• 한국연초학회지
• /
• 제18권2호
• /
• pp.126-131
• /
• 1996

In this study effects of isolation distance, transplanting time of maternal plants, and bagging of flower head with the gauze-cloth bag on the outcrossing of burley tobacco (Nicotiana tabacum L.) were investigated. Also the effect of fertilizer level and control of the number of capsules per plant on seed production and quality were examined. A male sterile line. produced 0.3 to 3.8 capsules Per plant when it was Planted with normally flowering tobacco with the average outcrossing of 7.2 plants, ranging from 2 to 18 out of 20 plants. With the farther the isolation distance between maternal plants and pollen donor plant, the lower the outcrossing occurred. Outcrossing occurred even at the isolation distance of 312 m. When the maternal plants were transplanted 35 days after transplanting the pollen donor ones, the outcrossed plants were not decreased significantly. The bagging of the flower head with the gauze-cloth bas (#0.9∼ 1.0 mm) decreased the outcrossed plants significantly, but couldn't prevent the outcrossing completely. The seed amount per plant was higher in the highly fertilized cultivation. The number of seed capsules per plant affected significantly on seed yield and quality. When the seed capsules was controlled by 30 or 50 capsules per plant, the weight of 1,000 seeds and germination rate were higher than those with 70 or 90 capsules per plant. Key words : Nicotiana tabacum, outcrossing, bagging.

• BMB Reports
• /
• 제29권6호
• /
• pp.540-544
• /
• 1996

Ferritins from germinated pumpkin seeds were isolated by ammonium sulfate precipitation (0.55 saturation), ion-exchange chromatography on DEAE-cellulose, and gel filtration chromatographies on Sephacryl S-300 and Sephadex G-100. Pumpkin ferritin contains less iron than soybean ferritin. Pumpkin ferritin cross-reacted with anti-soybean ferritin antiserum made in rabbit, and showed two distinct antibody reactive bands, both of equal intensity. The pumpkin ferritins corresponding to the two bands were separable by centrifugation in a sucrose gradient (20~50%). The molecular weights of the native pumpkin ferritins based on the estimation of sucrose gradient centrifugation, gel filtration on Sephacryl S-300 and non-denaturing polyacrylamide gel electrophoresis appeared to be: 530~580 KD (the large molecular weight pumpkin ferritin) and 330-360 KD (the small molecular weight pumpkin ferritin) The large molecular weight pumpkin ferritin contains less iron. Both pumpkin ferritins cross-reacted with anti-soybean ferritin antibody with a spur formation suggesting partial antigenic recognition.

• 한국자원식물학회지
• /
• 제28권6호
• /
• pp.710-717
• /
• 2015

Humankind has been searching for medicinal materials from various plant sources in an attempt to treat disease. Mistletoe is one indubitable plant source for these materials due to its effectiveness in treating various diseases, but it has almost disappeared from the mountainous areas of Korea due to excessive harvesting. In this study, in order to select host tree species for Korean mistletoe [Viscum album var. coloratum (Kom.) Ohwi] by seed inoculation and to clarify the effect of host specificity among various tree species were conducted for the purpose of gaining basic information for the artificial cultivation of Korean mistletoe. Almost all the seeds of Korean mistletoe germinated in vitro at the temperature of 15℃. Among host trees used in this study, Prunus mume showed the highest parasitic affinity with inoculated Korean mistletoe, compared with any other host plants. However, treatment of hormones could not increase the low survival rate of Korean mistletoe on the host trees.

• Mycobiology
• /
• 제32권4호
• /
• pp.149-154
• /
• 2004

Toxin produced by Colletotrichum falcatum Went, the incitant of red rot of sugarcane was isolated, purified and assayed to determine host specificity and identify its chemical nature. The toxin was found to be not host specific as it inhibited germination of various seeds(gram, greengram, blackgram, pea, cowpea, rice and sugarcane) as well as different seedlings viz. tomato, coriander, pea and rice. The toxin consists of two distinct fraction-one fraction having $R_f$, value at 0.36 producing identical red rot lesion when inoculated at leaf midrib of sugarcane, and the other having $R_f$, value at 0.72 not showing any red rot lesion. Chromatogram of high performance liquid chromatography(HPLC) of the red rot lesion causing fraction showed a sharp peak at 1.62 min of retention time(RT), and spectral analysis indicated the presence of following chemical $CH_3$ - groups-C-H, C=O, C-N, $-CH_3,\;-CH_2$ -CH and molecular mass of the compound was 203. - ($M^+,\;C_{11}H_{11}N_2O_2$).

• 한국유기농업학회지
• /
• 제19권spc호
• /
• pp.230-233
• /
• 2011

We collected and examined germplasm resources of lettuces, edible chrysanthemums and mallows for organic farmers. We found that 3 genotypes (OL-51, OL-52, and OL-63) are suitable for organic cultivation. The main use of treatment for grey mold among eco-friendly pest control methods is helpful to increase efficiency of seed-gathering after collecting and evaluating a total of 64 kinds of lettuces-collected 43 South Korea local varieties, 8 kinds cultivated by the Highland Agriculture Research Center, 4 kinds of cultivated lettuces, and 9 kinds for sales- to choose appropriate kinds of lettuces for organic cultivation in South Korea. We chose those with more than 300 leaves (OC-18, OC-44, and OC-51) after evaluation of yield characteristics of a total of 62 edible chrysanthemums. We covered each genotype of seed of edible chrysanthemums when gathering the seeds by ourselves so as not to decrease the purity of each genotype. We chosed OM-38, OM-39, and OM-40 for their yield performance and germination rates among the total 41 genotypes of mallows. We selected 9 genotypes of lettuces, editable chrysanthemums and mallows with possibility of organic cultivation and found that they can be used permanently through self seed-gathering on organic farms.

• Current Research on Agriculture and Life Sciences
• /
• 제20권
• /
• pp.25-31
• /
• 2002

1. 꽃무릇으로부터 용매를 다르게 하여 잎과 인경의 MeOH와 수용추출물을 bioassay 한 결과, 농도가 5% 및 10%로 증가하였을 때는 전혀 발아되지 않았으며, 1%에서는 근장이 크게 억제되었다. 용매간의 추출효과에는 차이가 없었으나, 상추의 생장에서는 인경추출액이 잎추출액보다 억제효과가 다소 높은 것으로 보였다. 2. 꽃무릇의 인경을 추출하여 UV를 조사하였을 때 Rf 0.49-0.52에서 보인 spot는 GC-MS에 의해 lycoris alkaloid계통의 lycorenan으로 동정되었다.

• Journal of Plant Biotechnology
• /
• 제50권
• /
• pp.108-114
• /
• 2023

In vitro conservation is one of the most effective strategies for rare plant protection, especially for orchid species. To maximize the success rates of in vitro explant establishment (stage I) in conservation programs, the application of tissue culture additives such as Plant Preservative Mixture^TM (PPM^TM) should be emphasized. In this study, we used Dendrobium thyrsiflorum Rchb.f. (1875) seeds and seedlings as a model for the evaluation of PPM^TM's phytotoxicity in the meristematic tissues of epiphytic orchids. PPM^TM had no observable inhibitory effect on protocorm, shoot, or root development when it was supplemented at 0.1%. PPM^TM supplementation caused adverse effects on D. thyrsiflorum explants at concentrations > 0.2%. At high concentrations, young in vitro seedlings showed damage, especially at the root tissue level. Based on this model, supplementation of 0.1-0.2% PPM^TM to culture media was successfully implemented to establish in vitro cultures of other rare orchid species in our conservation program.

• 한국식품저장유통학회지
• /
• 제16권6호
• /
• pp.971-976
• /
• 2009

건강 웰빙식품의 하나로서 수요가 증가하고 있는 새싹채소류는 미생물학적 관점에서 생산단계별 위해분석과 안전성 기준이 부재한 실정이다. 본 연구에서는 생산현장에서 새싹채소의 재배공정별 미생물학적 오염도를 분석 평가하였다. 새싹채소 종자의 일반세균수 및 대장균군수는 각각 3.04~5.21 log CFU/g, 1.80~3.86 log CFU/g의 범위로 나타났으며, 소독유무에 관계없이 재배기간 동안 일반세균(6.99~8.26 log CFU/g) 및 대장균군수(3.70~7.15 log CFU/g)는 증가하였고, 최종 새싹채소에서도 높은 수치를 나타내었다. 소독된 종자의 발아 후 전 단계에서는 식중독균이 검출되지 않았으나, 종자소독을 하지 않은 국내산 무와 유채에서 E. coli가 검출되었고, 국내산 적무에서 E. coli와 식중독균인 Bacillus cereus 및 Listeria monocytogenes가 검출되었다. 따라서 종자생산에서부터 새싹채소의 생산 전 단계에 걸친 HACCP 가이드라인을 마련하여 중점적으로 관리하는 것이 필요하다.

• 한국작물학회지
• /
• 제45권3호
• /
• pp.195-198
• /
• 2000

무경운 담수표면산파재배시 잡초성벼의 발생양상과 비선택성 및 토양처리제를 이용한 잡초성벼 방제 결과는 아래와 같다. 1. 무경운에 의해 토양표면에서 월동한 잡초성벼의 생존율은 92.7%였고 재배벼는 4.3%였다. 2.토양내의 잡초성벼 발생심도는 건답에서 6.8cm, 무논에서 3.0cm, 그리고 무경운답에서 1.5cm로, 무경운답의 잡초성벼 발생은 건답과 무논에 비하여 토양 표층부위에서 주로 발생하였다. 3. 4월 하순의 우리나라 중부지역 평균기온인 13$^{\circ}C$에서 잡초성벼 발아에 소요되는 일수는 14-15일이었다. 4. 무경운상태에서 포장 표면위에 떨어져 있는 잡초성벼의 발아를 유도한 후 비선택성 제초제인 paraquat 처리한 결과 92.2%의 잡초성벼를 방제할 수 있었으며, 토양처리제 중 가장 높은 출아억제를 보인 제초제는 oxadiazon으로 53.3%의 방제 효과가 있었다. 5. Paraquat과 oxadiazon의 체계처리에 의해 96.4%의 잡초성벼를 방제할 수 있었다.

• 한국약용작물학회지
• /
• 제6권2호
• /
• pp.102-107
• /
• 1998

종자번식을 통한 다량육묘로 다량재배의 필요성이 제기되고 있는 둥굴레는 발아 과정이 복잡하기 때문에 다량육묘 또는 이에 관한 연구를 위하여는 발아 과정을 체계적으로 자료화하여야 할 것이다. 본 연구는 둥굴레의 종자 구조와 유묘 출현 특성을 체계화하여 종자를 이용한 다량번식 체계에 대한 정보를 얻고자 수행 되었다. 둥굴레 종자는 직생배주(直生胚珠)로서 경실(硬實)이었으며, 배는 linear형으로 종자의 중앙에 배열하고 있었다. 발아는 정단조직에 소주아(小珠芽)와 뿌리의 시원체(始原體)를 가지고 있는 배의 아래 부분이 신장하여 돌출하는 형태로 진행되었고, 배유의 저장 양분은 생장하고 있는 배가 배유를 잠식하는 방법으로 이용되고 있었다. 배가 돌출한 후 시간이 경과하면서 소주아(小珠芽)와 뿌리가 발달하고 형성된 소주아(小珠芽)의 정단에 상배축이 돌기하였다. 상배축(上胚軸)은 다량육묘에서 저온처리를 통한 휴면타파 처리 후에야 신장하기 시작하였으며, 출아 후 형성되는 단자엽과 초장은 출아 중의 광도에 크게 영향을 받았다. 한편 재배 또는 가공에 이용되는 지하경은 소주아(小珠芽)가 최초의 조직으로 소주아(小珠芽)에 형성된 마디로부터 다음의 지하경이 분지되는 형태를 보였다.