• 소성∙가공
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• 제14권3호
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• pp.263-268
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• 2005

Heating channel layout design and evaluation technology for SMC molding system was investigated in this work. Traditional rules of cooling channel design in injection molding were applied to the present work. Finite element thermal analysis with $ANSYS^{TM}$ was performed to evaluate the temperature distribution of SHC mold surface. SMC mold was manufactured to evaluate the effect of a proposed heating channel layout system on the temperature distribution of SMC mold surface and infrared camera was applied to a measurement of temperature distribution. It was shown that infrared camera application was possible in a measurement of temperature distribution on SHC mold surface.

• Asian Pacific Journal of Cancer Prevention
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• 제14권10호
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• pp.5645-5650
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• 2013

Src homology 2 domain containing (SHC) is a proto-oncogene which mediates cell proliferation and carcinogenesis in human carcinomas. Here, the SHC SH2-domain binding protein 1 (SHCBP1) was first established to be up-regulated in human hepatocellular carcinoma (HCC) tissues by array-base comparative genome hybridization (aCGH). Meanwhile, we examine and verify it by quantitative real-time PCR and western blot. Our current data show that SHCBP1 was up-regulated in HCC tissues. Overexpression of SHCBP1 could significantly promote HCC cell proliferation, survival and colony formation in HCC cell lines. Furthermore, knockdown of SHCBP1 induced cell cycle delay and suppressed cell proliferation. Furthermore, SHCBP1 could regulate the expression of activate extracellular signal-regulated kinase 1/2 (ERK1/2) and cyclin D1. Together, our findings indicate that SHCBP1 may contribute to human hepatocellular carcinoma by promoting cell proliferation and may serve as a molecular target of cancer therapy.

• The Korean Journal of Physiology and Pharmacology
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• 제21권4호
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• pp.377-384
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• 2017

Activation of protein kinase C (PKC) is closely linked with endothelial dysfunction. However, the effect of $PKC{\beta}II$ on endothelial dysfunction has not been characterized in cultured endothelial cells. Here, using adenoviral $PKC{\beta}II$ gene transfer and pharmacological inhibitors, the role of $PKC{\beta}II$ on endothelial dysfucntion was investigated in cultured endothelial cells. Phorbol 12-myristate 13-acetate (PMA) increased reactive oxygen species (ROS), p66shc phosphorylation, intracellular adhesion molecule-1, and monocyte adhesion, which were inhibited by $PKC{\beta}i$ (10 nM), a selective inhibitor of $PKC{\beta}II$. PMA increased the phosphorylation of CREB and manganese superoxide dismutase (MnSOD), which were also inhibited by $PKC{\beta}i$. Gene silencing of CREB inhibited PMA-induced MnSOD expression, suggesting that CREB plays a key role in MnSOD expression. Gene silencing of $PKC{\beta}II$ inhibited PMA-induced mitochondrial ROS, MnSOD, and ICAM-1 expression. In contrast, overexpression of $PKC{\beta}II$ using adenoviral $PKC{\beta}II$ increased mitochondrial ROS, MnSOD, ICAM-1, and p66shc phosphorylation in cultured endothelial cells. Finally, $PKC{\beta}II$-induced ICAM-1 expression was inhibited by Mito-TEMPO, a mitochondrial ROS scavenger, suggesting the involvement of mitochondrial ROS in PKC-induced vascular inflammation. Taken together, the results suggest that $PKC{\beta}II$ plays an important role in PMA-induced endothelial dysfunction, and that the inhibition of $PKC{\beta}II$-dependent p66shc signaling acts as a therapeutic target for vascular inflammatory diseases.

• 한국식품영양과학회지
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• 제42권3호
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• pp.415-420
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• 2013

쌀의 기능성 소재화 및 새로운 용도 개발 연구의 일환으로, 제2형 당뇨환자의 혈당을 조절할 수 있는 인슐린 기능 개선 소재로 알려져 있는 cyclo histidine-proline(CHP) 함유 대두가수분해물(SHC)을 쌀에 함유시켜 신규 기능성 쌀을 개발하고자 하였다. 이를 위해 흡수 및 분무 코팅에 의해 쌀의 가공 중 첨가 기능성 성분인 CHP 함유된 대두가수분해물의 소실을 억제하는 기능성 성분 강화 코팅 쌀의 제조공정을 검토하였다. 흡수 코팅법은 CHP 함유 대두가수분해물의 농도 증가에 따라 흡수저해가 일어나 흡수율이 낮게 나타나므로 적합하지 못하였다. 반면 methylcellulose 폴리머를 사용한 분무 코팅에 의하여 제조된 코팅 쌀은 다소의 균열 발생 및 미황색화가 관찰되었으나 외관 형태의 큰 변화는 관찰되지 않았다. 특히, 수화양상 및 수화속도가 원료 쌀에 비하여 높게 나타났으며, 코팅과정 중의 건조에 의한 호화 등은 관찰되지 않았다. 따라서 5%(w/v)의 CHP 함유 대두가수분해물을 함유한 1%(w/v) methylcellulose 폴리머를 보호제로 사용하여 분무 코팅한 후 건조하여 CHP 함유 대두가수분해물을 기능성 성분으로 하는 기능성 코팅 쌀을 제조하는 공정을 확립하였다.

• BMB Reports
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• 제32권2호
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• pp.196-202
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• 1999

The existence of the Ras-independent signal transduction pathway of insulin leading to DNA synthesis was investigated in Rat-1 fibroblasts overexpressing human insulin receptor (HIRc-B) using the single-cell microinjection technique. Microinjection of a dominant-negative mutant $Ras^{N17}$ protein into quiescent HIRc-B cells inhibited the DNA synthesis stimulated by insulin. Microinjection of oncogenic H-$Ras^{V12}$ protein ($H-Ras^{V12}$) (0.1 mg/ml) induced DNA synthesis by 35%, whereas that of control-injected IgG was induced by 20%. When the marginal amount of oncogenic H-$Ras^{V12}$ protein was coinjected with a dominant-negative mutant of the H-Ras protein ($Ras^{N17}$), DNA synthesis was 35% and 74% in the absence and presence of insulin, respectively. This full recovery of DNA synthesis by insulin suggests the existence of the Ras-independent pathway. The same recovery was observed in the cells coinjected with either H-$Ras^{V12}$ plus H-$Ras^{N17}$ plus SH2 domain of the p85 subunit of PI3-kinase ($p85^{SH2-N}$) or H-$Ras^{V12}$ plus H-$Ras^{N17}$ plus interfering anti-Shc antibody. When co-injected with a dominant-negative H-$Ras^{N17}$, the DNA synthesis induced by the Ras-independent pathway was blocked. These results indicate that the Ras-independent pathway of insulin leading to DNA synthesis exists, bypassing the p85 of PI3-kinase and Shc protein, and requires Rac1 protein.

• 한국항해항만학회지
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• 제30권8호
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• pp.691-697
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• 2006

본 연구의 목적은 기존의 컨테이너터미널 이송차량의 기술대안을 분석하여 터미널 생산성을 높일 수 있는 고생산성이송차량 모델을 개발하는 것이다. 대안 개발을 위해 YT(Yard Tractor), S/C(Straddle Carrier), SHC(Shuttle Carrier), AGV(Automated Guided Vehicle) 등의 이송차량에 대한 기술적 사양을 분석하며, 이송차량의 기술단계별 세대를 분류하기 위하여 운영현황과 성능을 조사한다. 본 연구에서 제시하는 이송차량의 개발 대안은 향후 고생산성의 진보된 컨테이너 터미널에 유용하게 활용될 것이다.

• 한국항해항만학회:학술대회논문집
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• 한국항해항만학회 2006년도 춘계학술대회 및 창립 30주년 심포지엄(논문집)
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• pp.367-372
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• 2006

본 연구의 목적은 기존의 컨테이너터미널 이송차량의 기술대안을 분석하여 터미널 생산성을 높일 수 있는 새로운 이송차량 대안을 개발하는 것이다. 대안 개발을 위해 YT(Yard Trailer), S/C(Straddle Carrier), SHC(Shuttle Carrier), AGV(Automated Guided Vehicle) 등의 이송차량에 대한 기술적 사양을 분석하며, 이송차량의 기술단계별 세대를 분류하기 위하여 운영과 성능을 조사한다. 본 연구에서 제시하는 이송차량의 개발 대안은 향후 고생산성의 진보된 컨테이너 터미널에 유용 할 것이다.

• 한국응급구조학회지
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• 제19권3호
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• pp.69-85
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• 2015

Purpose: This study aimed to investigate the prevalence of musculoskeletal symptoms (MSSs) and their correlation with subjective health condition (SHC) among emergency medical technicians (EMTs). Methods: A self-reported questionnaire was disseminated to EMTs (N=257) at emergency medical institutions and fire stations. Results: The prevalence of MSSs was very high in the studied EMTs: 88.3% reported having MSSs. The most commonly affected site was the lower back (73.9%) followed by the neck (56.8%) and the shoulder (55.6%). MSSs were more common in women than in men, and its frequency increased with age. MSSs in the neck and shoulder were more increased in EMTs with < 1 year of service. MSSs in the hand / wrist / finger or leg / foot were significantly increased in EMTs of emergency medical institutions than those of fire stations. Job satisfaction was significantly lower in those with MSSs of the lower back. The subjective heath condition was decreased with age, marriage, or smoking. There was significant corelation between MSSs and SHC. Conclusion: This study suggests that MSSs are a reasonably common problem for EMTs, and that solutions need to be imposed such as some actual tasks or items related to work postures, work control, and work organization.

• The Korean Journal of Physiology and Pharmacology
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• 제19권3호
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• pp.229-234
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• 2015

Nafamostat mesilate (NM) is a serine protease inhibitor with anticoagulant and anti-inflammatory effects. NM has been used in Asia for anticoagulation during extracorporeal circulation in patients undergoing continuous renal replacement therapy and extra corporeal membrane oxygenation. Oxidative stress is an independent risk factor for atherosclerotic vascular disease and is associated with vascular endothelial function. We investigated whether NM could inhibit endothelial dysfunction induced by tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$ ). Human umbilical vein endothelial cells (HUVECs) were treated with TNF-${\alpha}$ for 24 h. The effects of NM on monocyte adhesion, vascular cell adhesion molecule-1 (VCAM-1) and intracellular adhesion molecule-1 (ICAM-1) protein expression, p38 mitogenactivated protein kinase (MAPK) activation, and intracellular superoxide production were then examined. NM ($0.01{\sim}100{\mu}g/mL$) did not affect HUVEC viability; however, it inhibited the increases in reactive oxygen species (ROS) production and p66shc expression elicited by TNF-${\alpha}$ (3 ng/mL), and it dose dependently prevented the TNF-${\alpha}$ -induced upregulation of endothelial VCAM-1 and ICAM-1. In addition, it mitigated TNF-${\alpha}$ -induced p38 MAPK phosphorylation and the adhesion of U937 monocytes. These data suggest that NM mitigates TNF-${\alpha}$ -induced monocyte adhesion and the expression of endothelial cell adhesion molecules, and that the anti-adhesive effect of NM is mediated through the inhibition of p66shc, ROS production, and p38 MAPK activation.

• 한국정보통신학회:학술대회논문집
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• 한국해양정보통신학회 2004년도 춘계종합학술대회
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• pp.339-341
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• 2004

Self Q-switching에 의한 분포궤환 색소레이저(DFDL: Distributed Feedback Dye Laser)로부터 80uJ의 단일 극초단 펄스를 얻고, 이를 2단의 증폭기와 betune cell에 의한 3단 증폭으로 높은 출력을 얻은 후 BBO를 사용, 자외선 영역의 제 2고조파를 얻었다.