• 생명과학회지
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• 제9권5호
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• pp.548-555
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• 1999

Long-term effects of Ganoderma lucidum (GL) on memory and oxidative stress of senescence-accelerated mice (SAM) were investigated. Senescence-resistant (R1) and prone (P8) strains of SAM were fed GL diets, premixed with low (20 mg/kg/day, T1) or high (200 mg/kg/day, T2) levels of GL powder for 9 months starting from young (3 months of age) or for 5 months starting from old (7 months of age). After the final feeding at 12 months of age, all animals were subjected to passive avoidance test for the evaluation of memory function. In addition, the changes in hepatic thiobarbituric acid-reactive substance (TBARS) and glutathione were analyzed. SAMP8 fed GL diets from old age (7 months) exhibited the improvement of memory, although GL rather inhibited the memory function of both SAMR1 and SAMP8 mice fed diets from young (3 months of age). Hepatic TBARS contents were decreased in SAMP8 fed high GL diet for 9 months and in SAMR1 fed low GL diet for 5 months. Hepatic glutathione content was also remarkably increased in SAMR1 following both feeding periods, and less extent in SAMP8 fed diet for 5 months of age. Taken together, it is proposed that GL extracts may play an anti-aging role through antioxidant action, and thereby may improve the senescence-related memory dysfunction.

• Journal of Photoscience
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• 제9권2호
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• pp.418-420
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• 2002

We measured previously the lipid hydroperoxides level in the brain and peripheral organs such as heart, liver, lung and kidney of senescence acceIerated-prone (SAMP8) and -resistant(SAMR1) mice at 3,6 and 9 months of age. It was found that the lipid hydroperoxide leve1s in the brain did not show any age-dependent change, and that they Were significantly higher in SAMP8 than in SAMR1 over the defined periods. In contrast, the lipid hydroperoxide leve1s in the peripheral organs, including liver, Were increased with aging in both substrain, and they were significantly higher in SAMP8 than in SAMR1 at 3 and 6 months of age. In addition, the lipid hydroperoxide levels in the peripheral organs were higher than those in the brain in both substrains. To elucidate the difference of lipid hydroperoxide levels between the brain and the peripheral organs, we further carried out lipid component analysis in the brain and liver, one of the peripheral organs, of SAMP8 and SAMR1 at 6 months of age.

• 한국식품영양과학회지
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• 제41권9호
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• pp.1253-1258
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• 2012

본 연구에서는 복분자 섭취가 노화에 의한 기억력 감퇴에 개선 효과를 나타내는지 조사하기 위해 노화촉진마우스 (SAM)를 사용하여 평가하였다. 28주령 SAMP8 마우스에 복분자 시료를 100 mg/kg body weight/day로 8주간 투여한 후 수동회피시험과 수중미로시험을 실시하여 기억력 개선효과를 평가하였다. 수동회피시험에서 노화 촉진 동물(SAMP8)은 대조동물(SAMR1)에 비해 밝은 방에서 체류시간이 현저히 감소하였다. 복분자를 섭취한 노화 촉진 동물의 밝은 방에서의 체류 시간은 복분자를 섭취하지 않은 동물에 비해 현저히 증가하였다. 수중미로실험에서 대조 동물에 비해 노화 촉진 동물의 평균 탈출잠복기는 길었고, 복분자를 섭취한 경우 평균 탈출잠복기가 유의적으로 감소하였다. 뇌의 대뇌피질과 해마 조직에서 대조군에 비해 노화 촉진 동물의 acetylcholinesterase 활성이 증가하였다. 복분자를 섭취한 노화 촉진 동물의 해마 조직 내 acetylcholinesterase는 복분자를 섭취하지 않은 동물에 비해 유의적으로 감소하였다. 이 결과들은 복분자가 노화촉진마우스에서 노화에 의한 기억력 감퇴에 대한 개선 효과를 나타내며, 이는 콜린성 신경전달에 중요한 역할을 하는 acetylcholinesterase 활성 감소를 통해 이루어짐을 나타낸다. 향후 더 많은 연구가 수행 되어야 하나, 본 연구는 복분자가 노인의 기억력 개선제 개발에 좋은 기능성 소재로 활용할 수 있음을 제시한다.

• 생명과학회지
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• 제19권10호
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• pp.1463-1467
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• 2009

Telomeres, comprised of tandem repeats of TTAGGG sequences, are special nucleoprotein structures that protect and stabilize chromosome ends. These structures form the crux of the telomere concept of aging, senescence and genomic instability. The classic terminal restriction fragment (TRF) analysis to quantify the amount of telomeric DNA is disadvantageous in species containing ultra long telomeres like in mice (100Kb). In this study, we used a more sensitive quantitative fluorescence in situ hybridization (Q FISH) technique to quantify telomeric DNA, and used it as a biological aging marker in mice. 12 litters each of Senescence-Resistant (SAMR1) and -Prone (SAMP1) known as senescence accelerated mouse strains were purchased from Central Lab, Animal Inc. We quantified the amount of telomeric DNA using telomere specific DNA probes on the two strains of male mice at 8 weeks, 18 weeks and 26 weeks of age. The amount of telomeric DNA correlated with aging and age associated changes in body and organ weight between SAMR1 and SAMP1 strains of mice. These data suggest the usefulness of the amount of telomeric DNA as a biological aging marker in human aging studies.