• Korean Journal of Medicinal Crop Science
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• v.2 no.2
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• pp.105-109
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• 1994

This experiment was conducted to know the labor saving effect and reducing production cost by agricultural mechanization in the cultivation of Bupleurum falcatum. Labor reducing effects of the drilling seeder by hand and the machine attached to two wheel tiller were 97%, but emergency rate was highest in the former. Dry root yield per plant was increased by low amount of seed sowing but that yield per unit area was increased at much seeding amount in the seeder attached to the tiller. The drilling seeder by hand was showed highest standing ratio of seedling and produced yield to 84.1kg of root yield per 10a. Labor saving effect was the best at the multipurposes mechanized harvester and labor saving and famer's income ratio were increased to 69% and 50% respectively. Labor time and cost were reduced to 74% and 69% respectively by mechanization of sowing and harvest cultivation practice on Bupleurum falcatum.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.17
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• pp.7749-7754
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• 2015

Background: Currently, cationic liposome has become the commonly used vehicles for gene transfection. Furthermore, one of the most significant steps in microRNAs expression studies is transferring microRNAs into cell cultures successfully. In this study we aim to approach the feasibility of transfection of cervical cancer cell lines mediated by liposome and to obtain the optimized transfection condition for cervical cancer cell lines. Materials and Methods: $Lipofectamine^{TM}2000$ as the carrier, miR-101 mimic was transfected into Hela cells and Siha cells. Using green fluorescent protein as reporter gene, to set different groups according to cell seeding density, the amount of miRNA, miRNA and the proportion of Liposomes, Whether to add serum into medium to study their impact on the liposomal transfection efficiency. Finally, MTT assay was used to analyze the relative minimal cell toxicity of liposome reagents. Results: The seeding density of Hela cell line and Siha are $1.5{\times}10^4$ (per well of 24 well plates), miRNA amount is 1ul of both, the ratio of miRNA and liposome is 1:0.5 of Hela cell line; 1:0.7 of Siha cell line respectively, after 24 hours we can get the highest transfection efficiency. Compared with serum medium, only Siha cells cultured with serum-free medium obtained higher transfection efficiency before transfection (P<0.01). MTT assay showed that according to the above conditions which has the lowest cytotoxicity. Conclusions: The method of Liposome to transfected is a suitable way and it can be an efficient reagent for miRNA delivery for Hela cells and Siha cells in vitro. It may serve as a reference for the further research or application.

• Korean Journal of Agricultural Science
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• v.14 no.1
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• pp.1-15
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• 1987

This experiment was conducted to investigate the effect of nitrogen top-dressing and Jangyeopkong was planted under two different seeding time (single cropping-May 15, after barley cropping-June 18), four levels of nitrogen top-dressing (0, 3, 6, 9 kg/10a) and two times of nitrogen top-dressing (Hilling time, Flowering Time). The results obtained are summarized as follow: 1. The days to flowering and maturity were delayed a day longer in hilling times than flowering times of nitrogen top-dressing. 2. The number of nodes of main stem and length of internodes didn't show significance among treatments. But, the number of nodes of branches was much higher when the soybean was planted on May 15, and were higher hilling time than flowering time as the amount of nitrogen top-dressing increased. Especially, the number of nodes of branches was high when 6kg of nitrogen was applied during hilling time. 3. The fresh and dry weight of stem and leaves at 10, 25 and 40 days after flowering were increased by increasing the amount of nitrogen top-dressing. More apparent effect of nitrogen was attained high significant when nitrogen was applied at the time of hilling rather than flowering time. 4. The number and fresh weight of nodule, and dry weight were apparently decreased after barley and were decreased according to the increasing the amount of nitrogen top dressing. The degrees of decreasing was more apparent in the hilling time than in the flowering time. 5. The number of pods per plant, and number and weight of grain per plant were higher when the soybean was planted on May 15, the amount of top dressing increased and hilling time rather than flowering time. Especially, yield component were highest when 6kg of nitrogen was applied during hilling time. Also, the grain yield per 10a showed high significance among treatment, and were high when 6kg of nitrogen was applied during hilling time.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.41 no.4
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• pp.482-488
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• 1996

The experiment was carried out to the find variation of the sugar contents in the tip and basal part of the cotyledon and remaining portion of embryos in Phaseolus vulgahs seeds during germination with HPLC analysis method. Water content in cotyledon of kidney bean seed was about 6.4~6.5 of fresh weight and incresed to 45.8~71.2% during germination showing that tip part of cotyledon has more water content by 1.1~3.8% compared to the lower part of it. Higher water onten was observed in the rest parts of the seed except cotyledon such as plumule, radicle and hypocotyl showing that it increased to 72.2~93.3% depending on the different tissue organs. Main important sugars in kidney seeds during germination stages are; raffinose, sucrose, glucose and fructose, and the amount are differed with the kinds of embryo in kidney bean seed organs and stages of germination. Raffinose amount in kidney bean is increasing repeatly when seeds become wet but disappear it soon after seed have germinated especially in growing embryo parts. Raffinose in basal parts of cotyledons were still presented some an amount after germination. Sucrose is synthesized highly in plumule embryo at the beginning stage of germination but disappeared it from 5 days after seeding stages. Amount of sucrose in cotyledon of kidney seeds and seedlings increased continuously after germination. The amount of glucose and fructose in the cotyledons of kidney seeds during germination varied 5~10% or 5~15% but in the germinating and growing organs, plumule, they increased continuously after germination.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2009.06a
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• pp.342-343
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• 2009

Paper factories use a large amount of water and same amount of wastewater is generated. It is important to purity and recycle the wastewater because of water shortages and water pollution. The existing water treatment facilities like precipitation process need large-scale equipment and wide space to purity the wastewater of paper factory. High gradient magnetic separation (HGMS) system has the merits to purity rapidly because of large voids at filter and to occupy small space. In this paper, two types of superconducting magnets were used for HGMS systems. Cryo-cooled Bi-2223 superconducting magnet system with 70 mm room temperature bore and 200 mm of height was prepared. Cryo-cooled Nb-Ti superconducting magnet with 100 mm room temperature bore and 600 mm of height was used for magnetic separator. Magnetic filters were designed by the analysis of magnetic field distribution at superconducting magnets. The various magnetic seeding reactions were investigated to increase the reactivity of coagulation. The effects of magnetic separation of wastewater were investigated as variation of magnetic field strength and flow rate of wastewater.

• Journal of the Korean Institute of Landscape Architecture
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• v.27 no.1
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• pp.29-38
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• 1999

Three kinds of soil surface applied herbicides and three kinds of foliage applied herbicides were used to study weed control effect, appropriate concentration, phyto-toxicity against trees and economical efficiency of weed control on trees, Pinus thunbergii, Picea abies, Chamaecyparis obtusa, Quercus accutissima, and Fraxinus rhynchopylla in a nursery. The results were obtained as follows; 1.The control effect of the soil surface applied herbicides showed that the three herbicides; Alachlor, Pendimenthalin and Simazine were alike in their holding effect of weed development, and the sensibility of weed spp. on the herbicides was different. The mean control effect was 58~89%. 2. The weed control effect of the foliage applied herbicides demonstrated that Paraquat dichloride was the best of 84~95% and followed by Glufosnate ammonium and Glyphosate. Especially when the half of its standard amount was treated, the effect of Paraquat dichloride was higher than the other herbicides. 3. Generally, the weed control effect became better as the concentration of the herbcide getting higher. As the concentration of the herbicide got higher, the restraining effect of weed development was continued for a long time in the soil surface applied herbicides, while the weed control effect appeared earlier in the foliage applied herbicides. 4. As the result of the soil surface treatments, 20% of short seedings of Fraxinus rhychopylla treated with twice amount of standard Alachlor died and the rest seeding showed low growth. In the other treatments, there was neither harmful effect of herbicides on the seedings nor growth decrease of them compared to those weeded by men. 5. When treated with foliage applied herbcides, leaves were partially of and discolorated in most treatments. About 0.4~6.2% of Fraxinus thynchopylla, which had short seedings died, and there was no difference in growth with the other treatments. 6. The herbicides showed better economical efficiency over 68% than weed control by men. Especially, Alachlaor of the soil surface applied herbicides showed the highest efficiency(77.6%), while Paraquat dichloride of the foliage applied herbicides was the best(70.3%)..

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2009.05a
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• pp.4-4
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• 2009

Paper factories use a large amount of water and same amount of wastewater is generated. It is important to recycle the wastewater because of water shortages and water pollution. The existing water treatment facilities like precipitation process need large-scale equipment and wide space to purify the wastewater of paper factory. High gradient magnetic separation (HGMS) system has the merits to purify rapidly because of large voids at filter and to occupy small space. In this paper, two types of superconducting magnets were used for HGMS systems. Cryo-cooled Bi-2223 superconducting magnet system with 70 mm room temperature bore and 200 mm of height was prepared. Cryo-cooled Nb-Ti superconducting magnet with 100 mm room temperature bore and 600 mm of height was used for magnetic separator. Magnetic filters were designed by the analysis of magnetic field distribution at superconducting magnets. The various magnetic seeding reactions were investigated to increase the reactivity of coagulation. The effects of magnetic separation of wastewater were investigated as variation of magnetic field strength and flow rate of wastewater.

• Restorative Dentistry and Endodontics
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• v.22 no.2
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• pp.560-574
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• 1997

The present study was undertaken to investigate the crystal growth onto human enamel mineral and synthetic hydroxyapatite(HA) seeds in media resembling the enamel fluid composition. Effects of fluoride at high concentrations on the precipitation were also examined in a bench-top crystal growth model adopting a miniaturized reaction column. The Ca, P and F concentrations and pH values of the inlet and outlet solutions were determined. The recovered solid samples were weighed to assess the amount of minerals precipitated during the experimental period, and finally viewed under a scanning electron microscope. Remarkable findings were that 1) both biological and synthetic seeds with the same total surface areas yielded similar amounts of crystal growth, 2) the amount of crystal growth was accelerated in a manner depending on fluoride concentrations in the media, 3) SEM observations disclosed that without the addition of fluoride, precipitation of thin, plate-like OCP crystals became prominent, but by increasing the fluoride concentration(beyond 1ppm F), rod-like crystals having a pointed edge were most frequently observed, without any evidence for precipitation of the plate-like crystals. Furthermore, the dimension of rod-like crystals was increased in proportion to fluoride concentrations, 4) there was no difference in the morphological feature of precipitated mineral phase upon seeding between human enamel seed and synthetic HA seed. The overall results support the view that the seeded crystal growth model is of value to gain insight into the mechanism of enamel crystal growth under fluoride regimens.

• Journal of Animal Science and Technology
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• v.63 no.2
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• pp.339-353
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• 2021

This study aimed to assess the causality of different climate variables on the production of whole crop maize (Zea mays L.; WCM) in the central inland region of the Korea. Furthermore, the effect of these climate variables was also determined by looking at direct and indirect pathways during the stages before and after silking. The WCM metadata (n = 640) were collected from the Rural Development Administration's reports of new variety adaptability from 1985-2011 (27 years). The climate data was collected based on year and location from the Korean Meteorology Administration's weather information system. Causality, in this study, was defined by various cause-and-effect relationships between climatic factors, such as temperature, rainfall amount, sunshine duration, wind speed and relative humidity in the seeding to silking stage and the silking to harvesting stage. All climate variables except wind speed were different before and after the silking stage, which indicates the silking occurred during the period when the Korean season changed from spring to summer. Therefore, the structure of causality was constructed by taking account of the climate variables that were divided by the silking stage. In particular, the indirect effect of rainfall through the appropriate temperature range was different before and after the silking stage. The damage caused by heat-humidity was having effect before the silking stage while the damage caused by night-heat was not affecting WCM production. There was a large variation in soil surface temperature and rainfall before and after the silking stage. Over 350 mm of rainfall affected dry matter yield (DMY) when soil surface temperatures were less than 22℃ before the silking stage. Over 900 mm of rainfall also affected DMY when soil surface temperatures were over 27℃ after the silking stage. For the longitudinal effects of soil surface temperature and rainfall amount, less than 22℃ soil surface temperature and over 300 mm of rainfall before the silking stage affected yield through over 26℃ soil surface temperature and less than 900 mm rainfall after the silking stage, respectively.

• Journal of Environmental Health Sciences
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• v.28 no.5
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• pp.22-27
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• 2002

The composting practice has been recognized as the most popular way of controlling food waste and many attempt have been made in the field to establish more efficient and economical process. Some of the efforts are mixing cured compost with sawdust as alternative bulking agent, seeding commercially produced microorganism and/or combination of above. However, verification of such efforts is often restricted because of either the lack of engineering consideration on the limitation of composting facility scales. In this study, the effect of mixing materials in food waste composting was investigated by controlling the combination and the mixing ratio of them. When the cured compost was mixed with saw dust. the decomposition of organic material was proven to be more active by observing the compost temperature, the oxygen (O$_2$) consumption, and the cumulative carbon dioxide ($CO_2$) profile. However, the quantity of compost mix-ing seemed not to influence the reaction as long as the minimum required amount was mixed. The feeding of com-mercially produced microorganism had a tendency to prolong the thermophilic stage, which helped to increase the decomposition but it resulted in composting period. Regardless of the composting condition, bacteria and actinomycetes increased in population as the reaction approached to the end. The population of bacteria and actinomycetes were rel-atively higher than those of fungi and yeast throughout the reaction.