• Korean Journal of Environment and Ecology
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• v.20 no.2
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• pp.188-199
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• 2006

The purpose of this study was to provide data for conservation management of Pinus densiflora forest by analyzing ecological characteristics and the change of Pinus densiflora community structure for fifteen years in Gayasan National Park. According to the results of TWINSPAN analysis of Pinus densiflora forest, there were twenty plots(unit: $500m^2$)and it was classified into seven community types. The dominant species were Pinus densiflora in canopy layer, Carpinus laxiflora, Quercus variabilis, Quercus serrata in the understory layer and Sasa borealis, Rhododendron mucronulatum, Rhus trichocarpa in the shrub layer. The index of shannon's diversity was from 0.6803 to 1.2559 per $500m^2$ and the range of species number was from twenty-seven to fourty. As a result of the change of Pinus densiflora forest structure, the importance percentage(I.P.) of Carpinus laxiflora, Quercus variabilis, Quercus serrata in the understory layer were increased for fifteen years. The index of shannon's diversity changed $0.2608{\sim}1.0124\;into\;0.5547{\sim}1.2567\;per\;500m^2$ and species number changed $14{\sim}26\;into\;26{\sim}34$ for fifteen year.

• Journal of Life Science
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• v.18 no.7
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• pp.907-911
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• 2008

Radish (Raphanus sativus L.) is one of very important crop plants in the world. It is very important to determine hybrid seed quality in the production of hybrid Brassica vegetable seeds to avoid unacceptable contamination with self-inbred (sib) seeds. The allozyme for evaluating seed purity in a commercial $F_1-hybrid$ radish cultivar is demonstrated. Three hundred sixty seeds from the male and female harvest were subsequently screened for seed purity using 27 isozyme loci. Especially, F1 hybrids of radish, Per-1 ($aa{\times}bb$), Lap-1 ($aa{\times}bb$), Est-1 ($aa{\times}bb$) were presented clear hybrid bands. Est-1 locus revealed that 15 (8.3%) seeds from the female harvest and 26 (14.4%) seeds from the male harvest were sibs. It maintains higher than average level of genetic diversity compared with their correspondent parents. Shannon's index of phenotypic diversity (I) of hybrids was the highest of all accessions (R. sativus L. cv. Daepeng, R. sativus L. cv. Backza, and their hybrids). The allozyme may lead to a better insight into the hybrid seed purity.

• Korean Journal of Plant Resources
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• v.19 no.1
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• pp.189-195
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• 2006

We investigated the genetic variation in Stewartia koreana Nakai by examining 61 I-SSR amplicons in 120 individuals distributed among six natural populations in Korea. The overall percentage of polymorphic I-SSR amplicons was 81.9% and mean number of amplicons per I-SSR primer was 12.2. Levels of genetic diversity within 6 populations were similar each other[Shannon's Index $0.358{\sim}0.467$(mean: 0.407)]. The Mt. Obong population had the highest level of genetic diversity and was most distinctive from the other populations. Most variation existed among individuals within population(88.2%). Genetic differentiation among populations(${\phi}_{ST}$) was 0.118. The UPGMA dendrogram based on the genetic distance failed in showing decisive geographic relationships.

• KIEAE Journal
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• v.14 no.5
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• pp.49-57
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• 2014

This research is about analysis and evaluation of biodiversity of Suwon's street tree, then understanding the problem of it and finally finding a solution. Because the increasing damage of the street trees by disease and insects, insecticide is applied to prevent further damage. However, this insecticide is found to be cancer genic and causing hygienic threat to civilians. Therefore, by gathering Suwon's internal statistics about Suwon's street tree, the trees are divided into three categories, tall evergreen trees, deciduous trees, shrubs following Frank's 30-20-10 theory(1990). Also, according to species diversity index, the problem of disease and insect is researched in terms of biodiversity, and here we suggests solutions to counter such problems. According to the results, the trees planted in Suwon was found to be 31 families, 43 genus and 58 species. The most used kinds, almost 85% of the whole species, are found to be Rhododendron indicum (L.) Sweet, Buxus koreana Nakai ex Chung & al, Euonymus japonicus Thunb, Ligustrum obtusifolium Siebold & Zucc. Besides these, the rest of 15% of street trees had little variety. Therefore, it is necessary to plant tree variously and equally in terms of biodiversity. If this Frank's 10-20-30 solution is not enough to completely solve coulure problem, then further research will be done on soil properties, and local features for improvement of Suwon street tress.

• Journal of Korean Society of Forest Science
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• v.107 no.2
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• pp.151-157
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• 2018

This study was to analyze the genetic diversity of Quercus gilva Blume growing in Jeju Island for developing a preservation strategy. We examined the genetic diversity and structure using 6 ISSR primers and investigated 67 polymorphic ISSR amplicons in 80 trees distributed among five populations. The average of proportion of polymorphic loci were 93%, the average level of Shannon's information index was 0.237, and Nei's genetic diversity was 0.156. According to the analysis of the molecular variance (AMOVA), $F_{st}$ was 0.169 indicating there was a genetic variation among five populations. 17% of the total variation was allocated among the five populations, while the other 83% of the total variation was in individual trees in each population. The result could be due to the uneven number of trees among the five populations. Based on these results, the preservation strategy could be developed, for examples, considering for designation as "forest genetic resources conservation area" about the habitat, monitoring continuously, fostering the growth of seedling, ex situ preservation of genetic resources, and comparing the differences of environmental and genetic characteristic with population in ex situ.

• Korean Journal of Plant Taxonomy
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• v.43 no.4
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• pp.267-273
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• 2013

This study employed inter-simple-sequence repeat (ISSR) to assess genetic variation among 189 individuals representing 10 populations (nine in Korea and one in Japan) of Millettia japonica, which has recently been lifted from the endangered species of Korea. The calculated Shannon's information index value (I = 0.2689) of the species was appreciable and was higher than other endangered leguminous woody taxa. Gochang (I = 0.2968), Namhae (I = 0.2951), and Mt. Toham (I = 0.2823) populations showed relatively high genetic diversity, whereas the Kyushu (in Japan) population (I = 0.2487) exhibited the lowest. The results of an analysis of molecular variance indicated that 86.49% of the diversity was attributed to within populations, and 13.51% to differences among populations, suggesting that M. japonica populations do not have significant geographic differentiation and that the gene flow between populations exists to some extent (Nm = 1.8446). Continuous habitat monitoring should be conducted to conserve genetic diversity of M. japonica, particularly for those populations with relatively high genetic diversity. Selection of many individuals from the populations in Gochang, Namhae, and Mt. Toham is thought to be an appropriate strategy for ex situ conservation of M. japonica in Korea.

• Journal of Forest and Environmental Science
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• v.31 no.2
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• pp.126-135
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• 2015

This study was conducted to evaluate the ecological characteristics of forest cover types which were classified by cluster analysis in the natural forest of Sobaeksan on the basis of the vegetation data from the point-quarter sampling method. Recognized forest cover types were 1) Mixed mesophytic forest, 2) Taxus cuspidata forest, 3) Fraxinus rhynchophylla-Quercus mongolica forest, 4) Betula ermanii forest, 5) Pinus densiflora forest, 6) Quercus mongolica mixed forest, and 7) Quercus mongolica pure forest. For those of classified types, the species composition was expressed by importance value (IV) to describe the community floristically. The species diversity was quantified using the Shannon's diversity index. The results showed that the forest cover types were characteristically different from one another in growing species and compositional rates, depending upon the type which was formed by a number of similar vegetational sample points. Species diversity indices (H') of total and overstory both were the highest in the mixed mesophytic forest (3.530 and 2.880, respectively), and lowest in the Q. mongolica pure forest (2.122 and 0.000, respectively) with only one canopy species. The highest species diversity in the mixed mesophytic forest may due to the relatively high species richness and evenness in the forest cover types. The description on ecological characteristics were suggested to understand the formation and development of forest cover types in this study area.

• Journal of Ecology and Environment
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• v.36 no.3
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• pp.205-210
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• 2013

Cicuta virosa L. (Apiaceae) is a perennial emergent plant designated as an endangered species in South Korea. According to the former records, only four natural habitats remain in South Korea. A former study suggested that three of four populations (Pyeongchang: PC, Hoengseong: HS, Gunsan: GS) would be classified as different ecotypes based on their different morphological characteristics and life cycle under different environmental conditions. To evaluate this suggestion, we estimated genetic diversity in each population and distance among three populations by random amplification of polymorphic DNA. Seven random primers generated a total of 61 different banding positions, 36 (59%) of them were polymorphic. Nei's gene diversity and the Shannon diversity index increased in the order of PC < HS < GS, which is the same order of population size. In the two-dimensional (2D) plot of first two principal components in principal component analysis with the presence of 61 loci, individuals could be grouped as three populations easily (proportion of variance = 0.6125). Nei's genetic distance for the three populations showed the same tendency with the geographical distance within three populations. And it is also similar to the result of discriminant analysis with the morphological or life-cycle factors from the previous study. From the results, we concluded that three different populations of C. virosa should be classified as ecotypes based on not only morphology and phenology but genetic differences in terms of diversity and distance as well.

• The Korean Journal of Ecology
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• v.27 no.5
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• pp.257-261
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• 2004

Juniperus chinensis var. sargentii Henry is a short and creeping evergreen shrub which reaches about 50㎝ in height and occurs in the northeast Asia and in high mountains over the South Korea. Its distribution is restricted, and the number of individuals are gradually decreasing. This study was conducted to estimate spatial pattern, genetic diversity and spatial genetic structure of J. chinensis var. sargentii. A total of 131 clumps were studied in the study area (40m × 60m). The spatial pattern of this population was random (Aggregation index R=1.031). In spite of the small number and the limited distribution, the level of genetic diversity (Shannon's index 1=0.463) was relatively high as compared with those of other plant species with similar ecological characteristics. ISSR genotypes of all individuals were investigated to find the genetic relationship of clumps and genets. Fifteen clumps were composed to be clones, and a total of 116 unique genotypes were composed by separate genets. Spatial autocorrelation analysis using Tanimoto distance showed that the genetic patch was established within 8m. The effect of clonal reproduction on genetic structure was almost nothing.

• Korean Journal of Plant Resources
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• v.25 no.4
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• pp.407-415
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• 2012

Pinus pumila, which occurs in the northeast Asia, is found limitedly in Daecheongbong area of Mt. Seorak in the South Korea. This population was chosen to study spatial pattern, genetic diversity and spatial genetic structure. There were 48 polymorphic and 30 monomorphic I-SSR markers. A total of 65 individuals which distributed in the study site (40 m ${\times}$ 70 m) showed weakly aggregate distribution (Aggregate Index = 0.871). A total of 40 genets were observed from 65 individuals through I-SSR genotype comparison. Proportion of distinguishable genotype (G/N), genotype diversity (D) and genotype evenness (E) were 61.5%, 0.977 and 0.909, respectively. In spite of the small number and the limited distribution, Shannon's diversity index (I = 0.567) was relatively high as compared with those of other plant species. Spatial autocorrelation using Tanimoto's distance showed that the genetic patch was established within 12 m. Based on Mantel tests, there was relatively low correlation between genetic distance and geographic distance. Therefore, it seems the P. pumila population was formed by many parent trees in early stage. For ex situ genetic conservation of P. pumila, the sampling strategy is efficient at least above 12 m between individual trees.