This study aimed to select a yeast strain for optimizing the quality of distilled spirits. The brewing and distilling properties of 4 KFRI (Korea Food Research Institute) yeasts (Y88-4, Y98-4, Y172-6, Y192-4) and 2 industry yeasts (C1, C2) were compared. For investigating the possibility of using these strains on an industrial scale, diverse analytical methods were applied to assess parameters associated with distilled spirit quality such as alcohol content, pH, total acidity, and soluble solid content. After 11 days of fermentation, the alcohol strength obtained using six yeast strains reached 13.9-16.4% (v/v), while pH was 3.9-4.0, and total acid was 0.40-0.52%. To compare GC-MSD Volatile flavor components, all the distilled spirit samples were diluted to 20% (v/v) alcohol strength. Seven fusel alcohols, 26 esters, 2 acids, and 3 miscellaneous compounds were detected in the distilled spirits. Y88-4 had the most abundant volatile flavor component and scored the highest overall preference in sensory evaluation. After analyzing the various properties of yeasts, strain Y88-4 was finally selected as the best strain for producing distilled spirits.
The cultivation methods are important for determining crop yield of green manure. The effect of cultivation methods of green manure crops (GMC) on biomass and rice yield was investigated. This experiment was conducted at Sinheung series (fine loamy, mixed, nonacid, mesic family of Fluvaquentic Endoaquepts from Oct. 2007 to Oct. 2008 at the National Institute of Crop Science (NICS), RDA, Suwon, Gyeonggi province, Korea. Seven GMC (hairy vetch, barley, Chinese milk vetch, rye, crimson clover, oats, rattail fescue) were cultivated and incorporated on paddy soil by broadcasting before rice harvesting (BBRH) and partial tillage seeding (PTS). Among the three leguminous GMC, the biomass and N production were the highest at the hairy vetch of PTS. Among the four gramineae GMC, the biomass and N production tended to be higher in the rye of BBRH and barley of PTS. The C/N ratio (56.5~74.2) of rye was high compared with hairy vetch (14.1). Among the GMC, the incorporation of hairy vetch increased $NH_4$-N contents in rice paddy soil at 14 and 42 days after transplanting. These results showed that hairy vetch had no significant to rice yield compared with conventional fertilization. Therefore, hairy vetch seems to be the most efficient green manure crop as an alternatives to chemical N fertilizer in the central regions of Korea.
The satellite-viewed cloudiness, a ratio of cloudy pixels to total pixels ($C_{sat,\;prev}$), inevitably differs from the "ground-viewed" cloudiness ($C_{grd}$) due to different viewpoints. Here we develop an algorithm to retrieve the satellite-viewed, but adjusted cloudiness to $C_{grd} (C_{sat,\;adj})$. The key process of the algorithm is to convert the cloudiness projected on the plane surface into the cloudiness on the celestial hemisphere from the observer. For this conversion, the supplementary satellite retrievals such as cloud detection and cloud top pressure are used as they provide locations of cloudy pixels and cloud base height information, respectively. The algorithm is tested for Himawari-8 level 1B data. The $C_{sat,\;adj}$ and $C_{sat,\;prev}$ are retrieved and validated with $C_{grd}$ of SYNOP station over Korea (22 stations) and China (724 stations) during only daytime for the first seven days of every month from July 2016 to June 2017. As results, the mean error of $C_{sat,\;adj}$ (0.61) is less that than that of $C_{sat,\;prev}$ (1.01). The percent of detection for 'Cloudy' scenario of $C_{sat,\;adj}$ (73%) is higher than that of $C_{sat,\;prev}$ (60%) The percent of correction, the accuracy, of $C_{sat,\;adj}$ is 61%, while that of $C_{sat,\;prev}$ is 55% for all seasons. For the December-January-February period when cloudy pixels are readily overestimated, the proportion of correction of $C_{sat,\;adj$ is 60%, while that of $C_{sat,\;prev}$ is 56%. Therefore, we conclude that the present algorithm can effectively get the satellite cloudiness near to the ground-viewed cloudiness.
Yang, Sungmin;Choi, Hyo Won;Kang, Yun Koo;Lee, Jin-Sung;Namgoong, Mee Kyung
Journal of The Korean Society of Inherited Metabolic disease
/
v.20
no.2
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pp.55-62
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2020
A 22-month-old girl who had taken iron supplements due to iron deficiency anemia, presented bloody mucoid stool for one month. She had a bruise at the right periorbital area due to minor trauma and hepatosplenomegaly. Laboratory studies showed anemia, thrombocytopenia, elevated alkaline phosphatase (ALP), hypophosphatemia, decreased haptoglobin, hypocomplementemia, negative direct/indirect Coomb's test, normal vitamin D3 level and high PTHi. Wrist x-ray showed no signs of rickets. The abdominal ultrasound showed only accessory spleen. Tandem mass spectrometry was normal. During follow up, bloody stool regressed after seven days of withdrawal of iron supplement and cow milk, and the total CO2 level had been within 15-20 mEq/L with normal anion gap. NGS (next generation sequencing) panel test for evaluation of renal tubular acidosis showed negative results. After low dose steroid and vitamin D supplements under the impression of hypocomplementemic vasculitis, thrombocytopenia, C3/C4, decreased haptoglobin, and elevated ALP level became normal. At 57 months of age, laboratory findings showed elevated liver enzyme, ALP and gamma-glutamyl transferase again. And liver cirrhosis with splenomegaly and diffuse renal disease were reported with abdomen CT scan. Liver biopsy reported macro- and micronodular cirrhosis. Urine organic acid profile showed elevated succinylacetone level. Whole exome sequencing revealed novel compound heterozygous mutations (NM_00137.2:c.107T>C, NM_00137, 2:c.614T>C) in FAH gene and confirmed by Sanger sequencing. Consequently, the patient was diagnosed as chronic hereditary tyrosinemia type I. She started low phenylalanine/tyrosine diet and nitisinone treatment. Our case had presented symptoms very slowly, which is the first case of chronic tyrosinemia type I in South Korea.
Kim Sol-A;Lee, Jeong-Eun;Park, Hyun-Jin;Park, Mi-Seon;Choi, Song Yi;Shim, Won-Bo
Journal of Food Hygiene and Safety
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v.36
no.6
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pp.493-503
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2021
The study was designed to analyze raw and auxiliary materials of Korean traditional cookies such as Yugwa and Gangjeong, equipment and tools, personal hygiene of workers and microbial contamination of materials by each manufacturing process. In addition, it looked at washing method for reducing microorganisms at the site and reduction effect of microorganisms by frequency in the manufacturing processes of Yugwa. In the process of producing Korean traditional cookies, the level of total aerobic bacteria (TAB) in popped rice was 1.2 Log CFU/g and the level of TAB in finished products increased to 3.7 Log CFU/g. In the process of producing Yugwa, the level of TAB increased to a maximum of 6.5 Log CFU/g in the soaking process but decreased to 1.3 Log CFU/g in the frying process. However, the level of TAB increased again to 1.3 Log CFU/g in finished products that proves its recontamination. It is estimated that he manufacturing process causes cross-contamination that comes from the work tools, equipment or workers. In particular, the spatula, one of the work tools, was found to have 4.4 Log CFU/g of aerobic bacteria and 4.2 Log CFU/g of colon bacillus that show they are highly contaminated. In the soaking process of Yugwa that lasts seven days, the level of TAB was a maximum of 10 Log CFU/g and the level of total colon bacillus was 6.8 Log CFU/g. When compared with washing methods, using hands and tools or running water, it is confirmed that the level of both TAB and total colon bacillus decreased to 5.0 Log CFU/g and 2.8 Log CFU/g respectively when hands were washed with running water 10 times. The above result shows that it's required for workers to wash their hands as well as wash and disinfect work tools and equipment in the process of producing Korean traditional cookies at small-scale companies. In addition, to reduce the level of microbial contamination in finished products, workers are required to apply their reduction method at the site.
The heading responses of rice varieties that originated from South Korea, North Korea, and northern China were examined under the temperature and day-length conditions of 13 major rice production areas in North Korea. Kenjiandao3 and Nongdae3 originated from China, Olbyeo1, Olbyeo2 and Sonbong9 from North Korea, and Joun from South Korea demonstrated the earliest heading stage depending on the regional environment. Out of 40 rice varieties, 34 reached the heading stage within the regional safe marginal heading date (SMHD) under Haeju and Sariwon environmental conditions, while 16 to 17 varieties reached the heading stage under Wonsan, Changjon, Supung, and Yongyon environmental conditions. Some middle and mid-late maturing varieties that originated from South Korea reached the heading stage within the SMHD under the temperature and day-length conditions of Kaesong, Haeju, Sariwon, Nampo, and Pyongyang that are located in the west-southern plain. The majority of early maturing varieties, but not the middle or mid-late ones, reached the heading stage within the SMHD under the environmental conditions of Singye, Anju, Kusong, and Sinuiju. Only a few early maturing varieties demonstrated the heading stage within the SMHD under Yongyon, Changjon, and Wonsan environments. The number of days to heading was highly positively correlated among all regions; however, it was not consistent among the rice varieties. The 40 rice varieties that had been tested were classified into seven groups according to their heading responses to the temperature and day-length variations of the 13 regional conditions at 65% similarity level in cluster analysis.
The 20th National Congress of the Chinese Communist Party (hereafter referred to as "the 20th National Congress") was under the global spotlight long before it was held for seven days from 16 to 22 October 2022. People wondered whether Xi Jinping would secure a third term as China's leader or whether he would lay the foundations to be in power forever during the third term. In Korea, the press and media questioned whether the event would become the "crowning of Emperor Xi (Xi Huangdi)," whose power rivaled that of the first emperor in China, Shi Hunagdi, and featured the scene where Hu Jintao was forced to leave the venue during the Congress. On the other hand, many Korean academics focused more on how Xi would organize the Politburo and its Standing Committee and whether the outline of his heirs would appear during the event. This tendency in academia in turn worsened the media's concerns. This paper presents a quantitative analysis of the 20th National Congress Report, as opposed to an analysis of Xi's political intentions at the event. The National Congress Report outlines the Party's visions, goals, and strategies for the next five years in politics, economy, society, culture, foreign affairs, and relationship with Taiwan. The authoritative document is rich in narrative and logic and deserves academic study. This research analyzes the 18th, 19th, and 20th Reports by identifying their keywords and regular expressions and checking their frequency and percentage through text-mining methods. This approach enables the quantification and visualization of the significant changes in the Party's sovereign vision over the fifteen years of Xi's rule from 2013 to 2027.
Woo-Do Lee;Jiseon Son;Hyun-Soo Kim;Hee-Jin Kim;Yeon-Seo Yun;Hwan Ku Kang;Woncheoul Park;Han Ha Chai;Eui-Chul Hong
Korean Journal of Poultry Science
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v.50
no.2
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pp.101-108
/
2023
This study was conducted to investigate the appropriate levels of crude protein (CP) and phytase in the diet of broiler chicks in order to reduce nitrogen and phosphorus contents in feces while maintaining performance of broilers. Six hundred forty-eight 1-day-old male broilers (41.9±0.91 g) had a total of 3 × 3 complex factor of 3 levels of CP (22%, 21%, 20%) and 3 levels of phytase (1,000, 800, 500 FTU/kg) in the diet. Divided into 9 treatments, 4 replications per treatment, 18 birds per replication, were completely randomly assigned and reared in a metabolic cage for 7 days. Seven-day-old body weight (BW) and body weight gain (BWG) of broilers were significantly lower at CP 20% treatment (P<0.05), and feed conversion ratio (FCR) was significantly lower at CP 21% and phytase 800 FTU/kg treatment (P<0.05). Nitrogen and phosphorus contents in chicken excreta were significantly lower in CP 20% and phytase 500 FTU/kg treatment, respectively (P<0.05). Interactions between CP and phytase in the feed were shown for nitrogen and phosphorus in feces (P<0.05). In conclusion, considering the broiler performance and excretion contents of nitrogen and phosphorus, it is thought that CP and phytase levels of broiler chicks diet can be reduced by 21% and 800 FTU/kg, respectively.
This study investigated the effects of three music genres (classical, new age, and rock) on the stress levels of six Jeju crossbred horses (Jeju horse × Thoroughbred) in a horse stable. The horses were exposed to the three genres for seven days, and their stress levels were measured by analyzing physiological markers, including neurotransmitter (cortisol, β-endorphin, dopamine, serotonin, and oxytocin) plasma levels and creatine phosphokinase (CPK) and aldolase serum levels. The neurotransmitter analysis showed significant differences in cortisol levels between classical and new age music exposure. Dopamine levels decreased significantly only with new age exposure. Although there were no significant differences in β-endorphin levels between the three genres, β-endorphin levels decreased with increasing classical and new age music playback times and increased with increasing rock music playback times. There were no significant differences in serotonin levels between the three genres. Oxytocin levels decreased significantly with exposure to classical and rock music. The CPK and aldolase analyses showed that CPK levels decreased significantly only with exposure to new age music and increased after playback ended, while aldolase levels decreased significantly with classical and new age music exposure and increased after playback ended. These findings suggest that classical music and new age are the optimal music genres for the psychological stability of Jeju crossbred horses. Playing back an appropriate music genre could be used to improve breeding and promote the welfare of Jeju crossbred horses.
Journal of Practical Agriculture & Fisheries Research
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v.25
no.4
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pp.138-147
/
2024
Agrobacterium-mediated transformation (AMT) is a method that allows for the stable integration of DNA fragments into the plant genome. Transgenic plants generated through AMT typically exhibit a lower copy number of the transgene compared to those induced by particle bombardment. Furthermore, AMT offers a straightforward and efficient approach for generating transgenic plants. While the transformation efficiency of wheat is comparatively lower than that of other monocot plants such as Rice (Oryza sativa L.) and Maize (Zea mays L.), the cultivars 'Bobwhites' and 'Fielder' are commonly employed for wheat transformation. To date, there have been no reported instances of successful development of transgenic plants using Korean wheat varieties through AMT. This study aims to assess the transformation efficiency of 43 Korean wheat cultivars using the GUS assay, with the goal of identifying suitable Korean wheat cultivars for AMT. The pCAMBIA1301 vector, carrying the β-glucuronidase (GUS) gene, was incorporated into Agrobacterium strain EH105. Following the inoculation of Agrobacterium into immature embryos, GUS assays were conducted 'Saeol', 'Jopum', and 'Jonong' showed 100% (the number of embryos showing GUS spots/the number of embryos used for AMT) among 43 cultivars. In addition, cultivars with more than 70% were 'Saekeumgang', 'Jojung', 'Tapdong', 'Anbaek', 'Dabun', 'Sugang', 'Keumgang', 'Jeokjung', 'Seodun', 'Joeun', 'Dajung', and 'Baekjung'. It seems that the 15 cultivars above showed the possibility of using AMT. On the other hand, 'Yeonbaek', 'Goso', 'Baekgang', and 'Johan' showed less than 20% and GUS spots were not observed in 'Gru', 'Gobun', 'Milseong', and 'Shinmichal-1'. This study explores transient GUS expression in Korean wheat cultivars seven days after AMT. The observed initial high efficiency of transient transformation suggests the potential for subsequent stable transformation efficiency. Korean wheat cultivars demonstrating elevated transient transformation efficiency could serve as promising candidates for the development of stable transgenic wheat.
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