• 한국작물학회지
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• 제63권2호
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• pp.158-163
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• 2018

Perilla (Perilla frutescens var.frutescens) is an annual plant of the Lamiaceae family, mainly grown for obtaining oil by press extraction after roasting the seeds. Oil yield is one of its important traits, but evaluating this yield is time-consuming, requires many seeds, and is hard to adjust to pedigrees in a breeding field. The objective of this study was to develop a method for selecting high-oil-yield lines in a breeding population without oil extraction. Twenty-three perilla cultivars were used for evaluating the oil yield and seed traits such as seed hardness, seed coat thickness, seed coat proportion and crude fat. After evaluation of the seed traits of 23 perilla cultivars, the ranges of oil yields, seed hardness, seed coat thickness, seed coat proportion, 100-seed weight, and crude fat were 24.68-38.75%, 157-1166 gf, $24-399{\mu}m$, 15.4-41.5%, 2.79-6.69 g, and 33.0-47.8%, respectively. In an analysis of correlation coefficients, the oil yield negatively correlated with seed length, seed width, the proportion of seed coat, seed hardness, and 1000-seed weight, but positively correlated with crude fat content. It was observed that as the seed coat proportion increased, the seed coat thickness, hardness, and 1000-seed weight also increased. Multiple linear regression (MLR) was employed to find major variables affecting the oil yield. Among the variables, traits crude fat content and seed coat proportion were assumed to be indirect parameters for estimating the potential oil yield, with respect to a significant positive correlation with the observed oil yield ($R^2=0.791$). Using these two parameters, an equation was derived to predict the oil yield. The results of this study show that various seed traits in 23 perilla cultivars positively or negatively correlated with the oil yield. In particular, crude fat and the seed coat proportion can be used for predicting the oil yield with the newly developed equation, and this approach will improve the efficiency of selecting prominent lines for the oil yield.

• 한국작물학회지
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• 제39권1호
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• pp.55-62
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• 1994

쌀보리의 전분함량 변이와 유전율을 밝히고, 이들 특성이 어떤 형질들과 상호 관련성이 있는가를 구명하여 효율적인 육종사업을 수행하기 위한 기초자료를 얻을 목적으로 본 연구를 수행하였다. 수원시 권선구 탑동 소재의 맥류연구소포장에서 1989년과 1990년에 수확된 쌀보리의 교배모본 137품종 및 계통을 재료로 전분함량 및 이와 관련된 형질들을 조사하였고, 전분의 유전율은 쌀보리 지역적응시험에 공시된 계통 성적을 이용하여 분석하였다. 얻어진 결과를 요약하면 다음과 같다. 1. 전분함량은 최저 49.15%, 최고 62.50%, 평균 57.20%이었으며 육성종과 병성밀수품종이 활성이나 병성소수품종, 도입종보다 높았다. 2. 종피중은 1.12mg에서 3.02mg까지 분포되고 평균 1.88mg이었으며 종실에 대한 종피비율은 4.0%에서 8.7%까지, 평균 6.5%였으며 품종간 차이가 컸다. 3. 단백질함량은 9.60∼15.90%의 변이를 나타내고 평균 12.86%였다. 4. 전분함량은 입중, 종피중, 종피비율, 입폭, 입후, 단백질함량과는 부의 상관을 보였으며, 동일 품종 내에서도 입의 크기가 작은 것이 전분함량이 높아지는 경향이었다. 5. 종피중과 종피비율은 입폭, 입후와는 정의 상관이 있었고 전분함량과는 부의 상관관계가 있었다. 6. 전분함량의 유전율은 30.0%였다.

• 대한본초학회지
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• 제32권6호
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• pp.17-22
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• 2017

Objectives : Daily Dose of Apricot Kernel in Treatise on Cold Damage Diseases is usually written in the number, sometimes in the volume. The seed coat and acute end of Apricot Kernel must be removed, so author want to know its daily dose and proportion of seed coat and acute end. Methods : Assuming dosage by editions of Treatise on Cold Damage Diseases. And comparing it with measured weight of Apricot Kernel distributed in market. Results : The number of prescriptions including Apricot Kernel is ten, and eight of that are made to decoction, two of that are made to pill prescription. And two of decoction are made by reducing and uniting prescriptions. The daily dose of six decoction are 70, 47 or 35 in numbers. The 70 Apricot Kernel except seed coat and acute end are 1/2 Sheong ($33m{\ell}$) in volume, 3 Ryang (19.5 g) in weight. Weight of Apricot Kernel the most common in market is 0.28 ~ 0.38 g. 70 Apricot Kernel are 23.10 g, seed coats of that are 1.15 g, acute ends of that are 2.43 g, 70 Apricot Kernel except seed coat and acute end is 19.5 g. So, seed coat is 5%, acute end is 10% by proportion, which is the same with it assumed based on writings. Conclusions : 70 Apricot Kernel except seed coat and acute end are 1/2 Sheong, 3 Ryang, and it is 33 mL and 19.5 g respectively. It also correspond with current market goods.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2021년도 춘계학술대회
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• pp.15-15
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• 2021

Seed coat color and seed weight are among the key agronomical traits that determine the nutritional quality of soybean seeds. This study aimed to evaluate the contents of total protein, total oil and five prominent fatty acids in seeds of 49 soybean varieties recently cultivated in Korea, and assess the influences of seed coat color and seed weight on each. Total protein and total oil contents were in the ranges of 36.28-44.19% and 13.45-19.20%, respectively. Likewise, individual fatty acid contents were in the ranges of 9.90-12.55, 2.45-4.00, 14.97-38.74, 43.22-60.26, and 5.37-12.33% for palmitic, stearic, oleic, linoleic, and linolenic acids, respectively. Our results found significant variations of protein, oil and fatty acid contents between the soybean varieties. Moreover, both seed coat color and seed weight significantly affected total oil and fatty acid contents. Total protein content, however, was not significantly affected by any factor. Among colored soybeans, pale-yellow soybeans were characterized by a high level of oleic acid (30.70%) and low levels of stearic (2.72%), linoleic (49.30%) and linolenic (6.44%) acids, each being significantly different from the rest of colored soybeans (p < 0.05). On the other hand, small soybeans were characterized by high levels of all individual fatty acids except oleic acid. The level of oleic acid was significantly high in large seeds. Cluster analysis grouped the soybeans into two classes with notable content differences. Principal component analysis also revealed fatty acids as the prime factors for the variability observed among the soybean varieties. As expected, total oil and total protein contents showed a negative association with each other (r = -0.714, p < 0.0001). Besides, oleic acid and linoleic acid showed a tradeoff relationship (r = -0.936, p < 0.0001) which was reflected with respect to both seed coat color and seed weight. In general, the results of this study shade light on the significance of seed coat color and seed weight to distinguish soybeans in terms of protein, oil and fatty acid contents. Moreover, the soybean varieties with distinct characteristics and nutritional contents identified in this study could be important genetic resources for consumption and cultivar development.

• 한국작물학회지
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• 제43권4호
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• pp.269-272
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• 1998

Characteristics of seed and plant growth of the nine local collections of the medicinal plant, Agastache rugosa were evaluated. Seed size of the collection from Garyungsan was larger than the others. Seeds of Damyang collection were the smallest in size. Seeds of the collections were mostly oval shaped. There were no differences between brown and black seed coat color groups in seed width, ratio of seed length to seed width and 1,000 seed weight. Stem lengths of Garyungsan and Bongpyung collections were 99.9 and 95.6 cm, respectively, and were longer than Damyang and Jindo collections. Garyungsan collection had long leaf shape. Leaf size was the smallest for Damyang collection, but was the largest for Bongpyung collection. Inflorescence length of Mokpo collection (15.6 cm) was longer than the average over all collections (12.3 cm). Damyang collection was the shortest (10.1 cm) in inflorescence. Top dry weight of each collection from Soonchun, Bongpyung, Mokpo, Jindo, and Gurye was greater than the average over all collections (20.38 g/plant). Jinju and Damyang collections had less top dry matter than others. There was no difference in the growth traits examined between brown and black seed coat groups. The brown seed group was greater in leaf weight than the black seed group. However, these two groups were not different in stem and inflorescence weight. Collections of A. rugosa from Bongpyung, Soonchun, and Mokpo were promising for commercial cultivation because of their great top dry weight, especially in leaf and inflorescence.

• 한국육종학회지
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• 제41권4호
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• pp.603-606
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• 2009

Lipoxygenase and Kunitz trypsin inhibitor protein of mature soybean [Glycine max (L.) Merr.] seed are main anti-nutritional factors in soybean seed. A new soybean cultivar, "Gaechuck#1" with the traits of black seed coat, green cotyledon, lipoxygenase2,3 and Kunitz trypsin inhibitor protein free was developed. It was selected from the population derived the cross of "Gyeongsang#1" and C242. Plants of "Gaechuck#1" have a determinate growth habit with purple flowers, brown pubescence, black seed coat, black hilum, oval leaflet shape and brown pods at maturity. Seed protein and oil content on dry weight basis have averaged 39.1% and 16.2%, respectively. It has shown resistant reaction to soybean necrosis, soybean mosaic virus, Cercospora leaf spot and blight, black root rot, pod and stem blight, and soybean pod borer. "Gaechuck#1" matured on 5-10 October with a plant height of 50 cm. The 100-seed weight of "Gaechuck#1" was 23.2g. Yield of "Gaechuck#1" was averaged 2.2 ton/ha from 2005 to 2007.

• 한국작물학회지
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• 제39권4호
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• pp.382-388
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• 1994

저장기간과 용도에 따른 대두종실의 건물 및 화학성분의 변화를 비교하기 위하여 장려 3품종에 대하여 2년의 저장기간 차이를 두었으며 용도에 따라서는 장려 5품종과 재래 2품종을 공시재료로 하여 종실을 종피와 자엽(유근포함)으로 분리하여 건물중, 발아율, 단백질, 지방, 전분, 당 및 oligo당을 분석하였다. 1. 3년 저장된 종자에서 건물중의 감소가 나타났으며, 발아율도 급격히 저하되었다. 2. 저장기간동안 자엽의 무게가 현저히 감소하였고, 종피의 구성비율은 증가하였다. 3. 3년 저장된 종자의 종피에서는 단백질, 지방, 탄수화물이 모두 감소하였고, 자엽에서는 단백질의 함량이 41.65%로 현저하게 감소하였다. 4. 종피의 구성비율은 대립종보다 소립종에서 높았다. 5. 밥밑콩에서 자엽의 단백질과 지방함량은 평균 43.59%와 18.25%로 장려품종에 비해 낮았고, 전분과 당의 함량은 평균 5.70%와 11.58%로 현저하게 높았다. 6. Sucrose, raffinose 및 stachyose의 함량은 밥밑콩에서 높았다.

• Journal of Crop Science and Biotechnology
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• 제11권1호
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• pp.57-62
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• 2008

Isoflavone content in various parts of six soybean cultivars and soybean sprout during germination was analyzed by high performance liquid chromatography. The parts analyzed were seed coat, cotyledon, and axis for seeds and whole sprout, root, hypocotyl, and cotyledon for sprout. Two cultivars, Aga3 which is known to have the smallest seed size and the highest isoflavone content among the Korean soybean cultivars and Pungsannamulkong which is the most widely being used as soy-sprout, were selected for sampling from 1 to 10 days after germination. At the same weight, the order of isoflavone content increased from seed coat to cotyledon to axis. The highest total isoflavone(isoflavone$\times$dry weight) content was observed in the cotyledon and the lowest in the seed coat. The cotyledon of the Aga3 variety had the highest total isoflavone content and the lowest was measured in the Pungsannamulkong variety. The highest total isoflavone content, $10,788{\mu}g/g$, was observed in whole sprouts(cotyledon+hypocotyl+root) on day 7 for Aga3. After day 7, there was a decreasing trend in isoflavone content as the germination period increased. Total isoflavone content in the cotyledon of Aga3 significantly increased after seed germination, whereas the isoflavone content in the cotyledon of Pungsannamulkong decreased. However, total isoflavone content in the root of both varieties increased while isoflavone content in the hypocotyls decreased after seed germination.

• 한국육종학회지
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• 제41권4호
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• pp.612-615
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• 2009

Lipoxygenase and Kunitz trypsin inhibitor protein are the main antinutritional factor in mature soybean seed. A new soybean cultivar, "Gaechuck#2" with yellow seed coat, lipoxygenase2,3-free and Kunitz trypsin inhibitor protein-free was developed. It was selected from the population derived from the cross between "Jinpumkong2ho" and C242. Plants of "Gaechuck#2" have determinate growth habit with purple flowers, tawny pubescence, yellow seed coat, yellow hilum, oval leaflet shape and brown pods at maturity. Seed protein and oil content on a dry weight basis were 40.7% and 18.7%, respectively. It has shown a resistant reaction to soybean necrosis, soybean mosaic virus, Cercospora leaf spot and blight, black root rot, pod and stem blight, and soybean pod borer. Gaechuck#2 matured in 4 October with plant height of 54cm and a 100-seed weight of 24.4g. Average Yield of Gaechuck#2 was 230 - 250 kg/10a in 2005 - 2007.

• Journal of Plant Biotechnology
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• 제47권2호
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• pp.118-123
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• 2020

The black seed coat of soybeans contain anthocyanins which promote health. However, mature soybean seeds contain anti-nutritional factors like lipoxygenase, lectin and Kunitz Trypsin Inhibitor (KTI) proteins. Furthermore, these seeds can be used only after the genetic elimination of these proteins. Therefore, the objective of this study was to develop novel soybean genotypes with black seed coat and triple recessive alleles (lx1lx1lx2lx2lx3lx3, titilele) for lipoxygenase, lectin, and KTI proteins. From a cross of parent1 (lx1lx2lx3/lx1lx2lx3, ti/ti, Le/Le) and parent2 (lx1lx2lx3/lx1lx2lx3, Ti/Ti, le/le), 132 F₂ seeds were obtained. A 3:1 segregation ratio was observed during F₂ seed generation for the inheritance of lectin and KTI proteins. Between a cross of the Le and Ti genes, the observed independent inheritance ratio in the F₂ seed generation was 9: 3 : 3 : 1 (69 Le_Ti_: 32 leleTi_: 22 Le_titi: 9 leletiti) (χ²=2.87, P=0.5 - 0.1). From nine F₂ seeds with triple recessive alleles (lx1lx1lx2lx2lx3lx3, titilele genotype), one novel strain posessing black seed coat, and free of lipoxygenase, lectin and KTI proteins, was selected. The seed coat color of the new strain was black and the cotyledon color of the mature seed was green. The weight of 100 seeds belonging to the new strain was 35.4 g. This black soybean strain with lx1lx1lx2lx2lx3lx3, titilele genotype is a novel strain free of lipoxygenase, lectin, and KTI proteins.