• 한국CDE학회논문집
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• 제16권1호
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• pp.61-72
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• 2011

As the customer's needs for diverse colors, the process of bus painting shop is becoming more and more complex. In particular, frequent changes of paint colors in each spray process require the cleaning of the painting nozzles and setting them up for the new paints, which results in increased time and cost and reduced production. This also increases environmental influences. This paper proposes, as a solution to this issue, the use of color selection field (CSF). CSF is physically a system of conveyors that are used to rearrange objects in a flow line. CSF is proposed, in this paper, to be introduced right before the spray process so that buses are better grouped according to their desired colors. Various parameters of the CSF, such as the number of conveyors and algorithms to determine the rearranged sequence of buses, have been determined by running simulations using the digital manufacturing technology, and investigating the simulation results. As an outcome of the proposed solution, the CSF system is expected to reduce approximately 30% of the nozzle cleaning cost for a Korean motor company case, which will not only benefit the company economically, but will also benefit the environment.

• 정보처리학회논문지D
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• 제14D권5호
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• pp.459-470
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• 2007

데이터 스트림은 빠르게 연속적으로 발생하는 무제한의 데이터 튜플의 집합이다. 이러한 데이터 스트림에 대한 질의 처리 또한 연속적이고 신속해야 하며 엄격한 시공간적 제약이 요구된다. 대부분의 데이터 스트림 관리시스템(DSMS)에서는 시공간적 제약사항을 효과적으로 지키기 위해서 등록된 연속 질의들의 선택 조건(selection predicate)들을 그룹화하거나 색인처리 한다. 본 논문에서는 연속 질의들의 선택 조건들을 속성별로 그룹화한 새로운 구조체인 속성 선택체(Attribute Selection Construct)를 제안한다. 속성 선택체에는 해당 속성이 특정 질의조건에 사용되는지 여부, 부분적으로 미리 계산된 질의결과 정보, 그리고 해당 속성의 선택률 통계 등 효율적인 질의 처리를 위한 유용한 정보들이 포함된다. 또한, 대상 질의집합을 구현한 속성 선택체들 간의 처리 순서는 전체적인 질의성능에 많은 영향을 미칠 수 있기 때문에 효과적으로 속성 선택체 처리 순서를 결정할 수 있는 전략도 함께 제안된다. 마지막으로, 기존의 방법들이 포함된 다양한 실험을 통하여 제안된 방법론의 성능을 여러 각도에서 비교 검증한다.

• The Korean Journal of Physiology and Pharmacology
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• 제1권1호
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• pp.19-25
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• 1997

The C-terminus ends of the second putative transmembrane domains of both $M_1$ and $M_2$ muscarinic receptors contain a triplet of amino acid residues consisting of leucine (L), tyrosine (Y) and threonine (T). This triplet is repeated as LYT-TYL in $M_1$ receptors at the interface between the second transmembrane domain and the first extracellular loop. Interestingly, however, it is repeated in a transposedfashion (LYT-LYT) in the sequence of $M_2$ receptors. In our previous work, we investigated the possible significance of this unique sequence diversity for determining the distinct differential receptor function at the two receptor subtypes. However, we found mutation of the LYTTYL sequence of $M_1$ receptors to the corresponding $M_2$ receptor LYTLYT sequence demonstrated markedly enhanced the stimulation of phosphoinositide (PI) hydrolysis by carbachol without a change in its coupling to increased cyclic AMP formation. In this work, thus, the enhanced stimulation of PI hydrolysis in the LYTLYT $M_1$ receptor mutant was further investigated. The stimulation of PI hydrolysis by carbachol was enhanced in the mutant $M_1$ receptor, and this change was not due to alterations in the rate of receptor desensitization or sequestration. The observed larger response to carbachol at mutant $M_1$ receptors was also not due to an artifact resulting from selection of CHO cells which express higher levels of G-proteins or phospholipase C. Our data suggest that although the LYTTYL sequence in $M_1$ muscarinic receptors is not involved in determining receptor pharmacology, mutation of the sequence enhanced the coupling of $M_1$ receptors to the stimulation of phospholipase C.

• Asian-Australasian Journal of Animal Sciences
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• 제33권9호
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• pp.1378-1386
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• 2020

Objective: Chinese indigenous sheep breeds can be classified into the following three categories by their tail morphology: fat-tailed, fat-rumped and thin-tailed sheep. The typical sheep breeds corresponding to fat-tailed, fat-rumped, and thin-tailed sheep are large-tailed Han, Altay, and Tibetan sheep, respectively. Detection of copy number variation (CNV) and selection signatures provides information on the genetic mechanisms underlying the phenotypic differences of the different sheep types. Methods: In this study, PennCNV software and F-statistics (F_ST) were implemented to detect CNV and selection signatures, respectively, on the X chromosome in three Chinese indigenous sheep breeds using ovine high-density 600K single nucleotide polymorphism arrays. Results: In large-tailed Han, Altay, and Tibetan sheep, respectively, a total of six, four and 22 CNV regions (CNVRs) with lengths of 1.23, 0.93, and 7.02 Mb were identified on the X chromosome. In addition, 49, 34, and 55 candidate selection regions with respective lengths of 27.49, 16.47, and 25.42 Mb were identified in large-tailed Han, Altay, and Tibetan sheep, respectively. The bioinformatics analysis results indicated several genes in these regions were associated with fat, including dehydrogenase/reductase X-linked, calcium voltage-gated channel subunit alpha1 F, and patatin like phospholipase domain containing 4. In addition, three other genes were identified from this analysis: the family with sequence similarity 58 member A gene was associated with energy metabolism, the serine/arginine-rich protein specific kinase 3 gene was associated with skeletal muscle development, and the interleukin 2 receptor subunit gamma gene was associated with the immune system. Conclusion: The results of this study indicated CNVRs and selection regions on the X chromosome of Chinese indigenous sheep contained several genes associated with various heritable traits.

• 전기전자학회논문지
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• 제13권1호
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• pp.65-70
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• 2009

We propose an ultrashort pulse optical code-division multiple-access (O-CDMA) scheme based on a pseudorandom binary M-sequence spectral phase encoding and decoding of coherent mode-locked laser pulses and perform a numerical simulation to analyze its feasibility. We demonstrate the ability to properly decode any of the multiple (eight) 10 Gbit/s users by the matched code selection of the spectral phase decoder. The peak power signal to noise ratio of properly and improperly decoded $8{\times}10 Gb/s$ signals could be greater than 15 for 127 M-sequence coding.

• Genomics & Informatics
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• 제15권4호
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• pp.162-169
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• 2017

Metal binding proteins or metallo-proteins are important for the stability of the protein and also serve as co-factors in various functions like controlling metabolism, regulating signal transport, and metal homeostasis. In structural genomics, prediction of metal binding proteins help in the selection of suitable growth medium for overexpression's studies and also help in obtaining the functional protein. Computational prediction using machine learning approach has been widely used in various fields of bioinformatics based on the fact all the information contains in amino acid sequence. In this study, random forest machine learning prediction systems were deployed with simplified amino acid for prediction of individual major metal ion binding sites like copper, calcium, cobalt, iron, magnesium, manganese, nickel, and zinc.

• Journal of Life Science
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• 제12권1호
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• pp.14-18
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• 2002

Subpopulations of nucleotides that bind specifically to a variety of proteins have been isolated from a population of random sequence RNA/DNA molecules. Roughly one in $10^{13}$ random sequence RNA/DNA molecules folds in such a way as to create a specific binding site for small ligands. Since the development of in vitro selection procedure, more than 50 nucleic acid ligands (aptamers) have been isolated. These molecules are very useful for the study of molecular recognition between nucleic acid and protein/organic compound. In addition to these basic studies this method gives us a dream to produce new drugs against several diseases. We focused on several aptamers which specifically binds to trypsin-like serine proteases (thrombin, human neutrophil elastase, activated protein C and NS3 protease of human hepatitis C virus) and want to introduce their structural characteristics and some functions.

• BMB Reports
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• 제38권6호
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• pp.690-694
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• 2005

Transcription termination of the human mitochondrial genome requires specific binding to termination factor mTERF. In this study, mTERF was produced in E. coli and purified by two-step chromatography. mTERF-binding DNA sequences were isolated from a pool of randomized sequences by the repeated selection of bound sequences by gel-mobility shift assay and polymerase chain reaction. Sequencing and comparison of the 23 isolated clones revealed a 16-bp consensus sequence of 5'-GTG$\b{TGGC}$AGANCCNGG-3' in the light-strand (underlined residues were absolutely conserved), which nicely matched the genomic 13-bp terminator sequence 5'-$\b{TGGC}$AGAGCCCGG-3'. Moreover, mTERF binding assays of heteroduplex and single-stranded DNAs showed mTERF recognized the light strand in preference to the heavy strand. The preferential binding of mTERF with the light-strand may explain its distinct orientation-dependent termination activity.

• 대한의용생체공학회:의공학회지
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• 제28권1호
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• pp.24-28
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• 2007

Presuming that firing neurons have motions inside the MRI magnet due to the interaction between the neuronal magnetic field and the main magnetic field, we applied motion encoding gradients to dissected snail ganglia to observe faster responding MRI signal than the BOLD signal. To activate the snail ganglia in synchronization with the MRI pulse sequence, we used electrical stimulation with the frequency of 30 Hz and the pulse width of 2s. To observe the fast responding signal, we used the volume selected MRI sequence. The magnetic resonance signal intensity, measured with 8 ms long motion encoding gradient with a 20mT/m gradient strength, decreased about $3.46{\pm}1.48%$ when the ganglia were activated by the electrical stimulation.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2005년도 ICCAS
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• pp.1098-1100
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• 2005

The floorplanning problem is an essential design step in VLSI layout design and it is how to place rectangular modules as density as possible. And then, as the DSM advances, the VLSI chip becomes more congested even though more metal layers are used for routing. Usually, a VLSI chip includes several buses. As design increases in complexity, bus routing becomes a heavy task. To ease bus routing and avoid unnecessary iterations in physical design, we need to consider bus planning in early floorplanning stage. In this paper, we propose a floorplanning method for topological constraint consisting of bus constraint and memory constraint. The proposed algorithms based on Genetic Algorithm(GA) is adopted a sequence pair. For selection control, new objective functions are introduced for topological constraint. Studies on floor planning and cell placement have been reported as being applications of GA to the LSI layout problem. However, no studies have ever seen the effect of applying GA in consideration of topological constraint. Experimental results show improvement of bus and memory constraint.