• Journal of the Korean Society of Safety
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• v.36 no.3
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• pp.7-14
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• 2021

Hybrid laminated Al/carbon-fiber-reinforced plastic (CFRP) composites are attracting considerable attention from industries such as aerospace and automobiles owing to their excellent specific strength and specific rigidity. However, when this material is used to fabricate high-pressure fuel storage containers subjected to repeated fatigue loads, fatigue life evaluation for the working load is regulated as an important criterion for operational safety and ease of maintenance. Among the existing evaluation methods for these vessels, the burst test and the hydraulic repeat test require expensive facilities. Thus, the present study aims to develop an improved fatigue life test for Al/CFRP laminated hybrid composites. The test specimen was manufactured using a curved mold considering the shape of a type III high-pressure storage container. The strain-life method was used for fatigue life evaluation, and the life was predicted based on the transition life. The results indicate that the more complex the CFRP stacking sequence, the longer is the transition life. This test method is expected to be useful for ensuring the fatigue safety and economy of hybrid laminate composites.

• Journal of Korea Society of Industrial Information Systems
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• v.15 no.5
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• pp.149-158
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• 2010

A messenger program in this paper was designed and implemented using Use Case Diagram, Class Diagram, Activity Diagram, and Sequence Diagram. We selected total 7 major functionalities such as PC server, login, friends management, chatting, file transfer, account management, etc. The followings are various implementation results from this paper. First, developers could easily understand the desired product and programming flow from using UML Diagrams. Second, PDA users could communicate with developers for a long time without time constraints. Third, there was high usability of UML Diagram in the phase of objected-oriented analysis. Forth, it was easy to update and maintain a program.

• International Journal of Computer Science & Network Security
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• v.22 no.2
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• pp.348-356
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• 2022

The article is devoted to substantiation of theoretical and methodical bases of formation of the organizational-economic mechanism of maintenance of stability of development of region on the bases of influence of potential-forming space in the conditions of formation of creative economy. It was found that the organizational-economic mechanism due to its multifaceted nature does not have a single generally accepted definition, and its acceptable scale and complexity is reflected in the structure, which is very dependent on the scope and conditions of its application, can be very different and contain different elements. In view of this, in order to highlight the characteristics that are inherent in the organizational-economic mechanism of sustainable development of the region on the basis of potential-forming space in the formation of creative economy, the article examines the properties and characteristics of direct organizational and economic mechanism. The necessity of basing the process of formation of any organizational-economic mechanism, including the organizational-economic mechanism of ensuring the sustainability of the region on the basis of the potential-forming space in the conditions of creative economy on the system of principles. In this context, the author's vision is proposed and a set of principles for the formation of organizational-economic mechanism for sustainable development of the region on the basis of the potential of the potential-forming space in the creative economy, as well as revealed the essence of each. According to the structural aspect, the organizational-economic mechanism of ensuring the sustainability of the region's development on the basis of the potential-forming space is proposed to be presented as a set of seven stages, which are implemented in a certain sequence. Within the limits of this research the sequence of realization of the stages making process of formation of the organizational-economic mechanism of maintenance of stability of development of region on the basis of influence of potential-forming space in the conditions of formation of creative economy is defined and their maintenance and essence is presented.

• Environmental Mutagens and Carcinogens
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• v.22 no.3
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• pp.137-141
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• 2002

The SNF2/SW12 family comprises proteins from a variety of species with in vivo functions, such as transcriptional regulation, maintenance of chromosome stability during mitosis, and various types of DNA repair. This study was shown the characterization of hrp2+ gene which was isolated by PCR amplification using the conserved domain of SNF2 motifs. Sequence analysis of hrp2+ gene showed striking evolutionary conservation among the SNF2 family of proteins. The transcript of hrp2+ gene was found to be a 4.7 kb as identified by Northern hybridization. In addition, to determine the transcription initiation site of hrp2+ gene, primer extension analysis was performed. This result showed the band of 64 bp. The transcriptional start point was mapped to a position of 47 base pair from the first ATG codon of translational initiation codon. In order to investigate the inducibility of hrp2+ gene, transcript levels were examined after treating the cells to various DNA damaging agents. The transcripts of hrp2+ were induced by UV-irradiation. But the transcripts were not induced by treatment of 0.25% Methylmethane sulfonate (MMS). These results implied that the effects of damaging agents are complex and different regulatory pathways exist for the induction of this gene.

• Journal of Life Science
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• v.15 no.2 s.69
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• pp.192-196
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• 2005

The SNF2/SW12 family comprises proteins from a variety of species with in vivo functions, such as transcriptional regulation, maintenance of chromosome stability during mitosis, and various types of DNA repair. This study was shown the characterization of hrp2+ gene which was isolated by PCR amplification using the conserved domain of SNF2 motifs. Sequence analysis of hrp2+ gene showed striking evolutionary conservation among the SNF2 family of proteins. The transcript of hrp2+ gene was found to be a 4.7 kb as identified by Northern hybridization. To investigate the inducibility of hrp2+ gene, transcript levels were examined after treating the cells to various DNA damaging agents. The transcripts of hrp2+ were induced by UV-irradiation. But the transcripts were not induced by treatment of $ 0.25\%$ Methylmethane sulfonate (MMS). These results implied that the effects of damaging agents are complex and different regulatory pathways exist for the induction of this gene. Hrp2 protein was purified near homogeneity by combination of affinity chromatography. We tested the purified Hrp2 protein for the helicase activity in an oligonucleotide release assay. However we were unable to detect any helicase activity associated with the Hrp2 protein, indicating that the helicase motifs in Hrp2 are merely indicators of a broader DNA-dependent ATPase activity.

• Journal of Navigation and Port Research
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• v.34 no.5
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• pp.383-388
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• 2010

Lab scale experiment study was carried out for biological process development on cruise. SBR(Sequence Batch Reactor), MBR(Membrane Bioreactor), and MSBR(Membrane Sequence Batch Reactor) system were investigated for practical application on shipboard sewage treatment. From the results it was suggested that MSBR system might be suitable process for cruise in terms of pollutant removal efficiency, maintenance and special environmental conditions of cruise. About 99% of BOD, 98% of COD and 99% of SS were removed in MSBR system. In addition, about 76% of total nitrogen was reduced and the total phosphorus reduction averaged 59%.

• BMB Reports
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• v.40 no.3
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• pp.315-324
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• 2007

The novel $\alpha$-amylase purified from locally isolated strain, Bacillus sp. KR-8104, (KRA) (Enzyme Microb Technol; 2005; 36: 666-671) is active in a wide range of pH. The enzyme maximum activity is at pH 4.0 and it retains 90% of activity at pH 3.5. The irreversible thermoinactivation patterns of KRA and the enzyme activity are not changed in the presence and absence of $Ca^{2+}$ and EDTA. Therefore, KRA acts as a $Ca^{2+}$-independent enzyme. Based on circular dichroism (CD) data from thermal unfolding of the enzyme recorded at 222 nm, addition of $Ca^{2+}$ and EDTA similar to its irreversible thermoinactivation, does not influence the thermal denaturation of the enzyme and its Tm. The amino acid sequence of KRA was obtained from the nucleotide sequencing of PCR products of encoding gene. The deduced amino acid sequence of the enzyme revealed a very high sequence homology to Bacillus amyloliquefaciens (BAA) (85% identity, 90% similarity) and Bacillus licheniformis $\alpha$-amylases (BLA) (81% identity, 88% similarity). To elucidate and understand these characteristics of the $\alpha$-amylase, a model of 3D structure of KRA was constructed using the crystal structure of the mutant of BLA as the platform and refined with a molecular dynamics (MD) simulation program. Interestingly enough, there is only one amino acid substitution for KRA in comparison with BLA and BAA in the region involved in the calcium-binding sites. On the other hand, there are many amino acid differences between BLA and KRA at the interface of A and B domains and around the metal triad and active site area. These alterations could have a role in stabilizing the native structure of the loop in the active site cleft and maintenance and stabilization of the putative metal triad-binding site. The amino acid differences at the active site cleft and around the catalytic residues might affect their pKa values and consequently shift its pH profile. In addition, the intrinsic fluorescence intensity of the enzyme at 350 nm does not show considerable change at pH 3.5-7.0.

• Transactions of The Korea Fluid Power Systems Society
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• v.8 no.4
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• pp.17-23
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• 2011

Proportional control solenoid is a type of modulating valve that can continuously control the valve position with magnetic force of solenoid. Recent microcontroller based digital servocontroller for proportional valve is being developed toward the smart valve with additional features such as enhanced control algorithm for finer process and intelligent on-board diagnosis for maintenance. In this paper, development of servocontroller network control with CAN bus which is free from problems of security and network traffic jam is presented. Design of network control system includes modes of communication between master and slave, assignment of 29bit message identifier and message objects, transaction of communication sequence, etc. Monitoring function and control experiments for remote valve through CAN network prove the extended function of smart valve control system.

• Proceedings of the KIEE Conference
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• 2011.07a
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• pp.1191-1192
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• 2011

We released new triple redundant digital excitation system with real time generator-turbine simulator. One of its great merits is the real time generator-turbine simulator when it was compared with the other products. If excitation system is tripped by unexpected faults, Maintenance man can do easily performance test of digital excitation control board, sequence relay and thyristor switching device of phase controlled rectifier without manufacturer's support. For the verification of this system, It was tested with an actual excitation system implemented on 5kVA M-G Set. After finishing the tests, the trial product will be installed and operated at a 500MW thermal power plant.

• International Journal of Highway Engineering
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• v.16 no.5
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• pp.67-72
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• 2014

PURPOSES : This study focuses on conducting pavement rehabilitation for more deteriorated roads given a limited government budget. METHODS : Therefore, we apply a optimization programming model of the permutation algorithm used for rehabilitating more deteriorated pavement areas, which is subjected to the limited budget. RESULTS : The permutation algorithm was evaluated in terms of determining the maximized rehabilitation of deteriorated pavement areas, using the newly developed performance models such as fatigue cracking, rutting, international roughness index(IRI) through the surveying data from 2006 to 2012. When compared with a traditional model of the so-called worst-first sequence(WFS) method, the permutation algorithm worked better than the WFS method, resulting in covering more deteriorated pavement areas given the limited government budget. CONCLUSIONS : Through a case study, it could be concluded that the permutation algorithm provides more reliable results in terms of rehabilitating more deteriorated pavement areas given the limited budget.