• 동의생리병리학회지
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• 제24권5호
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• pp.866-871
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• 2010

The antidiabetic effects of Rosa rugosa Radix were investigated in streptozotocine-induced diabetic rats. Research methods and procedure: In the present study, effects of oral administration of Rosa rugosa Radix extract(100, 250, and 500 mg/kg body wt.) for 28 days on the level of serum glucose, total cholosterol, triglycerides, cereatinine, aspartate amino transferase(AST) and alanine amino transferase(ALT) in normal and streotozotocine-induced diabetic rats were evaluated. Oral administrations of the Rosa rugosa Radix extract significantly decreased serum glucose, total cholesterol, triglyceride, AST, and ALT levels, while increased serum insulin and HDL-C in diabetic rats(p<0.05). The hypoglycemic effect of the Rosa rugosa Radix extract was more effective than normal group. It is concluded that the Rosa rugosa Radix must be considered as excellent candidate for future studies on diabetes mellitus.

• The Korean Journal of Physiology and Pharmacology
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• 제20권4호
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• pp.333-340
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• 2016

Edaravone, a synthetic-free radical scavenger, has been reported to reduce ischemia-reperfusion-induced renal injury by improving tubular cell function, and lowering serum creatinine and renal vascular resistance. The present study investigated the effect of edaravone in diabetes mellitus-induced nephropathy in rats. A single administration of streptozotocin (STZ, 55 mg/kg, i .p.) was employed to induce diabetes mellitus in rats. The STZ-administered diabetic rats were allowed for 10 weeks to develop nephropathy. Mean body weight, lipid alteration, renal functional and histopathology were analysed. Diabetic rats developed nephropathy as evidenced by a significant increase in serum creatinine and urea, and marked renal histopathological abnormalities like glomerulosclerosis and tubular cell degeneration. The kidney weight to body weight ratio was increased. Moreover, diabetic rats showed lipid alteration as evidenced by a significant increase in serum triglycerides and decrease in serum high-density lipoproteins. Edaravone (10 mg/kg, i .p., last 4-weeks) treatment markedly prevented the development of nephropathy in diabetic rats by reducing serum creatinine and urea and preventing renal structural abnormalities. In addition, its treatment, without significantly altering the elevated glucose level in diabetic rats, prevented diabetes mellitus-induced lipid alteration by reducing serum triglycerides and increasing serum high-density lipoproteins. Interestingly, the renoprotective effect of edaravone was comparable to that of lisinopril (5 mg/kg, p.o, 4 weeks, standard drug). Edaravone prevented renal structural and functional abnormalities and lipid alteration associated with experimental diabetes mellitus. Edaravone has a potential to prevent nephropathy without showing an anti-diabetic action, implicating its direct renoprotection in diabetic rats.

• Journal of Nutrition and Health
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• 제30권4호
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• pp.415-424
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• 1997

Serum lipid levels and fatty acid composition of serum phospholipids(PL) were investigated in 24 healthy male subjects who consumed either corn oil(CO) rich In linoleic acid(LA), perilla oil(PO) rich in $\alpha$-linolenic acid($\alpha$-LNA), or canola oil acid(OA) as a major fat source fir 5 weeks. The PO and the CNO groups showed significant(P <0.05) increases in serum high density lipoprotein-cholesterol(HDL-C) levels and in ratios of HDL- C/total cholesterol(TC) compared with initial values measured at the beginning of the study. Significantly(p <0.05) increased concentrations of serum triglycerides(TG) ware observed after 5 weeks of the CO based diet compared with both its intial value and the concentration observed after 5 weeks with the PO-based diet. fatty acid composition of serum PL reflected changes in dietary fatty acid composition and metabolism. Compared with the initial levels, significandy increased contents of eicosapentaenoic acid(EPA) and docosahexaenoic acid(DHA) were observed in serum PL of the PO group and significantly increased contents of $\alpha$-LNA and EPA were observed in the CNO group. Arachidonic acid(AA) content of serum PL did not change in the CO group during the study period, although, the increase in LA was significant(P <0.05). Compared with the CO-based diet, both the PO and the CNO-based diets seem to have beneficial effects on atherosclerosis by influencing the serum lipid profile and fatty acid composition of serum PL.

• Journal of Nutrition and Health
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• 제30권9호
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• pp.1073-1083
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• 1997

This project was to compare the effects of consuming diets incorporating soybean oil , canola oil, ad palm oil on blood serum lipid patterns and fecal fat excretion in healthy human adults. The project was composed of three studies that were run concurrently . Each study was composed of a 4-day pre-trial period and two , 14-day experimental periods arranged according to a cross-over design. During the pre trial period, subjects were allowed to eat self-selected diets. During the experimental period , subjects were asked to eat constant, laboratory controlled diets in which sources of dietary fat varied . Thirty healthy , young adult subjects were fed a laboratory controlled diet incorporating soybean oil, canola oil, and palm oil. Test oils provided approximately 20% of the total dietary calories form all sources. Total dietary fat from all sources was approximately 30% of consumed calories. The highest serum total cholesterol , LDL cholesterol, VLDL cholesterol , and triglyceride values occurred when pam oil diets were eaten. However, feeding on palm oil diets resulted in the same HDL cholesterol content as with soybean oil or canola oil diets. Soybean oil diets produced numerically lower blood serum total cholesterol , LDL cholesterol, and triglycerides, and numerically higher HDL cholesterol and VLDL cholesterol than did those with canola oil. Fecal fat excretion was numerically lower with soybean oil diets than with canola diets. The highest fecal fat excretion occurred when palm oil diets were eaten.

• 한국자원식물학회지
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• 제11권2호
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• pp.173-178
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• 1998

The feeding effects of Herba hottuynia extracts on the lipids profiles and the content of TAB-reactive substances were evaluated in rats fed a high cholesterol diet. Hot water or ethanol extracts of the dried leave were evaporated and lyophilized . The experimental animals wer edevided to four groups and fed the following diets for 4 weeks : BAsal (cholesterol-free diet), CHOL(cholesterol-enriched diet),CW (cholesterol plus water extract) and CE(cholesterol plus ethanol extract). Dietary cholesterol increased significantly the activities of serum GOT and GPT, but the extracts feeding (0.5% of diet) did not influence the activities induced by dietary cholesterol. Although dietary cholesterol increased significantly the concentrationof serum andliver cholesterol, it tended to decrease the concentation of serum triglycerides. CHolesterol feeding had a lowering effect on the lipid peroxidation value of serum, but not inliver.Furthermore, the extracts feeding, especially water extract, decreased markedly the liver peroxidation value. The results suggest that Houttyunia cordata extracts have an in vivo antioxidant effect, judged from the TBA value in the liver rats fed a high cholesterol diet.

• Journal of Nutrition and Health
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• 제35권4호
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• pp.414-420
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• 2002

동충하초 (Cordyceps militaris)의 자실체와 균사체가 고지방 식이를 섭취한 성숙된 흰쥐의 영양 생리활성에 미치는 영향을 평가하고자, 생후 35주령의 흰쥐에 표준식이를 급여한 정상군, 표준식이에 15% 돈지를 첨가한 식이를 급여한 고지방군, 고지방 식이에 자실체 및 균사체를 각각 3%씩 첨가한 식이를 급여한 군 등 4군으로 나누어 5주간 사육하였다. 실험동물의 체중증가, 간의 무게 및 식이효율은 고지방군에 비하여 자실체군 및 균사체군이 유의하게 감소되어 정상군과 비슷한 수준으로 체중과 간의 무게를 정상으로 유지시켜 주는 효과가 나타났다. 식이섭취량은 고지방군과 동충하초군들이 비슷한 수준을 유지하였다. 신장, 췌장 및 심장의 무게는 정상군과 각 실험군이 비슷하였다. 간의 총 지질과 콜레스테롤 농도 및 간과 혈청의 중성지질 농도는 고지방군에 비하여 자실체군 및 균사체군이 유의하게 감소되었다. 혈청의 총 콜레스테롤 및 인지질 농도는 정상군에 비하여 각 실험군이 유의하게 증가되었다. 혈청의 HDL-콜레스테롤 농도는 정상군에 비하여 자실체군 및 균사체군이 유의하게 증가되었다. 고지방군에 비하여 자실체군의 총 콜레스테롤에 대한 HDL-콜레스테롤 비율은 유의하게 증가되었고, 동맥경화지수는 감소되었다. 혈청의 총 단백질 농도는 정상군에 비하여 고지방군 및 균사체군이 유의하게 증가되었다. 혈청의 알부민, 혈당 및 혈색소 농도는 정상군과 각 실험군이 비슷하여 정상 수준을 유지하였다. 혈청의 glutamic pyruvic transaminase, glutamic oxaloacetic transaminase 및 ${\gamma}$-glutamyltr-anspeptidase의 활성은 정상군과 각 실험군이 유사한 경향을 보였다. Lactic dehydrogenase 활성은 고지방군에 비하여 자실체군 및 균사체군은 유의하게 증가되어 정상군과 비슷하였다. Alkaline phosphatase 활성은 정상군에 비하여 각 실험군이 유의하게 감소되었다. 이상의 결과로 보아 고지방 식이에 3%동충하초의 자실체와 균사체 섭취에 의한 체중과 간의 무게가 정상수준을 유지하였고, 간의 총 지질과 콜레스테롤 농도 및 간과 혈청의 중성지질 농도를 낮추는 효과가 있는 것으로 나타났다.

• 한국수산과학회지
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• 제34권3호
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• pp.173-178
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• 2001

저분자 alginate인 HAG-10, HAG-50, HAG-100 및 alginate를 랫드에 35일간 섭취시켰을 때, 혈청중의 지질성분과 동맥경화지수의 측정으로 특성이 가장 좋은 저분자 alginate인 HAG-50의 안전성을 확보하기 위해 랫드에 경구급성독성실험을 실시하였다. 혈청중 total cholesterol, HDL-cholesterol, LDL-cholesterol, triglycerides 및 phospholipid 함량과 동맥경화지수는$5\%$ 와 $10\%$HAG-50에서 모두 유의적으로 뚜렷히 감소하여 지질대사 개선효과를 나타내었다. HAG-50의 경구급성독성실험을 검토한 결과, 투여할 수 있는 최고 용량인 랫드 체중 kg당 5.0g의 용량에서도 암$\cdot$수 모두 일반상태의 이상 반응과 폐사 및 병리$\cdot$조직학적 이상을 나타내는 예는 전혀 관찰되지 않아서 $LD_{50}$치의 산출이 불가능하였으므로 HAG-50은 아주 안전한 제재임이 검증되었다. 이상의 결과를 종합해 볼 때, 평균분자량 50,000 정도의 저분자 alginate인 HAG-50이 가장 우수한 지질대사 개선효과의 기능적 특성을 발휘하였으며, 경구급성독성실험 에서도 독성이 전혀 없었고, alginate가 가지고 있는 고유의 생리적 기능을 유지 향상시키면서 이용에 제한이 되고 있는 점성을 줄이고 용해도를 높혀서 alginate의 이용성을 높일 수 있는 새로운 형태의 식품소재라고 사료된다.

• 한국지역사회생활과학회지
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• 제27권2호
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• pp.233-243
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• 2016

This study examined the antioxidant activity and lipid composition of rats fed a high fat-high cholesterol diet containing Salvia plebeia water extract. Three levels (50 mg/kg, 200 mg/kg, 500 mg/kg) of Salvia plebeia water extract were administered to seven weeks old male Sprague Dawley rats for four weeks. The activity of glutathione peroxidase in serum was high in all groups that received the Salvia plebeia water extract (p<0.05). The glutathione reductase activity was high (p<0.05) in groups that received 50 mg/kg and 500 mg/kg of Salvia plebeia water extract. When rats received Salvia plebeia water extract, the content of MDA (malondialdehyde) in the serum and liver was low in all groups. In addition, the serum total lipid, triglyceride, total cholesterol and LDL-cholesterol contents decreased significantly (p<0.05). Total cholesterol and triglyceride levels in the liver were significantly lower in the group that received the Salvia plebeia water extract than in the control group (p<0.05). Salvia plebeia water extract improves lipid metabolism and it almost meets with the increase of antioxidant activity and peroxide formation reduction. Based on these results, supplemented of a high fat-hypercholesterol diet with Salvia plebeia can increase antioxidant activity and depress peroxide formation in serum, as well as liver cholesterol and triglycerides.

• 셀메드
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• 제4권3호
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• pp.18.1-18.5
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• 2014

Leea asiatica (L.) Ridsdale, a folk medicinal plant is used by the ethnic people of North East India for the treatment of hepatic disorder. In this study, we have investigated the hepatoprotective and antioxidant activity of L. asiatica leaves against acetaminophen induced hepatotoxicity. Methanol extract of L. asiatica (150 and 300 mg/kg/day, p.o.) were administered to rats for three consecutive days followed by single acetaminophen (3000 mg/kg, p.o.) administration on $3^{rd}$ day. After 48 h of acetaminophen administration animals were sacrificed and biochemical estimation of serum, in vivo antioxidant activity using liver tissue were carried out. High levels of serum glutamate oxaloacetate transaminase, serum glutamate pyruvate transaminase, serum alkaline phosphatase, total bilirubin, direct bilirubin, total cholesterol and triglycerides were observed in disease control group, which found near to normal in extract treated groups. Higher dose exhibited significant hepatoprotective activity against acetaminophen induced toxicity. Level of superoxide dismutase, catalase, glutathione peroxidase in liver tissue, and reduced glutathione in liver and blood were also significantly increased in extract (300 mg/kg) treated animals compare to disease control group. In this study we found that leaves of L. asiatica exhibited potent hepatoprotective activity against acetaminophen induced hepatic damage in experimental animals which justify the folklore claim, and the possible mechanism of this activity may be due to strong antioxidant activities of extract.

• 한국식품영양학회지
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• 제21권3호
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• pp.328-335
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• 2008

The principal objective of this study was to assess the effects of chitosan oligosaccharide supplementation on the improvement of blood glucose, lipid components and enzyme activities in the serum of streptozotocin(STZ, 55 mg/kg B.W., I.P. injection)-induced hyperglycemic rats fed on experimental diets for 5 weeks. The concentrations of blood glucose, total cholesterol, atherosclerotic index, LDL, LDL-cholesterol, free cholesterol, cholesteryl ester ratio, triglycerides(TG) and phospholipids(PL) in serum were remarkably higher in the hyperglycemic group(group BSW) and STZ(I.P.)+chitosan oligosaccharide supplementation group(group ECW) than those in the control group(group BW, basal diet+water). However the concentrations of blood glucose, total cholesterol, atherosclerotic index, LDL, LDL-cholesterol, tree cholesterol, cholesteryl ester ratio, TG and PL in serum were lower in the ECW group than in the BSW group, whereas the ratio of HDL-cholesterol concentration to total cholesterol and HDL-cholesterol concentration in the ECW group were higher than in the BSW group. The activities of alkaline phosphatase(ALP) and aminotransferase(AST, ALT) in serum were lower in the ECW group than in the hyperglycemic BSW group. The results shown above suggested that chitosan oligosaccharide supplementation effectively improves blood glucose, lipid composition and enzyme activities in the sera of STZ-induced hyperglycemic rats.