• Title/Summary/Keyword: Sequence type

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Seismic performance assessments of precast energy dissipation shear wall structures under earthquake sequence excitations

  • Zhang, Hao;Li, Chao;Wang, Zhi-Fang;Zhang, Cai-Yan
    • Earthquakes and Structures
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    • v.18 no.2
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    • pp.147-162
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    • 2020
  • This paper presents a novel precast energy dissipation shear wall (PEDSW) structure system that using mild steel dampers as dry connectors at the vertical joints to connect adjacent wall panels. Analytical studies are systematically conducted to investigate the seismic performance of the proposed PEDSW under sequence-type ground motions. During earthquake events, earthquake sequences have the potential to cause severe damage to structures and threaten life safety. To date, the damage probability of engineering structures under earthquake sequence has not been included in structural design codes. In this study, numerical simulations on single-story PEDSW are carried out to validate the feasibility and reliability of using mild steel dampers to connect the precast shear walls. The seismic responses of the PEDSW and cast-in-place shear wall (CIPSW) are comparatively studied based on nonlinear time-history analyses, and the effectiveness of the proposed high-rise PEDSW is demonstrated. Next, the foreshock-mainshock-aftershock type earthquake sequences are constructed, and the seismic response and fragility curves of the PEDSW under single mainshock and earthquake sequences are analyzed and compared. Finally, the fragility analysis of PEDSW structure under earthquake sequences is performed. The influences of scaling factor of the aftershocks (foreshocks) to the mainshocks on the fragility of the PEDSW structure under different damage states are investigated. The numerical results reveal that neglecting the effect of earthquake sequence can lead to underestimated seismic responses and fragilities, which may result in unsafe design schemes of PEDSW structures.

A retroviral insertion in the tyrosinase (TYR) gene is associated with the recessive white plumage color in the Yeonsan Ogye chicken

  • Cho, Eunjin;Kim, Minjun;Manjula, Prabuddha;Cho, Sung Hyun;Seo, Dongwon;Lee, Seung-Sook;Lee, Jun Heon
    • Journal of Animal Science and Technology
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    • v.63 no.4
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    • pp.751-758
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    • 2021
  • The recessive white (locus c) phenotype observed in chickens is associated with three alleles (recessive white c, albino ca, and red-eyed white cre) and causative mutations in the tyrosinase (TYR) gene. The recessive white mutation (c) inhibits the transcription of TYR exon 5 due to a retroviral sequence insertion in intron 4. In this study, we genotyped and sequenced the insertion in TYR intron 4 to identify the mutation causing the unusual white plumage of Yeonsan Ogye chickens, which normally have black plumage. The white chickens had a homozygous recessive white genotype that matched the sequence of the recessive white type, and the inserted sequence exhibited 98% identity with the avian leukosis virus ev-1 sequence. In comparison, brindle and normal chickens had the homozygous color genotype, and their sequences were the same as the wild-type sequence, indicating that this phenotype is derived from other mutation(s). In conclusion, white chickens have a recessive white mutation allele. Since the size of the sample used in this study was limited, further research through securing additional samples to perform validation studies is necessary. Therefore, after validation studies, a selection system for conserving the phenotypic characteristics and genetic diversity of the population could be established if additional studies to elucidate specific phenotype-related genes in Yeonsan Ogye are performed.

Differentially Expressed Genes of Potentially Allelopathic Rice in Response against Barnyardgrass

  • Junaedi, Ahmad;Jung, Woo-Suk;Chung, Ill-Min;Kim, Kwang-Ho
    • Journal of Crop Science and Biotechnology
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    • v.10 no.4
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    • pp.231-236
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    • 2007
  • Differentially expressed genes(DEG) were identified in a rice variety, Sathi, an indica type showing high allelopathic potential against barnyardgrass(Echinochloa crus-galli(L.) Beauv. var. frumentaceae). Rice plants were grown with and without barnyardgrass and total RNA was extracted from rice leaves at 45 days after seeding. DEG full-screening was performed by $GeneFishing^{TM}$ method. The differentially expressed bands were re-amplified and sequenced, then analyzed by Basic Local Alignment Search Tool(BLAST) searching for homology sequence identification. Gel electrophoresis showed nine possible genes associated with allelopathic potential in Sathi, six genes(namely DEG-1, 4, 5, 7, 8, and 9) showed higher expression, and three genes(DEG-2, 3 and 6) showed lower expression as compared to the control. cDNA sequence analysis showed that DEG-7 and DEG-9 had the same sequence. From RT PCR results, DEG-6 and DEG-7 were considered as true DEG, whereas DEG-1, 2, 3, 4, 5, and 8 were considered as putative DEG. Results from blast-n and blast-x search suggested that DEG-1 is homologous to a gene for S-adenosylmethionine synthetase, DEG-2 is homologous to a chloroplast gene for ribulose 1,5-bisphosphate carboxylase large subunit, DEG-8 is homologous to oxysterol-binding protein with an 85.7% sequence similarity, DEG-5 is homologous to histone 2B protein with a 47.9% sequence similarity, DEG-6 is homologous to nicotineamine aminotransferase with a 33.1% sequence similarity, DEG-3 has 98.8% similarity with nucleotides sequence that has 33.1% similarity with oxygen evolving complex protein in photosystem II, DEG-7 is homologous to nucleotides sequence that may relate with putative serin/threonine protein kinase and putative transposable element, and DEG-4 has 98.8% similarity with nucleotides sequence for an unknown protein.

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M2M Transformation Rules for Automatic Test Case Generation from Sequence Diagram (시퀀스 다이어그램으로부터 테스트 케이스 자동 생성을 위한 M2M(Model-to-Model) 변환 규칙)

  • Kim, Jin-a;Kim, Su Ji;Seo, Yongjin;Cheon, Eunyoung;Kim, Hyeon Soo
    • KIISE Transactions on Computing Practices
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    • v.22 no.1
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    • pp.32-37
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    • 2016
  • In model-based testing using sequence diagrams, test cases are automatically derived from the sequence diagrams. For the generation of test cases, scenarios need to be found for representing as a sequence diagram, and to extract test paths satisfying the test coverage. However, it is hard to automatically extract test paths from the sequence diagram because a sequence diagram represents loop, opt, and alt information using CombinedFragments. To resolve this problem, we propose a transformation process that transforms a sequence diagram into an activity diagram which represents scenarios as a type of control flows. In addition, we generate test cases from the activity diagram by applying a test coverage concept. Finally, we present a case study for test cases generation from a sequence diagram.

A study on sequencing of Mixed Model Assembly Line for increasing productivity (혼합모델조립라인의 생산성 제고를 위한 작업순서 결정)

  • 최종열
    • Korean Management Science Review
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    • v.13 no.2
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    • pp.25-48
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    • 1996
  • Mixed Model Assembly Lines (MMALs) are increasingly used to produce differentiated products on a single assembly line without work-in-process storage, Usually, a typical MMAL consists of a number of (1) stations doing exactly the same operation on every job, (2) stations involving operations with different choices, and (3) stations offering operations that are not performed on every job, or that are performed on every job but with many options. For stations of the first type there is no sequencing problem at all. However, for the second type a set-up cost is incurred each time the operation switches from one choice to another. At the third type of stations, different models, requring different amounts and choices of assembly work, creates an uneven flow of work along the line and variations in the work load at these stations. When a subsequence of jobs requires more work load than the station can handle, it is necessary to help the operations at the station or to complete the work elsewhere. Therefore, a schedule which minimize the sum of set-up cost and utility work cost is desired. So this study has developed Fixed Random Ordering Rule (FROR), Fixed Ascending Ordering Rule (FAOR), Fixed Descending Ordering Rule, and Extended NHR (ENHR). ENHR is to choose optimal color ordering of each batch with NHR, and to decide job sequence of the batch with it, too. As the result of experiments, ENHR was the best heuristic algorithm. NHR is a new heuristic rule in which only the minimum addition of violations from both partial sequence and unassigned sequence at every branch could be considered. And this is a heuristic sequencing rule for the third type of stations at MMAL. This study developed one more heuristic algorithm to test the performance of NHR, which is named as Practical Heuristic Rule (PHR).

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Cloning and Molecular Characterization of ${\beta}$-1,3-Glucan Synthase from Sparassis crispa

  • Yang, Yun Hui;Kang, Hyeon-Woo;Ro, Hyeon-Su
    • Mycobiology
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    • v.42 no.2
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    • pp.167-173
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    • 2014
  • A ${\beta}$-glucan synthase gene was isolated from the genomic DNA of polypore mushroom Sparassis crispa, which reportedly produces unusually high amount of soluble ${\beta}$-1,3-glucan (${\beta}$-glucan). Sequencing and subsequent open reading frame analysis of the isolated gene revealed that the gene (5,502 bp) consisted of 10 exons separated by nine introns. The predicted mRNA encoded a ${\beta}$-glucan synthase protein, consisting of 1,576 amino acid residues. Comparison of the predicted protein sequence with multiple fungal ${\beta}$-glucan synthases estimated that the isolated gene contained a complete N-terminus but was lacking approximately 70 amino acid residues in the C-terminus. Fungal ${\beta}$-glucan synthases are integral membrane proteins, containing the two catalytic and two transmembrane domains. The lacking C-terminal part of S. crispa ${\beta}$-glucan synthase was estimated to include catalytically insignificant transmembrane ${\alpha}$-helices and loops. Sequence analysis of 101 fungal ${\beta}$-glucan synthases, obtained from public databases, revealed that the ${\beta}$-glucan synthases with various fungal origins were categorized into corresponding fungal groups in the classification system. Interestingly, mushrooms belonging to the class Agaricomycetes were found to contain two distinct types (Type I and II) of ${\beta}$-glucan synthases with the type-specific sequence signatures in the loop regions. S. crispa ${\beta}$-glucan synthase in this study belonged to Type II family, meaning Type I ${\beta}$-glucan synthase is expected to be discovered in S. crispa. The high productivity of soluble ${\beta}$-glucan was not explained but detailed biochemical studies on the catalytic loop domain in the S. crispa ${\beta}$-glucan synthase will provide better explanations.