• Journal of Microbiology and Biotechnology
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• v.20 no.1
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• pp.179-186
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• 2010

In biological wastewater treatment, high lipid concentrations can inhibit the activity of microorganisms critical to the treatment process and cause undesirable biomass flotation. To reduce the inhibitory effects of high lipid concentrations, a two-phase anaerobic system, consisting of an anaerobic sequencing batch reactor (ASBR) and an upflow anaerobic sludge blanket (UASB) reactor in series, was applied to synthetic dairy wastewater treatment. During 153 days of operation, the two-phase system showed stable performance in lipid degradation. In the ASBR, a 13% lipid removal efficiency and 10% double-bond removal efficiency were maintained. In the UASB, the chemical oxygen demand (COD), lipid, and volatile fatty acid (VFA) removal efficiencies were greater than 80%, 70%, and 95%, respectively, up to an organic loading rate of 6.5 g COD/l/day. No serious operational problems, such as significant scum formation or sludge washout, were observed. Protein degradation was found to occur prior to degradation during acidogenesis.

• Journal of the Korean Institute of Intelligent Systems
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• v.11 no.9
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• pp.844-848
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• 2001

This paper introduces unmaned fully automation systems, which are applied for the CSTR(Continuously Stirred Tank Reactor) and SBR (Sequencing Batch Reactor) wastewater treatment system. The pilot plant is constructed in the country side which is little far from a main city. So networks and wireless modules are employed for the data transmission. The SBR plant has a local control and the remote monitoring system which is contained communication parts which consist of ADSL (Asymmetric Digital Subscriber Line) network and CDMA (Code Division Multiple Access) Wireless module. Remote control and monitoring systems are constructed at laboratory in a metropolis.

• Journal of Korean Society of Water and Wastewater
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• v.38 no.1
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• pp.29-38
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• 2024

Wastewater management is increasingly emphasizing economic and environmental sustainability. Traditional methods in sewage treatment plants have significant implications for the environment and the economy due to power and chemical consumption, and sludge generation. To address these challenges, a study was conducted to develop the Intermittent Cycle Extended Aeration System (ICEAS). This approach was implemented as the primary technique in a full-scale wastewater treatment facility, utilizing key operational factors within the standard Sequencing Batch Reactor (SBR) process. The optimal operational approach, identified in this study, was put into practice at the research facility from January 2020 to December 2022. By implementing management strategies within the biological reactor, it was shown that maintaining and reducing chemical quantities, sludge generation, power consumption, and related costs could yield economic benefits. Moreover, adapting operations to influent characteristics and seasonal conditions allowed for efficient blower operation, reducing unnecessary electricity consumption and ensuring proper dissolved oxygen levels. Despite annual increases in influent flow rate and concentration, this study demonstrated the ability to maintain and reduce sludge production, electricity consumption, and chemical usage. Additionally, systematic responses to emergencies and abnormal situations significantly contributed to economic, technical, and environmental benefits.

• Journal of Korean Society on Water Environment
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• v.36 no.3
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• pp.220-228
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• 2020

Anaerobic ammonium oxidation (AMX) is a cost-efficient biological nitrogen removal process. The coexistence of ammonia-oxidizing bacteria (AOB) in an AMX reactor is an interesting research topic as a nitrogen-related bacterial consortium. In this study, a sequencing batch reactor for AMX (AMX-SBR) was operated with a conventional activated sludge. The AOB in an AMX bioreactor were identified and quantified using terminal restriction fragment length polymorphism (T-RFLP) and real-time qPCR. A T-RFLP assay based on the ammonia monooxygenase subunit A (amoA) gene sequences showed the presence of Nitrosomonas europaea-like AOB in the AMX-SBR. A phylogenetic tree based on the sequenced amoA gene showed that AOB were affiliated with the Nitrosomonas europaea/mobilis cluster. Throughout the enrichment period, the AOB population was stable with predominant Nitrosomonas europaea-like AOB. Two OTUs of amoA_SBR_JJY_20 (FJ577843) and amoA_SBR_JJY_9 (FJ577849) are similar to the clones from AMX-related environments. Real-time qPCR was used to quantify AOB populations over time. Interestingly, the exponential growth of AOB populations was observed during the substrate inhibition of the AMX bacteria. The specific growth rate of AOB under anaerobic conditions was only 0.111 d^-1. The growth property of Nitrosomonas europaea-like AOB may provide fundamental information about the metabolic relationship between the AMX bacteria and AOB.

• Journal of Korean Society of Water and Wastewater
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• v.12 no.1
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• pp.96-101
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• 1998

Substrate inhibition of 2,4-dinitrophenol (DNP) degradation was investigated using activated sludge which had been adapted to mineralize DNP. DNP is a metabolic uncoupler, preventing cells from making energy for growth and it has been suggested that pH may be important in mitigating effects of uncouplers. After acclimation of the activated sludge, the effect of pH on toxicity of DNP at high concentration (75 mg/L) was investigated, over a pH range of 5 to 9. DNP inhibition was found to be strongly dependent on mixed liquor pH. The DNP degradation rate was highest in the pH range of 6.95 to 7.84; at pH 5.94 degradation of 75 mg/L DNP was significantly inhibited; at pH < 5.77, DNP degradation was completely inhibited after approximately 30% of the DNP was degraded. By comparison, no significant effect of pH variation in the same range was seen on glucose uptake by the activated sludge culture.

• 한국생물공학회:학술대회논문집
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• 2000.04a
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• pp.105-108
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• 2000

Enhanced biological phosphorus removal (EBPR) is not always successfully achieved by anaerobic/aerobic operation. It has been reported that the EBPR deterioration was caused by the outgrowth of glycogen-accumulating organisms (GAO) over polyphosphate-accumulating organisms (PAO). It was found that pHcould be a tool which might induce the success of EBPR in a sequencing batch reactor (SBR) supplied with acetate. When the pH of anaerobic phase was controlled at 7.0, the operation resulted in failure of EBPR. However, when the pH of anaerobic phase increased up to 8.4, complete EBPR was achieved. We explained the mechanism of pH effect on the competition between GAO and PAO with experimental results and previously proposed biochemical models.

• 한국생물공학회:학술대회논문집
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• 2001.11a
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• pp.286-289
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• 2001

Anaerobic ammonium oxidation with nitrite to $N_2$(anammox) is a recently discovered microbial reaction with interesting potential for nitrogen removal from wastewater. Here we investigated the microbial community structure in the sequencing batch reactor(SBR) with an anammox activity. The SBR was optimized for the enrichment of a very slowly growing microbial community and showed that possibility of anaerobic ammonium oxidation. Fluorescence in situ hybridization(FISH) analysis revealed that anaerobic ammonium oxidizers were Candidatus Brocadia anammoxidans and Candidatus Kuenenia stuttgartiensis. Furthermore, Nitrosomol1as spp. of the ${\beta}$ -subclass of Proteobacteria was also present within the anaerobic SBR microorganisms.

• Journal of Environmental Health Sciences
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• v.34 no.1
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• pp.76-85
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• 2008

Biological nutrient removal (BNR) sludge was added to a sequencing batch reactor (SBR) in the addition ratios of 0%, 20%, 40%, 50% while observing the variation of nutrient removal characteristics and microorganism groups. When the BNR sludge was added in a ratio over 40%, the characteristics of EBPR (enhanced biological phosphorus removal) was shown at the 27 days. However, a distinct BNR was not shown when the addition ratio of BNR sludge was lower than 40%. The organic removal efficiency were shown as 90% in all SBRs irrespective of the addition ratio of BNR sludge. At the 27 days, the phosphorus removal efficiencies were shown as 40%, 55%, 77% and 69%, respectively, according to the addition ratio of BNR sludge. Overall, efficient nitrification and phosphorus removal was shown when the added BNR sludge ratio was over 40%.

• Journal of Environmental Health Sciences
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• v.35 no.2
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• pp.116-123
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• 2009

Laboratory scale experiments were conducted to investigate the removal characteristics of nitrogen and phosphorus by the variation of aeration time in four sequencing batch reactors (SBRs). In R1 which has the shortest aeration time as 1 h, MLVSS concentration in reactor decreased by the wash-out of biomass because of the poor sedimentation. The TOC removal efficiencies were almost similar in 3 reactors except R1. At the low aeration time as 1 h, the nitrification was severely inhibited by the deficiency of oxygen. ${NH_4}^+$-N removal efficiency was decreased by the decrease of aeration time. At the aeration time over 2 h, the phosphorus removal efficiency was not affected by the variation of aeration time. The nitrification was inhibited but the phosphorus release and uptake was not inhibited by the decrease of low aeration time. Therefore, we can see that the phosphorus removal microorganisms are superior to nitrification microorganisms in oxygen utilization.

• Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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• v.3 no.3
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• pp.169-175
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• 1999

2,4-Dinitrophenol(DNP) is a metabolic uncoupler that prevents cells from creating energy for growth and it has been suggested that the availability of sodium ions may be important in mitigating the effects of uncouplers. Accordingly, the degradation of DNP was investigated using activated sludge which had been adapted to mineralize DNP. After the acclimation of the activated sludge, the effect of sodium ions on the toxicity of high concentrations(80 to 100mg/L) of DNP was investigated over a sodium ion concentration range of 9.3$\times$10-5 to 94mM. The concentration of sodium ions in the activated sludge mixed liquor seemed to have little effect on the DNP toxicity. However, a lack of sodium in the grwoth media resulted in a reduction of the DNP degradation rate by a bacterial isolate from the activated sludge culture identified as Nocardia asteroides.