• 한국잡초학회지
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• 제14권4호
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• pp.280-290
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• 1994

작물(作物)과 잡초(雜草)에서 Oxyfluorfen과 Chlormethoxynil에 대한 선택성(選擇性)과 살초력(殺草力)을 비교(比較)하고자 발아실험(發芽實驗), 수경재배실험(水耕栽培實驗), 두가지 토양(土壤)에서의 발아전(發芽前) 토양처리실험(土壤處理實驗)을 실시(實施)하였으며, 처리부위(處理部位)와 시간(時間)을 다르게 하였을 때의 초종간(草種間) 흡수(吸收) 및 이행(移行)을 조사하고자 $^{14}C$-oxyfluorfen을 이용(利用)한 흡수이행실험(吸收移行實驗)을 실시(實施)하였다. 1. 발아실험(發芽實驗), 수경재배실험(水耕栽培實驗), 발아전(發芽前) 토양처리실험(土壤處理實驗)을 통하여 볼 때 벼, 옥수수에서는 공시제초제(供試除草劑) 농도(濃度)가 증가(增加)되면서 약간 생장(生長)이 억제(抑制)되는 경향(傾向)을 나타냈으나 감수성(感受性) 초종(草種)인 참소리쟁이, 바랭이에서는 농도(濃度)가 높아짐에 따라 살초력(殺草力)이 증대(增大)되어 생장억제(生長抑制)가 심하였으며 전반적(全般的)으로 Oxytluorfen처리(處理)는 Chlormethoxynil처리(處理)보다 생장억제(生長抑制)가 심하였다. 2. 발아기간(發芽期間)동안의 생육억제(生育抑制) 효과(效果)를 나타낸 것을 보면 발아과정(發芽過程) 때보다 발아후(發芽後) 생육과정(生育過程)에서 많은 것으로 사료(思料)되며, 벼와 옥수수에서 가장 둔감(鈍感)한 반응(反應)을, 피에서는 비교적 둔감(鈍感)한 반응(反應)을, 참소리쟁이와 바랭이에서는 가장 민감(敏感)한 반응(反應)을 나타내었다. 3. 수경재배실험(水耕栽培實驗) 결과(結果), 2엽기(葉期)에서의 처리(處理)가 4엽기(葉期)에서의 처리(處理)보다 생장억제(生長抑制)가 더 심하게 나타났으며 지상부(地上部)보다 지하부(地下部)가 많은 생장억제(生長抑制)를 나타내었다. 4. 발아전(發芽前) 토양처리실험(土壤處理實驗) 결과(結果), 유기물(有機物), C.E.C. 및 점토성분(粘土成分)이 적은 사양토(砂壤土)에서 생장억제(生長抑制)가 심하였으며 초종간(草種間)에는 참소리쟁이와 바랭이에서 많이 나타났다. 5. $^{14}C$-oxytluorfen의 흡수(吸收)는 작물(作物)과 잡초(雜草)에서 모두 잘 되였으나 이행은 모든 초종(草種)에서 거의 되지 않는 경향이었으나 시간(時間)이 경과(經過)함에 따라 감수성(感受性) 초종(草種)인 참소리쟁이와 바랭이에서 흡수량(吸收量)이 많아졌으며 작물(作物)과 잡초간(雜草間) 선택성(選擇性)은 주로 흡수(吸收)된 양(量)에 따라 결정된다고 생각한다.

• 생태와환경
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• 제42권1호
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• pp.75-84
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• 2009

여러 가지 은나노물질(SNPs)의 M. aeruginosa 생장에 대한 영향을 실내, 외 실험을 통해 조사하였다. 제조된 4가지 SNPs는 농도 $200mg\;L^-1$, 입자크기 $20{\sim}40nm$, 갈색 Ag로서의 수용액이었으며 이 용액들을 각각 실험에 사용하였다. SNPs는 unicellular M. aeruginosa에 대하여 0.01, $0.1mg\;L^-1$의 첨가농도에서 각각 99.4%, 99.9%의 조류 생장억제 효과를 나타냈으며, colonial M. aeruginosa에 대하여는 여러 가지 농도 중 $1mg\;L^-1$의 농도에서 가장 높은 생장억제 효과(98.5%)를 나타냈다. 더욱이 부영양화한 현장에서 SNPs를 첨가한 enclosure 실험을 통해 M. aeruginosa에 대한 선택적 제어 가능성이 시사되었다. 본 연구를 통해서 향후 SNPs를 더욱 보완하면 M. aeruginosa를 비롯한 유해 남조의 선택적 제어에 좀더 효과적일 것으로 판단된다.

• 대한미생물학회지
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• 제11권1호
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• pp.33-48
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• 1976

The practical significance of using a selective enrichment procedure for detecion and enumeration of salmonella is well recognized. There are still various selective enrichment media has been communly used. Early years selenite broth was recomnended as an enrichment media for the isolating of salmonella. Hajna introduced a modified tetrathionate broth and demonstrated the greater efficiency to compare with the previous enrichment media. Raj also described that the new medium called dulcitol selenite enrichment and has been found to be very satisfactory, especially general implication in food poisoning. Authors tried to compare these 3 enrichment media for isolating salmonella. 1. When salmonella strains were inoculated $1{\sim}10^6$ cells per tube to these 3 enrichment media, mostly similar results were obtained between selenite broth and DS broth. In these 2 enrichment broth were showed $10^7/ml-10^8/ml$ cells of all tested salmonella strains. But in the case of TT broth it was found that the growth was $10^3/ml{\sim}10^4/ml$ cells for tested strain. 2. When E. coli, Proteus, Citrobacter were inoculate $10{\sim}10^6$ cells per tube to these 3 enrichment media. It was suggested that DS broth was showed more inhibitory action than that of selenite broth. TT broth showed high inhibition to these 3 organisms tested. 3. It was generally known that the incubation time is influenced to the frequency of salmonella detection. For this tendency, DS broth and selenite broth were showed similar results within 24 hrs to 48hrs incubation to the test. But DS broth showed more inhibitory action to E. coli and Proteus than that of selenite broth. 4. When $1{\sim}10$ cells were inoculated(per tube) to these 3 enrichment media, DS broth was found to be more sensitive than that of selenite broth.

• Fisheries and Aquatic Sciences
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• 제19권8호
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• pp.33.1-33.7
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• 2016

Antifreeze proteins (AFPs) lower the freezing point but not the melting point of aqueous solutions by inhibiting the growth of ice crystals via an adsorption-inhibition mechanism. However, the function of type IV AFP (AFP IV) is questionable, as its antifreeze activity is on the verge of detectable limits, its physiological concentration in adult fish blood is too low to function as a biological antifreeze, and its homologues are present even in fish from tropic oceans as well as freshwater. Therefore, we speculated that AFP IV may have gained antifreeze activity not by selective pressure but by chance. To test this hypothesis, we cloned, expressed, and assayed AFP IV from cultured subtropical olive flounder (Paralichthys olivaceus), which do not require antifreeze protein for survival. Among the identified expressed sequence tags of the flounder liver sample, a 5'-deleted complementary DNA (cDNA) sequence similar to the afp4 gene of the longhorn sculpin was identified, and its full-length cDNA and genome structure were examined. The deduced amino acid sequence of flounder AFP IV shared 55, 53, 52, and 49 % identity with those of Pleuragramma antarcticum, Myoxocephalus octodecemspinosus, Myoxocephalus scorpius, and Notothenia coriiceps, respectively. Furthermore, the genomic structure of this gene was conserved with those of other known AFP IVs. Notably, the recombinant AFP IV showed a weak but distinct thermal hysteresis of $0.07{\pm}0.01^{\circ}C$ at the concentration of 0.5 mg/mL, and ice crystals in an AFP IV solution grew star-shaped, which are very similar to those obtained from other polar AFP IVs. Taken together, our results do not support the hypothesis of evolution of AFP IV by selective pressure, suggesting that the antifreeze activity of AFP IV may have been gained by chance.

• Journal of Animal Science and Technology
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• 제46권6호
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• pp.999-1006
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• 2004

NPC agar， DHL agar, MacConkey agar와 Cetrimide desoxycholate agar의 선택성을 조사 하였다". Cetrimide desoxycholate agarz 가 다른 선택성 배지에 비해 그람양성 세균을 억제하고 Pseudomonas를 비롯한 내냉성 그람음성 세균의 선택적 배양에 더 좋은 한천배지이었다. Resazurin 환원시간 검시에서 Cetrimide desoxycholate broth를 시유와 혼합하여 $21^{\circ}C$ 에 서 18 시간 배양한 후 resazurin을 첨가하여 변색되는 시간으로 2차오염 을 결정하는 방법으로서 조사하였다. Resazurin 환원시간 검사와 저장성 검사간에 81%의 관련성이 있었다. 저장성 검사의 우유에서 Pseudomonas가 가장 먼도 높게 분리되었으며 다음으로 Acinetobacter와 Aeromonas가 검출되었다 Resazurin 환원시간 검사에는 Acinetobacter, Pseudomonas, Enterobacter의 순서의 빈도로 검출되었다.

• Journal of Acupuncture Research
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• 제23권2호
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• pp.91-102
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• 2006

Objectives : Ulmus davidiana Planch (UD) has long been known to have anti-inflammatory and protective effects on damaged tissue, inflammation and bone among other functions. Methods : This study was undertaken to address whether the water extract of the bark of UD could modulate proliferation of mouse osteoclasts in vitro and to investigate its effect on cyclooxygenase-2 (COX-2), which converts arachidonic acid to prostaglandin E2 (PGE2) and is highly expressed in osteoclasts. Mouse osteoclasts were tested in vitro for growth inhibition, proliferation cell nuclear antigen expression, and COX-2 activity and expression after treatment with UD extract. Results : Its effects were compared with those of indomethacin (a nonselective COX inhibitor) and celecoxib (a selective COX-2 inhibitor) by Cell viability assay, Cell cycle analysis, Immunohistochemical analysis of PCNA expression, Western blot analysis and PGE2 Enzyme immunoassay (EIA). UD demonstrated a strong growth inhibitory action in both tested osteoclasts cells. The IC50s were $10\;{\mu}g/ml$ for UD, $6\;{\mu}M$ for celecoxib and $42\;{\mu}M$ for indomethacin. UD, as well as celecoxib and indomethacin, suppressed proliferation cell nuclear antigen expression and PGE2 synthesis in osteoclasts. UD inhibited COX-2 expression, whereas celecoxib inhibited COX-2 activity directly. Conclusion : UD selectively and effectively inhibits osteoclasts cell growth in vitro. Inhibitory action of PGE2 synthesis via suppression of COX-2 expression may be responsible for its anti-inflammatory activity.

• Journal of Microbiology and Biotechnology
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• 제13권4호
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• pp.607-612
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• 2003

To elucidate the action mechanism of MCS-C2, a novel analogue of toyocamycin and sangivamycin, its effect on the expression of cell cycle-related proteins in the human myelocytic leukemia cell line HL-60 was examined using Western blotting and a flow cytometric analysis. MCS-C2, a selective inhibitor of cyclin-dependent kinases, was found to inhibit cell growth in a time- and dose-dependent manner, and inhibits cell cycle progression by inducing the arrest at G1 and G2/M phases, in HL-60 cells. The flow cytometric analysis revealed an appreciable arrest of cells in the G2/M phase of the cell cycle after treatment with MCS-C2. The HL-60 cell population increased gradually from 13% at 0 h, to 28% at 12 h in the G2/M phase, after exposure to $2{\;}\mu\textrm{M}$ MCS-C2. Furthermore, Western blot analysis demonstrated that MCS-C2 induced the cell cycle arrest at G1 phase through the inhibition of pRb phosphorylation. Hypophosphorylated pRb accumulated after treatment with $5{\;}\mu\textrm{M}$ MCS-C2 for 12 h, whereas, the level of hyperphosphorylated pRb was reduced. Thus, treatment of the cell with MCS-C2 suppressed the hyperphosphorylated form of pRb with a commensurate increase in the hypophosphorylated form.

• The Korean Journal of Physiology and Pharmacology
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• 제15권4호
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• pp.203-210
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• 2011

Cilostazol is a selective inhibitor of phosphodiesterase 3 that increases intracellular cAMP levels and activates protein kinase A, thereby inhibiting vascular smooth muscle cell (VSMC) proliferation. We investigated whether AMP-activated protein kinase (AMPK) activation induced by heme oxygenase-1 (HO-1) is a mediator of the beneficial effects of cilostazol and whether cilostazol may prevent cell proliferation and reactive oxygen species (ROS) production by activating AMPK in VSMC. In the present study, we investigated VSMC with various concentrations of cilostazol. Treatment with cilostazol increased HO-1 expression and phosphorylation of AMPK in a dose- and time-dependent manner. Cilostazol also significantly decreased platelet-derived growth factor (PDGF)-induced VSMC proliferation and ROS production by activating AMPK induced by HO-1. Pharmacological and genetic inhibition of HO-1 and AMPK blocked the cilostazol-induced inhibition of cell proliferation and ROS production.These data suggest that cilostazol-induced HO-1 expression and AMPK activation might attenuate PDGF-induced VSMC proliferation and ROS production.

• 대한한의학방제학회지
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• 제13권2호
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• pp.111-123
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• 2005

The purpose of this study was to investigate the anticancer effects of Caesalpiniae Lignum (Somok) on HepG2 cells, a human liver cancer cell line. To study the cytotoxic effect of Caesalpiniae Lignum methanol extract (CL-MeOH) on HepG2 cells, the cells were treated with various concentrations of CL-MeOH and then cell viability was determined by XTT reduction method and trypan blue exclusion assay. CL-MeOH reduced proliferation of HepG2 cells in a dose-dependent manner. To confirm the induction of apoptosis, HepG2 cells were treated with various concentrations of CL-MeOH. The activation of caspase 3 and the cleavage of poly ADP-ribose polymerase (PARP), a substrate for caspase-3 and a typical sign of apoptosis, was examined by western blot analysis. CL-MeOH decreased procaspase 3 level in a dose-dependent manner and induced the clevage of PARP at concentration> $200{\mu}/ml$. Mitogen-activated protein (MAP) kinase signaling cascades are multi-functional signaling networks that influence cell growth, differentiation, apoptosis, and cellular responses to stress. CL-MeOH-induced MAPK activation was examined by Western blot for phosphorylated ERK, p38 and JNK. CL-MeOH significantly increased p38 phosphorylation and JNK phosphorylation in a dose-dependent manner. Inhibition of p38 function using the selective inhibitor SB20358O results in inhibition of apoptosis by CL-MeOH. These results suggest that CL-MeOH-induced apoptosis is MAP kinase-dependent apoptoric pathway. These results suggest that CL-MeOH is potentially useful as a chemotherapeutic agent in human liver cancer.

• Natural Product Sciences
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• 제10권6호
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• pp.296-301
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• 2004

Previously, a flavonoid fraction, which consisted mainly of protocatechuic acid, fustin, fisetin, sulfuretin, and butein, here named RCMF $({\underline{R}}VS\;{\underline{c}}hloroform-{\underline{m}}ethanol\;{\underline{f}}raction)$, was prepared from a crude acetone extract of Rhus verniciflua Stokes (RVS) which is traditionally used as a food additive and as an herbal medicine. In this study, we evaluated the effects of RCMF on the antioxidant defense system using embryonic normal hepatic cell line (BNL CL.2) and its SV40-mediated transformed cell line (BNL SV A.8). This study demonstrates that RCMF selectively stimulated the antioxidant defense system of normal cells, as BNL CL.2 cells proved to be more sensitive to RCMF-mediated increases of superoxide dismutase, catalase, glutathione, and glutathione reductase than BNL SV A.8 cells. In particular, RCMF caused a significant increase in the malonaldehyde content of BNL SV A.8 cells, which is believed to be closely associated with cytotoxicity of RCMF and RCMF-mediated growth inhibition. Collectively, our findings suggest that the flavonoid fraction, RCMF, selectively stimulates the antioxidant defense system in normal rather than hepatic tumor cells.