• The Korean Journal of Malacology
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• v.32 no.2
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• pp.67-71
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• 2016

In order to obtain a large number of fertilized eggs for seedling production, experiment was carried out examine effects of serotonin on spawning of the Pacific oyster Crassostrea gigas. The shorter response time to initial spawning in case of serotonin injection showed, the higher serotonin injected with 7.6-27 min. The response time to initial sperm releasing showed the same tendency with female. The highest response rates and eggs amount spawned were showed in the highest concentration. The serotonin injection had no effect on frequency of germinal vesicle breakdown (GVBD), fertilization and hatching rate.

• Korean Journal of Weed Science
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• v.12 no.3
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• pp.292-308
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• 1992

Recently agricultural situations are being placed in unfavourable socio-economic environment as followed by rapid decrease of rural population, poor labor quality and high wages of rural society due to high speedy industrialization of social structure in Korea. In addition to those circumstances, under the UR system to be expected in the early future, free trade of agricultural products will be faced inevitably in Korea. Practically prices of rice as a principle food in this country are expensive about three times compared to those of foreign rice markets, and so how to increase the international competitiveness and food supplies are important problems to be solved rapidly. Accordingly an urgent goal is reduction of agricultural production cost by the improvement of labour productivity as a labour saving and cost down cultivation methods as the direct seeding cultivation in rice. But there are many problems in the direct seeded rice cultivation. The important things to be improved in that cultivation are development of rice varieties with lodging tolerance, effective seedling stand, weed control, irrigation management and fertilizing and so on. Moreover agricultural basis as irrigation facilities, land consolidation and farm machineries must be improved for the stability of rice cultivation in the future.

• The Korean Journal of Malacology
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• v.21 no.1
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• pp.65-70
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• 2005

The purpose of this study is to determine the appropriate substrate for larval settlement and spat growth in the purple clam, Saxidomus purpuratus in laboratory culture. Larvae were reared with 3 different types of sediments (mud, sand, and mixed) for 46 days in settlement experiment, and settled spats were further grown in 3 types of sediments for 36 weeks in growth experiment. The density of settled spats in muddy sediments was more than 2 times higher than those in mixed or sandy sediments. But, the average size of settled spats in muddy sediments was smaller than those in mixed or sandy sediments. After 36 weeks of growth period, growth rate decreased as shell length increased. When shell length was less than 2 mm, growth rate in mixed sediments was significantly higher than that in sandy sediments. When shell length was more than 2 mm, there was no significant difference in growth rate among different substrates. Sediment type affected growth rate only when the spats were relatively small (less than 2 mm). Muddy sediments seems better for larval settlement, while mixed sediments is best for spat growth. We suggest the laboratory procedure for enhancing seedling production of S. purpuratus.

• Journal of Aquaculture
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• v.5 no.1
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• pp.81-91
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• 1992

The cultivation of marine microalgae as a live feed is essential for the seedling production of marine animals. It has some technical problems. Of these, the isolation and maintenance of the pure strains of microalgae from the nature are difficult for general mariculturists and researchers. To meet these problems, it needs to establish the culture collection of the microalgae in order to supply the strains to the demanders. In this research, 80 strains of microalgae were isolated from the coastal water of Korea by the methods of capillary pipette, plating on agar and dillution. A culture collection was established in the Department of Aquaculture, National Fisheries University of Pusan. The 117 strains of the microalgae maintained in the culture collection will be supplied to the demanders without any difficulties.

• Journal of the Korean Institute of Landscape Architecture
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• v.15 no.1
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• pp.9-16
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• 1987

In Seoul metropolitan area, rockily denuded fores lands are widely distributed. Out of the rock-exposed hillslopes, Kwanak-Samsung mountains and Bookhan-Dobong mountains areas are the most severely eroded land at present To develope the rehabilitation measures for the rock-exposed hillslopes, it is essentially required that mechanisms of rock debris production and movement from the rock-exposed hillslopes should be studied. And also, suitable experiments for planting techniques should be practised at the field level. In this context, some experiments for the on-site fixation of the unstable stone-debris including plantation establishments on the rock-exposed hillslopes, have been carried out for 4 years from 1983 to 1986 at Kwanak mountain hillslopes. The results may be summarized as follows : 1) The soil environment-tolerable tree species for plantation extablishment on rock-exposed hillslopes include Buxus microphylla var. koreana, Forsythia koreana, Juniperus chinensis var. sargentii, Alnus hirsuta and Pinus rigida. These trees have, however, been showed fairly good growth performence at the places having more than about 30cm of soul layer depth above rock-bed. 2) In raising of seedlings to plant on the rock-exposed hillslopes, containerized seedling practices knave been appeared as the most adoptable measures. 3) In fixation of the unstable stole debris-and-sands on the on-site of rock. exposed hillslopes, the stone-lined barrier measures are more effective at comparatively large-sized places of rock-exposed hillslopes while the stone-bowed barrier measures are more effective at small-sifted isolated sold-debris places. The stone-buttressed terraces measures are more effective at the soil-composed hillslopes rather than rock-exposed hillslopes.

• The Korean Journal of Malacology
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• v.15 no.2
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• pp.115-119
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• 1999

We investigated the processes of egg and larval developments for aquaculture technique development of seedling production fo the flat oyster, Ostrea denselamellosa. Teo flat oyster of larviparous type was different from the pacific oyser (ovivarous type) because their larvae (trochophore and prodissoconch larvae) in the gill released into the seawater. The process of egg development was observed by artificial fertilization at $25^{\circ}C$, using a dissecting method. The sizes of Unfertilized eggs ranged from 80 to 90 $\mu\textrm{m}$ and fertilized eggs with globule-shape was 90-100 $\mu\textrm{m}$. The Polar body appeared after fertilization and egg cleavage began within 1 hour, reaching the blastula stage after 10 hours. The trochophore in the gill appeared 2-3 days after fertilization and grew to the prodissoconch larvae (130 140 $\mu\textrm{m}$) having a complete shell after 1-2 days. The shell of prodissoconch larvae grew to 205 220 $\mu\textrm{m}$ after 10 hours, and then they became umbo stage larvae showing oval in shape. The velum of umbo stage larvae was degenerated about 17-20 days after fertilization and grew into a pediveliger with a developed foot, at this time, the shell length size was 320 360 $\mu\textrm{m}$.

• Journal of the Korean Society of Tobacco Science
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• v.14 no.1
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• pp.3-11
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• 1992

The present study examined various environmental and cultural media conditions for in vitro "rescue" of cross-fertilized ovules formed through sexual crosses between Nicotiana rustica and N. tabacum cv. BY4. The response ovules to two cultural procedures was compared; ovules were cultured either separately or left attached to the placenta. Total yield of seedlings and percent of normal seedlings were increased by culturing individual ovules separately, rather than on excised placenta. Total yield of seedlings and number of normal seedlings were produced following in vitro culture of individual fertilized ovules of N. rustica X M tabacum cv. BY4 at four days post-pollination on NN medium containing 2% sucrose. In the in vitro culture of fertilized ovules, high sucrose concentration increased the frequency of seedlings of abnormal appearance. Therefore, sucrose should be supplied to developing ovules at gradually decreased concentrations. Culture of fertilized ovules from three to eight days after pollination gave increased number of seedlings, but with delayed cultral time the number of morphologically normal seedling were decreased. Hybrids were uniform in appearance and showed vegetative heterosis but flower characteristics were generally intermediate between those of the parents. All hvbrids evaluated were self-sterile.f-sterile.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.45 no.1
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• pp.55-58
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• 2000

Germination of sweet and super sweet corn is lower than normal corn due to the higher sugar and lower starch contents of kernels. Sweet corn seeds are easily deteriorated in the field under the unfavorable condition, therefore it is important to identify the optimal harvesting time for seed production. This trial was conducted to investigate the responses of germination percentage of shrunken-2(sh2), brittle(bt), sugary(su), and sugary enhancer(se) hybrids in relation to harvesting dates. Eight hybrids of four different gene sweet corns were harvested at 15, 20, 25, 30, 35, 40, 45, and 50 days after silking(DAS). Germination test was performed using paper towel method. Mean germination percentages across eight hybrids showed the highest value at 45 DAS. There were significant differences among genes and within gene for germination. Shrunken-2 hybrid Mecca was higher than su hybrids for germination, indicating that sh2 would not be poorer than su Late harvesting beyond the optimal harvesting date might not be desirable because of more lodging and ear rots. Theoretical optimal harvesting date estimated from the regression equation was 40.9 DAS, however, practical date for harvesting would be a few days later than the estimated date if seedling vigor might be considered. Kernel dry weight per ear showed similar response to germination. Regression equation showed the highest kernel dry weight at 40.7 DAS. Significant correlations between kernel dry weight and germination were observed, impling that kernel dry matter accumulation would be an important factor for germination.

• Current Research on Agriculture and Life Sciences
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• v.21
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• pp.11-16
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• 2003

Lines bred for resistance to Phytophthora capsici by incorporation of resistance to P. capsici in PI201234 or CM334 into 'Subi' and 'Chilseong', land races in Youngyang, and 'Punggak', a land race in Cheongdo in Gyeungbuk province, and lines bred for fortification of one of them above with resistance to viral complex, and tolerant selections from another landrace collection from Punggak (KC268) were evaluated for resistance to P. capsici by inoculation at seedling stage. Almost all the breeding lines showed high level of resistance to P. capsid and selections from KC268 showed tolerance or moderate resistance to P. capsid. The selected plants were grown in a net cage in an outdoor nursery for seed production. Utilization of the lines in breeding was discussed.

• Journal of Aquaculture
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• v.10 no.1
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• pp.17-24
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• 1997

In order to understand the spawning and larval development of Perinereis aibuhitensis for the effective seedling production, the influence of water temperature on spawning induction, egg and larval development of the worm were investigated from October 30, 1989 to October 30, 1990. Main spawning period was from July through September, and average diameter of mature eggs was $220\mu m$. The relationship between the adult weight (Wt) and the number of spawned eggs (NS) was given as follws : NS=48635.589Wt1.3044 (r=0.8572). Adult males and females died immediately after spawning. Trochophore larvae developed 12 hours after fertilization, and hatched out after 56 hours.