• 한국식품저장유통학회지
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• 제31권3호
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• pp.486-498
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• 2024

본 연구는 만성 알코올 유도 쥐에 대한 1.5% 헛개나무 열매 농축액이 첨가된 콩나물 혼합물의 숙취해소 및 간 기능 개선 효과를 확인하고자 하였다. 헛개나무 열매 농축액을 함유한 콩나물 혼합물군(SHM) 내 ampelopsin 및 L-asparagine의 함량은 10.52, 35.19 ppm으로 확인되었으며, 만성 알코올 급여에 의해 체중 변화량이 감소하고, 간 중량은 증가한 반면 SHM 처리시 유의적으로 체중 변화량이 증가하였으며, 간 중량이 감소하였다. 혈중 alcohol 및 acetaldehyde의 농도는 SHM군에서 2.89, 0.53 g/L로 가장 낮은 함량을 보였으며, 간 조직 ADH 및 ALDH의 활성은 SHM군에서 33.11, 102.06 U/L로 가장 높은 활성을 나타냈다. 만성 알코올 유도는 ALT, AST 및 GGT와 같은 간 기능 지표 효소들의 활성을 증가시켰지만, SHM 처리시 각각 41.00, 130.83 및 0.47 U/L로 활성이 유의적으로 감소하였다. 간 조직 및 혈중 triglyceride 함량은 SHM군에서 1.45와 33.00 mmol/L로 가장 낮은 값으로 나타났고, 만성 알코올 투여로 증가된 혈중 total cholesterol 및 LDL cholesterol 함량은 SHM에 의해 감소하였으며, 만성 알코올 투여로 인해 감소된 혈중 HDL cholesterol 함량은 SHM 처리시 증가하였다. 간 조직을 형태학적 및 병리학적으로 관찰한 결과, 만성 알코올 유도군의 간 조직은 형태학적 관찰에서 황갈색을 띠었고, 병리학적 관찰에서는 지질방울의 크기 및 수가 증가하였다. 그러나 SHM 처리시 형태학적으로 관찰한 간 조직의 색은 적갈색으로 나타났고, 병리학적으로 관찰한 간 조직의 지질방울 크기 및 수는 감소하였다. SHM군의 간 조직 및 혈중 지질 과산화 함량은 677.68, 76.26 nmol/g으로 가장 낮은 값으로 확인되었으며, 간 조직 및 혈중 GSH 함량은 SHM군에서 가장 높은 값인 19.93 및 20.08 μmol/kg으로 나타났다. 따라서 콩나물 또는 헛개나무 열매의 단일 섭취보다 혼합하였을 때 ampelopsin 및 asparagine과 같은 유효성분들의 시너지에 의해 SHM군에서 증진된 숙취해소 및 간 기능 개선 효과를 확인할 수 있었으며, 이는 기능성 식품 소재화에 대한 가능성을 제시하는 기초 자료로 활용될 수 있음을 시사한다.

• Asian Pacific Journal of Cancer Prevention
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• 제14권3호
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• pp.1833-1840
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• 2013

Metastasis is one of the hallmarks of malignant neoplasms and is the leading cause of death in many cancer patients. A major challenge in cancer treatment is to find better ways to specifically target tumor metastasis. In this study, the anti-metastatic potential of the methanolic extract of Rhizophora apiculata (R.apiculata) was evaluated using the B16F-10 melanoma induced lung metastasis model in C57BL/6 mice. Metastasis was induced in C57BL/6 mice by injecting highly metastatic B16F-10 melanoma cells through the lateral tail vein. Simultaneous treatment with R.apiculata extract (10 mg/kg b.wt (intraperitoneal) significantly (p<0.01) inhibited pulmonary tumor nodule formation (41.1 %) and also increased the life span (survival rate) 107.3 % of metastatic tumor bearing animals. The administration of R.apiculata extract significantly (p<0.01) reduced biochemical parameters such as lung collagen hydroxyproline, hexosamine, uronic acid content, serum nitric oxide (NO), ${\gamma}$-glutamyl transpeptidase (GGT) and sialic acid levels when compared to metastasis controls. These results correlated with lung histopathology analysis of R.apiculata extract treated mice showing reduction in lung metastasis and tumor masses. Taken together, our findings support that R.apiculata extract could be used as a potential anti-metastasis agent against lung cancer.

• Asian Pacific Journal of Cancer Prevention
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• 제13권8호
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• pp.3989-3995
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• 2012

The aim of the present investigation was to evaluate the effect of A.nilotica extract against Dalton's ascitic lymphoma (DAL) induced solid and ascitic tumors in BALB/c mice. Experimental animals received A.nilotica extract (10 mg/kg.bw) intraperitoneally for 10 and 14 consecutive days before induction of solid and ascitic tumors, respectively. Treatment with A.nilotica extract significantly decreased the development of tumor and percentage increase in body weight when compared to DAL induced solid tumor control group, also increasing the life span, restoring the total white blood cell count and hemoglobin content and significantly decreasing the levels of serum aspartate transaminase (SGPT), alanine transaminase (SGOT), alkaline phosphatase (ALP), gamma glutamyl transferase (GGT) and nitric oxide (NO) when compared to DAL induced ascitic tumor controls. The treatment also reduced significantly the cellular glutathione (GSH) and nitric oxide levels in treated animals. Histopathological studies also confirmed protective influence. The outcome of the present work indicates that A.nilotica extract could be used as natural anticancer agent for human health.

• 대한한방내과학회지
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• 제28권1호
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• pp.115-123
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• 2007

Objectives : To investigate the correlations among alcohol consumption, alcoholic liver disorders, physical symptoms, and behaviors in heavy drinkers. Methods : 53 males who self-realized their severe alcohol consumption were enrolled in this study. 10 answers for a questionnaire, serum parameter, sonographic finding and body mass index were attained. The correlations between them were analyzed using Pearson's correlation and Student's t-test. Results : The average consumption of alcohol in these subjects was 2.5-fold over social drinkers. The incidence of alcoholic hepatitis was around 30%, while fatty liver 73%, and abnormal GGT 77%, respectively. No specific correlation between average volume of daily alcoholic consumption and alcohol-related hepatic parameters was shown in this study, but correlative tendency between fatty liver and body mass index was exhibited. Conclusions : This study may indicate that alcoholic liver injuries are caused by not just volume of alcohol consumed but more mixed factors including inherited genetic components, body fat mass, foods and other physical or emotional stress.

• 동의생리병리학회지
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• 제24권3호
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• pp.439-445
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• 2010

Pretreatment with Taraxacum Mongolicum H(TMH) prior to the administration of on $CCl_4$ significantly prevented the increased serum enzymatic activity of aminotransferase(ALT, AST), gamma-glutamyl transpeptidase(GGT) and bilirubin concentration in dose-dependent manner. In addition, pretreatment with TMH also significantly restored the elevation of hepatic malondialdehyde formation and the depletion of reduced glutathione content in the liver $CCl_4$-intoxicated rats. The restoration of microsomal aniline hydroxylase and aminopyrine N-demethylase activities indicated the improvement in functional status of endoplasmic reticulum. $CCl_4$-induced hepatotoxicity was also essentially prevented, as indicated by a liver histopathologic study. TMH showed antioxidant effects in $FeCl_2$-ascorbate-induced lipid peroxidation in rat liver homogenate and in superoxide radical scavenging activity. Our results suggest that the protective effect of TMH against $CCl_4$-induced hepatotoxicity possibly involve mechanisms related to its ability to block p450-mediated $CCl_4$ bioactivation and free radical scavenging effects.

• 대한수의학회지
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• 제53권3호
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• pp.181-184
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• 2013

Dystrophic calcinosis cutis associated with spontaneous hyperadrenocorticism was diagnosed in a 8-year-old female Chihuahua dog with erythematous, erosive, numerous papules, plaques, and crusts on the bilateral trunk, and inguinal region. Serum biochemical abnormalities included increases in alkaline phosphatase (ALP), alanine aminotransferase (ALT), gamma-glutamyl transpeptidase (GGT), and cholesterol. Radiographs showed mild hepatomegaly and subcutaneous lobulated calcific deposits. Histopathologic examination demonstrated diffuse deposition of basophilic calcified material in the dermis. Von Kossa's stain confirmed calcium deposition. Therapy with diltiazem was useful in resolving calcinosis.

• 한국어병학회지
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• 제17권2호
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• pp.113-121
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• 2004

This study was performed to investigate the pathphysiological side effects of chloramphenicol (CAP) on olive flounder, Paralichthys olivaceus. 150 mg/kg/bw/day of CAP was orally administered forcedly to flounder for 4 days and then the flounder was monitored for 11 days. The level of hematocrit was significantly decreased from 2 day to 3 day of CAP treatment in CAP-treated group. The levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) and gamma glutamyl transferase (GGT) were slightly increased during the treatment of CAP and gradually decreased after the treatment of CAP. The levels of blood urea nitrogen (BUN) and creatinine kinase (CK), although not significant, were slightly increased during the treatment of CAP. The level of lactate dehydrogenase (LDH) was not changed during the treatment of CAP but slightly increased after the treatment of CAP. However, no histopathological changes were observed in various organ after the treatment. From these results, it is suggest that CAP can evoke several side effects on haematopoietic organs in olive flounder.

• 한국임상수의학회지
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• 제22권2호
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• pp.170-173
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• 2005

A 4-year-old female Shunauzer dog was referred to the Veterinary Teaching Hospital of Chungbuk National University due to anorexia and depression. The dog had a history of regular walking on grass fields, weight loss, and hyperthermia $(40.6^{\circ}C)$. In the physical examination, lymph node enlargement was confirmed. Complete blood count result revealed leukocytosis and thrombocytopenia but there was no decreasing of red blood cells. On blood chemistry, serum ALP, GGT, CPK, and LDH were elevated. Abdominal radiograph showed splenomegaly. Anaplasma platys infection was suspected with inclusion body-like substances in platelets on blood smear. Anaplasma platys was confirmed by PCR. On the basis of laboratory examination, final diagnosis was anaplasmosis. Treatment was followed for 3 months with tetracycline and doxycycline. The patient was monitored every week during the treatment. The patient has recovered to normal condition without any clinical signs. We are going to emphasize the need of PCR technique in diagnosis and to report the possibility of long term treatment more than two months in rickettial disease.

• 한국식품영양학회지
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• 제29권3호
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• pp.307-312
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• 2016

This study was performed to investigate the effects of Acer tegmentosum Maxim. extract (ATE) on non-alcoholic fatty liver in Sprague Dawley (SD) rats. During oral administration of ATE, non-alcoholic fatty liver was induced by treatment with DL-ethionine. The lipid, total cholesterol (T-CHO) and malondialdehyde (MDA) in the liver tissue of ATE-fed rats showed lower levels, as compared to ATE-unfed rats. In ATE-fed rats, the serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and ${\gamma}$-glutamyl transferase (GGT) were lower than the case of ATE-unfed rats. Oil red staining of the liver showed that the lipid deposits were decreased by feeding ATE. These results strongly indicated that ATE has positive effects of protection against non-alcoholic fatty liver formation.

• 대한한방내과학회지
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• 제40권2호
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• pp.201-207
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• 2019

Objectives: The purpose of this case study is to report the effect of Korean Medicine treatment on a patient diagnosed with drug-induced liver injury by oral administration of acetaminophen. Methods: A 21-year-old male patient with drug-induced liver injury visited the clinic five times from January 28th, 2019 to March 16th, 2019. The patient took Saenggan-tang extract during the treatment period. Results: The levels of serum alanine aminotransferase (ALT) and gamma-glutamyl transferase (GGT) were significantly decreased. The chief complaints of the patient, such as yellow-red urine and fatigue, were also relieved. Conclusion: Traditional Korean medicine has a beneficial effect on drug-induced liver injury.