• Journal of Microbiology
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• v.33 no.3
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• pp.260-266
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• 1995

Hepatitis C virus (HCV) has been considered as a mojor causative agent of post-transfusion related non-A, non-B hepatitis. In this study, the cDNA sequence of NS4 region of HCV (HCV-S) obtained from a Korean patient's plasms was determined. Comparative nucleotide sequence analysis between to type II. 67.2% homology to type III, and 66.4% homology to type IV. The putative amino acid sequence homologies to types I, II, III, and IV were 82.8-84.7%, 92.5-95.1%. 72.5, and 71.1%, respectively. This data strongly suggests that HCV-S should be classified as type II. Significant similarities of hydrophobicity profiles and putative transmembranous domains were found in HCV-S and four major prototypes, indicating that the protein structure is similar in spite of the heterogeneities of intertype homologies at the level of the psrimary nucleotide and amino acid sequences.

• Applied Biological Chemistry
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• v.36 no.3
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• pp.208-214
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• 1993

To investigate the gene rearrangement via short repeated sequences in chloroplast DNA, the pattern of heterologous gene clusters containing the 151 bp repeated sequence with the development of plastid was compared in rice and the homologous gene clusters from various plant sources were searched for comparative analysis. Southern blot analysis of rice DNA using rp12 gene containing 151 bp repeated sequence as a probe showed the presence of heterologous gene clusters. Such heterologous gene clusters varied with the development of plastid. Also it was observed that the heterologous gene clusters were observed in all of the rice cultivars used in this work. Finally the comparative analysis of DNA sequence of the homologous gene clusters from various plants showed the evolutionary gene rearragngement via short repeated sequence among plants. These results suggest the possible relationship between the plastid development and gene rearrangement through short repeated sequences.

• Parasites, Hosts and Diseases
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• v.53 no.3
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• pp.345-348
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• 2015

Toxoplasma gondii is a eukaryotic parasite of the phylum Apicomplexa, which infects all warm-blood animals, including humans. In the present study, we examined sequence variation in dense granule 20 (GRA20) genes among T. gondii isolates collected from different hosts and geographical regions worldwide. The complete GRA20 genes were amplified from 16 T. gondii isolates using PCR, sequence were analyzed, and phylogenetic reconstruction was analyzed by maximum parsimony (MP) and maximum likelihood (ML) methods. The results showed that the complete GRA20 gene sequence was 1,586 bp in length among all the isolates used in this study, and the sequence variations in nucleotides were 0-7.9% among all strains. However, removing the type III strains (CTG, VEG), the sequence variations became very low, only 0-0.7%. These results indicated that the GRA20 sequence in type III was more divergence. Phylogenetic analysis of GRA20 sequences using MP and ML methods can differentiate 2 major clonal lineage types (type I and type III) into their respective clusters, indicating the GRA20 gene may represent a novel genetic marker for intraspecific phylogenetic analyses of T. gondii.

• Asian-Australasian Journal of Animal Sciences
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• v.6 no.4
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• pp.541-547
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• 1993

A phage clone containing the partial ${\alpha}_{S1}$-casein gene was isolated from a bovine genomic library by using mixed probes of ovine ${\alpha}_{S1}$-, ${\beta}$- and ${\kappa}$-casein cDNAs. Restriction enzyme mapping analysis for 14.6 kb revealed that the map was in conflict with the report of Meade et al. (1990), especially in the 3'-end fragment. Sequence analysis of 12.6 kb revealed a high AT/GC ratio (1.64); we have identified eight exon sequences according to the bovine ${\alpha}_{S1}$-casein cDNA sequence. The same exon/intron splice junction sequence was observed between these exons. We suggest that the bovine ${\alpha}_{S1}$-casein gene night contain a minimum of 18 exons and the full length is approximately 18-19 kb.

• International Journal of Industrial Entomology and Biomaterials
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• v.3 no.2
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• pp.191-199
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• 2001

We have constructed a cDNA library from the whole body of the spider, Araneus ventricosus. Sequence analysis of randomly selected cDNA clones was performed to obtain genetic information on the spider A. ventricosus, of which genetic information is currently not available. We have partially sequenced randomly selected 385 clones of the cDNA library constructed from A. ventricosus. This expressed sequence tags (EST) analysis revealed 383 genes had high homologies to known genes in GenBank. In this report, 241 independent genes were analyzed in detail.

• Food Science and Biotechnology
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• v.27 no.6
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• pp.1755-1760
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• 2018

The objective of this study was to perform genetic diversity analysis of 13 strains isolated from South Korean foods by multilocus sequence typing (MLST). For typing, seven housekeeping loci (atpA, dnaA, dnaK, gyrB, pheS, pyrG, and rpoA) were selected, amplified and analyzed. Fifty-one polymorphic sites varying from 1 to 22 in each species were identified. Thirteen sequence types were generated with allele numbers ranged from 2 to 10. The overall relationship between strains was assessed by unweighted pair group method with arithmetic mean dendrogram and minimum spanning tree. In addition, combined spits tree analysis revealed intragenic recombination. No clear relationship was observed between the isolation sources and strains. The developed MLST scheme enhanced our knowledge of the population diversity of Leu. citreum strains and will be used further for the selection of industrially important strain.

• Annual Conference on Human and Language Technology
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• 2020.10a
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• pp.414-417
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• 2020

최근 한국어 형태소 분석 및 품사 태깅에 관한 연구는 주로 표층형에 대해 형태소 분리와 품사 태깅을 먼저하고, 추가 언어자원을 사용하여 후처리로 형태소 원형과 품사를 복원해왔다. 본 연구에서는 형태소 분석 및 품사 태깅을 두 단계로 나누어, Sequence-to-Sequence를 활용하여 형태소 원형 복원을 먼저 하고, 최근 자연어처리의 다양한 분야에서 우수한 성능을 보이는 BERT를 활용하여 형태소 분리 및 품사 태깅을 하였다. 본 논문에서는 두 단계를 파이프라인으로 연결하였고, 제안하는 형태소 분석 및 품사 태깅 파이프라인 모델은 음절 정확도가 98.39%, 형태소 정확도 98.27%, 어절 정확도 96.31%의 성능을 보였다.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.27 no.1
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• pp.91-101
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• 2017

API call sequence analysis is a kind of analysis using API call information extracted in target program. Compared to other techniques, this is advantageous as it can characterize the behavior of the target. However, existing API call sequence analysis has an issue of identifying same characteristics to different function during the analysis. To resolve the identification issue and improve performance of analysis, this study includes the method of API abstraction technique in addition to existing analysis. From there on, similarity between target programs is computed and clustered into similar types by applying LSH to abstracted API call sequence from analyzed target. Thus, this study can attribute in improving the accuracy of the malware analysis based on discovered information on the types of malware identified.

• Structural Monitoring and Maintenance
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• v.5 no.2
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• pp.173-187
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• 2018

Performance evaluation of RC frame building by nonlinear static pushover analysis that accounts for elastic and post elastic behavior is becoming very popular as a valid decision making tool in seismic hazard resistant designs. Available literature suggests great amount of interest has shown by researchers in suggesting refinements to geometric and material modelling to bridge the gap between analytical predictions and observed performances. Notwithstanding the attempts gaps still exists. Sequence of plastic hinge formation which has great influence on pushover analysis results is an area less investigated. This paper attempts to highlight the importance of hinge sequence considerations to make analysis results more meaningful. Variation in analysis results due to different hinge sequences have been quantified, compared and bounds on analysis results have been presented.

• The Journal of the Korean Society for Microbiology
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• v.34 no.6
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• pp.561-571
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• 1999

Diagnosis of mycobacterial infection is dependent upon the isolation and identification of causative agents. The procedures involved are time consuming and technically demanding. To improve the laborious identification process mycobacterial systematics supported by gene analysis is feasible, being particularly useful for slowly growing or uncultivable mycobacteria. To complement genetic analysis for the differentiation and identification of mycobacterial species, an alternative marker gene, rpoB encoding the ${\beta}$ subunit of RNA polymerase, was investigated. rpoB DNAs (342 bp) were amplified from 52 reference strains of mycobacteria including Mycobacterium tuberculosis H37Rv (ATCC 27294) and clinical isolates by the PCR. The nucleotide sequences were directly determined (306 bp) and aligned using the multiple alignment algorithm in the MegAlign package (DNASTAR) and MEGA program. A phylogenetic tree was constructed with a neighborhood joining method. Comparative sequence analysis of rpoB DNA provided the basis for species differentiation. By being grouped into species-specific clusters with low sequence divergence among strains belonging to same species, all the clinical isolates could be easily identified. Furthermore RFLP analysis enabled rapid identification of clinical isolates.