• 디지털융복합연구
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• 제19권4호
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• pp.291-303
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• 2021

이 연구는 유튜브(YouTube)에 나타난 필사 문화의 특성을 서사 분석 방법을 통해 알아보고자 하였다. 연구 결과 유튜브의 필사 문화에서 다섯 가지의 의미 있는 특성을 확인할 수 있었다. 유튜브의 필사 문화는 첫째, 효율적인 글쓰기와 학습의 방편이라는 특성을 지니고 있었다. 둘째, 텍스트를 더 깊이 읽고 이해하기 위한 필사라는 특성이 있었다. 셋째, 내 글쓰기로 나아가는 전략이라는 특성을 가지고 있었다. 넷째, 유튜버들은 필사를 통해 자기 치유와 위안의 시간을 갖고 있었다. 다섯째, 유튜브의 필사 문화는 왼손 글쓰기와 디지털 필사로 확장·진화하고 있었다. 유튜브의 필사 주체들은 글쓰기와 학습, 정신 수양의 방편으로 필사를 해왔던 전통적인 필사 문화에서 더 나아가 적극적이고 능동적으로 자신들의 글쓰기 전략을 발전시키고 왼손 글쓰기와 디지털 필사로 필사 문화의 외연을 확장시켜 가고 있었다.

• The Plant Pathology Journal
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• 제15권3호
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• pp.131-136
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• 1999

In fungi known as ascomycetes, ability to mate is controlled by a single mating type (MAT) locus with two dissimilar sequences called idiomorphs carrying genes encoding transcription factors that are unrelated to each other. Fungi requiring strains with different MAT genes to complete the sexual process are heterothallic (self-sterile); species in which as single strain is able to undergo sexual reproduction are homothallic (self-fertile). Previous analysis of sequences from several heterothallic and homothallic species of the ascomycete genus Cochliobolus showed that homothallics evolve from heterothallics and that each known Cochliobolus homothallic species arose independently, from a different heterothallic ancestral species. Here we report detailed comparative analyses of MAT sequences ad their flanking regions, and show that: (1) The level of MAT gene similarity is not correlated with reproductive life style; (2) MAT proteins from all Cochliobolus species are conserved within the transcription factor signature sequences; they are not conserved in the carboxy terminal half of MAT-1, or third of MAT-2, except in those from very closely related species; (3) A gene (ORF1) of unknown function, consistently found on the MAT flank, is more conserved than are the MAT genes themselves; (4) The intergenic sequences diverge sharply among species.

• International Journal of Oral Biology
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• 제46권1호
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• pp.1-6
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• 2021

Since the outbreak of coronavirus disease 2019 (COVID-2019), the infection has spread worldwide due to the highly contagious nature of severe acute syndrome coronavirus (SARS-CoV-2). To manage SARS-CoV-2, the development of diagnostic assays that can quickly and accurately identify the disease in patients is necessary. Currently, nucleic acid-based testing and serology-based testing are two widely used approaches. Of these, nucleic acid-based testing with quantitative reverse transcription-PCR (RT-qPCR) using nasopharyngeal (NP) and/or oropharyngeal (OP) swabs is considered to be the gold standard. Recently, the use of saliva samples has been considered as an alternative method of sample collection. Compared to the NP and OP swab methods, saliva specimens have several advantages. Saliva specimens are easier to collect. Self-collection of saliva specimens can reduce the risk of infection to healthcare providers and reduce sample collection time and cost. Until recently, the sensitivity and accuracy of the data obtained using saliva specimens for SARS-CoV-2 detection was controversial. However, recent clinical research has found that sensitive and reliable data can be obtained from saliva specimens using RT-qPCR, with approximately 81% to 95% correspondence with the data obtained from NP and OP swabs. These data suggest that self-collected saliva is an alternative option for the diagnosis of COVID-19.

• 한국기록관리학회지
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• 제17권4호
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• pp.51-86
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• 2017

본 연구는 해외 기록관에서 소장하고 있는 기록을 대상으로 시민들의 참여를 통해 전사를 수행하는 사례를 분석함에 있어 이와 관련된 개념적 논의를 조사하고 사례분석을 통해 운영현황 및 시사점을 논의하는 것을 목적으로 하였다. 개념적 논의에서는 시민 아키비스트, 참여형 아카이브, 크라우드소싱의 세 가지 개념을 바탕으로 시민 참여 전사 프로그램의 특징과 의의를 살펴보았다. 사례분석을 위해 미국과 영국, 호주에서 성공적으로 운영되고 있는 전사 프로그램 5개를 분석 대상으로 선정하였으며 기관 및 목적, 전사 대상 기록, 참여 관리, 인프라, 정책 및 가이드라인, 평가의 6가지 측면에서 분석을 수행하였다. 이러한 전사 프로그램들은 역사기록의 접근 향상이라는 구체적인 목표를 가지고 기관의 조율에 의해 운영되므로 자발적인 참여에 의해 운영되는 공동체 아카이브와는 차이가 있다. 따라서 시민들의 지속적인 참여 유도를 위한 방안을 마련하는 것이 필요하다. 이러한 측면에서 국내의 유사한 서비스에 대한 시사점으로 전사 프로그램의 목적을 명확히 서술하고 전사 대상 기록 선정 시 이용자의 관심을 유도할 수 있는 주제, 기록생산자, 기록 유형을 고려하는 것이 필요함을 제안하였다. 또한 기여도가 높은 소수의 참여자들과 파트너십을 형성할 것과 참여자 요구에 부합하는 전사 인터페이스 및 과업을 제시할 것을 제안하였다. 이와 더불어 간결하고 효과적인 가이드라인 및 전사 결과물 활용에 대한 정책을 제시하고 전사 결과물을 기록 검색 및 접근에 적극적으로 활용함으로써 참여자들의 기여를 가시적으로 인정하는 것이 필요함을 제안하였다.

• Molecules and Cells
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• 제46권12호
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• pp.727-735
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• 2023

Stem cells require high amounts of energy to replicate their genome and organelles and differentiate into numerous cell types. Therefore, metabolic stress has a major impact on stem cell fate determination, including self-renewal, quiescence, and differentiation. Lysosomes are catabolic organelles that influence stem cell function and fate by regulating the degradation of intracellular components and maintaining cellular homeostasis in response to metabolic stress. Lysosomal functions altered by metabolic stress are tightly regulated by the transcription factor EB (TFEB) and TFE3, critical regulators of lysosomal gene expression. Therefore, understanding the regulatory mechanism of TFEB-mediated lysosomal function may provide some insight into stem cell fate determination under metabolic stress. In this review, we summarize the molecular mechanism of TFEB/TFE3 in modulating stem cell lysosomal function and then elucidate the role of TFEB/TFE3-mediated transcriptional activity in the determination of stem cell fate under metabolic stress.

• Asian Pacific Journal of Cancer Prevention
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• 제14권4호
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• pp.2265-2268
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• 2013

Several lines of evidence indicate that cancer is a multistep process. To survey the mechanisms involving gene alteration and miRNAs in adrenocortical cancer, we focused on transcriptional factors as a point of penetration to build a regulatory network. We derived three level networks: differentially expressed; related; and global. A topology network ws then set up for development of adrenocortical cancer. In this network, we found that some pathways with differentially expressed elements (genetic and miRNA) showed some self-adaption relations, such as EGFR. The differentially expressed elements partially uncovered mechanistic changes for adrenocortical cancer which should guide medical researchers to further achieve pertinent research.

• Clinical and Experimental Pediatrics
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• 제53권8호
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• pp.786-789
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• 2010

Induced pluripotent stem (iPS) cells are generated by epigenetic reprogramming of somatic cells through the exogenous expression of transcription factors. Recently, the generation of iPS cells from patients with a variety of genetic diseases was found to likely have a major impact on regenerative medicine, because these cells self-renew indefinitely in culture while retaining the capacity to differentiate into any cell type in the body, thereby enabling disease investigation and drug development. This review focuses on the current state of iPS cell technology and discusses the potential applications of these cells for disease modeling; drug discovery; and eventually, cell replacement therapy.

• Bulletin of the Korean Chemical Society
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• 제26권1호
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• pp.34-35
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• 2005

• Asian Pacific Journal of Cancer Prevention
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• 제15권23호
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• pp.10355-10361
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• 2015

Background: MicroRNAs (miRNAs) are small non-coding RNA molecules found in multicellular eukaryotes which are implicated in development of cancer, including cutaneous squamous cell carcinoma (cSCC). Expression is controlled by transcription factors (TFs) that bind to specific DNA sequences, thereby controlling the flow (or transcription) of genetic information from DNA to messenger RNA. Interactions result in biological signal control networks. Materials and Methods: Molecular components involved in cSCC were here assembled at abnormally expressed, related and global levels. Networks at these three levels were constructed with corresponding biological factors in term of interactions between miRNAs and target genes, TFs and miRNAs, and host genes and miRNAs. Up/down regulation or mutation of the factors were considered in the context of the regulation and significant patterns were extracted. Results: Participants of the networks were evaluated based on their expression and regulation of other factors. Sub-networks with two core TFs, TP53 and EIF2C2, as the centers are identified. These share self-adapt feedback regulation in which a mutual restraint exists. Up or down regulation of certain genes and miRNAs are discussed. Some, for example the expression of MMP13, were in line with expectation while others, including FGFR3, need further investigation of their unexpected behavior. Conclusions: The present research suggests that dozens of components, miRNAs, TFs, target genes and host genes included, unite as networks through their regulation to function systematically in human cSCC. Networks built under the currently available sources provide critical signal controlling pathways and frequent patterns. Inappropriate controlling signal flow from abnormal expression of key TFs may push the system into an incontrollable situation and therefore contributes to cSCC development.

• BMB Reports
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• 제51권2호
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• pp.92-97
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• 2018

B cell leukemia/lymphoma 3 (Bcl3) plays a pivotal role in immune homeostasis, cellular proliferation, and cell survival, as a co-activator or co-repressor of transcription of the $NF-{\kappa}B$ family. Recently, it was reported that Bcl3 positively regulates pluripotency genes, including Oct4, in mouse embryonic stem cells (mESCs). However, the role of Bcl3 in the maintenance of pluripotency and self-renewal activity is not fully established. Here, we report the dynamic regulation of the proliferation, pluripotency, and self-renewal of mESCs by Bcl3 via an influence on Nanog transcriptional activity. Bcl3 expression is predominantly observed in immature mESCs, but significantly decreased during cell differentiation by LIF depletion and in mESC-derived EBs. Importantly, the knockdown of Bcl3 resulted in the loss of self-renewal ability and decreased cell proliferation. Similarly, the ectopic expression of Bcl3 also resulted in a significant reduction of proliferation, and the self-renewal of mESCs was demonstrated by alkaline phosphatase staining and clonogenic single cell-derived colony assay. We further examined that Bcl3-mediated regulation of Nanog transcriptional activity in mESCs, which indicated that Bcl3 acts as a transcriptional repressor of Nanog expression in mESCs. In conclusion, we demonstrated that a sufficient concentration of Bcl3 in mESCs plays a critical role in the maintenance of pluripotency and the self-renewal of mESCs via the regulation of Nanog transcriptional activity.