• Korean Journal of Environment and Ecology
• /
• v.28 no.5
• /
• pp.523-530
• /
• 2014

One juvenile raptor which was not able to be identified due to its head damage was discovered on a roadside in Janggok-ri, Jori-eup, Paju on 28th June, 2011. The species was identified by DNA barcoding. After polymerase chain reaction (PCR) of the mitochondrial cytochrome c oxidase subunit I gene (COI), we obtained 695 bp sequences. We analyzed the obtained COI sequence with similar sequences from the BOLD systems and BLAST of the NCBI Genbank, and discovered that its sequence showed 100 % similarity values with the one of the five gray-faced buzzards which were previously researched. In addition, it was confirmed to be a female through sex determination using DNA. Such results are important information as it confirms the breeding of the gray-faced buzzards for the first time in 43 years since its breeding was last recorded in 1968, in Paju. Wildlife rescue center needs to work with adjacent consigned registration and preservation institutions when carcass of wild animals is collected or DNA samples are obtained for more accurate both species and sex identification through a systematic management system in the future. Furthermore, the obtained DNA sample of the gray-faced buzzard and COI gene, DNA barcode, could be used as reference standards for similar researches in the future.

• Korean Journal of Poultry Science
• /
• v.46 no.4
• /
• pp.287-296
• /
• 2019

Avian sex determination system involves the male ZZ and female ZW chromosomes. However, very few studies are reported the expression, functional role and importance of genes on the W chromosome because of its small and highly heterochromatic genomic regions. Recent studies demonstrated that the W chromosome may have critical roles in physiology, sex determination and subsequent sexual differentiation in chickens. Therefore, gene annotation, including describing the expression and function of genes in the chicken W chromosome, is needed. In this study, we have searched the W chromosome of chickens and selected a total of 36 genes to evaluated their specific expression in the testis and ovary at various developmental stages such as embryonic day 6 (E6), hatch and adult. Interestingly, out of 36 genes in chicken W chromosome, we have found seven female-specific expression at E6.5 day, indicating that they are functionally related to female chicken gonadal differentiation. In addition, we have identified the stage specific gene expression from the sex specific genes. Furthermore, we analyzed the relative location of genes in the chicken W chromosome. Collectively, these results will contribute molecular insights into the sexual determination, differentiation and female development based on the W chromosome.

• Korean Journal of Environmental Biology
• /
• v.41 no.4
• /
• pp.557-569
• /
• 2023

Amphibians are the world's most threatened group of animals, with approximately 41% facing extinction. Contrary to this global trend, the number of amphibian species in Korea has increased by approximately 53.3% over the past 20 years. In particular, salamanders within the genus Hynobius showed even greater diversity, with the number increasing three-fold from two species to seven. However, morphological and ecological traits required for the clear differentiation of these animals are yet to be determined, leading to much confusion. In this study, we investigated the validity of using size traits for species identification, as this method is currently considered a rule of thumb when differentiating Geoje salamanders (Hynobius geojeensis), cryptic Uiryeong salamanders (H. perplicatus), and Korean small salamanders (H. unisacculus). Our study revealed that sex, study period, and habitat were all factors associated with significant differences in snout-vent length, head width, and body weight. Differences in these size traits were evident both between and within species. Our results show that body size traits applied in the recent classification of the three new salamander species could not be seen as a suitable criterion. Such identification methods based solely on body size not only cause great confusion in the field but will also limit future research on Korean Hynobius salamanders.

• The korean journal of orthodontics
• /
• v.32 no.2 s.91
• /
• pp.79-89
• /
• 2002

The purpose of this study is to evaluate the reproducibility and errors in landmark identification of conventional lateral cephalometric radiography and digital lateral cephalometric radiography. Fifteen conventional lateral cephalometric radiographs and fifteen digital lateral cephalometric radiographs were selected in adults with no considerations on sex and craniofacial forms. Each landmark was identified and expressed as the coordinate (x, y). The landmarks were classified into 3 groups. The landmarks of the first identification was T1, identification after one week was T2, and identification after one month was T3. The mean and standard deviation of identification errors between replicates were calculated according to the x and y coordinates. The errors between first identification and second identification were expressed as T2-T1(x), T2-T1(y) and those between first identification and third identification were expressed as T3-T1(x), T2-T1(y). Each was divided into conventional lateral cephalometric radiography and digital lateral cephalometric radiography. The independent t- test was used for statistical analysis of identification errors for the evaluation of reproducibility. The results of this study were as follows ; 1. Generally, the mean and standard deviation of landmark identification errors in digital lateral cephalometric radiography was smaller than those of conventional lateral cephalometric radiography. 2. Only a few landmarks showed statistically significant difference in identification error between conventional lateral cephalometric radiography and digital lateral cephalometric radiography. 3. The enhancement of image quality didn't guarantee decrease in landmark identification error and didn't affect tendency of landmark identification error.

• Journal of Agricultural Extension & Community Development
• /
• v.13 no.1
• /
• pp.29-47
• /
• 2006

The objectives of this study were to explore rural elderly's perception on rural area as a proper place to live for the elders and to find out their residential satisfaction and its related variables. The data were gathered through the questionnaire survey from 241 rural elderly over 70 aged living in Gyeonggi province during July 2005. The major findings of this study were as follows: 1) Those who are men, having experience living in urban area, and favorable perception to social relation with other residences prefer the rural area to urban area as living place for the elderly ; 2) Those who are subjectively perceived healthy and favorable perception to social relation with other residences tend to be in higher residential satisfaction than other groups ; 3) Such variables as age, educational background, living term in community and urban area were not significant to rural elderly's perception on rural area as a proper place to live for the elders and residential satisfaction; 4) Such variables as identification as community residences, people-oriented personality, sex and having experience living in urban area explained 26.4% of variance in rural elderly's perception on rural area as a proper place to live for the elders ; 5) Such three variables as identification as community residences, relationship with the primary group and age explained 55.2% of variances in residential satisfaction; 6) In shortly, the most influential factor to rural elderly's perception on rural area as a proper place to live for the elder and residential satisfaction was their identification as community residences.

• Fisheries and Aquatic Sciences
• /
• v.20 no.11
• /
• pp.29.1-29.7
• /
• 2017

The objective of this study was to determine a simple and reliable ploidy identification protocol for the rainbow trout (RT), Oncorhynchus mykiss, in the field condition. To evaluate the ploidy level and compare different detection protocols, triploid RT and gynogenesis were induced by UV irradiation and/or heat shock. The hatching rate at day 30 was 85.2% and the survival rate at day 90 was 69.4% (fingerling). The sex ratio of female RT was 93.75% in the gynogenesis group, illustrating that the UV irradiation inactivated the sperm DNA. The hatching rate and survival rate were 82.0 and 74.7%, respectively, in the triploid-induced group. The triploid induction rate by heat shock procedure was 73.9%. Cytogenetic protocols for ploidy identification such as chromosome counting, erythrocyte nuclear size comparison, and analysis of nucleolar organizing regions (NORs) by silver staining were compared. Silver nitrate staining showed the greatest success rate (22/23 and 32/32 for the triploid-induced group and gynogenesis group, respectively), followed by erythrocyte nuclear size comparison (16/23 and 19/32 for the triploid-induced group and gynogenesis group, respectively) and, lastly, chromosome preparation (2/23 and 6/32 for the triploid-induced group and gynogenesis group, respectively) with the lowest success rate. Based on our findings, silver staining for RT ploidy identification is speculated to be highly applicable in a wide range of research conditions, due to its cost-effectiveness and simplicity compared to other numerous ploidy detection protocols.

• The KIPS Transactions:PartB
• /
• v.12B no.2 s.98
• /
• pp.123-130
• /
• 2005

In this paper, fingerprint identification algorithm using pixel direction factor in blocks is proposed to minimize false acceptance ratio and to apply security system. The proposed algorithm is that a fingerprint image is divided by 16 blocks, then feature parameters which have direct factors of $0^{\circ},\;45^{\circ},\;90^{\circ}\;and\;135^{\circ}$ is extracted for each block. Membership function of a reference fingerprint and an input fingerprint for the extracted parameters is calculated, then identification of two fingerprint is distinguished using fuzzy inference. False acceptance ratio is evaluated about different fingerprints of In kinds regardless of sex and shape which are obtained from adults, and false rejection ratio is evaluated about fingerprints which are obtained by adding fingerprints of 10 kinds on different fingerprints of 100 kinds. The experiment results is that false acceptance ratio is average $0.34\%$ about experiment of 4,950 times, and false rejection ratio is average $3.7\%$ about experiment of 1,000 times. The proposed algerian is excellent for recognition rate and security.

• The Research Journal of the Costume Culture
• /
• v.7 no.2
• /
• pp.323-333
• /
• 1999

The purpose of this study was to analyze the meaning and formative features of the black color reflected on the fetish fashion. This study was proceeded mainly by analyzing with references and plates of magazine. The results of the study were as follows ; First, the sexual liberation and revolution have certainly led to a reassessment of sexual deviations, which changed the meaning of fetishism that has been only regarded as sexual perversion, so that it symbolied a new sexual perception and identification. As the result, black in fetish fashion was symbolized a new fetishism\`s definition which refused to distinguish male from female, and also reflected the meaning to express the second sex that wanted to express themselves. Second, the formative features of black color in fetish fashion have appeared tightness and bareness in form, and in material, glancing materials changed by the effect of light, and elasticity materials-leather, rubber, PVC, and laycra. By these features, black fetish fashion has drawn a higher attention on human body than the dress itself as a symbol about the psychology of erotic sensations and sexual images of the self appearing in black color.

• Journal of Korean Public Health Nursing
• /
• v.22 no.2
• /
• pp.165-176
• /
• 2008

Purpose: This study was conducted in order to analyze the factors that influence alcohol use disorder in elders in KyongBuk province. The results of the study will provide basic information for the development of nursing interventions to promote proper alcohol use in elders. Methods: The subjects were 626 elderly individuals. Alcohol use disorder was defined as a score of more than 15 point on the AUDIT-K(Korean version of Alcohol Use Identification Test). Descriptive statistics, chi-square test, ANOVA and logistic regression were utilized to analyze the data. Results: Overall incidence of alcohol use disorder level; normal 73.2%, problem drinking 7.5% and alcohol use disorder 19.3%. Significant factors affecting alcohol use disorder were sex (OR=6.897), religious belief (OR=1.836), smoking (OR=2.948), liver disease (OR=4.753) and depression (OR=1.779). Conclusion: Community health care nurses perform a crucial function in the screening of elderly alcoholics. Early detection and treatment of depression in elders may help to prevent alcoholism.

• Sleep Medicine and Psychophysiology
• /
• v.19 no.1
• /
• pp.18-21
• /
• 2012

Parasomnias induced by hypnosedatives are rare but serious side effect. Such parasomnias have not been reported with all hypnosedatives. However, frequent use of hypnosedatives, particularly nonbenzodiazepine receptor agonists is associated with parasomnias. Associated symptoms are sleep eating, sleepwalking with object manipulation, sleep conversations, sleep driving, sleep sex and sleep shopping etc. Mechanisms include high affinity for $GABA_A$ receptor, interruption of the consolidation phase of memory formation by drug, pharmacokinetic or pharmacodynamic drug-drug interaction and concomitant administration with alcohol. Managements for parasomnias induced by hypnosedatives involve stopping medication, switch to other medications or nonpharmacological treatment, lowest effective dose of NBRAs (Non-Benzodiazepine Receptor Agonists), taking into consideration drug-drug interactions, identification and treatment of underlying disease states.