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Fluorescence Quenching Causes Systematic Dye Bias in Microarray Experiments Using Cyanine Dye

  • Jeon, Ho-Sang;Choi, Sang-Dun
    • Genomics & Informatics
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    • v.5 no.3
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    • pp.113-117
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    • 2007
  • The development of microarray technology has facilitated the understanding of gene expression profiles. Despite its convenience, the cause of dye-bias that confounds data interpretation in dual-color DNA microarray experiments is not well known. In order to economize time and money, it is necessary to identify the cause of dye bias, since designing dye-swaps to reduce the dye-specific bias tends to be very expensive. Hence, we sought to determine the reliable cause of systematic dye bias after treating murine macrophage RAW 264.7 cells with 2-keto-3-deoxyoctonate (KDO), interferon-beta $(IFN-{\beta})$, and 8-bromoadenosine (8-BR). To find the cause of systematic dye bias from the point of view of fluorescence quenching, we examined the correlation between systematic dye bias and the proportion of each nucleotide in mRNA and oligonucleotide probe sequence. Cy3-dye bias was highly correlated with the proportion of adenines. Our results support the fact that systematic dye bias is affected by fluorescence quenching of each feature. In addition, we also found that the strength of fluorescence quenching is based on not only dye-dye interactions but also dye-nucleotide interactions as well.

Cinematic Language for Novel Adaptations : A Case Study of (소설의 영화화를 위한 영상 언어 연구 : <키리시마가 동아리활동 그만둔대>를 중심으로)

  • Hwang, Woo-Hyun
    • The Journal of the Korea Contents Association
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    • v.17 no.1
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    • pp.634-661
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    • 2017
  • This study examines the procedure of successful novel adaptation. It is well known from precedent studies that narrative structure of novels should change forms to suit the media characteristics of films. But, the changing forms of narrative structure is not a sufficient condition of successful novel adaptation, but a necessary condition. A successful adaptation could be completed with filmic expressions on the presented narrative structure. The core of filmic expression is cinematic language which means the composition and array of image and sound. The novel, Kirishma Thing deals with everyday life of high school students and it consists of six stories which are narrated by one student each in first person point of view. The film, Kirishma Thing implemented a different strategy. It reveals the same events several times to show many characters over in each person's point of view in the first half. In the second half, all the characters gathers at the rooftop of the school to have an unilinear narrative structure over one event. This film utilize all kinds of cinematic language to achieve these structures including the widescreen aspect ratio which exposes as many characters as possible in one shot, picture composition which shows the same event in a different point of view, contrast in lighting and music which differentiates and empowers the last sequence of the film.

The In/Out Structure : Interpretation of the Korean Architectural Space by Structuralism (안팍구조(構造)-한국건축공간(韓國建築空間)의 구조주의적(構造主義的) 해석(解析))

  • Lee, Hee-Bong
    • Journal of architectural history
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    • v.1 no.2 s.2
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    • pp.172-182
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    • 1992
  • The binary classification, Yin & Yang, can be shown in our culture. Yin & Yang thinking reveals the Up/Down (Principal/Subordinate) relation in space, and Before/After relation in time, and it shows the sequence of Contrast${\to}$Reconciliation & Unification${\to}$Contrast. For example, a follower is Yin against King, while Yang as a father against his son in our trational cognitive structure. With Left/Right, Front/Rear, and Upper/Lower, In/Out division is a basic body-space term to grasp the space position. In the traditional Korean house, when we go out from the deepest 'In', Anbang to Daechung, we may call Anbang 'In' and Daechung 'Out'. When from Daechung to Anmadang reversely, we call Daechung 'In' and Anmadang 'Out'. Namely, Daechung is both 'In' and 'Out'. This is a fallacy logic in view of western basic conception. 'The In/Out Structure, system of a series of inner spaces, is defined as a transformation of cognitive structure of Yin & Yang thinking to the Korean architectural space. The space structure is one of the important deep structure in Korean society.' The concept of the space structure, apperred in east Asia, can make humane space, for it is not a physical 'type' of typepology but related to cognitive structure of human thinking, The structure is applicable to space design in modern society, for the cognitive structure exists in living culture by transformation.

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Enhanced Technique for Performance in Real Time Systems (실시간 시스템에서 성능 향상 기법)

  • Kim, Myung Jun
    • Journal of Information Technology Services
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    • v.16 no.3
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    • pp.103-111
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    • 2017
  • The real time scheduling is a key research area in high performance computing and has been a source of challenging problems. A periodic task is an infinite sequence of task instance where each job of a task comes in a regular period. The RMS (Rate Monotonic Scheduling) algorithm has the advantage of a strong theoretical foundation and holds out the promise of reducing the need for exhaustive testing of the scheduling. Many real-time systems built in the past based their scheduling on the Cyclic Executive Model because it produces predictable schedules which facilitate exhaustive testing. In this work we propose hybrid scheduling method which combines features of both of these scheduling algorithms. The original rate monotonic scheduling algorithm didn't consider the uniform sampling tasks in the real time systems. We have enumerated some issues when the RMS is applied to our hybrid scheduling method. We found the scheduling bound for the hard real-time systems which include the uniform sampling tasks. The suggested hybrid scheduling algorithm turns out to have some advantages from the point of view of the real time system designer, and is particularly useful in the context of large critical systems. Our algorithm can be useful for real time system designer who must guarantee the hard real time tasks.

A Flow Characteristics for a Separation Behavior of Two-body Vehicle (비행 조건에 따른 비행체 단분리의 주위 유동장 해석)

  • Park, Geunhong;Kim, Kiun
    • Proceedings of the Korean Society of Propulsion Engineers Conference
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    • 2017.05a
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    • pp.266-267
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    • 2017
  • A numerical investigation of the stage separation behavior of two-body vehicle focusing on its flow characteristics were carried out. For this simulation, separation of a booster from vehicle was modeled by a chimera grid system and calculated by using commercial code, CFD-FASTRAN$^{TM}$. Consideration of a spring force, gravity and relative acceleration of a booster was the essential factor that simulates the realistic situation. In this study, It was validated that the booster separation time decreases with increase in flight mach number and angle of attack. In view of the results so far achieved, it was expected that the dynamics modeling and boundary condition set up applied in this study will be helpful in a estimation of a safe stage separation and event sequence of flight test.

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The CTIO 4m UBVI & $H{\alpha}$ photometry and spatial variation of the reddening law in the ${\eta}$ Carina nebula

  • Hur, Hyeonoh;Sung, Hwan Kyung;Lim, Beomdu;Chun, Moo-Young;Sohn, Sangmo Tony
    • The Bulletin of The Korean Astronomical Society
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    • v.40 no.1
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    • pp.71.3-71.3
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    • 2015
  • Deep UBVI and $H{\alpha}$ photometry of the ${\eta}$ Carina nebula, one of the brightest nebulae on the sky, was obtained with the CTIO 4m telescope and MOSAIC II CCD Camera to determine the initial mass function down to low-mass (~1 M¤) stars. We modified the spatial variation coefficients in transformation relations of the MOSAIC II CCD. From the cross-identification of optical sources with previous surveys in X-ray, near-infrared, and mid-infrared, a clear PMS sequence is revealed in the optical color-magnitude diagrams down to V=23 mag. Our previous SSO 1m UBVI data for Trumpler 14 (Tr 14) and Trumpler 16 (Tr 16) region, and additional SSO 1m UBVI data for Trumpler 15 (Tr 15) region were combined with the CTIO 4m data to re-examine the reddening law and distance of the young open clusters in the ${\eta}$ Carina nebula. From the new photometric data for Tr 15 region, we report that RV[=AV/E(B-V)], the total-to-selective extinction ratio, decreases from southern part of the nebula (Tr 14 and Tr 16) to northern part (Tr 15) in our field of view.

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Analysis of the Sexual Health Education Curriculum of Nursing Schools in Korea using Posner's Theory (한국 간호교육기관의 성 건강 이론 교육과정 분석)

  • Kim, Hyun-Kyoung
    • Korean Journal of Adult Nursing
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    • v.26 no.2
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    • pp.223-233
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    • 2014
  • Purpose: This study was aimed to analyze the sexual health curriculum for the nursing baccalaureate and associate's degrees in Korea. The curriculum proper based on Posner's theory presented the analysis of purpose, content, organization, and underlying assumption. Methods: This study was conducted with sexual health education guidelines, nursing practice standards, 181 curriculums, and teaching materials. Data were collected through literature, online homepage from 181 nursing school, and textbooks from July to September, 2013. Data were analyzed using percentage and mean with SPSS 12.0. Results: The purposes were mostly included in the low grade cognitive learning domain. The contents included 20 key elements among 22, so the scope was not inclusive. There was an unbalance between content's depth and scope, because total mean credit of sexual health nursing education was only 19.81 hours. The spiral structure of organization showed continuity, sequence, and integration with international standards. The interdisciplinary integration and transcultural value were advantages of the curriculum. Conclusion: This study provided a view on understanding sexual health nursing curriculum and implication for advanced education. The proclaiming of the standard and concept mapping of sexual health curriculum may contribute to the curriculum development for the advanced nursing.

Autonomous vision-based damage chronology for spatiotemporal condition assessment of civil infrastructure using unmanned aerial vehicle

  • Mondal, Tarutal Ghosh;Jahanshahi, Mohammad R.
    • Smart Structures and Systems
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    • v.25 no.6
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    • pp.733-749
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    • 2020
  • This study presents a computer vision-based approach for representing time evolution of structural damages leveraging a database of inspection images. Spatially incoherent but temporally sorted archival images captured by robotic cameras are exploited to represent the damage evolution over a long period of time. An access to a sequence of time-stamped inspection data recording the damage growth dynamics is premised to this end. Identification of a structural defect in the most recent inspection data set triggers an exhaustive search into the images collected during the previous inspections looking for correspondences based on spatial proximity. This is followed by a view synthesis from multiple candidate images resulting in a single reconstruction for each inspection round. Cracks on concrete surface are used as a case study to demonstrate the feasibility of this approach. Once the chronology is established, the damage severity is quantified at various levels of time scale documenting its progression through time. The proposed scheme enables the prediction of damage severity at a future point in time providing a scope for preemptive measures against imminent structural failure. On the whole, it is believed that the present study will immensely benefit the structural inspectors by introducing the time dimension into the autonomous condition assessment pipeline.

User Profile Based Seamless Framework under HTTP Adaptive Streaming Environment (HTTP Streaming 환경에서 User Profile 기반 Seamless Framework 제공방법)

  • Kim, Jung-Han;Lee, Jang-Won;Kim, Kyu-Heon;Suh, Doug-Young
    • Journal of Broadcast Engineering
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    • v.16 no.1
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    • pp.155-173
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    • 2011
  • Recently, with the digitalization of a broadcasting system and the development of a communication technology, the existing trend of consuming media contents throughout the fixed-displayer and the dedicated channel is being changed. The existing user only could consume media contents under limited time and places because of the fixed-displayer and the dedicated channel. However, with advent of the IP-based terminals and HTTP adaptive streaming which transfer the media sequence according to the user's transmission condition, users become possible to enjoy the media content anytime anywhere. As the result of the alteration of the broadcasting surrounding, users can enjoy the media content while changing his terminals according to their preferences and circumstances. However, in case that users try to consume consecutively the content from last view-point ended in the previous terminal under current HTTP adaptive streaming environment, a user has to remember the last view-point, and then has to apply the view-point to the changed terminal. Thus, for solving this problem, this paper defines "User Profile" for describing the metadata for the chained content consume environment between the terminals. Also, for proving the proposed method, this paper try to demonstrate the proposed method throughout the realization of the system based on Smooth Streaming from Microsoft.

A Phenomenological Study on the Information Technology Acceptance of the Korean Baby Boomer Generation

  • Kim, Sohyun;Brady, John Thomas
    • International Journal of Advanced Culture Technology
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    • v.7 no.4
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    • pp.172-186
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    • 2019
  • As ICT technology develops rapidly and social and consumer interactions require more knowledge of the technology, the interest for baby boomers' ICT technology acceptance is increasing as they age. However, previous studies have limitations as they lack a systematic and in-depth understanding of this phenomenon in which the emerging elderly population embraces emerging technology. To overcome this limitation, this study carried out a phenomenological study on baby boomers' ICT technology acceptance. In addition, since existing studies have just simply listed variables or proved the relationship between a few variables, this study aimed to find the relationship between variables by analyzing the data obtained through semi-structured interviews. Through this procedure, it was aimed to construct conceptual model which illustrates the relationships between variables so that understanding the phenomenon of baby boomers' acceptance of ICT technology with a holistic view could be possible. The ten variables suggested by Lee & Coughlin (2015) that cover multiple sides of baby boomers' technology acceptance were used. As a result, we found that there is a sequential relationship between these variables and different agents can be related to each variable in sequence. Also, from an ecological perspective, we analyzed baby boomers and their surroundings to find agents involved in this phenomenon.