• 식물조직배양학회지
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• 제24권1호
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• pp.1-35
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• 1997

Fertilized egg, by successive cell divisions, differentiates into different tissues and organs with various structures and functions. Different cells and tissues contain different proteins, products of selective gene expression. Not all the genes in any genomes are equally active, temporal and spatial gene expression being the general rule. Present paper attempts to review the tanscriptional mechanisms or the initiations of transcription from several angles. In some of the organisms the genes in the process of transcription or the genes in the inactive state can be seen under the light microscope. Some bands of Drosophila polytene chromosomes may exhibit a swollen or puff appearance under certain conditions. A puff, unfolded or decondensed form of chromomere, represents sets of intense transcriptional activity or RNA synthesis. The heterochromatic X chromosome whose genes remain inactive in the female mammals can be visualized as a dark staining structure called Barr body, Configuration of chromatin differs between transcribed and nontranscribed chromatin. Modification to the chromatin facilitates RNA synthesis. The movement of large polymerase molecule along the DNA would probably be facilitated if some modifications of the chromatin configuration is effected. Methylation of cytosines in CG sequences is associated with inactive genes. Methylation can play a role in determination of mammalian cells during embryogenesis. Demethylation is necessary for the gene to be expressed during development A histone modification that is also known to be correlated with transcriptional capacity of chromatin is acetylation of the lysine residues of the core histones. Chromatin containing a high level of histone acetylation is very sensitive to DNase 1. For the transcription to occur TBP must first bind to the TATA box. Another TF, TF IIB, then binds to the promoter-TBP complex, facilitating the access of RNA polymerase to the transcription initiation site. As recently as eight years ago researchers assumed that histones were irrelevant to the regulation of gene expression. Histones combine with the DNA to form nucleosome of the chromatin. Histones are vital participant in gene regulation. Histone and basal factors compete for access to TATA box. When DNA is exposed to basal factors before histones are introduced, the basal factors assemble on TATA boxes preventing the access of histones, allowing transcription to occur, for transcription to begin, activator protein at the upstream activation sequence or enhancer must interact with the tail of histone H4 at TATA box and cause the histone role particle to dissociate from the TATA box leading to partial breakup of the histone core particle and allowing the basal factors to bind to the TATA box. New concept of genomic flux in contrast to the old concept of static genome has been developed based on the powerful new molecular techniques. Genomic changes such as repetitive DNAs and transposable elements, it is assumed but not yet proved, may affect some of the developmental patterns that characterize particular cells, tissues, organs, and organisms. In the last decade or so remarkable achievement have been made in the researches of the structures and functions of TFs and the specific target sequences located in promoters or enhancers where these TFs bind. TFs have independent domains that bind DNA and that activate transcription. DNA binding domain of TFs serves to bring the protein into the right location. There are many types of DNA binding domains. Common types of motifs can be found that are responsible for binding to DNA. The motifs are usually quite short and comprise only a small part of the protein structure. Steroid receptors have domains for hormone binding, DNA binding, and activating transcription. The zinc finger motif comprises a DNA binding domain. Leucine zipper consist of a stretch of amino acids with a leucine residue in every seventh position Two proteins form a dimer because they interact by means of leucine zippers on similar α-helical domain. This positions their DNA binding basic domains for interaction with the two halves of a DNA sequence with dyad symmetry of TGACTCA, ACTGAGT.

• 마케팅과학연구
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• 제19권3호
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• pp.49-61
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• 2009

本文为执行官提供了他们在处理日常业务问题和市场机会时如何阐述自己思考过程的培训. 本研究建立在Schank提出的教学基础上, 包括: (1)经验学习和最好的指导提供给学习者从诸如全球背景, 团队项目和专家经历等的互动的故事提炼知识和技能的机会. (2) 告诉不会导致学习, 因为在学习需要的行动训练环境中, 应强调积极使用故事, 案例和项目. 每个培训案例包括执行官解释自己的决策系统分析(DSA, 还需要执行官做DSA简报. 在训练时要求执行官写DSA简报. 在执行官学员写书面报告的说明中包括(1) DSA路线图的本质的细节(2) 警告和机会的陈述, 读者的行政地图及图内的DSA解释. 该报告的最大长度为500字, 其规则就是使行政人员培训课程行之有效. 引言之后是第二部分文献综述, 简要地总结了有关人们在对问题和机会的背景下的想法及文献. 第三部分通过使用对不同的贴牌生产客户定价相同的化学产品的培训练习来解释DSA的起源和过程, 第四部分展示一个炼油设备公司订价决策的培训练习. 第五部分提供一个商业客户办公家具采购的市场策略案例. 第六部分是结论和建议. 这些建议是关于使用培训课程和发展其他培训课程来磨练执行官制定决策的能力. 文章引导读者利用工具箱研究综合的报告, (DSA)路线图根据生态合理性理论将战略与环境相匹配. 这三个案例的研究让学习者在意愿层面征求建议来作出决策. Todd and Gigerenzer 提出人们使用简单启发式,因为他们在自然的决策环境中通过探索信息的结构使适应性行为有可能产生. "简单是一种美德, 而不是诅咒", 有限理性理论强调了西蒙的命题中心, "人类理性的行为仿佛一把剪刀, 其刀片则是任务环境的结构和执行者的计算能力". Gigerenzer的观点和西蒙的环境的危害相关, 也和本文中三个环境结构的案例相关. "环境这个词, 在这里, 并不是指总的物理和生理的环境, 而只是指被给予需要和目标的重要有机体 本文关注了结合任务环境的结构和使用适应的工具箱启发的报告. (DSA)路线图根据生态理性理论将战略与环境相匹配. 渴望适应理论是这一方针的核心. 渴望适应理论将决策制定作为一个没有把目标整合的多目标问题模拟成一个把所有决策选项进行完全的优先顺序化. 这三个案例研究让学习者在意愿层面征求建议来作出决策. 渴望适应用一系列的调整步骤的形式. 一个调整步骤通过仅一个目标变量的变化就可以改变在渴望网格上邻近点当前的渴望水平. 上调步骤是目标变量的提高, 下调步骤是目标变量的下降. 创造和使用渴望适应水平是对有限理性理论的整合. 文章通过提供学习者经验和实践环节增加了意愿采纳和有限合理性的理解和特点. 利用DSA图排列CTSs和撰写TOP可以清晰和深化Selten的观点 "清晰, 意愿采纳必须作为研究的解决方案整合到整个蓝图中". 这些有限理性的研究许可了在现实生活中为什么, 如何作决策的理论和在自然的环境中利用启发式的学习训练两方面的发展. 本文中的练习鼓励根据不同使用目的学习快速而简洁的启发式技巧和原则. 这也正回应了Schank的思想 "从本质上来看, 教育不是让学生们知道发生了什么, 而是让他们感受到所发生的事情. 这不容易做到. 在如今的学校教育是没有情感的, 这是一个很大的问题". 这三个案例和附加的练习问题遵守了Schank的观点. "这种教育过程最好是通过参与他们其中来实现, 也可以这样认为, 精神层面的积极讨论".