• Fashion & Textile Research Journal
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• v.5 no.1
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• pp.64-70
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• 2003

Water-insoluble chitosan with molecular weight of 2,000,000, 580,000, 80,000, and 40,000 and more than 90% of degree of deacetylation were prepared to test antibacterial activity of chitosan against a pathogenic bacteria, methicillin resistant Staphylococcus aureus (MRSA). As experimental method, the Shake Flask Method (SFM) and Modified Shake Flask Method (MSFM) were applicated. The anti-microbial activity of chitosan/acetic acid aqueous solution is consistent irrespective of Mw of chitosan. MIC value of SFM measurement was 0.2 ppm, and MIC value of modified SFM measurement was 25 ppm. But MIC value of chitosan/acetic add solution and chitosan treated cotton filter paper was equally 5 ppm. The antibacterial activities of chitosan were different in different test measurements employed. The antibacterial activities of chitosan/acetic acid solution and chitosan treated cotton filter paper were also different. Therefore, it needs to be pointed out that the test measurements of anti-microbial activity have some problems.

• The International Journal of Costume Culture
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• v.2 no.1
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• pp.54-61
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• 1999

The purpose of this research was first to analyse and compare the types of microbes inherent among the unwashed and washed Kupori textiles, and second to investigate whether there is a difference between unwashed and washed Kupori textiles on the susceptibility of contamination by microorganism when exposed to the same microbial environment. Microbial identification procedure and the Shake Flask Test for investigating the effect of exposure to microbial environment were carried out separately. The result of microbial identification procedure indicated that a variety of bacteria and fungi were inherent in both unwashed and washed textiles and that the population of contaminated microorganism became more diverse after washing. The result of Shake Flask Test indicated that given the same exposure condition, the unwashed textiles tend to be more susceptible to bacterial contamination than the washed textiles. The results of the present study supported the current conservation procedures adopted in Korean museums which include washing and humigation procedures before long-term storage or display of exhumed textiles.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.33 no.3
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• pp.69-74
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• 2001

Diaper rash is found on the skin inside of babys diaper area. Most diaper rashes are caused by prolonged contact with the moisture, germs, and ammonia of the stool and urine. Some diaper rashes are caused by fungi infection such as candida albicans and Preteus vulgaris. In this study, Candida albicans was used as a test microorganism and experiment was carried out to inhibit diaper rash. Handsheets were treated with chitosan oligosaccharide, bamboo extractives, as well as mixture of monolaurin as an emulsifier and bamboo extractives. Both shake flask method and halo test were applied in order to examine the antimicrobial activity of each sample. It was found from both results of the halo test and shake flask method that handsheets treated with chitisan oligosaccharide or bamboo extractives showed antimicrobial property. In the treatment of bamboo extractives without emulsifier, no distinct difference in antimicrobial effect was found between bamboo branch and bamboo leaves extractives. In case of mixture of bamboo extractives and emulsifier, the result also suggested that bamboo branch showed better syngergistic effect than bamboo leaves. Both chitosan oligosaccharide and bamboo extractive can be used as antifungal agents against Candida albicans for making diaper.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.33 no.2
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• pp.81-86
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• 2001

The functions of food packaging are not only prevention from physical damage and loss during carrying and transportation, but also extension of shelf-life by adding antimicrobial substrate in packaging materials. Consumption of active packaging is gradually increasing. With different dosage of potassium sorbate(P.S.), the food preservative agent, antimicrobial papers were made by internal and external application of starch. The antimicrobial action of the paper was analyzed by the halo test and the shake flask method. The mechanical properties and strength were also measured. Antimicrobial papers adding P.S. showed higher values in tensile index than adding starch. The antimicrobial paper using starch showed similar microbe decreasing rate as that using P.S. Though microbe decreasing rate was 21.9%, it showed possibility to make antimicrobial paper using food preservative.

• Proceedings of the Costume Culture Conference
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• 2003.02a
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• pp.24-25
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• 2003

Water-soluble chitosan and water-insoluble chitosan with molecular weight of 2,000,000, 500,000, 80,000, and 40,000 and more than 90% degree of deacetylation were controlled to evaluate the antibacterial activity of chitosan against a pathogenic bacteria, methicillin resistant Staphylococcus aureus(MRSA), which is being issued in the world. The Shake Flask Method and Modified Shake Flask Method were used to find out the antibacterial activities of 5types of chitosan/acetic acid solution, and the other antibacterial activities test with the cotton filter treated with chitosan /acetic acid solution. Those test methods showed the great differences ,but the results of the antibacterial activites showed the same difference.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.32 no.1
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• pp.65-71
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• 2000

Chitosan oligosaccharide, chitosan polysaccharide and monolaurin were used to impart the antimicrobial properties onto paper for food products . Staphylococcus aureus (ATCC 25923) was used as test microorganism and shake flask test was carried out in order to examine the antimicrobial activity of each sample. Chitosan oligosaccaride showed the greatest antimicrobial effect among them and gave the promising result even at the concentration of 0.005%. Its antimicrobial power was enhanced when it is used with nomolaurin together. Chitosan oligosaccharide improved the tensile strength of antimicrobial paper.

• The Korean Journal of Mycology
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• v.27 no.1 s.88
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• pp.1-9
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• 1999

For the practical using of liquid spawn we carried out selection test of nutrient sources, cultural methods and cultural apparatus for liquid spawn production of oyster mushroom(ASTI 2001, ASTI 2018, ASTI 2072, ASTI 2016, ASTI 2070, ASTI 2180, ASTI 2183, ASTI 2042). The optimal temperature and pH range for mycelial growth of Pleurotus species were $25^{\circ}C\;to\;30^{\circ}C$ and 5.5 to 6.5, respectively. The effect of carbon sources, nitrogen sources and mineral salts on the mycelial growth was studied using petridish culture. Generally, the disaccharides and polysaccharides showed good effect for mycelial growth of Pleurotus species, and the polysaccharides were superior to the other classes of carbon sources for mycelial growth. For the mycelial growth of the 8 oyster mushroom stains, soybean flour was superior to the other kinds of nitrogen sources. On the other hand, addition of mineral salts did not affect, and even poor under certain mineral salts, the mycelial growth of the 8 oyster mushroom stains. The brown sugar selected out the carbon source of the agricultural medium. Also the soybean flour selected out the nitrogen source of agricultural medium. In the medium selection, we selected out agricultural optimum medium composed of brown sugar 3%, soybean flour 0.3%, potassium phosphate 0.05%, magnesium sulfate 0.05%. Under the 250 ml erlenmeyer culture, the effects of such factors as the inoculum rate, the working volume, cultural method and flask shapes on the mycelial growth were examined. The optimal inoculum rate and working volume on the mycelial growth of oyster mushroom was 2 mycelial disk (diameter 6 mm) and 100 ml, respectively. The shake flask culture was enhanced the mycelial growth than at the erlenmeyer flask. Pulp form growth of mycelium in the erlenmeyer flask culture was obtained in the culture with glass rod of length 50 mm, diameter 10 mm.

• Textile Coloration and Finishing
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• v.9 no.6
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• pp.74-78
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• 1997

This study was performed to obtain the basic data of the effect of antimicrobic finishing on chrome-tanned leather for apparel. This study was to examine the effect of conditions of antimicrobic finishing on content of antimicrobic agents in the specimen by analysis and on the antimicrobic activity of specimens by means of shake flask test. The optimum conditions of antimicrobic finishing were found to be ; the concentration of Si-QAC-0.5%(o.w.f.), treating time-30minute, and drying method-air drying.

• Textile Coloration and Finishing
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• v.6 no.4
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• pp.1-8
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• 1994

To improve the antimicrobial activity and crease resistance of Korean traditional Hansan ramie fabrics, partially saponified polyethylene(PPS) was used as a softening water repellent agent and 3-(trimethoxysilyl)propyl dimethyl octadecyl ammonium chloride(QAC) as a antimicrobial agent. The antimicrobial activity was effective by treatments of PPS, QAC and PPS/QAC for shake flask test. For the halo test, PPS/QAC showed good antimicrobial activity. It was found that the desirable composition of H$_{2}$O/PPS/QAC is 98/1/1 for improving the antimicrobial activity and crease resistance of the fabrics.

• Textile Coloration and Finishing
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• v.5 no.1
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• pp.33-39
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• 1993

This study was to examine the anti-bacteria effect of chrome-tanned garment leathers by various processes to article from hide. Anti-bacterial test by halo test and shake flask method, and pH value measurment are carried out in this study. The results can be obtained as follows: 1. In the soaking process of chrome-tanned garment leathers, it needs more effective anti-bacterial treatment. 2. pH value of samples by various processes has affected to anti-bacteria effect. 3. Chrome used by the tanning and the neutralization process have been found to be the anti-bacteria activity. 4. The dyeing, the fatliquoring and the finishing process has required anti bacteria treatment.