• Title/Summary/Keyword: Septicemia

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Study on the Hemolysin from Marine V. vulnificus (해양 V. vulnificus의 Hemolysin에 관한 연구)

  • 이봉헌;박흥재
    • Journal of Environmental Science International
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    • v.6 no.3
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    • pp.225-229
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    • 1997
  • A halophilic V. vulnificus is an estuarine microorganism that has been associated with fatal wound Infection and life-threatening septicemia. Hemolysin is defined as toxic substance produced by various species of bacteria Including V. vulnificus. Hemolysin from marine V. vulnificus was purified and the effect of pH, temperature. metal ion on the activity of hemolysin, and thermostability of hemolysin were tested in this study. Hemolysin iysed the sheep red blood cell and the optimum pH was 8.0, the optimum temperature was 4$0^{\circ}C$, and $K^+$ increased but $Mn^{2+}$ decreased the hemolyic activity of hemolysin, but hemolysin was unstable to heat.

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A Study of Hospital Infection in the Postoperative Patients (수술후 병원 감염 발생과 입원일수 및 수술 소요 시간과의 관계)

  • 박정호;윤혜상
    • Journal of Korean Academy of Nursing
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    • v.16 no.2
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    • pp.70-76
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    • 1986
  • This study was aimed to observe the incidences and types of hospital infections and to compare the length of hospitalization and of the operation between the infected Patients and those of non-infected after the operations. The subject of study were 465 patients who had been operated surgically in a University Hospital from March 1 to April 30, 1985. The data were collected by reviewing medical charts of subjects. The criteria to diagnose hospital infection for this study had been revised the one utilized at University of Virginia Hospital in the U.S. Summary of the results were as follows: 1. The incidence rate of hospital infection was 6 %. The type of infection with higher incidence rate in order were wound infection(28.5%)urinary infection(28.5%), fever of unknown origin (25%) and septicemia (18%). 2. There was a statistically significant difference in the duration of hospitalization between the patient group without it (t=265.2, p<0.005). 3. There was a statistically significant difference in the duration of operation between the patient group with it (t=75.0, p<0.005).

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Discovery of Performance Traits-Linked Microsatellite Markers in Channel Catfish (Ictalurus punctatus)

  • Kim, Soon-Hag
    • Journal of Aquaculture
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    • v.18 no.2
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    • pp.130-132
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    • 2005
  • Genomics research has two ultimate applied goals: to Isolate and clone genes of economic importance for bio-technology and gene-assisted selection (GAS), and to locate and use markers for marker-assisted selection (MAS) in selective breeding programs. To this end, we have identified linked markers for feed conversion efficiency growth rate, and disease resistance to enteric septicemia of catfish (ESC). Three microsatellite markers Ip266, Ip384, and Ip607 were identified to be linked to feed conversion efficiency. Similarly one marker each was identified to be linked to growth rate (Ip607) and disease resistance to ESC (Ip477). Ip607 marker linked to both growth rate and feed conversion efficiency, indicating that the QTL for both growth rate and feed conversion efficiency may either be the same or located in the same chromosomal region in the catfish genome. On phenotypic evaluation, certain traits such as growth rate can be accurately evaluated by body weight evaluation while other traits such as disease resistance can be quite complex. The linked DNA markers will be highly useful for MAS programs and for directing further efforts of genomic mapping for important quantitative traits.

A Case of Lemierre Syndrome (Lemierre 증후군 1예)

  • Kim, Ye-Won;Kim, Dong-Hyun;Kim, Do-Hyun;Jeon, Eun-Ju
    • Korean Journal of Bronchoesophagology
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    • v.16 no.2
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    • pp.149-153
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    • 2010
  • Lemierre syndrome, also known as postanginal sepsis, is a severe complication of an acute oropharyngeal infection that result in septic thrombophlebitis of the internal jugular vein with subsequent septicemia. This disease is relatively rare, but it has significant morbidity and is potentially fatal. Early diagnosis based on computed tomography with contrast enhancement is mandatory and immediate treatment including intravenous antibiotics, anticoagulants, or surgical approach should be considered. We report a case of Lemierre syndrome after deep neck infection which was successfully treated using antibiotics and anticoagulants.

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Effect of Use of SPS Agar and SGS Broth and Storage of Specimen on the Isolation of Vibrio vulnificus (Vibrio vulnificus 분리율에 대한 SPS Agar와 SGP Broth의 사용 및 검체 저장의 영향)

  • Chong, Yun-Sop;Lee, Samuel Y.;Kim, Shin-Moo
    • The Journal of the Korean Society for Microbiology
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    • v.22 no.2
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    • pp.103-108
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    • 1987
  • Vibrio vulnificus septicemia is nor-rare diease in Korea. Carriage rate of the orgaism by shellfish is not well known. In this study performance of SPS agar and SGP broth and effect of storage of specimen in the isolaton was determined using the shellfish specimens collected from the coast and market of Koonsan city. Isolation rate was similar with TCBS and with SPS, but the rate became much highher after enrichment in SGP broth. 80% of oyster speimes were positve when inoculated immediately, but the rate dropped rapidly after storage of specimens at freezing temperature for sometime. All of the isolates fermented lactose in 2 days. A few isolates were not identfiable with API 20E system, because of acid prduction from melibiose. Serover 04 was the frequent isolates.

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Generation of heterologous proteins-expressing recombinant snakehead rhabdoviruses (rSHRVs) using reverse genetics

  • Kwak, Jun Soung;Ryu, Sujeong;Kim, Ki Hong
    • Journal of fish pathology
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    • v.33 no.2
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    • pp.163-169
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    • 2020
  • Snakehead rhabdovirus (SHRV) is different from other fish novirhabdoviruses such as viral hemorrhagic septicemia virus (VHSV), infectious hematopoietic necrosis virus (IHNV), and hirame rhabdovirus (HIRRV) in that it replicates at high temperatures. Therefore, the delivery of foreign proteins to fish living at high water temperature would be possible by using recombinant SHRVs. In the present study, to evaluate the possible use of SHRV as a vehicle for foreign proteins delivery, we generated a recombinant SHRV that contains an enhanced-GFP (eGFP) gene between nucleoprotein (N) and phosphoprotein (P) genes (rSHRV-A-eGFP), and another recombinant SHRV expressing two heterologous genes by inserting an eGFP gene between N and P genes, and mCherry gene between P and M genes (rSHRV-AeGFP-BmCherry). Epithelioma papulosum cyprini (EPC) cells infected with the recombinant SHRVs showed strong fluorescence(s), suggesting the possible availability of recombinant SHRVs for the development of combined vaccines by expressing multiple foreign antigens.

Isolation of atypical Aeromonas salmonicida in cultured starry flounder (Platichthys stellatus) (양식산 강도다리(Platichthys stellatus)에서의 비정형 Aeromonas salmonicida 분리)

  • Wi-Sik, Kim;Min-Su, Kwon;Hwi-Jin, Kim;Myung-Joo, Oh;Kyoung-Hui, Kong
    • Journal of fish pathology
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    • v.35 no.2
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    • pp.247-250
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    • 2022
  • About 6.7% mortality was reported in a starry flounder (Platichthys stellatus) aquaculture farm in 2022. Most of the diseased fish showed a loss of pectoral fin, hemorrhages on muscle and gills, pale gills, enlarged spleen, and nodules on kidney. Parasites, fungi or viruses (viral hemorrhagic septicemia virus and hirame novirhabdovirus) were not detected from diseased fish. However, numerous bacteria were isolated from liver, spleen and kidney. Nucleotide sequences of the A-protein-encoding virulence array protein gene (vapA) of the bacteria showed 99.93% identity with Aeromonas salmonicida subsp. masoucida. This study is the first report of isolation of atypical A. salmonicida in cultured starry flounder in Korea.

Streptococcus suis causes bacterial meningitis with hearing loss in patients without direct exposure to pigs in a regional pork industry territory

  • Joong-Goo Kim;Gil Myeong Seong;Young Ree Kim;Sang Taek Heo;Jeong Rae Yoo
    • Journal of Medicine and Life Science
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    • v.20 no.1
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    • pp.43-47
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    • 2023
  • Streptococcus suis (S. suis) is an emerging zoonotic pathogen that causes bacterial meningitis in humans. S. suis is an encapsulated gram-positive facultative anaerobic bacterium and is an important pathogen in pigs. This infectious disease usually manifests in humans as meningitis, endocarditis, septicemia, and arthritis. Most cases originate in Southeast Asia, and human S. suis infections are often reported in countries with a high density of pigs. Meningitis is a common clinical manifestation of S. suis infection. Moreover, hearing loss is a common complication that can be bilateral, profound, and/or permanent. This report presents two cases of bacterial meningitis and hearing loss caused by S. suis in patients without a history of direct exposure to pigs in an intensive pork industry region.

Bacterial Sepsis Associated with a Captive State Caused by Edwardsiella tarda in a Eurasian Brown Bear (Ursus arctos arctos)

  • Min-Goo Seo;Kyung-Yeon Eo;Dongmi Kwak;Kyoo-Tae Kim
    • Journal of Veterinary Clinics
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    • v.40 no.1
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    • pp.78-82
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    • 2023
  • Edwardsiella (E) tarda belongs to the Enterobacteriaceae family and is a motile, gram-negative, rod-shaped, facultative anaerobe regarded as an opportunistic and food-borne pathogen in animals and humans. A 21-year-old male Eurasian brown bear (Ursus arctos arctos) died suddenly without any preliminary signs. Necropsy performed according to standard protocol revealed swollen abdomen with hemorrhagic congestions of the gastroenteric organs, ascites, and hemorrhagic exudates around the mouth. The liver showed discoloration, along with a severely swollen and multiple hemorrhages of the spleen, an elongated gallbladder, and a congested cortex and medullar lesion of kidneys. The stomach contained semi-liquid exudates and undigested chicken exuding a decayed odor. The stomach membranes were dark-gray in color with several cysts in the fundus lesions. Rod-shaped bacteria were found in the major organs by Giemsa staining, identified as E. tarda using a biochemical rapid diagnostic identification kit.