• The Korea Journal of Herbology
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• v.34 no.3
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• pp.19-30
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• 2019

Objectives : This study is to propose the identification keys based on stereoscopic examination of 8 seed herbs in 2 categories (Phyllolobobii Semen (PS) with 4 Astragali Semen (AS), and 2 Lepidii seu Descurainiae Semen (LDS) with 1 Drabae Semen (DS)) which have difficulties in discrimination with visual observation. Methods : We reviewed the description of original plants and their medicinal parts from the literature. The original plants were collected, identified, confirmed as specimens, and compared to the samples distributed in the market. The first identification was made by visual observation, and insufficient points were supplemented by stereoscopic observation. Identification criteria were set by considering morphological characteristics of authentic herbs, percentage of adulterants, and distinction between authentic herbs and adulterants. Results : The original plants of PS and AS could be distinguished by upright or lying form of stem, color of flowers, number of leaflets, and presence of hair of fruits. LDS and DS could be distinguished by leaf arrangement on stem: radical or cauline, whole plants size, leaf division, color of flowers, and shape of fruits. The herbal medicines of PS and AS could be distinguished by seed surface pattern, size, and hardness. LDS and DS could be distinguished by size, shape, viscosity when chewed, and degree of mucous layer formation when soaked in water. Conclusions : This study suggests the identification keys of original plants and herbal medicines. Especially, since fine seed herbs are difficult to distinguish by visual observation, the stereoscope should be applied to the discrimination.

• Journal of the Korean Applied Science and Technology
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• v.21 no.4
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• pp.370-379
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• 2004

Highly crosslinked micron-size monodispersed PMMA/PDVB and PS/PDVB particles were prepared using seeded multi-stage emulsion polymerization. PMMA and PS seed particles were synthesized by seeded multi-stage emulsion polymerization and soap-free emulsion polymerization. Then PMMA/PDVB and PS/PDVB particles were obtained using semi-batch type emulsion polymerized using divinyl benzene as a cross-linkable monomer in the presence of seed particles. PMMA particles with size of ca. 730 nm and polydispersity of 1.03 were successfully prepared in this experiment. PS particles with size of ca. 1.5 ${\mu}m$ and polydispersity of 1.01 were prepared in this experiment. Highly crosslinked PS/PDVB particles with size of ca. 1.3 ${\mu}m$ and polydispersity of 1.00 were obtained.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 1993.10a
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• pp.443-452
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• 1993

Direct freezing tests of soybean seed by liquid nitrogen were carried out at various moisture contents and the following important conclusions were drawn from the results of temperature measurements of soybean seed and photographs of bubbles generated on its surface : 1) Assuming that the temperature gradient in a soybean seed is negligible because of its small seed size and the freezing ratio is followed the Heiss's formula, and a differential equation based on the heat energy balance was introduced . The equation was easily solved by the Runge-Kutta-Gill method and the predicted values of the temperature were in good agreement with the observed data. 2) The photographs of bubble generation during freezing showed the boiling mode was nucleate, and then the most suitable formula on the nucleate boiling heat transfer was introduced from many formulate proposed up to now by fitting the calculated values based on the formula to the observed data. The formula used for the predict on of the seed temperature was as follows: $\frac{{\partial}T_s}{\partial\theta}\;=\;-\frac{{\alpha}(T_s\;-\;T_L)^{3.3}}{W(C_s\;-\;\frac{{\delta}m(CT_s\;+\;{\sigma})}{T_s^2})}$ where C = difference of the specific heat between pure ice and water m=moisture content of soybean seed $T_s$ = seed temperature $T_L$ = Temperature of liquid nitrogen W = mass of soybean seed $\alpha$ = proportional constant $\delta$ = constant depends on variety or the type of seed $\theta$ = time $\sigma$ = latent heat of melting of pure ice This study will give important information in the hydro-freezing technique by liquid nitrogen, available as a new technique of processing agricultural products in the near future.

• The Korean Journal of Food And Nutrition
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• v.26 no.4
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• pp.670-677
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• 2013

In this study, the effects of the intake of Prunus sargentii R. seed oil on the lipid levels of mouse blood were compared to the effects of commercially available corn oils. Mice from the same purchase lot were separated into 3 groups [control (n=5), corn oil-treated group (n=5), and Prunus sargentii R. seed's oil-treated group (n=5)] of equal size. The oil-treated groups of mice were fed their respective supplemented diets for a total of 8 weeks. Prunus sargentii R. seed oil is high in palmitic acid, oleic acid, and linoleic acid. The kidney weights of mice fed Prunus sargentii R. seed oil were higher than those fed corn oil (p<0.05). The HDL-cholesterol (high density lipoprotein cholesterol) levels in mice fed Prunus sargentii R. seed oil ($124.40{\pm}20.19$mg/dl) were higher than those of mice fed corn oil (p<0.05). The total cholesterol level was highest in mice fed Prunus sargentii R. seed oil, but the LDL-cholesterol (low density lipoprotein cholesterol) level was lowest in mice fed Prunus sargentii R. seed oil. Therefore, Prunus sargentii R. seed oil may be a good resource as a natural oil material.

• Korean Journal of Food Science and Technology
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• v.31 no.6
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• pp.1410-1414
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• 1999

The granular size and shape of durian seed starch were $2.0-10.0\;{\mu}m$ and oval and polygonal. Amylose contents of durian seed, corn, sweet potato and potato starch were 28.3%, 27.5%, 20.3% and 21.7%, respectively. Blue value of durian seed (0.370) higher than that of corn (0.368), sweet potato (0.332), and potato starch (0.338). Alkali numbers of durian seed, corn, sweet potato and potato starch were 7.39, 9.02, 7.08 and 5.43, respectively. Swelling power of durian seed starch was similar to that of sweet potato starch. X-ray diffraction patterns of durian seed starch showed an A-type crystalline structure. According to pasting properties by Rapid Visco-Analyzer, the gelatinization temperature of durian seed starch $(76.6^{circ}C)$ was higher than that of corn $(73.0^{circ}C)$, sweet potato $(72.3^{circ}C)$ and potato starch $(70.2^{circ}C)$. The breakdown of durian seed starch were lower than that of corn, sweet potato and potato starch.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.49 no.2
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• pp.141-147
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• 2004

This study was carried out to know the variation of soybean seed proteins, 11S and 7S globulins, and their amino acid compositions among different colored soybean varieties, 'Danbaegkong' (yellow), 'Pureunkong' (green) 'Jinyulkong' (brown), and 'Geoumjeongkong l' (black). Soybean seed proteins showed a wide range in molecular size, but the electrophoresis patterns of total seed protein subunits showed a similarity among different colored soybean varieties. Amino acid compositions of total seed proteins were similar for all soybean varieties tested. However, soybean varieties showed low composition rates in sulfur containing amino acids. The composition rates of cysteine and methionine in the 11S globulins were higher than those of total seed proteins and 7S globulins. Glutamic acid and glycine were higher in the 11S and 7S globulins than those of total seed proteins. However, the levels of methionine and phenylalanine are high in the 11S globulins, but those of valine and lysin are slightly lower than the 7S globulins. By using HPLC, we tried to analyse the soybean seed proteins. The 11S globulin was composed of 10 major peaks whereas the 7S globulin was composed of 4 major peaks. The composition rates of 11S related proteins have a tendency to increasing during the maturing whereas those of 7S related proteins have a tendency to decreasing. Composition rates of each peaks among different colored soybean varieties suggested that soybean seed proteins are varied, although they showed similarity in the electrophoresis patterns, and understanding of this characteristics is important for the utilization of soybeans.

• Molecules and Cells
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• v.41 no.12
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• pp.1072-1080
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• 2018

A plant-specific B3 domain and AP2 domain-containing transcription factor, RAV1 acts as a negative regulator of growth in many plant species and its transcription was down-regulated by BR and ABA. In this study, we found that RAV1-overexpressing transgenic plants showed abnormally developed ovules, resulting in reduced seed size, weight, and number in a silique. Interestingly, the endogenous expression of RAV1 fluctuated during seed development; it remained low during the early stage of seed development and sharply increased in the seed maturation stage. In plants, seed development is a complex process that requires coordinated growth of the embryo, endosperm, and maternal integuments. Among many genes that are associated with endosperm proliferation and embryo development, three genes consisting of SHB1, MINI3, and IKU2 form a small unit positively regulating this process, and their expression was regulated by BR and ABA. Using the floral stage-specific RNAs, we found that the expression of MINI3 and IKU2, the two downstream genes of the SHB1-MINI3-IKU2 cascade in the seed development pathway, were particularly reduced in the RAV1-overexpressing transgenic plants. We further determined that RAV1 directly binds to the promoter of MINI3 and IKU2, resulting in their repression. Direct treatment with brassinolide (BL) improved seed development of RAV1-overexpressing plants, but treatment with ABA severely worsened it. Overall, these results suggest that RAV1 is an additional negative player in the early stages of seed development, during which ABA and BR signaling are coordinated.

• Journal of Forest and Environmental Science
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• v.38 no.2
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• pp.102-109
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• 2022

This study was conducted to test the significant difference of fertility variation among families and to select superior families for acorn production in the breeding seedling seed orchards (BSSOs) of Quercus acuta and Quercus glauca. The seed orchards were located in Jeju island and established by seedlings raised from selected parents for genetic testing in 2006. In the spring of 2021, the numbers of female and male flower were counted from 5 to 10 individuals per family in the BSSOs. To test statistical significance of which parameter is not satisfied through the normality test, we used a nonparametric analysis. Correlation analysis was performed to quantify the association between female and male flower production. As the results, the significant difference of flower production among families was found in both seed orchards. The averages of female flower production were 65.3 and 181.9 in Q. acuta and Q. glauca. The positive Spearman's rank correlation was existed between male and female flower production. Broad-sense heritability on female and male flower production were 0.191 and 0.147 in Q. acuta, and 0.285 and 0.068 in Q. glauca, respectively. Sexual asymmetry (e.g., maleness index) between female and male, and contribution variation among families (e.g., parental balance) were analyzed to find reasonable alternatives in the management of seed orchards. Effective population size of seed crops was predicted as a concept of status number. Loss of gene diversity (accumulation of group coancestry) would not be alarming in the BSSOs. Our results would be helpful to select breeding materials for establishing new seed orchards and to supply genetically improved seeds of evergreen oaks, which is one of the backbones of the strategy of carbon sink in the 2050 Carbon Neutrality of Korea Forest Service.

• Korean Journal of Crystallography
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• v.5 no.2
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• pp.93-99
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• 1994

Growth form and surface morphology of NYAB single crystal grown by TSSG technique using a K2O/3MoOS/0.5B203 flux was investigated. In the crystal grown from <100> or <120> seed, prismatic and (101) faces were well developed with different size each other. (001) face was also developed in the crystal grown from <001> seed. While growth hillocks were observed on the prismatic face of the crystal grown from <100> seed, surface striations parallel to neighbor (101) faces were formed on that face of the crystal grown from <001> seed. The (101) faces were grown by two dimensional nucleation growth. (001) face which was developed at slow growth velocity of [001] direction was grown by screw dislocation Anisotropy of growth velocity as to seed orientation affected on crystal morphology and surface morphology.

• Journal of Crop Science and Biotechnology
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• v.11 no.1
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• pp.57-62
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• 2008

Isoflavone content in various parts of six soybean cultivars and soybean sprout during germination was analyzed by high performance liquid chromatography. The parts analyzed were seed coat, cotyledon, and axis for seeds and whole sprout, root, hypocotyl, and cotyledon for sprout. Two cultivars, Aga3 which is known to have the smallest seed size and the highest isoflavone content among the Korean soybean cultivars and Pungsannamulkong which is the most widely being used as soy-sprout, were selected for sampling from 1 to 10 days after germination. At the same weight, the order of isoflavone content increased from seed coat to cotyledon to axis. The highest total isoflavone(isoflavone$\times$dry weight) content was observed in the cotyledon and the lowest in the seed coat. The cotyledon of the Aga3 variety had the highest total isoflavone content and the lowest was measured in the Pungsannamulkong variety. The highest total isoflavone content, $10,788{\mu}g/g$, was observed in whole sprouts(cotyledon+hypocotyl+root) on day 7 for Aga3. After day 7, there was a decreasing trend in isoflavone content as the germination period increased. Total isoflavone content in the cotyledon of Aga3 significantly increased after seed germination, whereas the isoflavone content in the cotyledon of Pungsannamulkong decreased. However, total isoflavone content in the root of both varieties increased while isoflavone content in the hypocotyls decreased after seed germination.