• 한국동물위생학회지
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• 제15권1호
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• pp.32-45
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• 1992

In order to establish and enzyme-linked immunosorbent assay to ILTV, field virus strain of ILTV was propagated in chorioallantoic membrane of the embryonated eggs. purified and used as antigen. The antisera selected from the field samples and immunized chickens based on serum neutralization test were used as the standard positive and negative sera in all tests. It was found that optimal antigen concentration was $2{\mu}g$ of protein per well and a 1 : 100 dilution of standard serum showed low background optical density with negative serum and high P/N values of positive sera. A 1 : 500 dilution of the rabbit anti-chicken IgG peroxidase conjugate produced a high P/N values and thirty minutes was chosen as suitable time to read the optical density of the enzyme substrate reaction and optical density was consistent during the 16 hours after stopper was treated. When coated antigen was kept on microplate for varying time up to 16 hours at $4^{\circ}C$ or $37^{\circ}C,$ no significant difference was observed between the treatment. The coated antigen could be kept without change of antigenicity for at least one month at $-70^{\circ}C,\; -20^{\circ}C,\; 4^{\circ}C$ and room temperature. When blocking buffer contanining bovine serum albumin was mixed directly with conjugate and serum at 10% level induced higher P/N values compared to blocking antigen coated microplate with the same blocking buffer. The coefficience of correlation between ELISA and SN test was 0.577. When antibody response of chickens, vaccinated with ILTV, was examined by ELISA and SN test, antibody rising and decay pattern between the two test was similar until 11 weeks of age. However 12 weeks onward antibody titer checked on by SN test was slightly lower than that tested by ELISA.

• 생명과학회지
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• 제27권3호
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• pp.283-288
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• 2017

양돈산업에 있어 돼지 써코바이러스 2형(PCV2)의 복합감염으로 인한 이유자돈의 질병 피해가 막대하다. PCV2 감염에 대한 숙주의 민감도는 상이한 것으로 알려져 있으며, 따라서 숙주의 민감도를 구분하는 것은, 이를 이용한 숙주의 저항성 향상 연구에 필수이다. 본 연구의 목적은 이유자돈군의 혈액 내에서 PCV2 바이러스에 대한 숙주의 민감도를 구분 짓고 구명하는데 있었다. 본 연구에서는 자연적으로 바이러스에 감염된 10주령의 이유자돈군으로부터 혈청을 채취하여 PCV2 바이러스량과 항체가를 측정하고 혈구분석을 실시했다. 또한, 측정된 PCV2 바이러스량과 항체가를 기준으로 자돈군 내에서 저항성군과 민감성군을 선정하였고, 통계분석결과 저항성군에 비해 민감성군에서 백혈구 수가 현저히 줄어든 것을 확인하였다. 본 연구를 통해서 PCV2 감염에 대한 돼지의 민감도를 구분짓기 위한 PCV2 바이러스량과 항체가를 이용한 복합기준을 제시할 수 있었으며, 이유자돈군의 PCV2 관련 질병저항성 및 백혈구감소증을 확인할 수 있는 방법을 마련하였다.

• 한국동물위생학회지
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• 제26권4호
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• pp.323-328
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• 2003

A total of 419 slaughtered cattle were used to investigate the serum Ig G titers to the Mannheimia haemolytica Al whole cell and leukotoxin recognized with important virulence factor in bacterial pathogenesis. Data obtained in this study were represented with average absorbance${\pm}$standard deviation. Serum Ig G titers were detected with the ranges from 0.1 to 0.5 at 490nm. Whole cell titers were higher than leukotoxin antibody on the whole. Antibody titers of slaughtered cattle between races, ages have no significant difference but gradual decrease under aging in dairy cow for whole cell (decline mean titer from 0.29 to 0.27 according to age) was undertaken. Holstein bulls shipped from Seoul province had a significantly lower Ig G titers than those from another ones (p<0.05).

• 대한미생물학회지
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• 제15권1호
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• pp.39-46
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• 1980

This experiment has been made to evaluate the distribution of OEP-HA titer, protease-HA titer and elastase-HA titer of antibody which are the common antigen of Pseudomonas aeruginosa in the serum of healthy mother-newborn infant-pairs, term pregnancy women and under 7 month old infants. By analysing the normal limt of these antibodies, it is expected that the result can be clinically applicable to the diagnosis, management and prevention of Pseudomonas aeruginosa infection, which shows rapid increase nowadays, 1. The Anti OEP-HA titer showed under 1:8, a very low titer, in 73% of cord sera group, but over 1:32 in 68% of mother sera group. 2. In healthy mother-newborn infant-pairs group, the anti OEP-HA titer of mothers showed 1:56, 48, much higher than that of newborn infant, 1:16.42. from which it can be concluded that their titer has no relation between the two. 3. The anti OEP-HA titers of healthy mother and term pregnancy women showed 1:56.5 and 1:53, 4, respectively, which are very similar to each ether, but in infant group, the titer showed 1:33, 51 higher than that of cord sera, 1:16.42 4. The anti protease-HA titer showed under 1:8 in 64-77.5% of total cases, which is lower than that of anti OEP-HA titer. 5. The anti elastase-HA titer showed under 1:8 in 93.75% of cord sera group and in 70.27-76.37% of infant, term perenancy women and mother group, but in infant group, the anti elastase-HA titer showed 1:16.56 higher than that of cord sera group 1:8.5.

• The Korean Journal of Physiology and Pharmacology
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• 제24권2호
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• pp.185-191
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• 2020

Interstitial cells of Cajal (ICC) are known as the pacemaker cells of gastrointestinal tract, and it has been reported that acute gastroenteritis induces intestinal dysmotility through antibody to vinculin, a cytoskeletal protein in gut, resulting in small intestinal bacterial overgrowth, so that anti-vinculin antibody can be used as a biomarker for irritable bowel syndrome. This study aimed to determine correlation between serum anti-vinculin antibody and ICC density in human stomach. Gastric specimens from 45 patients with gastric cancer who received gastric surgery at Kangwon National University Hospital from 2013 to 2017 were used. ICC in inner circular muscle, and myenteric plexus were counted. Corresponding patient's blood samples were used to determine the amount of anti-vinculin antibody by enzyme-linked immunosorbent assay. Analysis was done to determine correlation between anti-vinculin antibody and ICC numbers. Patients with elevated anti-vinculin antibody titer (above median value) had significantly lower number of ICC in inner circular muscle (71.0 vs. 240.5, p = 0.047), and myenteric plexus (12.0 vs. 68.5, p < 0.01) compared to patients with lower anti-vinculin antibody titer. Level of serum anti-vinculin antibody correlated significantly with density of ICC in myenteric plexus (r = -0.379, p = 0.01; Spearman correlation). Increased level of circulating anti-vinculin antibody was significantly correlated with decreased density of ICC in myenteric plexus of human stomach.

• 한국축산식품학회지
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• 제34권3호
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• pp.339-345
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• 2014

This study was performed to develop a rapid immuno-assay kit, by using a specific antigen to detect Hanwoo brand meat. We selected a synthetic antigen specific to our target antibody, named BIO-TAG (Tyr-D-Ala-Phe), by utilizing a computer-based analysis and literature review. BIO-TAG tagged with adjuvant was subcutaneously injected in sheep and Hanwoo. The serum and meat juice of the immunized or non-immunized animal were then analyzed, to measure the titer of antibody by ELISA and Western blot. The amount of antibodies against the BIO-TAG increased (p<0.05) in serum by vaccination. Furthermore, meat juice from the immunized Hanwoo showed greater (p<0.05) antibody titer, compared with those from non-immunized groups. To optimze the dilution factor, we performed dot-ELISA, with various combination levels of BIO-TAG. Results from dot-ELISA showed that 2 mg/mL BIO-TAG was sufficient to distinguish the immunized meat from non-immunized groups. These results support our hypothesis that simple immunization of Hanwoo generates a sufficient amount of antibodies to be detectable in the meat juice by means of the immune-assay. Therefore, specific Hanwoo brand meat can be more precisely identified by our rapid diagnostic kit. This technology can deter possible fraud of counterfeit meat brands in the Korean domestic market with ease and rapidity; and offers a new tool that guarantees consumers high quality Hanwoo brand beef.

• 한국동물위생학회지
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• 제30권3호
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• pp.329-338
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• 2007

Samples collected from 15 broiler farms(47 flocks, 920 1-day-old chicks) during March to December, 2006, To survey serum antibody titers of NDV, IBDV and MG/MS, the antibodies of ND viruses were detected by HI test and ELISA, against antibodies of IBD viruses and MG/MS by ELISA. The antibody titers of NDV showed 6.4, HI and 6,968, ELISA, respectively. The rate to below protective antibody levels(${\ge}5$, HI and ${\ge}1,000$, ELISA) were 8%, HI, 5%, ELISA, specially, Baeksemi were 22%, HI, 14%, ELISA. The rate of positive by ELISA showed 99%(914/920). The ELISA titer of IBDV showed mean titer 3,890. The rate of positive were 93% (857/920), specially, Baeksemi were 84%. The ELISA titers of MG/MS showed mean titer 5,666. The rate of positive were 78% (715/920) and 100%, Abor-Acre, 97%, Baeksemi, respectively. The antibodies not detected from 18%, ELISA titers was varied from 500 to 20,000. At antimicrobial susceptibility of E coli, Staphylococcus spp and Salmonella spp isolated from 1-day-old chicks, E coli were susceptible to AmC, AM, NOR, SXT, ENR, CIP, Staphylococcus spp were susceptible to AmC, SXT, AM, ENR and Salmonella spp were susceptible to AM, AmC, SXT and P.

• 대한수의학회지
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• 제56권2호
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• pp.121-123
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• 2016

A 7-year-old castrated male domestic short-hair cat presented with anorexia, constipation, depression, and voice alteration. Physical and neurological examinations revealed hyperthermia ($40.5^{\circ}C$), ventroflexion of the neck, reduced responses to external stimuli, generalized muscle weakness, and exercise intolerance. Thoracic radiographs revealed the presence of a cranial mediastinal mass. The history, clinical signs, and other examination results were compatible with acquired myasthenia gravis (MG). Acetylcholine receptor (AChR) antibody titers were determined to confirm MG and the serum AChR antibody concentration was 1.24 nmol/L (reference interval, < 0.3 nmol/L). This is the first diagnosis of acquired MG in a cat in Korea.

• Journal of Animal Science and Technology
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• 제44권5호
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• pp.633-642
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• 2002

1. ETEC F41 균주로부터 분리한 섬모항원의 분자량은 29.5 kDa으로 나타났으며, western blot을 통하여 섬모항원임을 확인하였다. 2. 분리한 섬모항원의 농도를 50 ${\mu}g$/$m\ell$, 200 ${\mu}g$/$m\ell$, 600 ${\mu}g$/$m\ell$로 조정 후 산란계에 접종하였다. 이 후 ELISA법을 이용하여 난황의 항체역가를 측정한 결과 최고치가 320,000(antigen 50${\mu}g$/$m\ell$), 450,000(antigen 200${\mu}g$/$m\ell$), 320,000(anti- gen 600${\mu}g$/$m\ell$)으로 나타났다. 3. F41난황항체와 K88, K99, 987P 섬모항원과의 교차반응을 ELISA법을 이용하여 조사해본 결과 난황항체를 30,000배 희석 시 교차반응이 없었다. 4. 실험실조건하에서 난황항체의 항원결합능력을 조사한 결과, 동결건조한 WSF을 2${\sim}$4 mg/$m\ell$ 첨가 시 균체의 농도가 $10^9$ CFU/$m\ell$에서 $10^5$ CFU/$m\ell$로 급격하게 감소하였다.

• 대한수의학회지
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• 제25권1호
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• pp.69-75
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• 1985

Hog cholera serum neutralizing antibody of piglets and sows were titrated by means of END SN method. The piglets of a variety of ages, precolostrally immunized with LOM living HC vaccine were subjected to the test. The sows were vaccinated with lapinized living HC vaccine after 25 days from the parturition. Throughout the studies the following results were obtained and summarised. 1. Hog cholera antibody titers of inbred sows immunized with lapinized living HC vaccine after 25 days from parturition were high except Hampshire group(Table 2). 2. Sows, different stage of the pregnancy or the day of parturition, and of 3 way crossed, that were immunized with lapinized living HC vaccine have shown no significant difference on HC antibody titer(Table 2, 3). 3. HC antibody titers of piglets, immunized with a single dose of LOM HC vaccine before feeding colostrum, were high in case of the younger group(1 week) compare to the older(7 week) (Table 4). 4. The piglets that were booster immunized with LOM HC vaccine at the age of 7 weeks have shown an inconsistent antibody titers(Table 5).