• Asian Journal of Turfgrass Science
• /
• v.7 no.1
• /
• pp.19-30
• /
• 1993

Experiments were conducted to investigate propagation, shade tolerance, cold resistance and growth rate of evergreen ground cover plants ; Vinca minor K , Ajuga reptans L., Ophiopogon japonicus ker. f nanus hort , and Hedera helix.. The results were as follows : 1.It is convenient to use the growing case for rice seed to grow and transplant Vinca minor seed-ling. The most proper density of transplanting Vinea was 180 plants per 1m$^2$. At the end of growing season, the coverage came up to 90% The rooting ability of Hedera helix was best to cut from April to May (temperature 15~23˚C). The seedling length of Parthenocissus quinguefohlia were irregular and ranged from 5 cm of 200cm. 2. Ajuga reptans L., Ophiopogon j. and Vinca minor L. grew better under 50% light intensity than full sunlight. Particularly, under full sunlight and aestival high temperature, there were a few withering plants in Ajuga reptans L. , and V Vilica minor L. 3. Of Vinca minor K., Ajuga reptans L. , Ophiopogon japonicus ker. f. nanus hort. and Hedera helix. acclimatized in open field, where temperature was from -7~8˚C. There were few plants had damage in low temperature incubator to -16˚C after field acclimatization. 4.Tendrils of Parthenocissus quinguefolia strongly clung to the wire netting stone fence but intruded into the gap and cranny of the precast concrete fence and so clung to, so that it needed to install the bending net on the precast concrete fence.

• Journal of the Korean Institute of Landscape Architecture
• /
• v.15 no.2
• /
• pp.139-148
• /
• 1987

Exochorda serratifolia, broad-leaved shrub has beautiful flowers and is resistant to cold, shade and decease. New this native plant is considered to be worth being exploited as the outstanding plant for landscaping and horticulture. So this study was executed to utilize Exochorda serratifolia as the planting material for landscaping and horticulture through the survey of its habitat environment and the experimennt of its seed physiology and germination, vegetative propagation, culture and utilization, etc.. The results are as follows ; 1. The color of the flower is white, blooming in the early and middle of May and the seeds ripen late in Sep.. 2. The elevation, elevation, gradient and direction of the native habitat were 250m, 20-25$^{\circ}$and northern side respectively. 3. The soil pH of the natural habitat was 5.3 and soil fertility was poor. 4. Exochorda serratifolia appeared as indicator within P. densiflora community and its neighboring species were Q. mongolica, Q. dentata, R. mucronulatum, L. obtusiloba, E. oxyphillus, C. heterolphylla, var thunbergii, etc.. 5. The optimum temperature for seed germination was found at 20$^{\circ}C$ and the longer the stratification period at 5$^{\circ}C$ was, the lower the germination rate was. 6. The treat merits of GA and Kinetin increased the seed germination rate, especially under the dark condition but their high concentrations decreased the seed germination rate. 7. The rooted rate of the greenwood cutting was the highest at IBA 100ppm plot of vermiculite bed but its high concentration decreased the rooted rate conspicuously. 8. In the tissue culture, the each 1.0 ppd plot of NAA, Kinetin and NAA 1.0ppm + Kinetin showed the best growth. And the mixture of NAA 1.0ppm and Kinetin showed better growth than the single treatment of NAA or Kinetin did. 9. Transplanted Exochorda serratifolia showed healthy growth with shaded environmental condition(42.1% light intensity), therefore they can be cultivated as a shade tolerant landscape plant. 10. It was considered that Exochorda serratifolia was applicable to group planting at shade places or under trees in parks, homes, etc..

• Journal of Plant Biotechnology
• /
• v.43 no.1
• /
• pp.138-145
• /
• 2016

To find the optimal propagation condition for endangered Cypripedium macranthos Sw., also known as lady's slipper orchid, the effect of various organic additives on in vitro germination, protocorm formation and seedling growth was investigated during asymbiotic seed culture. When $100ml{\cdot}L^{-1}$ coconut water was added to the basal medium, the highest germination rate and protocorm formation rate were achieved, with 70.8% and 74.2% respectively. Supplementation of phloem sap from birch tree or maple tree also showed a facilitating effect to improve the germination and protocorm development. With $100ml{\cdot}L^{-1}$ birch sap or maple sap, both the germination and protocorm formation rates were roughly more than 65% and 68%. The roots and buds of the seedlings grew vigorously in the medium containing $100ml{\cdot}L^{-1}$ coconut water or phloem sap, in particular, their bud formation rates increased by more than 70%. Addition of banana powder and peptone could not create a more significantly favorable culture condition, and non-addition had the worst results. Our results demonstrated that proper organic amendments such as coconut water and phloem sap might be preferred to in vitro germination and the growth of seedlings developed from the protocorm of C. macranthos Sw. during asymbiotic seed culture.

• Korean Journal of Medicinal Crop Science
• /
• v.23 no.5
• /
• pp.370-378
• /
• 2015

Background : Aruncus dioicus var. kamtschaticus, functional wild vegetable, is perennial herb and young leaves with soft texture are generally used as edible food. So the demand for the vegetable has increased recently. This study was carried out to determine the effects of temperature and shading on germination characteristics of A. dioicus. Methods and Results : The experiment was performed by temperature and shading treatments. Seed pre-treatment before the germination experiment was carried out by the storage in low temperature ($4^{\circ}C$) under wet condition (LTW) for 0, 15, 30, 45 and 60 days and shading treatment were 35%, 50%, and 75% under control, BA (6-benzyladenine) and $GA_3$ (gibberellic acid) condition for 24 hours. Increasing the length of the storage periods led to increases seed germination percent in low temperature ($4^{\circ}C$) under wet condition (LTW), germination rate of A. dioicus seed was the highest at $15^{\circ}C$ with 60 days of seed pre-treatment. In the case of seeds pre-treatment with LTW, the more temperature went up, the more days to 50% of Germination of Final Germination Rate ($T_{50}$) went down. As a result of surveying shading treatment, germination rate was the highest in control of 35% shading and the next higher was in control of 50% shading. Conclusions : It is concluded that the temperature and shading are important factors to produce A. dioicus. Also, We suggest these results as basic data of A. dioicus for sexual propagation.

• FLOWER RESEARCH JOURNAL
• /
• v.17 no.4
• /
• pp.324-327
• /
• 2009

The white flowered 'Eorayeon 1ho' is the first Kangwon National University cultivar of L. ${\times}$ formolonga, commonly known as seed propagating lily. Crosses were conducted from 1999 to 2003 among the selected selfing lines($S_3$) of L. longiflorum 'Lorina', L. ${\times}$ formolonga 'Augusta' and 'Raizan Herald'. A new cultivar was preliminarily selected as 'E-No.1' in 2003 for its stable upward flowering habit. The evaluation of characteristics was investigated from 2003 to 2004 in Chuncheon and Yungweol, Kangwon province. 'Eorayeon 1ho' blooms in early July at outside, with high resistance to Botrytis and having upward flowering habit. The plant height is 112.4 cm and the percentage of seed germination is 96.4%. This seed propagating cultivar shows good quality as a cut flower and low blasting rate.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.52 no.2
• /
• pp.220-227
• /
• 2007

Sweetpotato fields in Korea are highly infected with virus and virus like diseases that greatly diminish both yield and quality as indicated by field observations and laboratory tests. In order to solve this problem, there is an urgent need to produce and mass propagate virus-free planting materials for distribution to the farmers. These experiments were conducted, firstly, to determine the most appropriate culture media, nutrient solution, and cutting intervals to maintain growth and vigor of tissue cultured plantleta as mother plants for propagation in insect-proof greenhouse. And as a labor saving method, the production efficiency of plug trays for rapid propagation of stem cuttings as a source of planting materials was likewise evaluated. Results showed that plants grown in medium B supplied with 0.5 and 1.0 strength of MS nutrients had high growth rate, and 20-day cutting interval was the best. 72-plug tray was better than 128-plug. Secondly, it was to develop a technique for the production of first-generation seed roots using hydroponics cultivation system. The yield of virus-free plants propagated in the non-insect proof and open-field cultivation was 2,402 kg/10a, 6% higher than those in the insect-proof cultivation, and the rate of virus re-infection was 18% higher compared to 3.3% with insect-proof cultivation. Lastly, it was to investigate the growth performance of virus free plants in farmers' field. Differences were existed in the yield depending on the variety used, but virus free plants showed an increase of $6{\sim}24%$ over virus infected plants.

• Korean Journal of Plant Resources
• /
• v.9 no.1
• /
• pp.71-75
• /
• 1996

Experiments were conducted to investigate the proper stem number and the propagation method of Lycium Chinense Mill. from 1993 to 1994.The results obtained were summerized as follows. The most effective propagation method was stem cutting 20cm in which flowering time was 15days earlier and dry fruit weight was 30 percent heavier(91kg/10a) than in seed propagation. The optimal diameter of cutting was 0.9cm of which dry fruit weight was 227kg/10a and 28 percent heavier compared with that of 0.5cm. stem number 5 had the best top growth and the heaviest 100 fruit weight as 13.4g. Fruit number per plant was increased as 864 in stem number 5. Dry fruit yield of stem number 5 was 104kg/10a and 16 percent increased compared with that of control.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2019.10a
• /
• pp.15-15
• /
• 2019

The walnut (Juglans regia L.), a member of the Juglandaceae, is native to the mountain ranges of central Asia. This species of walnut is valued commercially for its nuts and in some areas for its timber. The seeds of walnut are recalcitrant and it has strong integument dormancy and their germination is irregular, making its natural propagation difficult. Low percentage of seed germination and long propagation cycle are the main problems of propagation. This study was conducted medium composition on in vitro plantlet regeneration from axillary buds of walnut. It has proved to be the most generally applicable and reliable method of in vitro propagation. Micropropagation culture that axillary buds are excised aseptically enables faster multiplication of plants. The axillary buds of walnut new cultivar "Sinlyeong" were cultured on two basal media which contained the different plant growth regulators depending on the respective shooting and rooting stage. After 12 weeks, the shoot generation rate was 85.3%, the shoot number and its length were 1.9/explant and 2.7 cm in the most favorable medium composition. The percentage of rooting was 25.4%. From these results, it was found the optimum basal medium and plant growth regulator for in vitro plant regeneration from axillary buds of walnut new cultivar "Sinlyeong". However, we have continued to search the other medium additives to enhance the rate of walnut root.

• Korean Journal of Environment and Ecology
• /
• v.16 no.1
• /
• pp.87-93
• /
• 2002

The tree of heaven naturalized plant grows in the various habitats of the city. The principal reason is that it is reproduced not only through sexual reproduction but through asexual reproduction. By the spreading of sucker and a bud appearance of the seed it forms a group easily inside the city habitat. The distribution of seed and spreading of the seedlings of the tree of heaven were investigated like the followings. As the result of the sexual reproduction by the seed and hangs in cluster farm, 350 individuals are germinated at the Namsan the best environment, and 50 individuals at Toegyewon Rotary These seeds are dispersed by the effect of wind and geographical factor. Because the northwest wind is blowing mainly from late autumn to early spring, it is dispersed in the south. In Toegyewon Rotary, a slant site, is dispersed mainly by down the site. Compared with width of crown, the seedling of a seed germination are distributed outside more than inside the crown and the seed is spreaded until the maximum 7.5 times of crown width at the investigated area. 1 year seedlings appeared as a group at the place of 2-4 times of the crown (in 20m from the crown, 75% distribution). Considering the distribution by the seed scattering of the tree of heaven, there is a possibility of sudden individual increase even in the deteriorated city environment. So this possibility shows that we need to consider the protection of the city soil, and the species variety decrease resulted from the successful competition to the other species.

• Korean Journal of Plant Resources
• /
• v.29 no.2
• /
• pp.235-240
• /
• 2016

Factors affecting germination of seeds in the forms of various environment and chemical compounds. The present study was aim to produced effective seed propagation method of Astilbe koreana (Kom.) Nakai which had expected high value for the cut flower, ornamental and pharmaceutical material. Seed width and length ranged 0.62, 2.22 ㎜, respectively, and weight of thousand seeds was 40.5 ㎎. As result of imbibition test, showed moisture content of fresh seed (2.57%) increased rapidly by water-soaking treatment under 24 hours, recording to maximum value of 29.8%, and expansion of the seed coat was observed. Seed germination was the best at 15℃ and light conditions (40.8%) among temperature and light conditions treated. Percent germination of seeds was improved under the low (15, 20℃) than high temperature (25, 30℃). In addition, the seed was not germinated at dark condition regardless of temperature. Seeds of A. koreana thus seemed that it had low temperature germinability conditions. To improve germination rate, seeds were submerged in various concentrations of growth regulators such as GA₃ and kinetin, and minerals as KNO₃ and KCl. As a results, KNO₃ treatment, regardless of concentrations, promoted germination compared to control. Especially, percent of germination (77.8%), germination energy (96.1%), mean germination time (11.3 days) and T₅₀ (6.5 days) were effectively improved by treatment of KNO₃ 20 mM.