• Natural Product Sciences
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• 제5권4호
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• pp.165-171
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• 1999

Since reactive oxygen species (ROS) play an important role in carcinogenesis and other several human diseases, antioxidants present in consumable fruits, vegetables, and beverages have received considerable attention as cancer chemopreventive agents. Thus, in order to identify antioxidants in plant extracts, potential activity was assessed by determining with inhibition of a xanthine/xanthine oxidase assay system. Approximately 170 plant extracts of Korean herbal medicines were primarily evaluated for the potential of antioxidant activity. As a result, 13 plant extracts were found to be active $(IC_{50}$<$100\;{\mu}g/ml)$. Especially, Juncus effusus, Selaginella tamariscina, Pueraria thunbergiana and Sedum albroseum showed strong inhibitory activity in this process. Further studies for the identification of active principles from these active lead plant extracts might be warranted.

• Bulletin of the Korean Chemical Society
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• 제29권8호
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• pp.1479-1484
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• 2008

Protein tyrosine phosphatase (PTP) 1B is the superfamily of PTPs and a negative regulator of multiple receptor tyrosine kinases (RTKs). Inhibition of protein tyrosine phosphatase 1B (PTP1B) has been proposed as a strategy for the treatment of type 2 diabetes and obesity. Recently, it has been reported that amentoflavone, a biflavonoid extracted from Selaginella tamariscina, inhibited PTP1B. In the present study, docking model between amentoflavone and PTP1B was determined using automated docking study. Based on this docking model and the interactions between the known inhibitors and PTP1B, we determined multiple pharmacophore maps which consisted of five features, two hydrogen bonding acceptors, two hydrogen bonding donors, and one lipophilic. Using receptor-oriented pharmacophore-based in silico screening, we searched the biflavonoid database including 40 naturally occurring biflavonoids. From these results, it can be proposed that two biflavonoids, sumaflavone and tetrahydroamentoflavone can be potent allosteric inhibitors, and the linkage at 5',8''-position of two flavones and a hydroxyl group at 4'-position are the critical factors for their allosteric inhibition. This study will be helpful to understand the mechanism of allosteric inhibition of PTP1B by biflavonoids and give insights to develop potent inhibitors of PTP1B.

• 생약학회지
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• 제33권1호통권128호
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• pp.35-41
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• 2002

The MeOH ext., $CH_2Cl_2$ Frac., EtOAc Frac., n-BuOH Frac., and $H_2O$ Frac. of 23 Korean medicinal herbs were prepared and were tested the inhibitory effects on Cytochrome P450 (Cyp) 1A1/2, 2B1/2, 2E1. Among the tested samples, the extracts of Selaginella tamariscina, Euonymus alatus, Salvia miltiorhiza, Angelica acutiloba, Rheum palmatum, Paeonia moutan, Scutellaria barbata, Tribulus terrestris, Hedyotis diffusa, Curcuma zedoaria, Rehmania glutinosa, Trogopterus xanthipes, Melandryum firmum, Achyranthes bidentata, Leonurus sibricus, Panax ginseng, Paeonia lactiflora, Poncirus trifoliata, Cnidium officinale, Cyperus rotundus, Corydalis ternata showed significant inhibitory effects on Cyp 1A1/2, 2B1/2, 2E1. The $IC_{50}$ values of those extracts were found to be below $50\;{\mu}g/ml$.

• 생약학회지
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• 제35권1호통권136호
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• pp.28-34
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• 2004

DNA damage induced by reactive oxygen species (ROS) seems to play an important role in the induction of mutation and cancer. Hydrogen peroxide $(H_2O_2)$ has been shown to induce a variety of genetic alterations, probably by the generation of hydroxyl radicals via Fenton reaction. In this study, we examined the ability of medicinal herbs in the suppression of $H_2O_2$-induced mutagenesis. Human fibroblast GM00637 cells were treated with $H_2O_2$ in the presence or absence of medicinal herbs, and $H_2O_2$-induced mutant frequency was measured at the hypoxanthine guanine phosphoribosyl transferase (HPRT) locus. Treatment of cells with various doses of $H_2O_2$ caused a significant increase of the HPRT mutant frequency. However, pretreatment of cells with several medicinal herbs reduced $H_2O_2$-induced mutant frequency. The strong antimutagenic effects were observed from the methylene chloride and ethyl acetate fractions of Selaginella tamariscina, Panax ginseng, and Angelica acutiloba; ethyl acetate fractions of Rehmania glutinosa, Leonurus sibiricus, Curcuma zedoaria and Commiphora molmol; butanol fractions of Scutellaria barbata, Tribulus terrestris, Curcuma zedoaria, Cyperus rotundus and Carthamus tinctorius, which were more than 60% inhibition of $H_2O_2$-induced mutant frequency at the HPRT locus.

• The Korean Journal of Ecology
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• 제23권3호
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• pp.217-221
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• 2000

본 조사지역은 경상남도 통영시 한산면 매죽리에 위치하고 있는 섬으로 매물도, 소매물도, 어유도로 구성된다. 본 조사는 1996년 9월부터 1997년 8월까지 수회에 걸쳐 식생을 조사하여 현존식생도와 군락조성표를 작성하였다. 본 조사지역의 식생은 동백나무군락(전형하위군락, 후박나무하위군락, 메밀잣밤나무하위군락, 쥐똥나무하위군락, 소사나무하위군락, 부처손하위군락, 곰솔하위군락), 곰솔군락, 사방오리식재림, 억새군락 등 1개의 식재림을 포함한 총 4개군락과 7개 하위군락으로 구분되었다. 본 조사지역인 어유도 북서사면의 동백나무군락, 동서사면의 부처손군락, 매물도의 정상부와 해안암벽부분, 소매물도의 해안암벽부분은 식생 이 가장 잘 보존되어 있다. 어유도의 정상부와 소매물도의 정상부는 염소의 방목으로 인한 답압형의 식생이 분포하고, 소매물도의 마을 뒷편의 능선부는 산화로 인해 식생이 많이 파괴된 것을 제외하고는 대체로 보존이 양호한 편이다.

• 한국자원식물학회지
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• 제20권2호
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• pp.185-192
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• 2007

8종의 식용 및 약용 양치식물에서 80% 에탄올을 이용하여 부위별 추출물을 조제한 후 각각의 생리활성물질 및 항산화활성을 조사하였다. 총 폴리페놀 함량은 쇠고비의 근경에서 $57.32mg{\cdot}g^{-1}$로 가장 높았고, 그 다음은 쇠고비의 뿌리>석위 지상부>야산고비 지상부의 순으로 나타났다. 총 플라보노이드 함량은 야산고비의 지상부에서 $27.51mg{\cdot}g^{-1}$로 가장 높았고 야산고비 근경에서 $1.95mg{\cdot}g^{-1}$로 가장 낮았다. DPPH 및 ABTS 라디칼 소거능은 쇠고비의 뿌리 및 근경 추출물에서 가장 높게 조사되었다. 부처손 지하부와 고비 지상부를 제외한 모든 추출물에서 linoleic acid의 산화를 87% 이상 강하게 억제할 수 있는 것으로 확인되었다.

• Natural Product Sciences
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• 제25권2호
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• pp.103-110
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• 2019

We investigated the anti-inflammatory effect of Pyunkang-tang extract (PGT), a complex herbal extract based on traditional Chinese medicine that is used in Korea for controlling diverse pulmonary diseases, on cigarette smoke-induced pulmonary pathology in a rat model of chronic obstructive pulmonary disease (COPD). The constituents of PGT were Lonicerae japonica, Liriope platyphylla, Adenophora triphilla, Xantium strumarinum, Selaginella tamariscina and Rehmannia glutinosa. Rats were exposed by inhalation to a mixture of cigarette smoke extract (CSE) and sulfur dioxide for three weeks to induce COPD-like pulmonary inflammation. PGT was administered orally to rats and pathological changes to the pulmonary system were examined in each group of animals through measurement of tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) and interleukin-6 (IL-6) levels in bronchoalveolar lavage fluid (BALF) at 21 days post-CSE treatment. The effect of PGT on the hypersecretion of pulmonary mucin in rats was assessed by quantification of the amount of mucus secreted and by examining histopathologic changes in tracheal epithelium. Confluent NCI-H292 cells were pretreated with PGT for 30 min and then stimulated with CSE plus PMA (phorbol 12-myristate 13-acetate), for 24 h. The MUC5AC mucin gene expression was measured by RT-PCR. Production of MUC5AC mucin protein was measured by ELISA. The results were as follows: (1) PGT inhibited CSE-induced pulmonary inflammation as shown by decreased TNF-${\alpha}$ and IL-6 levels in BALF; (2) PGT inhibited the hypersecretion of pulmonary mucin and normalized the increased amount of mucosubstances in goblet cells of the CSE-induced COPD rat model; (3) PGT inhibited CSE-induced MUC5AC mucin production and gene expression in vitro in NCI-H292 cells, a human airway epithelial cell line. These results suggest that PGT might regulate the inflammatory aspects of COPD in a rat model.