• Title/Summary/Keyword: Serial test

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Study on the Genetic Variation of the Mitochondrial DNA in the Beet Armyworm, Spodoptera exigua (H bner), Using PCR-RFLP (PCR-RFLP를 이용한 파방나방 (Spodoptera exigua(H bner)) 미토콘트리아 DNA의 유전변이 연구)

  • 김용균;이명렬;정충렬
    • Korean journal of applied entomology
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    • v.37 no.1
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    • pp.23-30
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    • 1998
  • Restriction fragment length polymorphism (RFLP) of a DNA has been a useful tool for analyzing genetic variation. This research was performed to establish an RFLP analytic method on the mitochondrial DNA (mtDNA) of the beet armyworm, Spodoptera exigua (Hiibner). To do this, total size of the mtDNA was measured and polymerase chain reaction (PCR) primers were selected. Its mitochondrial genome size was ca. 16kb. From a serial PCR test of 29 primers refered to the compilation of Simon et al. (1994), 22 primers were selected to amplify its mtDNA fragments. These primers resulted in short (300-700 bp) or long (1000-2000 bp) DNA products which represented a total or partial sequence of each of CO-I, CO-11, Cyt-B, ND-1, 12s rRNA, 16s rRNA, and some tRNAs. PCR-RFLP was performed in some variable mtDNA regions with 8 kinds of 4bp recognizing restriction enzymes. Different populations from Andong, Kyungsan, and Sunchun did not show any restriction site polymorphisms but had some length variation in certain regions of mtDNA.

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A Detection Method of Position of ON/OFF-Switch (ON/OFF-스위치의 위치 인식 방법)

  • Cho, Byung-Mo;Lee, Kwon-Yeon;Son, Myung-Sik
    • Journal of IKEEE
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    • v.11 no.1 s.20
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    • pp.30-37
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    • 2007
  • This paper proposes a detection method of position of OFF-switch. Each switch has the parallel path with a serial combination of passive element, its parallel path has each different frequency characteristics. Frequency characteristic of ON-switch reveals a flat spectrum irrelevant to frequency characteristic of passive element connected in parallel to its each terminal and frequency characteristic of OFF-switch reveals the same characteristic as one of passive element connected in parallel. Detection of position of OFF-switch is done by measuring the similarity of each spectrum corresponding to frequency characteristic of passive element connected in parallel to OFF-switch. The measure of their similarity is to calculate Euclidean distance between their test spectrum and reference spectrum. The spectrum with the smallest distance among reference spectrum is recognized as the spectrum of OFF-switch. The real time digital signal processing system is implemented to detect the position of OFF-switch by using spectrum matching.

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Investigation of the cytotoxicity of thermoplastic denture base resins

  • Lee, Jung-Hwan;Jun, Soo-Kyung;Kim, Si-Chul;Okubo, Chikahiro;Lee, Hae-Hyoung
    • The Journal of Advanced Prosthodontics
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    • v.9 no.6
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    • pp.453-462
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    • 2017
  • PURPOSE. The purpose of this study was to investigate the in vitro cytotoxicity of thermoplastic denture base resins and to identify the possible adverse effects of these resins on oral keratinocytes in response to hot water/ food intake. MATERIALS AND METHODS. Six dental thermoplastic resin materials were evaluated: three polyamide materials (Smile tone, ST; Valplast, VP; and Luciton FRS, LF), two acrylic materials (Acrytone, AT; and Acryshot, AS), and one polypropylene resin material (Unigum, UG). One heat-polymerized acrylic resin (Vertex RS, RS) was chosen for comparison. After obtaining extracts from specimens of the denture resin materials (${\phi}=10$ mm and d=2 mm) under different extraction conditions ($37^{\circ}C$ for 24 hours, $70^{\circ}C$ for 24 hours, and $121^{\circ}C$ for 1 hour), the extracts (50%) or serial dilutions (25%, 12.5%, and 6.25%) in distilled water were co-cultured for 24 hours with immortalized human oral keratinocytes (IHOKs) or mouse fibroblasts (L929s) for the cytotoxicity assay described in ISO 10993. RESULTS. Greater than 70% viability was detected under all test conditions. Significantly lower IHOK and L929 viability was detected in the 50% extract from the VP ($70^{\circ}C$) and AT ($121^{\circ}C$) samples (P<.05), but only L929 showed reduced viability in the 50% and 25% extract from LF ($37^{\circ}C$) (P<.05). CONCLUSION. Extracts obtained from six materials under different extraction conditions ($37^{\circ}C$, $70^{\circ}C$, and $121^{\circ}C$) did not exhibit severe cytotoxicity (less than 70% viability), although their potential risk to oral mucosa at high temperatures should not be ignored.

Effects of Action Oriented Breast Self-examination(BSE) Education on Knowledge, Self-efficacy, and Performance Competence in Nursing Students (행위중심적 유방자가검진 교육이 간호학생의 지식, 자기효능감 및 수행능력에 미치는 효과)

  • Park, So-Mi;Hur, Hea-Kung;Park, Mi-Jeong;Song, Hee-Young
    • Women's Health Nursing
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    • v.12 no.1
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    • pp.47-52
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    • 2006
  • Purpose: This study was undertaken to test effects of action oriented BSE education on knowledge, selfefficacy, and performance competence in nursing students. Methods: A non-equivalent pre-posttest of time serial quasi-experimental design was used to prevent diffusion of the experiment. Out of 53 nursing students selected by convenient sampling in the third grade at a nursing college, 27 students were randomly assigned to the control group and were first given a traditional lecture on BSE. For the experimental group of 26 students action oriented BSE education consisted of lectures, demonstrations with breast models, and BSE skill practice by micro teaching. Results: There was no significant difference in general and BSE-related characteristics between the two groups. Scores of the experimental group in knowledge, self-efficacy, and performance after the intervention were significantly higher than those of the control group. Conclusions: Results showed that action- oriented BSE education was effective in increasing knowledge, self-efficacy and performance competence in nursing students. Education for students should incorporate strategies reinforcing confidence by practicing actual BSE skills as well as acquiring knowledge of BSE, so that students can become competent and practical cancer prevention educators.

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A Study on the Variation of Electric Contact Resistance Due to Change in Contact Force in a Tin-plated Connector (주석 도금한 커넥터의 접촉 하중의 변화에 의한 전기 접촉저항 변화에 관한 연구)

  • Yu, Hwan-Shin;Oh, Man-Jin;Park, Hyung-Bae
    • Journal of Advanced Navigation Technology
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    • v.18 no.4
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    • pp.381-386
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    • 2014
  • In order to investigate the effect of contact load, which is one of the fretting corrosion factors affecting the electric connector, a coupled fretting corrosion specimens were prepared using a tin-plated brass coupon with a thickness of $3{\mu}m$. Electric resistance of the contact was measured during the fretting corrosion test period. There was increase in resistance with fretting cycles. The change in resistance can be classified by 3 stages. The first stage exhibited low and stable resistance. Second stage showed steady increment of the resistance and third stage showed very high and intermittent resistance. The relationship between the failure cycle (Nf) and contact force (P) can be drawn as; It is possible to draw the prediction equation for the failure cycle of the electric connector corresponding to the very high and intermittent resistance under various environment conditions through the fretting tests under various conditions such as load, displacement, temperature.

Milk Containing BF-7 Enhances the Learning and Memory, Attention, and Mathematical Ability of Normal Persons

  • Kim, Do-Hee;Lee, Hyun-Jung;Choi, Gooi-Hun;Kim, Ok-Hyeon;Lee, Kwang-Gill;Yeo, Joo-Hong;Lee, Jun-Young;Lee, Sang-Hyung;Youn, Young-Chul;Lee, Jang-Han;Paik, Hyun-Dong;Lee, Won-Bok;Kim, Sung-Su;Jung, Hee-Yeon
    • Food Science of Animal Resources
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    • v.29 no.2
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    • pp.278-282
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    • 2009
  • Previous studies indicate that BF-7 enhances learning and memory in normal and elderly individuals. Here, we evaluated whether milk containing BF-7 (BF-7 milk) could improve the brain function, with thirty normal university students $(21{\pm}1.2 years)$. Two versions of the Paced Auditory Serial Addition Test were used under double-blinded conditions to measure the efficacy of BF-7 milk on learning and memory, especially working memory and attention, and on mathematical ability. As a result, BF-7 milk improved the accuracy of the task more than 3-fold. Furthermore, BF-7 milk protected cultured neuronal cells from 3-hydroxykynurenine, a normal endogenous brain stress agent. These results indicate that BF-7 milk enhances memory, attention and mathematical ability in normal persons.

Long-Term Management with Chlorambucil and Prednisolone in a Case of Feline Alimentary Lymphoma

  • Kim, Myung-Jin;Kim, Nam-Kyun;Jang, Hyo-Mi;Song, Joong-Hyun;Hwang, Tae-Sung;Lee, Hee-Chun;Sur, Jung-Hyang;Jung, Dong-In
    • Journal of Veterinary Clinics
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    • v.34 no.3
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    • pp.193-196
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    • 2017
  • A 14-year-old, castrated male, domestic shorthair cat was referred for gastrointestinal (GI) signs, including nausea, regurgitation, anorexia, and weight loss. Abdominal ultrasonography revealed thickening of the wall of the gastric and proximal duodenum, moderately enlarged mesenteric lymph nodes, and coarse echotexture of the splenic parenchyma. The results of the feline leukemia virus test were positive. Based on gastrointestinal endoscopic characteristics and histopathological examinations, low-grade alimentary lymphoma was identified in multiple regions of the gastrointestinal tract. The patient was treated with oral prednisolone and chlorambucil chemotherapy, and the clinical signs resolved gradually. During serial follow-up, ultrasonographic findings demonstrated decreases in the duodenal wall thickness and size of the abdominal lymph nodes over a period of 550 days. Survival time was 886 days with prednisolone and chlorambucil chemotherapy. This report describes clinical features, imaging findings, endoscopic characteristics, histopathological features, and long-term management with chlorambucil chemotherapy in a case of feline low-grade alimentary lymphoma.

Cell adaptation of KPEDV-9 and serological survey on porcine epidemic diarrhea virus(PEDV) infection in Korea (돼지 유행성설사바이러스 국내 분리주(KPED-9) 의 세포증식성 및 혈청학적 역학조사)

  • Kweon, Chang-hee;Kwon, Byung-joon;Kang, Yung-bai;An, Soo-hwan
    • Korean Journal of Veterinary Research
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    • v.34 no.2
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    • pp.321-326
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    • 1994
  • Korean isolate, porcine epidemic diarrhea virus (KPEDV-9) was adapted through serial passages in vero cell. The viral yield reached up to $10^{5-6}$ $TCID_{50}/ml$ at the passage level of 90th. The cell adapted virus was characterized through genetic and morphological examinations. The RNA extracted from virus infected cell revealed the presence of RNA band with molecular size of >20Kb. The electron microscopic examination on purified virus showed the pleomorphic appearance of enveloped particles with 5-10nm surface projections, which fit with the shape of coronavirus. The etiological survey on swine diarrhea by immunofluorescence test(FA) indicated 17.5% positive rate on the PEDV infection. In addition, the incidence were detected both in piglets within two weeks old as well as fattening pigs. Serological survey by ELISA revealed the overall 45% positive result, thus, indicating the PEDV infection are widespread throughout this country.

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A Case of Catamenial Hemoptysis treated successfully with Gonadotropin-releasing Hormone (GnRH) Analogue (Gonadotropin-releasing Hormone (GnRH) Analogue로 치유된 Catamenial Hemoptysis 1예)

  • Kim, Dae-Han;Suh, Yo-Ahn;Kim, Sang-IL;Choi, Kui-Sung;Son, Hyun-Bae;Kwon, Yoong-Ju;Kim, Sung-Ho;Kim, Cheol-Hyeon;Lee, Jae-Cheol
    • Tuberculosis and Respiratory Diseases
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    • v.53 no.3
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    • pp.349-353
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    • 2002
  • Catamenial hemoptysis is syndrome characterized by bleeding from the bronchial trees and lungs that occurs synchronously with the female menstrual cycle. Etiologic mechanism of pulmonary endometriosis is still controversial, and the diagnosis is usually made on the basis of the clinical history and exclusion of other causes of recurrent hemoptysis. Serial computed tomograms of the chest during and in the interval between menstruations have been proved to be a useful confirmatory test. We experienced a 33-year-old female patient who had been previously diagnosed as pelvic endometriosis pathologically, experienced cyclic hemoptysis during menstruations. The diagnosis of pulmonary endometriosis was made based on her history and changes in the character of the lesions as documented on radiologic studies of the chest. She was treated successfully with GnRH analogue and there is no evidence of recurrence.

The Gross Thrust Estimation Technique of Air-Breathing Engine (공기 흡입 엔진의 총추력 추정 기법)

  • Kim, Jeongwoo;Jung, Chihoon;Ahn, Dongchan;Lee, Kyujoon
    • Journal of the Korean Society of Propulsion Engineers
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    • v.22 no.3
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    • pp.97-108
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    • 2018
  • It is definitely important to measure thrust during ground test when developing air-breathing engine, and in case of air-breathing engine, gross thrust should be calculated considering not only the measured thrust but also the force induced by the air flow of engine intake. Also, side thrust like yaw and pitch should be measured and analyzed using multi-component thrust measurement system. Engine performance was accurately evaluated by calculating the gross thrust of air breathing engine precisely which is analyzed from below serial procedure: labyrinth seal isolation, 1-axis gross thrust calculation, develop multi-component thrust measurement system, and side thrust analysis.