• Journal of Microbiology and Biotechnology
• /
• 제26권9호
• /
• pp.1643-1649
• /
• 2016

The aims of this study were to characterize the molecular epidemiological profiles of CTX-M-producing uropathogenic Escherichia coli isolates from a tertiary hospital in Daejeon, Korea, and to investigate the genetic diversity and compare the prevalence of sequence types (STs) in different areas. Extended spectrum β-lactamase-producing E. coli strains isolated from urine were analyzed for CTX-M, integrons, and insertion sequence common regions (ISCRs) by PCR and sequencing. Multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), phylogenetic analysis, and rep-PCR were also used for molecular typing of the isolates. Of 80 CTX-M producers, 31 and 46 expressed CTX-M-15 and CTX-M-14, respectively. MLST analysis indicated that the most prevalent ST was ST131 (n = 34, 42.5%), followed by ST38 (n = 22, 27.5%), ST405 (n = 8, 10.0%), and ST69 (n = 6, 7.5%). Most CTX-M producers harbored class 1 integrons. ST131 strains belonged to phylogenetic group B2 and showed identical rep-PCR patterns, whereas ST69, ST38, and ST405 strains belonged to phylogenetic group D; the ST38 and ST405 strains displayed the same rep-PCR pattern, respectively. ST131 and ST38 isolates showed 21 and 19 distinct types, respectively, by PFGE. In Daejeon, D-ST38 CTX-M-14 producers were relatively more prevalent than in other countries and Korean cities. Our results indicate that CTX-M-producing E. coli isolates belonged mostly to ST131 or ST38 and were more related to hospital-onset than to community-onset infections and that the bla_CTX-M gene may vary according to the ST.

• Journal of Microbiology and Biotechnology
• /
• 제19권8호
• /
• pp.774-780
• /
• 2009

Flammulina velutipes is a popular edible basidiomycete mushroom found in East Asia and is commonly known as winter mushroom. Mushroom development showing dramatic morphological changes by different environmental factors is scientifically and commercially interesting. To create a genetic database and isolate genes regulated during mushroom development, cDNA libraries were constructed from three developmental stages of mycelium, primordium, and fruit body in F. velutipes. We generated a total of 5,431 expressed sequence tags (ESTs) from randomly selected clones from the three cDNA libraries. Of these, 3,332 different unique genes (unigenes) were consistent with 2,442 (73%) singlets and 890 (27%) contigs. This corresponds to a redundancy of 39%. Using a homology search in the gene ontology database, the EST unigenes were classified into the three categories of molecular function (28%), biological process (29%), and cellular component (6%). Comparative analysis found great variations in the unigene expression pattern among the three different unigene sets generated from the cDNA libraries of mycelium, primordium, and fruit body. The 19-34% of total unigenes were unique to each unigene set and only 3% were shared among all three unigene sets. The unique and common representation in F. velutipes unigenes from the three different cDNA libraries suggests great differential gene expression profiles during the different developmental stages of F. velutipes mushroom.

• 한국초등과학교육학회지:초등과학교육
• /
• 제27권4호
• /
• pp.356-370
• /
• 2008

The study aims to explore sequence of earth science content in elementary school science curriculum in Korea and the U.S.. The analysis is focused on a) general content structure of earth science part; b) concept relationship between grades in the specific field of 'geology'; c) longitudinal connection of concepts and content in 'geology.' The findings are as follows. First, earth science curriculum content in Korea is structured according to sub-scientific disciplines centering on not science concepts but topics or inquiry activities whereas the U.S. curricular content is organized through integrative earth science topics with basic concepts and sub-concepts. Second, it is a common feature that basic concepts are interrelated to sub-concepts in all grades in both countries. However, basic concepts are scattered all over the grades, presented in a linear pattern in Korea while those are provided together in 3rd grade and repeated with extended concepts in a spiral structure in the U.S.. Last, it is not clear how concepts and content are longitudinally connected between grades in Korean curriculum. On the contrary, concepts and content in the U.S. curriculum have a strong longitudinal connection between grades with conceptual hierarchy. Such results indicate that Korean elementary school science curriculum would limit students' comprehensive understanding of science concepts through grades. The study suggests Korean science content should strengthen interrelationship among concepts as well as longitudinal connection between grades, in order to achieve the ultimate goal of science education, 'scientific literacy'.

• 한국미생물·생명공학회지
• /
• 제47권1호
• /
• pp.25-33
• /
• 2019

Lactobacillus rhamnosus BFE5264, isolated from a Maasai fermented milk product ("kule naoto"), was previously shown to exhibit bile acid resistance, cholesterol assimilation, and adhesion to HT29-MTX cells in vitro. In this study, we re-annotated and analyzed the previously reported complete genome sequence of strain BFE5264. The genome consists of a circular chromosome of 3,086,152 bp and a putative plasmid, which is the largest one identified among L. rhamnosus strains. Among the 2,883 predicted protein-coding genes, those with carbohydrate-related functions were the most abundant. Genome analysis of strain BFE5264 revealed two consecutive CRISPR regions and no known virulence factors or antimicrobial resistance genes. In addition, previously known highly variable regions in the genomes of L. rhamnosus strains were also evident in strain BFE5264. Pairwise comparison with the most studied probiotic strain L. rhamnosus GG revealed strain BFE5264-specific deletions, probably due to insertion sequence-mediated recombination. The latter was associated with loss of the spaCBA pilin gene cluster and exopolysaccharide biosynthetic genes. Comparative genomic analysis of the sequences from all available L. rhamnosus strains revealed that they were clustered into two groups, being within the same species boundary based on the average nucleotide identities. Strain BFE5264 had a sister group relationship with the group that contained strain GG, but neither ANI-based hierarchical clustering nor core-gene-based phylogenetic tree construction showed a clear distinctive pattern associated with the isolation source, implying that the genotype alone cannot account for their ecological niches. These results provide insights into the probiotic mechanisms of strain BFE5264 at the genomic level.

• 식물병연구
• /
• 제11권2호
• /
• pp.140-145
• /
• 2005

제주지역의 감자 줄기검은병징으로부터 분리한 12균주의 유전적 특성을 분석하기 위해 Eca-specific PCR, PCR-RFLP, ERIC-PCR을 실시하여 그 결과를 E. carotovora대조균들과 비교하였다. Eca-specific PCR 결과 Eca 대조균들은 특이적 밴드를 형성한 반면, 줄기검은병균은 특이적 밴드를 형성하지 않았다. 또한, pel유전자의 RFLP분석 결과 줄기검은병균은 pattern 2를 나타내었으나, Eca 균주는 pattern 3을 나타내어 Eca와는 다른 특성을 보여주었다. 16S rDNA의 RFLP분석결과 이번 실험에 이용된 대부분의 균주가 pattern 1을 나타냈지만, 12개의 줄기검은병균 중 11개의 균이 pattern 2을 나타내어 Ecc와도 다른 특성을 보여주었다. 제주지역의 무름병징과 줄기검은병징을 나타내는 균주들의 유전적 관계를 분석하기 위해 ERIC-PCR을 실시한 결과 줄기검은병균들은 특이적 밴드를 형성하였으며, 서로 높은 유연관계를 보여주었다. 따라서 제주지역의 줄기검은병징으로부터 분리한 균주들은 Eca, Ecc균들과는 다른 특성을 가지고 있음을 알 수 있었다.

• BMB Reports
• /
• 제42권9호
• /
• pp.611-616
• /
• 2009

We investigated the expression pattern of dehydration responsive element-binding protein-3 in tomato under different abiotic stresses. Full length LeDREB3 cDNA was isolated from tomato plant, followed by phylogenetic analysis based on deduced amino acid sequences that revealed significant sequence similarity to DREB proteins belonging to diverse families of plant species. Southern blot analysis showed duplicate copies of LeDREB3 in the tomato genome while organ-specific expression profiling indicated constitutive expression of LeDREB3 in all tested organs, which was particularly strong in flower. LeDREB3 expression was significantly induced by Nacl, drought, low temperature and $H_2O_2$. Moreover, LeDREB3 was slightly regulated by treatment with ABA and MV. These observations suggest that the LeDREB3 gene may be involved in the response of the tomato plant to stress.

• 한국정보시스템학회:학술대회논문집
• /
• 한국정보시스템학회 2003년도 추계학술대회
• /
• pp.41-54
• /
• 2003

This study examines group process patterns when Instant Messaging is used for decision-making, and examines how these patterns are associated with creative solutions to problems. Our research suggests that certain communication behavior of a group, when appropriately organized, can enhance creative production of outcomes. A qualitative analysis is conducted on communication patterns based on text-based conversation protocols. Specifically, this research tries to extend existing studies on group-work by focusing on the interactive communication process among participants. Study results include that the production of creative outcome depends on the temporal sequence of discussion pattern among group members. (1) Appropriate control of the discussion process is essential to obtain a high level of performance. (2) It is also important to set up discussion rules and rules for the use of communication medium in the early stages of the discussion . (3) Active participants use various protocol types while less-active members rely mainly on 'cognitive' protocols.

• 한국지능시스템학회:학술대회논문집
• /
• 한국퍼지및지능시스템학회 2003년도 ISIS 2003
• /
• pp.224-227
• /
• 2003

The Intrsuion Detecion Systems(IDS) are required the accuracy, the adaptability, and the expansion in the information society to be changed quickly. Also, it is required the more structured, and intelligent IDS to protect the resource which is important and maintains a secret in the complicated network environment. The research has the purpose to build the model for the intelligent IDS, which creates the intrusion patterns. The intrusion pattern has extracted from the vast amount of data. To manage the large size of data accurately and efficiently, the link analysis and sequence analysis among the data mining techniqes are used to build the model creating the intrusion patterns. The model is consist of "Time based Traffic Model", "Host based Traffic Model", and "Content Model", which is produced the different intrusion patterns with each model. The model can be created the stable patterns efficiently. That is, we can build the intrusion detection model based on the intelligent systems. The rules prodeuced by the model become the rule to be represented the intrusion data, and classify the normal and abnormal users. The data to be used are KDD audit data.

• 한국경영정보학회:학술대회논문집
• /
• 한국경영정보학회 2008년도 춘계학술대회
• /
• pp.153-170
• /
• 2008

Customers' needs change every moment. Profitability of stores can't be increased anymore with an existing standardized chain store management. Accordingly, a personalized store management tool needs through prediction of customers' preference. In this study, we propose a recommending procedure using dynamic customers' preference by analyzing the transaction database. We utilize self-organizing map algorithm and association rule mining which are applied to cluster the chain stores and explore purchase sequence of customers. We demonstrate that the proposed methodology makes an effect on recommendation of products in the market which is characterized by a fast fashion and a short product life cycle.

• Journal of Plant Biology
• /
• 제38권2호
• /
• pp.137-141
• /
• 1995

We isolated a cDNA clone, BLSC1, encoding 5' exon of ATP synthase CF0 subunit I from broccoli. BLSC1 is 285 nucleotides long which consists of a 5' noncoding region of 34 nucleotides, a 5' exon of 145 nucleotides and an intron of 106 nucleotides. The 5' exon codes for 48 amino acids which reveals mostly hydrophobic. The amino acid sequence deduced from BLSC1 shares 83%, 83% and 91% identities with the genes coding for atpF from wheat, rice and spinach, respectively. Genomic Southern blot analysis for BLSC1 showed a typically strong signal for a gene located in the chloroplast genome. Northern blot analysis identified three major classes of transcripts showing strong positive signals in the leaves, but only trace amounts of the transcripts were identified in the other organs like stems, flowr buds and roots.