• Title/Summary/Keyword: Selected Lines

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Cold Tolerance Characteristic Test of High Yield Tongil-type Rice Breeding Lines for Processing

  • Kang-Su Kwak;Sea-Kwan Oh;Kuk-Hyun Jung;Dae-Ha Seo
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2022.10a
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    • pp.278-278
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    • 2022
  • Recently, the yield potential of high yield Tongil-type rice varieties has greatly increased, reaching 817kg/10a(Geumgang No.1). Moreover, in order to promote rice consumption and strengthen the competitiveness of the rice processing industry, the R&D of high yield Tongil-type rice varieties for each processing purpose, such as rice noodles, grain feed and industrial materials, has been continuously carried out. However, because Tongil-type rice varieties or lines are generally very vulnerable to cold damage, cold tolerance test can be said to be absolutely necessary to improve the cultivation safety. This study is the result of the seedling and field cold tolerance characteristic tests carried out in 2021 of high yield Tongil-type rice breeding lines. For the cold tolerance characteristic test of seedlings, total 303 high yield rice breeding lines for processing were treated in cold water of 13℃ and irrigation depth of 4cm for 10 days from the third-leaf age, then it was evaluated by comparing the degree of discoloration and withering with the checked varieties(Boramchan, Hanahreum No.2). Also, for the test of field, total 186 high yield rice breeding lines for processing were treated in cold water of 17℃ by keeping pouring day and night from 30 days after transplanting to ripening stage, then it was evaluated by comparing the degree of discoloration, delay of heading, shortening rate of stem length and percent of fertile grain etc. with the checked varieties. And the cold tolerance evaluative criteria were classified as strong(1~3), medium(4~6) and weak(7~9) in overall cold tolerance. (Seedling test) As for the degree of cold tolerance of the check variety, 'Boramchan' and 'Hanahreum No.2' showed a response of 'medium' and 'weak', respectively. However, there was no 'strong' line in the high yield rice breeding lines, 2 lines showed a 'medium' response, and 301 lines showed a 'weak' response. Therefore, except for a few lines(0.7%), most lines(99.3%) showed a 'weak' response. (Field test) In terms of the overall cold tolerance of the check variety, both 'Boramchan' and 'Hanahreum No.2' showed a 'medium' response. Similarly, there was no 'strong' line in the case of high yield rice breeding lines, 20 lines showed a 'medium' response, and 166 lines showed a 'weak' response. Therefore, except for some(10.8%) lines, most(89.2%) lines showed a 'weak' response. From the above results, we selected about 100 individuals with less seed shattering and degeneration of the ear tip, and with a relatively high percent of fertile grain, and are continuing to select lines with improved cold tolerance in the F4~F5 group in this year. As such, most of the Tongil-type rice varieties have poor cold tolerance and thus have low cultivation safety at low temperatures. However, it is important to select improved lines through generational progress because there are some lines that still have a certain level of cold tolerance among them.

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Growth Performance of Offspring from Selected Korean, Cultured Japanese and Their Reciprocal Intraspecific Hybrids of Red Sea Bream, Pagrus major

  • Noh, Choong-Hwan;Hong, Kyung-Pyo;Myoung, Jung-Goo;Kim, Jong-Man
    • Proceedings of the Korean Aquaculture Society Conference
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    • 2003.10a
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    • pp.37-37
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    • 2003
  • Intraspecific hybrids have been proven to be a useful tool for increase growth rate in some freshwater fish species. However the effects of intraspecific hybrids in seawater fish species is little known. To assess whether intraspecific hybrids in red sea bream was associated with growth enhancement, growth performances of offspring from two inbred lines, selected Korean line (KORDI-F4) and cultured Japanese line (IPN) and two intraspecific hybrids lines (KORDI-F4♀×JPN♂ and JPN♀×KORDI-F4♂) of red sea bream were compared. There's no significant difference in body weight between offspring groups during seed production period (until 4 months old). At seven months old (after three months rearing trials on sea cages communally), the offspring from KORDI-F4 (41.0±11.1g) and JPN♀×KORDI-F4♂ (39.3±8.1g) showed significantly better performance in body weight than that of the offspring from KORDI-F4♀×JPN♂ (37.0±8.1g) and IPN (35.7±9.7g). At 15 months old, the offspring from JPN♀×KORDI-F4♂ showed best growth performances in body weight, weight gain, specific growth rate among four offspring groups. As the results, the intraspecific hybrids between cultured Japanese line and selected Korean line showed superior growth performances than their paternal and maternal inbred lines.

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Extended Three Region Partitioning Method of Loops with Irregular Dependences (비규칙 종속성을 가진 루프의 확장된 세지역 분할 방법)

  • Jeong, Sam-Jin
    • Journal of the Korea Convergence Society
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    • v.6 no.3
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    • pp.51-57
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    • 2015
  • This paper proposes an efficient method such as Extended Three Region Partitioning Method for nested loops with irregular dependences for maximizing parallelism. Our approach is based on the Convex Hull theory, and also based on minimum dependence distance tiling, the unique set oriented partitioning, and three region partitioning methods. In the proposed method, we eliminate anti dependences from the nested loop by variable renaming. After variable renaming, we present algorithm to select one or more appropriate lines among given four lines such as LMLH, RMLH, LMLT and RMLT. If only one line is selected, the method divides the iteration space into two parallel regions by the selected line. Otherwise, we present another algorithm to find a serial region. The selected lines divide the iteration space into two parallel regions as large as possible and one or less serial region as small as possible. Our proposed method gives much better speedup and extracts more parallelism than other existing three region partitioning methods.

A New Yellow Graft Cactus(Gymnocalycium mihanovichii × marsoneri) Cultivar 'Hangseong' (노란색 비모란 종간교잡종 '황성' 육성)

  • Jeong, Myeong Il;Chung, Bong Nam;Park, Pil Man
    • FLOWER RESEARCH JOURNAL
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    • v.16 no.4
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    • pp.321-324
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    • 2008
  • A new yellow graft cactus Gymnocalycium mihanovichii ${\times}$ marsoneri cv. 'Hangseong' was developed by the National Institute of Horticultural &Herbal Science, Rural Development Administration in 2005. 'Hangseong' was selected from a interspecific crossing between Gymnocalycium mihanovichii and G. marsoneri. In 1998, 2 lines of '9834024' and '9834036' were selected from a crossing between G. mihanovichii 'IG-124' and G. marsoneri 'IG-223'. The 2 lines were crossed between them. In 2001, progenies produced from a crossing of the 2 lines were nursed and, finally yellow colored 'Wonkyo G1-169' was selected through 3 times of characteristic trials. It was named as 'Hangseong' and applied for a variety protection. Characteristics of 'Hangseong' is as follows: The color of body and tubercles is yellow. The shape of glove is flat with 7 to 9 deep ribs. Spine is medium straight with long size and brown color. Growth is fast as to reach 49.5 mm in diameter at 10 month after planting. Propagation ability is good as to set as many as 7.2 tubercles. 'Hangseong' is suitable for export to European countries or America. Characteristics of the cultivar can be maintained by vegetative propagation.

요소회로 효소 유전자로 형질전환 된 Chinese Hamster Ovary 세포의 암모니아 제거능력과 세포성장률

  • Kim, Hong-Jin;Jeong, Myeong-Il;Jang, Yun-Jeong;Im, Mi-Hui;Kim, Ik-Hwan;Kim, Ik-Yeong
    • 한국생물공학회:학술대회논문집
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    • 2001.11a
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    • pp.66-69
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    • 2001
  • Previously we developed a CHO cell line (CHO-OTC1-A19) expressing the first two enzymes of urea cycle. This cell line showed higher ammonia removal activity and faster growth rate than the vector controlled CHO cells (CHO-neo-5). The purpose of this study was to develop a cell line with higher ammonia removal activity than the cell line developed previously. To accomplish this, we constructed stable CHO cell lines expressing the first three, the first four, or all five enzymes of urea cycle by the stable transfection method. We finally selected CHO-AL-19 cell line expressing the first three, the first four enzymes of the cycle with higher ammonia activity than CHO-OTC1-A19 and CHO-n대-5 cell lines: 40% and 15% higher than those of CHO-neo-5 and CHO-OTC1-A19 cell lines 72 hour after culture started, respectively. It also showed 44% and 10% higher cell viability than CHO-neo-5 and CHO- OTC1-A19 cell lines at higher cell density. In addition, CHO-AL-19 cells showed 45%-60% and about 20% lower ammonia concentration per cell than those of CHO-neo-% and CHO-OTC1-A19 cell lines, respectively. These results indicate that CHO-AL-19 could be used in the production of human therapeutic proteins with higher efficiency.

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Downregulation of ARFGEF1 and CAMK2B by promoter hypermethylation in breast cancer cells

  • Kim, Ju-Hee;Kim, Tae-Woo;Kim, Sun-Jung
    • BMB Reports
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    • v.44 no.8
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    • pp.523-528
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    • 2011
  • To identify novel genes that are regulated by promoter methylation, a combinational approach involving in silico mining followed by molecular assay was performed. From the expression microarray data registered in the European bioinformatics institute (EBI), genes showing downregulation in breast cancer cells were initially screened and then selected by e-Northern analysis using the Unigene database. A series of these in silico methods identified CAMK2B and ARFGEF1 as candidates, and the two genes were revealed to be hypermethylated in breast cancer cell lines and hypomethylated in normal breast cell lines. Additionally, cancer cell lines showed downregulated expression of these genes. Furthermore, treatment of the cancer cell lines with a demethylation agent, 5-Aza-2'-deoxycytidine, recovered expression of CAMK2B and ARFGEF1, implying that hypermethyaltion silenced gene activity in cancer cells. Taken together, promoter methylations of CAMK2B and ARFGEF1 are novel epigenetic markers identified in breast cancer cell lines and can be utilized for the application to clinical cancer tissues.

Production of transgenic Alstroemeria plants containing virus resistance genes via particle bombardment

  • Kim, Jong Bo
    • Journal of Plant Biotechnology
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    • v.47 no.2
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    • pp.164-171
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    • 2020
  • Transgenic Alstroemeria plants resistant to Alstroemeria mosaic virus (AlMV) were generated through RNA-mediated resistance. To this end, the friable embryogenic callus (FEC) of Alstroemeria was induced from the leaf axil tissue and transformed with a DNA fragment containing the coat protein gene and 3'-nontranslated region of AlMV through an improved particle bombardment system. The bar gene was used as a selection marker. More than 300 independent transgenic FEC lines were obtained. Among these, 155 lines resistant to phosphinothricin (PPT) were selected under low stringent conditions. After increasing the stringency of PPT selection, 44 transgenic lines remained, and 710 somatic embryos from these lines germinated and developed into shoots. These transgenic shoots were then transferred to the greenhouse and challenged with AlMV. In total, 25 of the 44 lines showed some degree of resistance. PCR analysis confirmed the presence of the viral sequence. Virus resistance was observed at various levels. Establishment of an efficient transformation system for Alstroemeria will allow inserting transgenes into this plant to confer resistance to viral and fungal pathogens. Accordingly, this is the first report on the production of a transgenic virus-resistant Alstroemeria and lays the foundation for alternative management of viral diseases in this plant.

Transcriptional activation of anthocyanin structural genes in torenia cv. Kauai rose by overexpression of anthocyanin regulatory transcription factors

  • Xu, Jun-Ping;Naing, Aung Htay;Kim, Chang-Kil
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2018.10a
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    • pp.33-33
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    • 2018
  • This study was conducted to examine the role of the transcription factors (TFs) (RsMYB1 and mPAP1+B-Peru) in the regulation of anthocyanin biosynthesis in the ornamental torenia cv. Kauai rose. In this study, we could produce several putative transgenic lines overexpressing the TFs via Agrobacterium-mediated transformation, and presence of the TFs in the randomly selected five transgenic lines was confirmed using polymerase chain reaction (PCR). According to results of reverse transcription-PCR analysis (RT-PCR), the expression of the TFs in all transgenic lines and of the anthocyanin structural genes (CHS, F3H, DFR, and ANS) in all transgenic lines and WT plants were distinctly detectable. However, transcript levels of the structural genes expressed in the transgenic lines overexpressing TFs were significantly higher than those expressed in WT plants. Therefore, it is suggested that anthocyanin content in flowers of the transgenic torenia would be significantly higher than that in flowers of WT plants. Moreover, these results indicate that the TFs (RsMYB1 and mPAP1+B-Peru) could be exploited as potential anthocyanin regulatory TFs to enhance anthocyanin content in the other horticultural plants.

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Evaluation and Selection of Breeding Lines of Pepper Developed by Incorporation of Resistance to Phytophthora capsid into Local Cultivars of Gyeungbuk Province (고추 역병 저항성 도입 경북지역 재래종 육성계통의 평가와 선발)

  • Kim, Byung-Soo;Kim, Jeong-Hoon
    • Current Research on Agriculture and Life Sciences
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    • v.21
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    • pp.11-16
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    • 2003
  • Lines bred for resistance to Phytophthora capsici by incorporation of resistance to P. capsici in PI201234 or CM334 into 'Subi' and 'Chilseong', land races in Youngyang, and 'Punggak', a land race in Cheongdo in Gyeungbuk province, and lines bred for fortification of one of them above with resistance to viral complex, and tolerant selections from another landrace collection from Punggak (KC268) were evaluated for resistance to P. capsici by inoculation at seedling stage. Almost all the breeding lines showed high level of resistance to P. capsid and selections from KC268 showed tolerance or moderate resistance to P. capsid. The selected plants were grown in a net cage in an outdoor nursery for seed production. Utilization of the lines in breeding was discussed.

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Normal range of facial asymmetry in spherical coordinates: a CBCT study

  • Yoon, Suk-Ja;Wang, Rui-Feng;Na, Hee Ja;Palomo, Juan Martin
    • Imaging Science in Dentistry
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    • v.43 no.1
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    • pp.31-36
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    • 2013
  • Purpose: This study aimed to measure the bilateral differences of facial lines in spherical coordinates from faces within a normal range of asymmetry utilizing cone-beam computed tomography (CBCT). Materials and Methods: CBCT scans from 22 females with normal symmetric-looking faces (mean age 24 years and 8 months) were selected for the study. The average menton deviation was $1.01{\pm}0.66$ mm. The spherical coordinates, length, and midsagittal and coronal inclination angles of the ramal and mandibular lines were calculated from CBCT. The bilateral differences in the facial lines were determined. Results: All of the study subjects had minimal bilateral differences of facial lines. The normal range of facial asymmetry of the ramal and mandibular lines was obtained in spherical coordinates. Conclusion: The normal range of facial asymmetry in the spherical coordinate system in this study should be useful as a reference for diagnosing facial asymmetry.