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Effects of Pretense Inhibitors and Antioxidants on In Vitro Survival of Porcine Primordial Germ Cells

  • Lee, Chang-Kyu;Jorge A. Piedrahita
    • Proceedings of the Korean Society of Developmental Biology Conference
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    • 2001.10a
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    • pp.47.1-47
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    • 2001
  • One of the problems associated with in vitro culture of primordial gern cells (PGCs) is the large loss of cells during the initial period of culture. This study characterized the initial loss and determined the effectiveness of two classes of apoptosis inhibitors, protease inhibitors and antioxidants, on the ability of the porcine PGCs to survive in culture. Results from electron microscopic analysis and in situ DNA fragmentation assay indicated that porcine PGCs rapidly undergo apoptosis when placed in culture. Additionally, \ulcorner2-macroglobulin, a protease inhibitor and cytokine carrier, and N-acetylcysteine, an antioxidant, increased the survival of PGCs in vitro. While other protease inhibitors tested did not affect survival of PGCs, all antioxidants tested improved survival of PGCs (p<0.05). Further results indicated that the beneficial effect of the antioxidants was critical only during the initial period of culture. Finally, it was determined that in short-term culture, in the absence of feeder layer, antioxidants could partially replace the effect(s) of growth factors and reduce apoptosis. Collectively, these results indicate that the addition of \ulcorner2-macroglobulin and antioxidatns can increase the number of PGCs in vitro by suppressing apoptosis.

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A Study on Development of Interactive Smart Network(IoT)-based Subway Platform Disaster Response System (사물인터넷 기반 지하철 역사공간 재난대응 시스템 개발에 관한 연구)

  • Park, Mi-yun;Koo, Won-yong;Park, Wan-soon;Park, Eunchurn;Moon, Byung-Gyu;Kwon, Se-gon
    • Journal of Korean Society of Disaster and Security
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    • v.9 no.1
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    • pp.19-24
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    • 2016
  • In this study, we conducted a study on the development of Internet of Things-based disaster response system. We researched the method of building the network-based disaster prevention system using a client machine that the sensor network and the keeper. Also, we developed the algorithm for optimal evacuation shelter based on spanning tree algorithm. The system is tested actually in Seoul Gaepo station, and we verified the usability of the system.

Statistical Estimation of the Number of Contending Stations and its Application to a Multi-round Contention Resolution Scheme

  • Jang, Seowoo;Choi, Jin-Ghoo;Yoon, Sung-Guk
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.10 no.9
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    • pp.4259-4271
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    • 2016
  • With the increased popularity of IEEE 802.11 WLAN, the density of the WLAN devices per access point has also increased, resulting in throughput performance degradation. One of the solutions to the problem is improving the protocol efficiency by a using multi-round contention scheme. This paper first discusses how to estimate the number of contending stations in a WLAN network by using minimum elapsed backoff counter values that can be easily monitored by each station. An approximate closed form expression is derived for the number of active contending stations using the smallest backoff counter value in the network. We then apply this result to adapt the number of contending rounds according to the network loading level to enhance the throughput performance of a multi-round contention scheme. Through simulation, we show that the accuracy of the estimation algorithm depends on the contention parameters of W and the number of backoff counter observing samples, and found a reasonable value for each parameter. We clearly show that our adaptive multi-round contention scheme outperforms the standard contention scheme that uses a fixed number of rounds.

Phage Typing and Lysotype Distribution of Xanthomonas axonopodis pv, citri, the Causal Agent of Citrus Bacterial Canker in Korea

  • Myung, Inn-Shik;Yongsup Cho;Lee, Young-Hee;Kwon, Hyuk-Mo
    • The Plant Pathology Journal
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    • v.17 no.6
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    • pp.336-341
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    • 2001
  • The distribution of citrusphages and phage types of Xanthomonas axonopodis pv. citri was investigated in Korea. Forty-eight strains of the bacterial pathogen and 28 bacteriophage strains were isolated from citrus leaves showing the citrus canker symptom. Only a single bacteriophage group, named CPK, was identified based on their aggressiveness to the bacterial pathogen. The bacterial strains were differentiated into two Iysotypes based on their sensitivity to CPK. Lysotype I, which was sensitive to CPK, was more predominant (96%), while only 4% belonged to Iysotype II, which was resistant to CPK. Among the 13 xanthomonads including Iysotype A and Iysotype B of X axonopodis pv. citri, CPKs were only aggressive to BC 83 (=Xc 62) strain of X. axonopodis pv, citri reported as Iysotype A. Thus, bacterial pathogens and citrusphages related to citrus plants mainly distributed in Korea were presumed as Iysotype A of X. axonopodis pv, citri, and Iysotype A-infecting CP$_1$ respectively.

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Adjustment factors of Precipitation using One-minute Data in Seoul (서울지방 1분 자료를 이용한 강수자료의 환산계수 산정)

  • Jo, Han-Seong;Um, Myoung-Jin;Cho, Won-Cheol;Cho, Joo-Young
    • Proceedings of the Korea Water Resources Association Conference
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    • 2006.05a
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    • pp.1506-1510
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    • 2006
  • 현재 기상청에서 제공하는 강수자료는 정시자료로서 수문학적 의미의 임의지속시간 강수자료라 볼 수 없다. 따라서 이러한 정시자료를 임의지속시간 강수자료로 변환하여 사용하여야 한다. 이러한 환산계수는 국외에서는 Weiss(1964), Dwyer와 Reed(1995) 등에 의하여 제시되어졌고, 국내에서는 김규호 등(1988)등이 환산계수를 제시한 바 있다. 그렇지만 기존 연구의 자료들은 목측에 의한 자료로서 많은 불확실성을 가지고 있다. 최근 관측기기의 발달에 의하여 기상청에서는 1분 단위의 관측 자료를 구축하였다. 따라서 본 연구에서는 이러한 1분 단위 강수자료를 이용하여 수문학적 의미의 임의지속시간 강수자료를 적출하여 보다 정확하게 강수자료의 환산계수(Adjustment factor)를 구축하는데 목적이 있다. 본 연구에서는 서울지방 7개 자동기상관측소(AWS:Automatic Weather Station)에서 관측된 6개년(2000년${\sim}$2005년) 1분 강수자료를 이용하여 고정시간 연 최대강수량과 임의시간 연 최대강수량간의 관계를 연구하였다. 1분 강수자료를 이용하여 고정시간과 임의지속시간에 대한 연 최대치 강수 계열을 구축.도시한 후 선형회귀분석에 의해 선정된 계수를 환산계수로 제시하였다. 고정시간 1시간부터 24시간까지의 최대강수량과 임의시간 간격 최대강수량의 비율을 분석한 결과 환산계수는 지속시간이 증가함에 따라 비선형적으로 감소하는 것으로 나타났다. 이러한 관계를 이용하면 정시 강수자료를 보다 정확하게 임의지속시간 강수자료로 환산할 수 있을 것으로 판단된다.

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Antioxidative Activity and Varietal Difference of Cyanidin 3-glucoside and Peonidin 3-glucoside Contents in Pigmented Rice

  • Ryu, Su-Noh;Han, Sang-Jun;Park, Sun-Zik;Kim, Hong-Yeol
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.45 no.4
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    • pp.257-260
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    • 2000
  • The content of anthocyanin, cyanidin 3-glucoside (C3G) and the peonidin 3-glucoside (P3G) from 591 rice cultivars and the antioxidative activities of MeOH extract from 8 rice cultivars were evaluated. Among them, C3G content of pigmented rice were ranged from 0 to 451.9mg in 100 g brown rice, while the P3G contents were in the range from 0 to 42.7mg in 100g brown rice. There was no correlation between C3G and P3G content. Total anthocyanin content ranged from 0 to 475.1mg in 100g brown rice. The antioxidative activity, the scavenging activity on DPPH (2,2-diphenyl-picryl-hydrazyl) radical of MeOH extracts from rice grain, were different according to cultivars. The activity of blockish purple pericarp rice cultivars was twice stronger than that of white pericarp cultivar. Especially, the antioxidative activity of Heugjinjubyeo was four times stronger than that of white pericarp cultivar. The scavenging effect on DPPH radical in rice extracts was related to the total anthocyanin contents of the extracts.

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The Research of Pseudolite technology by comparison with each applications for marine applications (해양분야 응용을 위한 의사위성 실내항법기술의 적용 대상별 비교 연구)

  • Shim, Woo-Seong;Suh, Sang-Hyun;Lee, Sang-Jeong;Park, Chan-Sik;Ki, Chang-Don
    • Proceedings of the Korea Committee for Ocean Resources and Engineering Conference
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    • 2002.05a
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    • pp.6-11
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    • 2002
  • A term of GNSS(Global Navigation Satellite System) is widely used to represent a navigation method for global area using satellite in space orbit 1his system can provide accurate and continuous position, and timing sources synchronized to UTC. There are, however, certain disadvantage that system can not operate without line of sight environment to satellite, or system failure of either satellite or control station. It is the pseduolite technology for using indoor and also for back-up equipment of foreign system failure. Especially, ocean applications widely use the GNSS system for navigation, surveying, timing, and management of traffic, so, system failure of GNSS will be very critical problem to affect many aspects of ocean field. In this paper, we experimented the pseudolite technology for several application field to compare the result in different environment. We used the common CDGPS algorithm for in-door navigation and experimented in ocean engineering basin with metallic wall and gymnasiums with concrete wall. We also investigated the comparison result and considerations for ocean applications of pseudolite technology.

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Effect of Fertilizer and Straw Mulching on Winter Discoloration and Spring Regrowth of Lawn Grasses

  • Lee, Jin-Wook;Lee, Ho-Jin
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.46 no.2
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    • pp.134-138
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    • 2001
  • This experiment evaluates wintering ability to maintain green color of lawn grasses during winter and investigates the effects of top dressing of fertilizer on improving green color during regrowth. Kentucky blue-grass could maintain green color and leaf chlorophyll content better than tall fescue and creeping bentgrass in winter. All three grasses in this experiment have shown the excellent wintering ability. In enhancing the recovery of green color at the early stage of regrowth, the mulching effect with rice straw was highly significant for creeping bentgrass. Green color recovery in grasses during its regrowth was better at the top dressing plots than at the plots without top dressing, but when fertilizer application levels were increased, green color in lawn grass did not significantly change. Although green color in tall fescue, Kentucky bluegrass, and Korean lawngrass could be maintained during summer, the green color of creeping bentgrass is reduced significantly with high temperature. Top dressing after winter and mowing improved leaf chlorophyll content and green color in tail fescue and Kentucky bluegrass significantly. However, Korean lawngrass did not respond significantly with increased levels of fertilizer.

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Continuous Measurements of Reduced Sulfur Gases in Urban Air (연속측정방법을 이용한 도심권 대기질 내 저농도 황화합물의 관측에 대한 연구)

  • Choi Ye-Jin;Kim Ki-Hyun;Oh Sang-In;Shon Zang-Ho
    • Journal of Korean Society for Atmospheric Environment
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    • v.20 no.2
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    • pp.195-204
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    • 2004
  • In this study, the concentrations of major reduced sulfur compounds (H$_2$S, $CH_3$SH, DMS, and DMDS) were determined from ambient air in a monitoring station located in the mid-eastern area of Seoul. Measurements of sulfur species were conducted by the combination of on -line air sampling, thermal desorption, and capillary GC/PFPD analysis. A total number of 143 hourly samples were collected in the two time periods set between June and July 2003. The mean concentrations of four sulfur species measured in the whole study period were found on the order: DMS (535$\pm$183) > H$_2$S (47$\pm$10) > DMDS (35$\pm$22) > $CH_3$SH (6.19$\pm$29.4 pptv). The results of this study show that the concentrations of DMS at the study area are generally higher than those reported previously in the oceanic environments, while those of other sulfur species are not easy to compare with due to the lack of data. The H$_2$S concentrations were generally higher during the daytime than the nighttime, whereas those of others generally exhibited a reversed diurnal pattern. The overall results of our study suggest that the distribution of major reduced S compounds should be controlled by diverse processes in the urban area.

A study on Monitoring for CFC-12 and CFC-11 in the atmosphere near Mt. Kwan-Ak. (관악산 대기 중의 CFC-12 및 CFC-11 모니터링에 관한 연구)

  • 김경렬;민동하;박미경;김은희;최상화;조하만;남재철
    • Journal of Korean Society for Atmospheric Environment
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    • v.10 no.1
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    • pp.41-48
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    • 1994
  • A monitoring system for atmospheric CFC-12 and CFC-11 has been established at Mt. Kwan-Ak, Seoul National University (SNU) since July, 1991. The concentrations showed quite a large variation ranging from 495 to 37600 pptv (pptv=part per trillion, 10$^{-12}$ , v/v) for CFC-12 and from 233 to 12100 pptv for CFC-11 due to many local sources. However, monthly medians show rather limited ranges ; 553~765 pptv for CFC-12 and 301~431 pptv for CFC-11. Furthermore minimum concentrations could be defined relatively well during the whole period of observation. The regional background concentrations in 1993 near SNU wer estimated as 533 pptv for CFC-12 and 293 pptv for CFC-11. These values are very comparable to global averages in Northern Hemisphere, 523 pptv for CFC-12 and 287 pptv for CFC-11, reflecting the fast atmospheric mixing processes within the hemisphere. Examinations with meteorological parameters such as wind speed and direction suggest the possible measurement-window at SNU, appropriate for regional monitoring. Studies for improving the monitoring capability of the SNU station such as automation of the analysis system along with correlation with other meteorological parameters, are in progress at the present time.

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