• 한국가축번식학회지
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• 제20권2호
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• pp.89-99
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• 1996

The purpose of this study was to develop the diagnosis techniques for sex determination of rabbit embryos at preimplantation stage. To detect male specific sequences using polymerase chain reaction, two genes functional on sex determination including SRY and ZFX/Y genes were targeted using multiple oligonucleotide primer sets. Three of them for conserved SRY gene were used for appropriate amplification pattern, and then only one primer set #3 proved to be most efficient, showing male-specific strong signal ofamplified sequences. Using this male specific bandsfrom human, cattle, pig and mouse, the gender of rabbit was determined. As an another system for sex determination system, amplified 910bp fragment from ZFX/Y was digested with several restriction endonuclease and showed gender specific restriction fragments only by Hinf I. Using two different system for sex identification of rabbit in this study, blind tests for 17 samples was conducted and showed identical results from two different methods. And then, amplification limit of PCR reaction for template DNA was estimated using various amounts of DNA for both SRY and ZFX/Y systems, resulted as 20pg and 800pg, respectively. With this results, test for gender identification of rabbit embryos were performed using SRY derived amplification system. From total 22 embryos selected for its developmental state 18 were identified as male embryos, showing significant difference from expected sex ratio 1:1. This biased sex ratio was interpreted as to have been caused by the fact, reported by the fact, reported by several researchers, that male embryos develop more rapidly and are more resistant against the in vitro manipulation than female embryos.

• 한국발생생물학회지:발생과생식
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• 제14권1호
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• pp.7-11
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• 2010

This study attempted to verify the possibility of using germ cell aspiration (GCA) method as a non-fatal technique in studying the life-history of equilateral venus, Gomphina veneriformis (Veneridae) and granular ark, Tegillarca granosa (Arcidae). Using twenty-six gauge 1/2" (12.7mm) needle, GCA was carried out in equilateral venus through external ligament. In granular ark, GCA was performed by preventing closure of the shells by inserting a tongue depressor between the shells while still open. The success rate of sex identification using the GCA method was 95.6% for the equilateral venus (n=650/680) and 94.3% for the granular ark (n=707/750). Mortality of equilateral venus, which spent 33 days under wild conditions, was 13.8% (n=90/650) while the mortality of granular ark, which spent 390 days under wild conditions, was 2.4% (n=17/707). Although we believe that GCA does not appear to cause death in equilateral venus or granular ark, the success rate in employing of this methodology may differ depending on the level of proficiency of the researcher and reproductive stage of the bivalve. This study concludes that GCA is a convenient non-fatal methodology, which can be employed to identify sex and investigate gonadal maturity in Gomphina veneriformis and Tegillarca granosa.

• Asian-Australasian Journal of Animal Sciences
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• 제15권11호
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• pp.1531-1535
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• 2002

It has been known that the sex of chicken cells can be most accurately identified by fluorescence in situ hybridization (FISH). However, the presently available FISH has not been widely used for sex identification, because the procedures for cell preparation and FISH itself are complicated and time-consuming. The present study was undertaken to test a rapid FISH procedure for sexing chicken. A FISH probe was simultaneously synthesized and labeled with digoxigenin by polymerase chain reaction (PCR) targeting a 416 bp segment of the 717 bp XhoI family fragment which is repeated over 10 thousand times exclusively in the W chromosome. Sexing by FISH was performed on cytological preparations of early embryos, adult lymphocytes and feather pulps of newly hatched chicks. The DNA probe hybridized to all types of uncultured interphase as well as metaphase female but not male cells that had been examined. Moreover, consistent with the known site of the XhoI family, the hybridization signal was localized to the pericentromeric region of the W chromosome. We, therefore, conclude that the present PCR-based FISH can be used as a rapid and reliable sex identification procedure for chicken.

• Toxicological Research
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• 제35권4호
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• pp.331-341
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• 2019

Cancer is one of the common causes of death with a high degree of mortality, worldwide. In many types of cancers, if not all, sex-biased disparities have been observed. In these cancers, an individual's sex has been shown to be one of the crucial factors underlying the incidence and mortality of cancer. Accumulating evidence suggests that differentially expressed genes and proteins may contribute to sex-biased differences in male and female cancers. Therefore, identification of these molecular differences is important for early diagnosis of cancer, prediction of cancer prognosis, and determination of response to specific therapies. In the present review, we summarize the differentially expressed genes and proteins in several cancers including bladder, colorectal, liver, lung, and nonsmall cell lung cancers as well as renal clear cell carcinoma, and head and neck squamous cell carcinoma. The sex-biased molecular differences were identified via proteomics, genomics, and big data analysis. The identified molecules represent potential candidates as sex-specific cancer biomarkers. Our study provides molecular insights into the impact of sex on cancers, suggesting strategies for sex-biased therapy against certain types of cancers.

• Bulletin of the Korean Chemical Society
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• 제16권8호
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• pp.688-689
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• 1995

• 생명과학회지
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• 제21권5호
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• pp.626-630
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• 2011

세계적 멸종위기종인 따오기(Nipponia nippon)는 2008년 10월에 중국에서 1쌍이 도입된 후 한국최초로 인공번식에 성공하였다. 본 연구는 따오기의 sex-related gene과 Chromodomain Helicase DNA Binding Protein gene (CHD gene)을 가지고 polymerase chain reaction (PCR)을 수행하여 새로 태어난 따오기 유조의 성별을 확인하고자 하였다. 본 연구에서는 따오기의 성별 확인을 위해 PCR후 제한효소의 처리 방법과 P2과 P8를 이용한 PCR 방법을 실시하였을 때 더 정확한 결과가 나타남을 알 수 있었다. 그리고 CHD gene의 염기서열을 선행연구와 비교해 본 결과, 암컷의 염기서열에서 1~2 base pairs 차이가 나타남을 알 수 있었다.

• 대한물리의학회지
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• 제5권3호
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• pp.421-434
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• 2010

Purpose : The purpose of this study is to understand the attitudes to internal service quality between coworkers, service quality to patients, job satisfaction, organizational identification and job identification of physical therapists in medical organizations. Methods : Data were collected from physical therapists working in medical organizations. 195 questionnaires were analyzed. Participants' total mean, group mean by age, sex, career and type of medical organization and the variables' correlations were examined. Results : The attitudes of physical therapists to five variables were very positive. Especially job identification was strong. The significant differences between groups were found in some variables. All variables except age and career were positively related to each other. The correlation between age and career was very high. Age and career were related to some of variables. Conclusion : To improve organizational performances it should be a top priority to understand the attitudes of physical therapists in organizations.

• Journal of Oral Medicine and Pain
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• 제20권1호
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• pp.229-246
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• 1995

The deoxyribonucleic acid(DNA) was isolated from the pulp, dentin and enamel of the 4 fresh teeth and the 7 teeth left in room temperature for 10 years. Then it was examed to find out the usefulness for forensic dental medicine. Samples of the tooth-derived DNA amplified by polymerase chain reaction(PCR), electrophosed for sex determination by detection of X-Y homologous amelogenin gene and D1S80 locus detection. The following results have been achieved. DNA extraction was possible in pulp and dentin of the fresh teeth, so it could be applicatable to detection of X-Y homologous amelogenin gene for sex determination, amplification of D1S80 locus by PCR. Sex determination was possible in pulp and dentin of the teeth left at room temperature for 10 years. Also, possible the detection fo AMP-FLPs to increase PCR cycling up to 40. DNA was isolated from all pulp of the fresh teeth and the teeth left in room temperature for 10 years, and also isolated from the dentin of the fresh teeth, partially isolated(3/7) from the dentin of the teeth left in room temperature for 10 years, but DNA was not isolated from enamel. From the above investigation, DNA extraction, sex determination, amplification of D1S80 locus were successfully accomplished even though the teeth were left for 10 years at room temperature. Therefore, teeth, especially pulp, are highly reliable and applicable as molecular biological samples for individual identification.

• 복식
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• 제49권
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• pp.155-172
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• 1999

The purpose of this study was to investigate the effect of children's personality traits and parental child-rearing attitudes on clothing attitudes and color evaluation of preschool children. The subjects for this study consisted of 360 five-year-old children who were attending a preschool and their parents in Cheju Island Korea. Employed mothers had significantly higher score on love acceptance attitude than unemployed mothers did. Female children had more conservative attitude on clothing sex-role than male children did There were no significant gender differences in parental child-rearing attitudes. There were significant relationships between gender and preferred clothing colors. Male children were more likely to prefer blue red, green, and prussian blue for the colors of clothing than were female children while female children liked pink yellow and orange more than male children. Boys selected blue and girls selected pink as the most preferred clothing color. Overall preschool children chose red as the most preferred color black as the most disliked color. The children's sex identification by clothing color was highly consistent with the conservative attitude of adults for all colors. That is preschool children identified pink red and yellow with female color while prussian blue blue and green with male color. Clothing interest of boys was influenced by the mother's state of employment and academic trait. The boys high in academic trait and whose mothers did not have job were high in clothing interest. Clothing management of boys was influenced by emotionality and love acceptance rearing Ttitude. Clothing independednce of boys was influenced by authority control rearing attitude Clothing sex-role of boys was influenced by familism trait. The boys high in familism trait had open-hearted attitude in clothing sex-role. Clothing interest of girls was influenced by academic trait clothing management by sociality clothing independence by emotionality and clothing satisfaction by familism trait. Therefore the more positive personality children had the higher clothing interest and the more desirable clothing attitudes they had.

• 복식문화연구
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• 제2권1호
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• pp.55-76
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• 1994

The purpose of this study was to analyze the classification and meaning of symbols, of the clothes of the college students by sex-roll theory and identity theory. The clothes analyzed in this study were collected by photographs which were taken in the campus (240 out of 1,000 pictures) of the several colleges and universities in Seoul form fall in 1993 to summer in 1994. The results were as follow : 1. Analysis as a symbol of sexuality. The phenomenon of the visual inconsistency and consistency of sexual image in dress were showed simultaneously. The clothes of male students were generally becoming feminine style in materials and colors of clothes. These suggested that sex-roll theory be applied to their clothes. 2. Analysis as symbols of identification or individuality. The identification of shoes, bags, accessories, and hair styles were prominent than that of clothes. When it was analyzed as a symbol of individuality, the college students seemed to act as fashion leaders, who accepted new fashions and tried them on first. These suggested that Erikson′s theory on identity be applied to their clothes. 3. Analysis as a symbol of emblem. The dissimilarities of between the college students and other groups in the same generation were bright and casual attire with files, books, and sack. 4. Analysis as a symbol of campus ceremony. The clothes of college students on campus ceremonies were more causal and flexible than those of other groups in the same generation. It was known that the symbols showed above were reflected on their clothes as "one′s expressions" which are sex-roll, identity, and characteristics of college students.