• 한국육종학회지
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• 제42권5호
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• pp.439-447
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• 2010

Riptortus clavatus, one of the many insects in major crops, damages pods and seeds, which reduces seed vigor and viability in soybeans. This study was conducted to examine the effect of diversely damaged seeds by R. clavatus on seed germination and seedling emergence and to determine the association of damaged seed with quality and yield of soybean sprouts. All seeds damaged by R. clavatus significantly (P<0.05) reduced seed vigor as measured by the rates of seed germination, germination speed, and seedling emergence. Mean seed germination rate of non-damaged seeds in sprout-soybean varieties was 97.8%, whereas the rates of seeds damaged at different levels, 31-50% and 51-80%, were 23.0 and 5.4%, respectively. The rates of seedling rot and abnormal, incomplete germination significantly (P<0.05) increased as the amount of seeds damaged by R. clavatus increased to 5, 10 and 15% against the total seeds for sprout production. Yield of soybean sprouts from seeds damaged at different levels decreased up to 13% as compared to that in normal seeds. In customer preferences on soybean sprout produce, 84% of customers participated in survey preferred to purchase sprouts from seeds with 5% of damaged seeds, but sprouts produced from seeds with 15% of damaged seeds were intended to purchase only by 22% of the customers. Areas of the seed damaged by R. clavatus were readily infected by pathogens as the seed germinated, resulted in deteriorated quality and reduced yield of sprout produce.

• 한국산림과학회지
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• 제83권4호
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• pp.505-511
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• 1994

리기테다 소나무 종자(種子)에 삼투압처리 및 피복(被覆)처리를 하여 균일(均一)한 발아(發芽)와 유묘생육(幼苗生育)을 촉진(促進)하기 위하여 이들 처리(處理) 종자(種子)의 발아(發芽)와 유식물(幼植物) 생육(生育)을 비교(比較) 검토(檢討)하였다. 1. Polyethylene glycol(PEG) 처리농도가 높아질수록 총발아율(TGP)도 높아졌으며 $10^{\circ}C$보다는 $15^{\circ}C$나 $20^{\circ}C$에서 총발아율이 향상되었다. 2. PEG처리기간이 길수록 또한 처리농도가 높을수록 발아속도(GR)가 양호하였으며, 처리농도와 온도간에는 상호 상관관계가 인정되지 않았다. 3. 최대 발아속도(MGR)는 PEG처리기간이 길수록 높았으며, $20^{\circ}C$에서 더욱 뚜렷하게 나타났다. 4. $20^{\circ}C$에서 12일간 PEG 처리시 약 2일 정도의 발아기간(發芽期間) 단축효과가 있었다. 5. PEG 처리는 저수분상태(低水分狀態) 하에서도 발아가 양호하였으며, 피복처리(被覆處理)된 종자는 약해(藥害)로 인한 발아(發芽) 장애현상(障碍現象)이 나타났다. 6. 조파(條播)와 직파(直播)간에는 건물생산량(乾物生產量)에 큰 차이가 있었으며, PEG 처리가 가장 양호한 성적을 보여 주었다. 적당한 토양수분 상태(포장용수량의 약 75%)보다 건조한 토양조건(포장용수량의 약 50%)에서 피복처리효과가 더욱 뚜렷하였다.

• Journal of Plant Biotechnology
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• 제47권3호
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• pp.203-208
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• 2020

The involvement of antifreeze proteins (AFPs; type I and III) in the germination of low temperature-treated petunia seeds (cv. 'Mirage Rose') was investigated. The addition of AFPs (300 or 500 ㎍/l) in low-temperature treatment significantly promoted the germination of seeds compared with that in which AFPs were not added. Among all treatments, treatment with AFP I added at 300 ㎍/l showed the highest germination percentage and improved plant growth. The expression levels of antioxidant-related genes such as superoxide dismutase, peroxidase, and proline synthesis were associated with the germination of low temperature-treated seeds. Overall, this study demonstrated that AFP I may potentially function as a cold-protective agent for the germination of low temperature-treated seeds.

• 한국작물학회지
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• 제41권3호
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• pp.274-285
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• 1996

Po1ymer를 피복한 벼 종자의 종자세 향상과 환경 적응성을 구명하기 위하여 10종의 Polymer를 피복하여 수분흡수력, 호흡량, 발아력 및 포장 출아율 등을 조사하였던 바 그 결과를 요약하면 다음과 같다. 1. Polymer피복 종자의 수분흡수율은 포화습도에서 klucel이 가장 높았고 PVP가 가장 낮았으며, 물에 침청할 때는 klucel이 가장 높았고 maltrin이 가장 낮았다. 2. Polymer 피복 종자의 호흡량은 화영벼 와 일품벼 에서 polymer 피복 종자가 낮았으나, 대청벼와 진미벼는 오히려 높았는데 그 정도는 sepiret 피복 종자가 PVP 피복 종자보다 높았다. 3. Polymer피복 과정 중 기계에 의한 종자피해 정도는 발아율이 1% 내외로 저하되어 극히 미미 하였다. 4. 종자세가 양호한 종자에서는 polymer 피복에의한 발아율의 향상은 없었으나 대부분이 95% 이상의 발아율을 보였고 waterlock, surelease 45 및 sepiret등이 특정 품종에서 발아율이 극히 저조하였다. 5. 발아세는 sepiret 피복으로 향상되었고, PVP 피복으로는 약간 저하하였다. 6. Polymer피복 후 노후화 처리 종자의 발아율은 대부분의 polymer에서 피복 종자가 무처리 종자에 비하여 약간 높았으나 sepiret과klucel피복한 종자의 발아율은 현저하게 저하하였다. 7. 저온 발아성은 daran 8600, maltrin, sacrust 및 opadry 등에서는 향상되었고 나머지 polymer는 저해하였는데, 특히 PVP의 발아율이 가장 낮았다. 8. 초장은 대부분의 polymer 피복 종자에서 컸으나 watelock, PVP, maltrin등의 Polymer에서는 작았다. 저온 발아시 초장은 polymer피복으로 오히려 단축되었다. 9. 포장 출아율은 피복 종자가 훨씬 높았으며 출아 소요시간도 훨씬 단축되었다. 포장수분함량 90 %에서 출아율이 가장 양호하였고 출아 소시간도 단축되었으며, 95년 종자가 94년 종자보다도 출아률이 높았고 출아시간도 단축되었다. PVP가 sepiret보다 더 양호한 출아율을 나타냈다.

• 한국약용작물학회지
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• 제27권6호
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• pp.390-396
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• 2019

Background: Usually ginseng seeds are sown during autumn and spring. Sowing in spring often results in poorer seedling establishment than in autumn. One of the reasons for poor germination could be cold-treatment condition for breakage of physiological dormancy during winter. Here we tested the effects of storage temperature used during cold treatment on germination. Methods and Results: Germination properties were observed after dehiscent seeds were stored as wet and dry at 2℃, -2℃, -3.5℃ and alternating temperature (AT). Seed dryness and storage temperature affected germination properties (p < 0.01). Wet and AT condition germinated highest, and wet and -3.5℃ condition germinated lowest, which was 91.2% and 1.4% respectively. Mean germination time (MGT) of the wet and AT condition was faster than other treatments at 2.4 days, and the dry and -2℃ condition was the longest. Germination performance index (GPI) was highest for wet and AT condition (37.7%) and the lowest for wet and -3.5℃ condition (0.5%). The growth of above-ground and below-ground were the best for wet and 2℃ condition, and wet seeds showed better growth than dry seeds (p < 0.01). Conclusions: For cold treatment, ginseng seeds may not be stored below -2℃ for successful germination during spring sowing.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2003년도 춘계 학술발표대회
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• pp.103-103
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• 2003

This study was carried out to investigate of the biologically active components in germinating Mung bean(Phaseolus radiata L.) and Buckwheat (Fagopyrum esculentum Moench) seeds. During the initial germination, germination ratio of 24 hours pre-soaking Mung bean and Buckwheat seeds were higher about 2∼3% than that of non-soaking. This experiment also was peformed to observe cytotoxic effect of the germinating seeds(germination length : 2, 5, 10mm) extracts against cancer cell lines including human lung carcinoma(Calu-6), human breast adenocarcinoma(MCT-7), human great intestine carcinoma(Caco-2) and human leukemia carcinoma(AML-2/WT). The growth of the cancer cells in medium containing Mung bean and Buckwheat extracts were significantly inhibited degree in proportion to the length of germination seeds, Especially, the results show that a significant shrinkage of Calu-6 cells was observed when the cells were exposed into extract of 10mm germination seeds in germinating Mung bean and Buckwheat seeds.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2018년도 추계학술대회
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• pp.88-88
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• 2018

In order to increase the germination speed and uniformity of tomato seeds, sonication and modified drum priming treatments were investigated to produce high quality seeds for export. Sonication treatment was performed for 5, 10 and 20minutes at an intensity of 5.2, 10.4 and 15.7kHz in water at $15^{\circ}C$. After sonication treatment, seeds were primed with water or 100mM $KNO_3$ for 4days. 40, 50 and 60% seed moisture content (SMC) of hydrated seeds were incubated for 60, 72 and 84h in a container with a relative humidity of 99% at 26rpm for a modified drum priming treatments. Germination speed were highly improved by sonication with osmotic priming. The seed treatment of osmotic priming or hydro priming after sonication or sonication without priming enhanced germination percentage (GP) on the $2^{rd}$day after sowing to 46%, 43% and 28%, respectively, while untreated seeds resulted in only 1% GP. These treatments also highly improved mean germination time (MGT) to 1.4, 1.8 and 2.6days, respectively, when compared to 3.5days MGT of untreated seeds. The modified drum priming treatment (72h incubation after 60% SMC hydration) significantly improved results of 74% GP(on the $3^{rd}$day after sowing), 2.6days MGT and $39%{\cdot}day^{-1}$ germination rate (GR), however, untreated seeds showed 19% GP, 4.1 MGT, and $25%{\cdot}day^{-1}$ GR. Although osmotic priming after sonication, hydro priming showed similar improved germination characteristics, however, modified drum priming is considered as an industrially promising treatment methods considering the shortening of the treatment period and environment-friendly aspects.

• 한국산림과학회지
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• 제99권2호
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• pp.244-250
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• 2010

The aim of this study was to investigate relationship among seed viability and enzymes activities involved in scavenging reactive oxygen species (ROS), especially, superoxide dismutase (SOD), glutathione reductase (GR), and catalase (CAT). In other respects, osmopriming has been demonstrated to reinvigorate aged seeds. Various viabilities of seeds that were ranged from 80 to 100% of germination rate could be produced using osmopriming and accelerated aging treatments. Priming treatment of Pinus thunbergii seeds for 3 days at $15^{\circ}C$ with a polyethylene glycol solution at -1.2 MPa improved their subsequent germination at $25^{\circ}C$. Accelerated aging (3, 6, 9, and 12 days at $41^{\circ}C$ and 100% relative humidity) decreased seed germination percentage depending on aging treatment duration. Electrolyte conductivities of seeds were measured as assay of membrane integrity. The conductivity from electrolyte leakage of P. thunbergii seed was also correlated with seed germinability. Conductivity for control seeds that had 95% of germination percentage was 3.48 ${\mu}S\;g^{-1}$, but jumped as doubled (7.98 ${\mu}S\;g^{-1}$) in 12-day-aged seed that had 80% of germination percentage. Our results demonstrate that aging of P. thunbergii seeds is associated with changes in the electrolyte leakage, lipid peroxidation, and antioxidant defense system. Priming of aged seeds progressively restored the initial germinative ability and resulted in a marked decrease in the levels of MDA and conductivity of seed leachate. These effects of priming were also well recovered of GR and CAT activities in aged seed. The improved seed quality by priming treatment appears at least partly attributable to reduced lipid peroxidation, resulting from enhanced antioxidative enzyme activities that are suggesting the antioxidant defense systems play a key role in seed vigor.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.213-213
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• 2017

High quality seed of proso millet that has high germination percentage, germination speed, and uniformity demanded to increases rates of mechanization in cereal crop cultivation. In order to improve germination characteristics, proso millet seeds were treated with red light, ultrasonication and liquid smoke (LS) solution that generated from hickory wood. All treatments were performed in seed priming solution with 100mM $NH_4NO_3$ at $15^{\circ}C$ for 24hrs under aeration condition. Seeds were exposed under light intensity of 2000 lux for 15m, 30m, 60m, and 120m in priming solution. Ultrasonic treatment was performed at 60%, 80%, and 100% intensity of 21.6 KHz for 5m, 10m, and 20m in priming solution. For LS treatment seed were soaked in 0%, 0.5%, 1.0%, 5.0% and 10.0% of diluted solution with $dH_2O$ or 100mM $NH_4NO_3$ solution. The effect of seed treatment was evaluated with germination percentage (GP), mean germination time (MGT), germination index (GI), germination rate (GR), Germination uniformity (GU) and heath seed percentage (HS). Our results demonstrate that red light (15min) or ultrasonication (21.6kHz, 5min) treatment improved MGT, GI, GR, and GU comparing to untreated control. Importantly, we show that LS treatments have significant effect on the health seedling and germination characteristics. Proso millet seeds that treated with 5% LS solution for 24hrs improves HS up to 97% that similar results obtained in 100mM $NH_4NO_3$ priming for 24hrs. The combined treatment with LS solution and 100mM $NH_4NO_3$ priming were not effective in all treatments. Our results demonstrate that treating seeds with LS or 100mM $NH_4NO_3$ priming or ultrasonication improves germination characteristics. The methods described here will help advance research using this species by increasing the germination performance at which successive seed pellet process.

• 아시안잔디학회지
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• 제1권1호
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• pp.30-36
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• 1987

In order to promote the germination of Korean lawn grass(Zoysia japonica Steud.) seeds a gravimetric seed selection with a mixed chemical solution and the germination promotion with KOH treatment were studied and following results were obtained. 1. When immersed into methanol(S.G., 0.7) 14 percent of immature seeds were precipitated. 2. With water (S.G., 1.0) 36.5 percent of mature seeds were submerged. 3. On a mixture of carbon tetrachloride (S.G., 1.6) and ethylether (S.G., 0.7) in a ratio of 4:6 the seed selection was most effective. 4. When treated with KOH solution the germination rate was 71.01 percent in contrast to 6.0 percent of germination without the treatment. 5. As for concentration of KQH solution, 15 percent depressed the germination (46.7 %) whereas above 20 % the germination rate was gradually increased (79.32 %) up to 40 percent of concentration where the concentration started to depress germination ratio (77.32 %). 6. Finally, in connection with the duration of KOH treatment, a time lapse of up to 20 minutes depressed the germinating ratio(70.02 %) and 30 minutes to 45 minutes increased this ratio to 94.52 percent. 60 minutes of treatment depressed the germination to 75.62 percent.