• 제목/요약/키워드: Sequence Analyses

검색결과 939건 처리시간 0.035초

디지탈 육상이동통신을 위한 TCM에 관한 연구 (A Study on TCM for Digital Land Mobile Communication)

  • 방성일;진연강
    • 전자공학회논문지A
    • /
    • 제31A권2호
    • /
    • pp.1-8
    • /
    • 1994
  • In this paper, a model of trellis-coded M-ary PSK (TCM/M-PSK) for digital land mobile communication is realized. Bit error rate(BER) of TCM/M-PSK is calculated theoretically in time selective fading environment due to Doppler spread in the presence of AWGN. cochannel interference. These analyses are employed the receiver with post detection diversity and the direct sequence to improve the BER performance of TCM-MPSK system. It is shown that the CNR required by the diversity reception and the direct sequece(process gain=20dB) TCM-4PSK system is reduced to about 25 dB.

  • PDF

A Flow Analysis Framework for Traffic Video

  • Bai, Lu-Shuang;Xia, Ying;Lee, Sang-Chul
    • 한국공간정보시스템학회 논문지
    • /
    • 제11권2호
    • /
    • pp.45-53
    • /
    • 2009
  • The fast progress on multimedia data acquisition technologies has enabled collecting vast amount of videos in real time. Although the amount of information gathered from these videos could be high in terms of quantity and quality, the use of the collected data is very limited typically by human-centric monitoring systems. In this paper, we propose a framework for analyzing long traffic video using series of content-based analyses tools. Our framework suggests a method to integrate theses analyses tools to extract highly informative features specific to a traffic video analysis. Our analytical framework provides (1) re-sampling tools for efficient and precise analysis, (2) foreground extraction methods for unbiased traffic flow analysis, (3) frame property analyses tools using variety of frame characteristics including brightness, entropy, Harris corners, and variance of traffic flow, and (4) a visualization tool that summarizes the entire video sequence and automatically highlight a collection of frames based on some metrics defined by semi-automated or fully automated techniques. Based on the proposed framework, we developed an automated traffic flow analysis system, and in our experiments, we show results from two example traffic videos taken from different monitoring angles.

  • PDF

잣나무(Pinus koraiensis)의 cDNA library 제작 및 EST 분석 (Construction of a full-length cDNA library from Pinus koraiensis and analysis of EST dataset)

  • 김준기;임수빈;최선희;이종석;노승문;임용표
    • 농업과학연구
    • /
    • 제38권1호
    • /
    • pp.11-16
    • /
    • 2011
  • In this study, we report the generation and analysis of a total of 1,211 expressed sequence tags (ESTs) from Pinus koraiensis. A cDNA library was generated from the young leaf tissue and a total of 1,211 cDNA were partially sequenced. EST and unigene sequence quality were determined by computational filtering, manual review, and BLAST analyses. In all, 857 ESTs were acquired after the removal of the vector sequence and filtering over a minimum length 50 nucleotides. A total of 411 unigene, consisting of 89 contigs and 322 singletons, was identified after assembling. Also, we identified 77 new microsatellite-containing sequences from the unigenes and classified the structure according to their repeat unit. According to homology search with BLASTX against the NCBI database, 63.1% of ESTs were homologous with known function and 22.2% of ESTs were matched with putative or unknown function. The remaining 14.6% of ESTs showed no significant similarity to any protein sequences found in the public database. Gene ontology (GO) classification showed that the most abundant GO terms were transport, nucleotide binding, plastid, in terms biological process, molecular function and cellular component, respectively. The sequence data will be used to characterize potential roles of new genes in Pinus and provided for the useful tools as a genetic resource.

Molecular Cloning and Expression of a Novel Cuticle Protein Gene from the Chinese Oak Silkmoth, Antheraea pernyi

  • Kim Bo Yeon;Park Nam Sook;Jin Byung Rae;Kang Pil Don;Lee Bong Hee;Seong Su Il;Hwang Jae Sam;Chang Jong Su;Lee Sang Mong
    • International Journal of Industrial Entomology and Biomaterials
    • /
    • 제10권1호
    • /
    • pp.11-17
    • /
    • 2005
  • In our research to identify gene involved in the cuticle protein, we cloned a novel cuticle protein gene, ApCP15.5, from the Chinese oak silkmoth, Antheraea pernyi, larvae cDNA library. The gene encodes a 149 amino acid polypeptide with a predicted molecular mass of 15.5 kDa and a pI of 9.54. The ApCP15.5 contained a type-specific consensus sequence identifiable in other insect cuticle proteins and the deduced amino acid sequence of the ApCP15.5 cDNA is most homologous to Tenebrio molitor-C1B ($43\%$ protein sequence identity), followed by Locusta migratoria-76 ($42\%$ protein sequence identity). Northern blot and Western blot analyses revealed that the ApCP15.5 showed the epidermis-specific expression. The expression profile of ApCP15.5 indicated that the ApCP15.5 mRNA expression was detected in the early stages after larval ecdysis and larval-pupal metamorphosis, and its expression level was most significant on the first day of larval ecdysis and pupal stage. The ApCP15.5 was expressed as a 15.5 kDa polypeptide in baculovirus-infected insect cells.

Assessment of the Cerebrospinal Fluid Effect on the Chemical Exchange Saturation Transfer Map Obtained from the Full Z-Spectrum in the Elderly Human Brain

  • Park, Soonchan;Jang, Joon;Oh, Jang-Hoon;Ryu, Chang-Woo;Jahng, Geon-Ho
    • 한국의학물리학회지:의학물리
    • /
    • 제30권4호
    • /
    • pp.139-149
    • /
    • 2019
  • Purpose: With neurodegeneration, the signal intensity of the cerebrospinal fluid (CSF) in the brain increases. The objective of this study was to evaluate chemical exchange saturation transfer (CEST) signals with and without the contribution of CSF signals in elderly human brains using two different 3T magnetic resonance imaging (MRI) sequences Methods: Full CEST signals were acquired in ten subjects (Group I) with a three-dimensional (3D)-segmented gradient-echo echo-planar imaging (EPI) sequence and in ten other subjects (Group II) with a 3D gradient and spin-echo (GRASE) sequence using two different 3T MRI systems. The segmented tissue compartments of gray and white matter were used to mask the CSF signals in the full CEST images. Two sets of magnetization transfer ratio asymmetry (MTRasym) maps were obtained for each offset frequency in each subject with and without masking the CSF signals (masked and unmasked conditions, respectively) and later compared using paired t-tests. Results: The region-of-interest (ROI)-based analyses showed that the MTRasym values for both the 3D-segmented gradient-echo EPI and 3D GRASE sequences were altered under the masked condition compared with the unmasked condition at several ROIs and offset frequencies. Conclusions: Depending on the imaging sequence, the MTRasym values can be overestimated for some areas of the elderly human brain when CSF signals are unmasked. Therefore, it is necessary to develop a method to minimize this overestimation in the case of elderly patients.

Cloning and Characterization of a Rice cDNA Encoding Glutamate Decarboxylase

  • Oh, Suk-Heung;Choi, Won-Gyu;Lee, In-Tae;Yun, Song-Joong
    • BMB Reports
    • /
    • 제38권5호
    • /
    • pp.595-601
    • /
    • 2005
  • In this study, we have isolated a rice (Oryza sativa L.) glutamate decarboxylase (RicGAD) clone from a root cDNA library, using a partial Arabidopsis thaliana GAD gene as a probe. The rice root cDNA library was constructed with mRNA, which had been derived from the roots of rice seedlings subjected to phosphorus deprivation. Nucleotide sequence analysis indicated that the RicGAD clone was 1,712 bp long, and harbors a complete open reading frame of 505 amino acids. The 505 amino acid sequence deduced from this RicGAD clone exhibited 67.7% and 61.9% identity with OsGAD1 (AB056060) and OsGAD2 (AB056061) in the database, respectively. The 505 amino acid sequence also exhibited 62.9, 64.1, and 64.2% identity to Arabidopsis GAD (U9937), Nicotiana tabacum GAD (AF020425), and Petunia hybrida GAD (L16797), respectively. The RicGAD was found to possess a highly conserved tryptophan residue, but lacks the lysine cluster at the C-proximal position, as well as other stretches of positively charged residues. The GAD sequence was expressed heterologously using the high copy number plasmid, pVUCH. Our activation analysis revealed that the maximal activation of the RicGAD occurred in the presence of both $Ca^{2+}$ and calmodulin. The GAD-encoded 56~58 kDa protein was identified via Western blot analysis, using an anti-GAD monoclonal antibody. The results of our RT-PCR analyses revealed that RicGAD is expressed predominantly in rice roots obtained from rice seedlings grown under phosphorus deprivation conditions, and in non-germinated brown rice, which is known to have a limited phosphorus bioavailability. These results indicate that RicGAD is a $Ca^{2+}$/calmodulin-dependent enzyme, and that RicGAD is expressed primarily under phosphate deprivation conditions.

DNA marker 지문법에 의한 취나물 5종 (청옥취 , 개미취 , 참취 , 수리취 , 곰취)의 비교연구 (Comparative studies of the five edible mountain vegetables by DNA marker fingerprinting)

  • 유기억
    • 한국자원식물학회지
    • /
    • 제9권3호
    • /
    • pp.305-310
    • /
    • 1996
  • 유용 취나물 5종류의 종간 유사도 및 종내 변이를 파악하기 위하여 38개체를 대상으로 PCR 방법을 이용하여 비교분석하였다. 사용된 62가지 primer 중에서 38개체 전체에서 band를 보여준 것은 10가지였고, 이를 통해 얻어진 총 band의 수는 176개로 나타났다. 종내변이는 청옥취가 31.1%에 해당하는 15개의 polymorphic band를 가져 변이가 가장 낮았으며 곰취는 61.1%로 가장 높게 나타났다. 군집분석 결과 조사된 38개체는 유사도 0.93에서 5종의 구분이 가능하였으며 38개체 각각은 유사도 0.62-0.99의 유사성을 갖는 것으로 나타났고 종내변이는 0.93이상으로 높게 나타나 변이가 적은 것으로 나타났다. 이상의 결과에서 PCR방법을 이용한 결과가 취나물 5종류를 구분하는데 유용하게 사용되었으며 군집분석 결과 종내 변이가 매우 낮은 것으로 나타났다.

  • PDF

Expressed Sequence Tag Analysis of the Erythrocytic Stage of Plasmodium berghei

  • Seok, Ji-Woong;Lee, Yong-Seok;Moon, Eun-Kyung;Lee, Jung-Yub;Jha, Bijay Kumar;Kong, Hyun-Hee;Chung, Dong-Il;Hong, Yeon-Chul
    • Parasites, Hosts and Diseases
    • /
    • 제49권3호
    • /
    • pp.221-228
    • /
    • 2011
  • Rodent malaria parasites, such as Plasmodium berghei, are practical and useful model organisms for human malaria research because of their analogies to the human malaria in terms of structure, physiology, and life cycle. Exploiting the available genetic sequence information, we constructed a cDNA library from the erythrocytic stages of P. berghei and analyzed the expressed sequence tag (EST). A total of 10,040 ESTs were generated and assembled into 2,462 clusters. These EST clusters were compared against public protein databases and 48 putative new transcripts, most of which were hypothetical proteins with unknown function, were identified. Genes encoding ribosomal or membrane proteins and purine nucleotide phosphorylases were highly abundant clusters in P. berghei. Protein domain analyses and the Gene Ontology functional categorization revealed translation/protein folding, metabolism, protein degradation, and multiple family of variant antigens to be mainly prevalent. The presently-collected ESTs and its bioinformatic analysis will be useful resources to identify for drug target and vaccine candidates and validate gene predictions of P. berghei.