• Title/Summary/Keyword: Sericulture

Search Result 678, Processing Time 0.019 seconds

Cryopreservation of winter-dormant mulberry buds using two-step freezing

  • Hyeok Gyu Kwon;Kee Young Kim;Seul Ki Park;Chan Young Jeong;Sang-Kug Kang;Ik-Seob Cha;Seong-Wan Kim;Seong-Ryul Kim;Hyo-Eun Lee;Haeng-Hoon Kim;Jong Woo Park
    • International Journal of Industrial Entomology and Biomaterials
    • /
    • v.47 no.2
    • /
    • pp.126-133
    • /
    • 2023

  • Genetic resources of mulberry trees are commonly preserved as trophosomes, which are vulnerable to environmental factors, such as natural disasters, diseases, and pests. This study establishes a basic protocol for ultra-low temperature cryopreservation of mulberry trees using a two-step freezing process. The procedure was established using the "Daeshim" variety and then tested on genetic resources from 24 other mulberry varieties. Samples were first dried to a moisture content of 33-43% in a low-temperature forced-air chamber at -5 ℃, then slowly frozen from -5 ℃ to -20 ℃, and preserved in liquid nitrogen (-196 ℃). To determine the regeneration rate, isolated dormant buds were inoculated into MS basal medium, and grown shoots were grafted onto 1-year-old rootstock via chip budding and then cultured. After freezing in liquid nitrogen, the "Daeshim" variety exhibited a survival and regeneration rate of more than 70% and 50%, respectively. Applying the two-step freezing process to genetic resources from 24 mulberry species yielded average survival and regeneration rates of 85.3% and 75.5%, respectively. Morus alba showed survival and regeneration rates of 100%, confirming the efficacy of the two-step freezing method. These results indicate the high feasibility of ultra-low-temperature cryopreservation through two-step freezing of dormant buds from mulberry genetic resources. Additional research is required into the variations in regeneration rates with freezing period in liquid nitrogen.

  • https://doi.org/10.7852/ijie.2023.47.2.126 인용 PDF

Identification of Superior Polyvoltine Hybrids (polyvoltine${\times}$bivoltine) of Silkworm, Bombyx mori L.

  • Rao, C.G.P.;Chandrashekharaiah;Basha, K.Ibrahim;Seshagiri, S.V.;Ramesh, C.;Nagaraju, H.
    • International Journal of Industrial Entomology and Biomaterials
    • /
    • v.8 no.1
    • /
    • pp.43-49
    • /
    • 2004

  • Ten promising polyvoltine mulberry silkworm strains(SDMGl, SDMG2, SDMG3, SDMG4, SDMWl, SDMW2, RMWl, RMW2, RMW3 and RMW4) that are superior in quantitative and qualitative traits have been synthesized in the polyvoltine breeding laboratory of Andhra Pradesh State Sericulture Research ＆ Development Institute, Hindupur through systematic hybridization and appropriate selection methods. After the genotypes were found homozygous for the desired traits, they have been crossed with 3 bivoltine testers $(APS8, APS4 and {NB_2}{D_4})$ and thirty new hybrid combinations were developed for the assessment of their hybrid performance. Phenotypic expressions of economically important quantitative and qualitative traits of fist filial generation were measured and subjected for statistical analysis. Evaluation Index and Subordinate Function methods were employed for the assessment of hybrid performance since they are widely used in silkworm hybrid evaluation. Total of seven poly${\times}$bivoltine combinations, which ranked high in both the methods, were selected as potential combinations for further field test. These combinations also ranked significantly higher than the control hybrid (APMl${\times}$APS8).

  • PDF KSCI

Antibacterial and antifungal effects of Korean propolis against ginseng disease

  • Kim, Sung-Kuk;Woo, Soon Ok;Han, Sang Mi;Bang, Kyeong Won;Kim, Se Gun;Choi, Hong Min;Moon, Hyo Jung;Lee, Sung-Woo
    • International Journal of Industrial Entomology and Biomaterials
    • /
    • v.39 no.2
    • /
    • pp.82-85
    • /
    • 2019

  • We investigated the anti-microbial activity of propolis against the pathogenic bacteria and fungi on ginseng. We selected six microbials that caused postharvest root rots in ginseng. Propolis extracts were prepared by using the ethanol extraction method. We seeded the bacteria and fungi related to ginseng disease on a specific culture medium, and treated it with propolis extracts by using the paper disc method. Propolis extracts indicate the anti-microbial activity against Paenibacillus polymyxa, Fusarium solani, Rhizoctonia solani AG-1 and Pythium ultimum. However, the anti-fungal activity of propolis is weak on Pseudomonas fluorescens subsp. Cellulosa and Colletotrichum gloeosporioides. As a result, the antimicrobial effects of propolis against microbial that prevent ginseng growth were confirmed. The antimicrobial effects are shown according to the concentration of propolis against root rot. The fungi also showed antibacterial effects in a dose-dependent manner.

  • https://doi.org/10.7852/ijie.2019.39.2.82 인용 PDF KSCI