 부채비율에 영향을 미치지 않는 것으로 나타났다. 넷째, 담보가치, 비부채성 절세효과는 부채비율에 약한 정(+)의 영향을 미치는 것으로 나타났으나 통계적으로 유의하지 않았다. 본 연구는 투자자들에 대한 정보제공과 중국 중소기업의 재무구조 개선을 위한 기초자료로 활용될 수 있다는 점에서 의의가 있다.

• Journal of Microbiology and Biotechnology
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• 제29권10호
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• pp.1644-1655
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• 2019

Saccharomyces cerevisiae (S. cerevisiae) and glucagon-like peptide-2 (GLP-2) have been employed to improve the intestinal development of weaned animals. The goal of this study was to determine whether either exogenous S. cerevisiae or GLP-2 elicits major effects on fecal microbiotas and cytokine responses in weaned piglets. Ninety-six piglets weaned at 26 days were assigned to one of four groups: 1) Basal diet (Control), 2) empty vector-harboring S. cerevisiae (EV-SC), 3) GLP-2-expressing S. cerevisiae (GLP2-SC), and 4) recombinant human GLP-2 (rh-GLP2). At the start of the post-weaning period (day 0), and at day 28, fecal samples were collected to assess the bacterial communities via sequencing the V1-V2 region of the 16S-rRNA gene, and piglets' blood was also sampled to measure cytokine responses (i.e., IL-$1{\beta}$, TNF-${\alpha}$, and IFN-${\gamma}$). This study revealed that, on the one hand, although S. cerevisiae supplementation did not significantly alter the growth of weaned piglets, it induced increases in the relative abundances of two core genera (Ruminococcaceae_norank and Erysipelotrichaceae_norank) and decreases in the relative abundances of two other core genera (Lachnospiraceae_norank and Clostridiale_norank) and cytokine levels (IL-$1{\beta}$ and TNF-${\alpha}$) (p < 0.05, Control vs EV-SC; p < 0.05, rh-GLP2 vs GLP2-SC). On the other hand, GLP-2 supplementation had no significant influence on fecal bacterial communities and cytokine levels, but it produced better body weight and average daily gain (p < 0.05, Control vs EV-SC; p < 0.05, rh-GLP2 vs GLP2-SC). Therefore, altered fecal microbiotas and cytokine response effects in weaned piglets were due to S. cerevisiae rather than GLP-2.

• Nuclear Engineering and Technology
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• 제53권8호
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• pp.2534-2546
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• 2021

Nuclear emergency preparedness and response is an essential part to ensure the safety of nuclear power plant (NPP). Key support technologies of nuclear emergency decision-making usually consist of accident diagnosis, source term estimation, accident consequence assessment, and protective action recommendation. Source term estimation is almost the most difficult part among them. For example, bad communication, incomplete information, as well as complicated accident scenario make it hard to determine the reactor status and estimate the source term timely in the Fukushima accident. Subsequently, it leads to the hard decision on how to take appropriate emergency response actions. Hence, this paper aims to develop a method for rapid source term estimation to support nuclear emergency decision making in pressurized water reactor NPP. The method aims to make our knowledge on NPP provide better support nuclear emergency. Firstly, this paper studies how to build a Bayesian network model for the NPP based on professional knowledge and engineering knowledge. This paper presents a method transforming the PRA model (event trees and fault trees) into a corresponding Bayesian network model. To solve the problem that some physical phenomena which are modeled as pivotal events in level 2 PRA, cannot find sensors associated directly with their occurrence, a weighted assignment approach based on expert assessment is proposed in this paper. Secondly, the monitoring data of NPP are provided to the Bayesian network model, the real-time status of pivotal events and initiating events can be determined based on the junction tree algorithm. Thirdly, since PRA knowledge can link the accident sequences to the possible release categories, the proposed method is capable to find the most likely release category for the candidate accidents scenarios, namely the source term. The probabilities of possible accident sequences and the source term are calculated. Finally, the prototype software is checked against several sets of accident scenario data which are generated by the simulator of AP1000-NPP, including large loss of coolant accident, loss of main feedwater, main steam line break, and steam generator tube rupture. The results show that the proposed method for rapid source term estimation under nuclear emergency decision making is promising.

• Journal of Veterinary Science
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• 제23권4호
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• pp.55.1-55.12
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• 2022

Background: ASF was first reported in Kenya in 1910 in 1921. In China, ASF spread to 31 provinces including Henan and Jiangsu within six months after it was first reported on August 3, 2018. The epidemic almost affected the whole China, causing direct economic losses of tens of billions of yuan. Cause great loss to our pig industry. As ELISA is cheap and easy to operate, OIE regards it as the preferred serological method for ASF detection. P54 protein has good antigenicity and is an ideal antigen for detection. Objective: To identify a conservative site in the African swine fever virus (ASFV) p54 protein and perform a Cloth-enzyme-linked immunosorbent assay (ELISA) for detecting the ASFV antibody in order to reduce risks posed by using the live virus in diagnostic assays. Method: We used bioinformatics methods to predict the antigen epitope of the ASFV p54 protein in combination with the antigenic index and artificially synthesized the predicted antigen epitope peptides. Using ASFV-positive serum and specific monoclonal antibodies (mAbs), we performed indirect ELISA and blocking ELISA to verify the immunological properties of the predicted epitope polypeptide. Results: The results of our prediction revealed that the possible antigen epitope regions were A23-29, A36-45, A72-94, A114-120, A124-130, and A137-150. The indirect ELISA showed that the peptides A23-29, A36-45, A72-94, A114-120, and A137-150 have good antigenicity. Moreover, the A36-45 polypeptide can react specifically with the mAb secreted by hybridoma cells, and its binding site contains a minimum number of essential amino acids in the sequence 37DIQFINPY44. Conclusions: Our study confirmed a conservative antigenic site in the ASFV p54 protein and its amino acid sequence. A competitive ELISA method for detecting ASFV antibodies was established based on recombinant p54 and matching mAb. Moreover, testing the protein sequence alignment verified that the method can theoretically detect antibodies produced by pigs affected by nearly all ASFVs worldwide.

• The Korean Journal of Physiology and Pharmacology
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• 제26권6호
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• pp.541-556
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• 2022

Myocardial fibrosis is a key link in the occurrence and development of diabetic cardiomyopathy. Its etiology is complex, and the effect of drugs is not good. Cardiomyocyte apoptosis is an important cause of myocardial fibrosis. The purpose of this study was to investigate the effect of gaseous signal molecule sulfur dioxide (SO₂) on diabetic myocardial fibrosis and its internal regulatory mechanism. Masson and TUNEL staining, Western-blot, transmission electron microscopy, RT-qPCR, immunofluorescence staining, and flow cytometry were used in the study, and the interstitial collagen deposition, autophagy, apoptosis, and changes in phosphatidylinositol 3-kinase (PI3K)/AKT pathways were evaluated from in vivo and in vitro experiments. The results showed that diabetic myocardial fibrosis was accompanied by cardiomyocyte apoptosis and down-regulation of endogenous SO₂-producing enzyme aspartate aminotransferase (AAT)_1/2. However, exogenous SO₂ donors could up-regulate AAT_1/2, reduce apoptosis of cardiomyocytes induced by diabetic rats or high glucose, inhibit phosphorylation of PI3K/AKT protein, up-regulate autophagy, and reduce interstitial collagen deposition. In conclusion, the results of this study suggest that the gaseous signal molecule SO₂ can inhibit the PI3K/AKT pathway to promote cytoprotective autophagy and inhibit cardiomyocyte apoptosis to improve myocardial fibrosis in diabetic rats. The results of this study are expected to provide new targets and intervention strategies for the prevention and treatment of diabetic cardiomyopathy.

• 서비스연구
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• 제13권4호
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• pp.70-96
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• 2023

최근 간호인력들의 높은 이직률과 직무스트레스 증가로 인한 문제가 사회적 이슈로 크게 부각되며, 간호 조직 내의 '태움' 문제에 대한 관심이 증가하고 있다. 이에 따라, 본 연구는 간호업무 현장에서의 괴롭힘을 포함한 태움 문제에 대한 해결책 모색이 시급한 상황에서 이 문제에 대한 예방 및 극복방안을 제시하는 것을 목적으로 한다. 이를 위해 S-O-R 프레임워크 및 선행연구를 기반으로 직무스트레스 및 이직의도를 태움의 결과변수로, 커뮤니케이션능력을 선행요인으로 도출하였으며, 이러한 태움의 영향 메커니즘에서 마음챙김과 사회적지지가 문제 극복을 돕는 조절변수 역할을 수행할 것으로 보고 연구모형을 구성하였다. 최근 1년 내 태움을 경험한 간호인력 300명을 대상으로 한 설문조사를 통해 자료를 수집하고, 구조방정식모형을 활용하여 가설을 검증한 결과, 간호인력의 커뮤니케이션능력이 높을수록 태움 피해를 적게 인지하는 것으로 나타났으며, 태움으로 인한 피해는 높은 직무스트레스를 거쳐 이직의도로 이어진다는 사실을 밝혀내었다. 또한, 마음챙김과 사회적지지는 각각 태움이 직무스트레스에, 직무스트레스가 이직의도에 미치는 정(+)의 영향을 유의하게 약화시키는 것으로 나타났다. 본 연구는 기존의 S-O-R 프레임워크에서 사전예방 단계를 추가하여 확장된 P-S-O-R 프레임워크를 제안하였으며, 추가로 마음챙김과 사회적지지 변수의 조절효과를 검증했다는 점에서 연구의 의의가 있다. 본 연구결과를 통해 실무에 적용 가능한 태움 예방 및 극복방안 마련을 위한 구체적인 지침을 제시할 수 있기를 기대한다.

• Korean Journal of Radiology
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• 제20권1호
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• pp.83-93
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• 2019

Objective: The purpose of this study was to prospectively investigate the value of the myocardial extracellular volume fraction (ECV) in predicting myocardial functional outcome after revascularization of coronary chronic total occlusion (CTO). Materials and Methods: Thirty patients with CTO underwent cardiovascular magnetic resonance (CMR) before and 6 months after revascularization. Three baseline markers of functional outcome were evaluated in the dysfunctional segments assigned to the CTO vessels: ECV, transmural extent of infarction (TEI), and unenhanced rim thickness (RIM). At the global level, the ECV values of the whole myocardium with and without a hyperenhanced region (global and remote ECV) were respectively measured. Results: In per-segment analysis, ECV was superior to TEI and RIM in predicting functional recovery (area under receiver operating characteristic curve [AUC]: 0.86 vs. 0.75 and 0.73, all p values < 0.010), and it emerged as the only independent predictor of regional functional outcome (odds ratio [OR] = 0.83, 95% confidence interval [CI]: 0.77-0.89; p < 0.001) independent of collateral circulation. In per-patient analysis, global baseline ECV was indicative of ejection fraction (EF) at the follow-up examination (β = -0.61, p < 0.001) and changes in EF (β = -0.57, p = 0.001) in multivariate regression analysis. A patient with global baseline ECV less than 30.0% (AUC, 0.93; sensitivity 94%, specificity 80%) was more likely to demonstrate significant EF improvement (OR: 0.38; 95% CI: 0.17-0.85; p = 0.019). Conclusion: Extracellular volume fraction obtained by CMR may provide incremental value for the prediction of functional recovery both at the segmental and global levels in CTO patients, and may facilitate the identification of patients who can benefit from revascularization.

• International Journal of Stem Cells
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• 제16권3호
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• pp.326-341
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• 2023

Background and Objectives: Osteoarthritis (OA) is a degenerative disease that leads to the progressive destruction of articular cartilage. Current clinical therapeutic strategies are moderately effective at relieving OA-associated pain but cannot induce chondrocyte differentiation or achieve cartilage regeneration. We investigated the ability of wedelolactone, a biologically active natural product that occurs in Eclipta alba (false daisy), to promote chondrogenic differentiation. Methods and Results: Real-time reverse transcription-polymerase chain reaction, immunohistochemical staining, and immunofluorescence staining assays were used to evaluate the effects of wedelolactone on the chondrogenic differentiation of mesenchymal stem cells (MSCs). RNA sequencing, microRNA (miRNA) sequencing, and isobaric tags for relative and absolute quantitation analyses were performed to explore the mechanism by which wedelolactone promotes the chondrogenic differentiation of MSCs. We found that wedelolactone facilitates the chondrogenic differentiation of human induced pluripotent stem cell-derived MSCs and rat bone-marrow MSCs. Moreover, the forkhead box O (FOXO) signaling pathway was upregulated by wedelolactone during chondrogenic differentiation, and a FOXO1 inhibitor attenuated the effect of wedelolactone on chondrocyte differentiation. We determined that wedelolactone reduces enhancer of zeste homolog 2 (EZH2)-mediated histone H3 lysine 27 trimethylation of the promoter region of FOXO1 to upregulate its transcription. Additionally, we found that wedelolactone represses miR-1271-5p expression, and that miR-1271-5p post-transcriptionally suppresses the expression of FOXO1 that is dependent on the binding of miR-1271-5p to the FOXO1 3'-untranscribed region. Conclusions: These results indicate that wedelolactone suppresses the activity of EZH2 to facilitate the chondrogenic differentiation of MSCs by activating the FOXO1 signaling pathway. Wedelolactone may therefore improve cartilage regeneration in diseases characterized by inflammatory tissue destruction, such as OA.